##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138850_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7139918
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.96142056533422	34.0	31.0	34.0	31.0	34.0
2	33.06921424027559	34.0	33.0	34.0	31.0	34.0
3	33.12967795988693	34.0	33.0	34.0	31.0	34.0
4	36.48791512731659	37.0	37.0	37.0	35.0	37.0
5	36.45296864753909	37.0	37.0	37.0	35.0	37.0
6	36.48293677882575	37.0	37.0	37.0	35.0	37.0
7	36.441103665336215	37.0	37.0	37.0	35.0	37.0
8	36.426574927050986	37.0	37.0	37.0	35.0	37.0
9	38.22086234043584	39.0	39.0	39.0	37.0	39.0
10-11	38.17990843872437	39.0	39.0	39.0	37.0	39.0
12-13	38.15127981861977	39.0	39.0	39.0	37.0	39.0
14-15	39.62077393605921	41.0	40.0	41.0	37.0	41.0
16-17	39.60199045703326	41.0	40.0	41.0	37.0	41.0
18-19	39.61605231320584	41.0	40.0	41.0	37.0	41.0
20-21	39.595582676248824	41.0	40.0	41.0	37.0	41.0
22-23	39.52102574335211	41.0	39.5	41.0	37.0	41.0
24-25	39.36861043865134	41.0	39.0	41.0	36.0	41.0
26-27	39.3748282842464	41.0	39.0	41.0	36.5	41.0
28-29	39.29624206091701	41.0	39.0	41.0	36.0	41.0
30-31	39.20567697181241	41.0	39.0	41.0	36.0	41.0
32-33	39.004306281209935	40.5	39.0	41.0	35.0	41.0
34-35	38.926477306381415	40.0	38.5	41.0	35.0	41.0
36-37	39.11712302744282	41.0	39.0	41.0	35.0	41.0
38-39	39.186920414384325	41.0	39.0	41.0	35.0	41.0
40-41	39.16584817424397	41.0	39.0	41.0	35.0	41.0
42-43	39.018673265567486	41.0	39.0	41.0	35.0	41.0
44-45	39.04370938944798	41.0	39.0	41.0	35.0	41.0
46-47	38.99766549844314	40.0	39.0	41.0	35.0	41.0
48-49	38.933437811531064	40.0	39.0	41.0	35.0	41.0
50-51	38.864626224974245	40.0	38.5	41.0	35.0	41.0
52-53	38.751375772751814	40.0	38.0	41.0	35.0	41.0
54-55	38.62078442651763	40.0	38.0	41.0	35.0	41.0
56-57	38.47689176022668	40.0	38.0	41.0	34.5	41.0
58-59	38.31979710013461	40.0	37.0	41.0	34.0	41.0
60-61	38.14023007257313	40.0	37.0	41.0	34.0	41.0
62-63	37.917462297183796	39.5	36.0	41.0	34.0	41.0
64-65	37.649942752929306	39.0	36.0	41.0	33.5	41.0
66-67	37.33678430173664	39.0	35.0	40.5	33.0	41.0
68-69	37.011729896701084	38.0	35.0	40.0	33.0	41.0
70-71	36.65758463527666	37.0	35.0	40.0	32.5	41.0
72-73	36.321818875596705	37.0	35.0	39.0	32.0	41.0
74-75	35.984540040808724	36.0	35.0	39.0	32.0	40.5
76-77	35.12346361299072	35.0	34.0	38.0	31.0	39.0
78-79	35.338963715910054	35.0	34.5	37.0	32.0	39.0
80-81	35.11740610510498	35.0	34.0	37.0	32.0	39.0
82-83	34.84616277072547	35.0	34.0	37.0	31.5	39.0
84-85	34.58022667101747	35.0	34.0	36.0	31.0	37.5
86-87	34.35941175203338	35.0	34.0	36.0	31.0	37.0
88-89	34.15754924946191	35.0	34.0	35.5	31.0	37.0
90-91	33.957255164045165	35.0	34.0	35.0	31.0	36.0
92-93	33.82330195638542	35.0	34.0	35.0	31.0	36.0
94-95	33.74461217348126	35.0	34.0	35.0	31.0	36.0
96-97	33.715784501484926	35.0	34.0	35.0	31.0	36.0
98-99	33.733005753921034	35.0	34.0	35.0	31.0	35.0
100-101	32.35807958262233	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	1.0
18	0.0
19	4.0
20	28.0
21	101.0
22	479.0
23	1609.0
24	4279.0
25	9107.0
26	16802.0
27	27650.0
28	40828.0
29	57483.0
30	75945.0
31	98053.0
32	126843.0
33	169953.0
34	245858.0
35	375092.0
36	673694.0
37	1526524.0
38	2344230.0
39	1268593.0
40	76761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.18677205911497	21.2382720975608	15.610587630080294	23.964368213243937
2	13.572470424959556	21.702298404343924	41.58739850184044	23.137832668856078
