FastQCFastQC Report
Fri 27 May 2016
SRR2138850_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138850_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7139918
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101350.053.31081
GTATCAA186700.038.685931
TCAACGC237550.030.0149174
ATCAACG237700.030.014023
CAACGCA245400.029.03735
TATCAAC247900.028.9103372
AACGCAG249100.028.6404386
ACGCAGA282800.025.2123157
CGCAGAG288300.024.7610938
GCAGAGT329150.021.6110619
GAGTACT196500.020.29407312-13
AGAGTAC298900.019.829310-11
TACATGG216500.019.6439572
GTACATG222700.019.578831
GTGGTAT48500.019.5552671
CAGAGTA324050.019.11116810-11
TGGTATC49050.018.5485552
ACATGGG222250.018.5306473
AGTACTT212950.018.4443412-13
GTACTTT218500.018.17283214-15
Produced by FastQC (version 0.11.5)