FastQCFastQC Report
Fri 27 May 2016
SRR2138849_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138849_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8949142
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123200.051.995761
GTATCAA234400.038.581881
TCAACGC288050.030.9846764
ATCAACG287650.030.9831143
CAACGCA295000.030.2401855
TATCAAC299700.029.9468822
AACGCAG304050.029.3964066
ACGCAGA348650.025.6114047
CGCAGAG353800.025.2385988
GCAGAGT408400.021.7819949
GTGGTAT58700.021.7382831
GAGTACT244800.019.95306812-13
TGGTATC59250.019.946552
AGAGTAC371250.019.1415510-11
CAGAGTA398250.019.03698710-11
GTACATG268200.018.9034041
GTACTTT266550.018.37347414-15
TACATGG271750.018.2310162
AGTACTT265000.017.7940212-13
ACATGGG276700.017.4505333
Produced by FastQC (version 0.11.5)