3	16.696958802182156	26.70231320616192	31.505184163905547	25.095543827750376
4	11.732629384907415	18.943497522450194	39.4701927207277	29.853680371914688
5	12.808538250514676	38.05906987434168	35.87950672255714	13.252885152586508
6	29.065898104977972	38.75008631052714	18.98897424157732	13.195041342917571
7	25.835507262412406	33.30436473044353	23.30446837314847	17.55565963399559
8	24.339082307091026	36.69638564105322	21.311091512283884	17.653440539571864
9	25.245980990439477	17.077623562633608	21.593329076493013	36.083066370433905
10-11	23.66936847173875	27.293772841648885	29.117162129873197	19.919696556739165
12-13	24.687720503232672	24.79624415854636	29.27506310296561	21.240972235255363
14-15	21.81786400905405	26.21934408028582	27.178566769071615	24.784225141588518
16-17	21.160260798732534	29.432204486553896	27.75906061587314	21.648474098840435
18-19	21.177834255239347	28.379212478350592	29.758948212010278	20.684005054399783
20-21	22.058844538615478	27.457761696243892	29.687958700947707	20.795435064192922
22-23	21.76086455152587	27.39754675156197	29.558666431624562	21.282922265287592
24-25	21.545386515795652	27.566159066154967	29.56936601853521	21.319088399514172
26-27	21.459327476103606	27.728605444887144	29.515320512294906	21.29674656671434
28-29	21.342868958067257	27.81479798803851	29.592445579704847	21.249887474189393
30-31	21.7317240557782	27.625899290705448	29.41794027755053	21.224436375965823
32-33	21.53840259913528	27.793818904773698	29.323031202224243	21.344747293866774
34-35	21.585868468571086	27.83106962330416	29.374868656673808	21.20819325145094
36-37	21.539985130806752	27.801849404612426	29.348195914903446	21.30996954967738
38-39	21.404649191990586	27.858092289505404	29.39147464166113	21.34578387684288
40-41	21.678756412855886	27.761528959633896	29.191822055017397	21.36789257249282
42-43	21.67922163113741	27.861552045385622	28.953552039040726	21.505674284436243
44-45	21.737494423929675	27.988611910231718	28.736850562769554	21.53704310306905
46-47	21.794478019700577	27.961116943244914	28.44331859827208	21.801086438782434
48-49	21.746866143079256	28.115981408429537	28.416116578210026	21.721035870281185
50-51	21.750579643823553	28.22883354769519	28.256930244569787	21.763656563911464
52-53	21.91280186449587	28.138867999060917	28.166515083166686	21.781815053276528
54-55	21.923173629338642	28.054912895071908	28.17388259472536	21.84803088086409
56-57	21.93355500823026	28.198895029313427	28.046427296096187	21.82112266636012
58-59	21.84744144223708	28.28316386432646	28.12158242383566	21.747812269600793
60-61	21.94055967827291	28.249269033394516	28.06881462705533	21.74135666127725
62-63	21.869662210942334	28.33029669833796	28.008761424978534	21.791279665741172
64-65	21.90762238541067	28.284886269659033	27.989298755707843	21.818192589222456
66-67	21.950679699644716	28.267247060571904	27.85208278952631	21.929990450257066
68-69	21.92087570155054	28.210653577792023	27.952135369417476	21.916335351239972
70-71	21.92615396019228	28.029094706370785	27.972119252794155	22.07263208064278
72-73	21.97106224966677	28.070062459022953	27.958542552793524	22.000332738516747
74-75	21.843832654448786	28.190725629579244	27.91704124126335	22.048400474708618
76-77	21.981428728801703	28.121536044858843	27.839755753080457	22.057279473258998
78-79	21.95390538737253	28.15726639554837	27.84911193830732	22.03971627877178
80-81	21.95424966936525	28.093189785625178	27.853024346283117	22.09953619872645
82-83	21.991785038862304	28.07084076652055	27.92746335494007	22.009910839677076
84-85	22.11983898832478	28.014576930631108	27.89183666625875	21.973747414785358
86-87	21.90822774276731	28.004710342800454	28.0162634156977	22.070798498734533
88-89	22.023545298879064	27.96649328334923	27.926558511924565	22.083402905847137
90-91	22.077885272597914	27.93871715199689	27.984368178689202	21.999029396715997
92-93	22.087835253074545	27.84556714579356	27.99318557457705	22.073412026554845
94-95	21.934506347235377	27.8867114331761	28.05157046918297	22.127211750405554
96-97	21.93233836072314	27.923534272101058	28.127963486725942	22.016163880449863
98-99	22.111239733741353	28.49233148829017	28.12978883779924	21.26663994016924
100-101	19.935826492960175	32.20664046322236	26.349454493410985	21.508078550406484
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1748.0
1	1539.0
2	2003.5
3	2814.0
4	3766.0
5	5682.5
6	7303.0
7	9047.0
8	10661.5
9	11011.5
10	10276.5
11	9604.5
12	10767.0
13	14486.5
14	23507.0
15	35648.5
16	45031.5
17	49735.5
18	49782.5
19	46795.5
20	42419.5
21	37912.0
22	35558.5
23	37359.0
24	43353.5
25	53713.5
26	67458.5
27	82843.5
28	100052.0
29	117617.5
30	133337.5
31	149824.0
32	166947.0
33	183789.5
34	200400.5
35	214536.0
36	230200.0
37	244266.5
38	253457.0
39	260520.5
40	268764.0
41	277528.5
42	281378.0
43	284226.5
44	287672.0
45	290744.0
46	291335.5
47	288620.0
48	284132.5
49	277116.5
50	268971.5
51	258039.0
52	243198.0
53	228963.0
54	215801.0
55	200528.0
56	183914.0
57	165405.0
58	145041.0
59	125065.0
60	105668.5
61	86450.5
62	67901.0
63	51952.0
64	38809.5
65	28858.0
66	21983.0
67	16799.0
68	12728.0
69	9074.5
70	6297.5
71	4542.5
72	3227.5
73	2251.0
74	1580.0
75	1058.5
76	645.5
77	410.0
78	283.5
79	190.0
80	129.5
81	82.5
82	53.5
83	34.5
84	26.5
85	18.5
86	10.5
87	9.5
88	9.0
89	6.5
90	4.0
91	2.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.4005763091396848E-5
2	0.03119083440454078
3	1.4005763091396848E-5
4	1.4005763091396848E-5
5	1.4005763091396848E-5
6	1.4005763091396848E-5
7	5.602305236558739E-5
8	4.902017081988897E-4
9	5.182132343816833E-4
10-11	0.0
12-13	0.0
14-15	0.0017717290310617015
16-17	8.40345785483811E-5
18-19	0.0
20-21	2.1709463372178567E-4
22-23	0.0018069060368940799
24-25	7.355019948215055E-4
26-27	0.014805922593263299
28-29	0.312782756972411
30-31	0.31346857948588347
32-33	0.3310172276911631
34-35	0.2139233732808797
36-37	0.07404316376670174
38-39	0.06577960395312432
40-41	0.025984511541304704
42-43	0.009783162017453825
44-45	0.012970065829627281
46-47	0.009118786734411959
48-49	0.006225560506111388
50-51	0.006978851986320255
52-53	0.002943014360254162
54-55	0.001076705892629372
56-57	0.0032253513225392596
58-59	0.003860789635513716
60-61	0.00262613509444957
62-63	0.00158219482536133
64-65	4.8182222955574965E-4
66-67	0.0077090411746506
68-69	0.01183249152266377
70-71	0.012092613818476865
72-73	0.01037649278973198
74-75	0.010091590079445401
76-77	0.009594303056621265
78-79	0.002650394035094087
80-81	0.002243131390952129
82-83	0.0017496306167775443
84-85	8.709924331661377E-6
86-87	0.002932210722222873
88-89	0.0
90-91	0.008456187203559649
92-93	0.019151035423193505
94-95	0.0014484729427575564
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	100.0
20-21	383.0
22-23	833.0
24-25	2293.0
26-27	3310.0
28-29	5759.0
30-31	13002.0
32-33	17468.0
34-35	25136.0
36-37	35383.0
38-39	42369.0
40-41	45783.0
42-43	54592.0
44-45	66383.0
46-47	65906.0
48-49	56840.0
50-51	49712.0
52-53	47935.0
54-55	51322.0
56-57	53757.0
58-59	53183.0
60-61	52077.0
62-63	52559.0
64-65	56695.0
66-67	60522.0
68-69	59588.0
70-71	57662.0
72-73	55622.0
74-75	56750.0
76-77	58132.0
78-79	60979.0
80-81	61608.0
82-83	60623.0
84-85	61394.0
86-87	65219.0
88-89	70456.0
90-91	75445.0
92-93	76967.0
94-95	86940.0
96-97	136540.0
98-99	616435.0
100-101	4566256.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.06474737083943
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.99459802222533	77.4536234352307
2	7.155949922814149	12.317500446107553
3	1.6631449471421744	4.294144491506381
4	0.5718061020053037	1.968493908567636
5	0.25041403332308165	1.0775910258031993
6	0.12389574473536542	0.639783358258274
7	0.07534945435896723	0.4539452227754577
8	0.04658969717545033	0.3207784413991234
9	0.030268921138211387	0.23445783458532224
>10	0.08650651842741792	1.0985373076717726
>50	0.0012005172108442911	0.06479842037077203
>100	2.0258083230450963E-4	0.03609320190274784
>500	7.353861137775308E-5	0.04025290582105701
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4005763091396848E-5
2	1.4005763091396848E-5	1.4005763091396848E-5	0.0	0.0	1.4005763091396848E-5
3	1.4005763091396848E-5	1.4005763091396848E-5	0.0	1.4005763091396848E-5	1.4005763091396848E-5
4	1.4005763091396848E-5	4.201728927419055E-5	0.0	1.4005763091396848E-5	1.4005763091396848E-5
5	2.8011526182793697E-5	4.201728927419055E-5	0.0	1.4005763091396848E-5	1.4005763091396848E-5
6	4.201728927419055E-5	4.201728927419055E-5	0.0	1.4005763091396848E-5	5.602305236558739E-5
7	4.201728927419055E-5	4.201728927419055E-5	0.0	2.8011526182793697E-5	5.602305236558739E-5
8	4.201728927419055E-5	8.40345785483811E-5	0.0	2.8011526182793697E-5	7.002881545698423E-5
9	4.201728927419055E-5	1.8207492018815902E-4	0.0	4.201728927419055E-5	7.002881545698423E-5
10-11	7.703169700268266E-5	1.8207492018815902E-4	0.0	4.201728927419055E-5	7.002881545698423E-5
12-13	9.804034163977794E-5	1.8207492018815902E-4	0.0	5.602305236558739E-5	7.002881545698423E-5
14-15	9.804034163977794E-5	2.1708932791665114E-4	0.0	7.002881545698423E-5	1.0504322318547637E-4
16-17	1.2605186782257163E-4	2.31095091008048E-4	0.0	7.002881545698423E-5	1.3305474936827004E-4
18-19	1.2605186782257163E-4	2.4510085409944486E-4	0.0	7.002881545698423E-5	1.4005763091396847E-4
20-21	1.4005763091396847E-4	2.5210373564514327E-4	0.0	8.40345785483811E-5	1.470605124596669E-4
22-23	1.470605124596669E-4	2.7311238028223853E-4	0.0	8.40345785483811E-5	1.6806915709676216E-4
24-25	1.6806915709676216E-4	3.9216136655911176E-4	0.0	1.8207492018815902E-4	1.8907780173385745E-4
26-27	1.8207492018815902E-4	4.201728927419054E-4	0.0	3.291354326478259E-4	1.9608068327955588E-4
28-29	1.8207492018815902E-4	4.271757742876039E-4	0.0	8.193371408467156E-4	1.9608068327955588E-4
30-31	1.8207492018815902E-4	5.182132343816833E-4	0.0	0.0020378385297982415	1.9608068327955588E-4
32-33	2.5210373564514327E-4	5.462247605644771E-4	0.0	0.004544870123158277	1.9608068327955588E-4
34-35	2.80115261827937E-4	6.722766283870487E-4	0.0	0.008774610576760124	1.9608068327955588E-4
36-37	3.0812678801073065E-4	8.053313777553188E-4	0.0	0.014853111758426357	1.9608068327955588E-4
38-39	3.0812678801073065E-4	8.473486670295093E-4	0.0	0.023137520626987595	2.0308356482525428E-4
40-41	3.0812678801073065E-4	9.874062979434777E-4	0.0	0.031582995771099895	2.100864463709527E-4
42-43	3.0812678801073065E-4	0.001008414942580573	0.0	0.03957328361474179	2.100864463709527E-4
44-45	3.151296695564291E-4	0.0010714408764918588	0.0	0.0488941189520664	2.1708932791665114E-4
46-47	3.361383141935243E-4	0.0011204610473117477	0.0	0.05856509836667592	2.2409220946234957E-4
48-49	3.501440772849212E-4	0.0011204610473117477	0.0	0.06771086166535806	2.941210249193338E-4
50-51	3.6414984037631804E-4	0.0012675215597714147	0.0	0.07610731663865047	3.0812678801073065E-4
52-53	3.6414984037631804E-4	0.0013305474936827005	0.0	0.08431469381020903	3.221325511021275E-4
54-55	3.6414984037631804E-4	0.0013445532567740973	0.0	0.09277417471741273	3.431411957392228E-4
56-57	3.6414984037631804E-4	0.0013585590198654943	0.0	0.09891570183299024	3.7115272192201644E-4
58-59	3.6414984037631804E-4	0.0013585590198654943	0.0	0.10397878519053019	3.781556034677149E-4
60-61	3.6414984037631804E-4	0.0013935734275939862	0.0	0.10906988007425296	3.781556034677149E-4
62-63	3.6414984037631804E-4	0.0014285878353224785	0.0	0.11363575884204832	3.9216136655911176E-4
64-65	3.6414984037631804E-4	0.0016246685186020343	0.0	0.11836970676694046	3.9216136655911176E-4
66-67	3.9216136655911176E-4	0.001652680044784828	0.0	0.12360786216312289	3.9216136655911176E-4
68-69	3.9216136655911176E-4	0.0016946973340590185	0.0	0.12881100315157681	4.201728927419054E-4
70-71	3.9216136655911176E-4	0.0017087030971504155	0.0	0.1336429914181087	4.341786558333023E-4
72-73	3.9216136655911176E-4	0.0017227088602418123	0.0	0.1372564782956891	5.042074712902865E-4
74-75	3.9216136655911176E-4	0.0017647261495160028	0.0	0.14028172312343085	5.042074712902865E-4
76-77	3.9216136655911176E-4	0.0018277520834272887	0.0	0.14326495066189837	5.042074712902865E-4
78-79	3.9216136655911176E-4	0.001848760728064384	0.0	0.14575797649216699	5.042074712902865E-4
80-81	3.9216136655911176E-4	0.001848760728064384	0.0	0.1470605124596669	5.462247605644771E-4
82-83	3.9216136655911176E-4	0.001848760728064384	0.0	0.1475367084047744	5.882420498386676E-4
84-85	3.9216136655911176E-4	0.0018627664911557808	0.0	0.14783783231123943	5.882420498386676E-4
86-87	3.9216136655911176E-4	0.0018767722542471776	0.0	0.1479568812975163	5.882420498386676E-4
88-89	3.9216136655911176E-4	0.0019888183589783525	0.0	0.1480129043498819	5.882420498386676E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10135	0.0	53.3108	1
GTATCAA	18670	0.0	38.68593	1
TCAACGC	23755	0.0	30.014917	4
ATCAACG	23770	0.0	30.01402	3
CAACGCA	24540	0.0	29.0373	5
TATCAAC	24790	0.0	28.910337	2
AACGCAG	24910	0.0	28.640438	6
ACGCAGA	28280	0.0	25.212315	7
CGCAGAG	28830	0.0	24.761093	8
GCAGAGT	32915	0.0	21.611061	9
GAGTACT	19650	0.0	20.294073	12-13
AGAGTAC	29890	0.0	19.8293	10-11
TACATGG	21650	0.0	19.643957	2
GTACATG	22270	0.0	19.57883	1
GTGGTAT	4850	0.0	19.555267	1
CAGAGTA	32405	0.0	19.111168	10-11
TGGTATC	4905	0.0	18.548555	2
ACATGGG	22225	0.0	18.530647	3
AGTACTT	21295	0.0	18.44434	12-13
GTACTTT	21850	0.0	18.172832	14-15
>>END_MODULE