##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138849_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8949142
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95365030524714	34.0	31.0	34.0	31.0	34.0
2	33.063379148526195	34.0	33.0	34.0	31.0	34.0
3	33.12447316178467	34.0	33.0	34.0	31.0	34.0
4	36.48333728529506	37.0	37.0	37.0	35.0	37.0
5	36.44725740188277	37.0	37.0	37.0	35.0	37.0
6	36.47713255639479	37.0	37.0	37.0	35.0	37.0
7	36.43411379549011	37.0	37.0	37.0	35.0	37.0
8	36.41760785559107	37.0	37.0	37.0	35.0	37.0
9	38.20971015992371	39.0	39.0	39.0	37.0	39.0
10-11	38.16639991856202	39.0	39.0	39.0	37.0	39.0
12-13	38.13996352946461	39.0	39.0	39.0	37.0	39.0
14-15	39.608773612040125	41.0	40.0	41.0	37.0	41.0
16-17	39.5891472053969	41.0	40.0	41.0	37.0	41.0
18-19	39.60141380033974	41.0	40.0	41.0	37.0	41.0
20-21	39.580349015804174	41.0	40.0	41.0	37.0	41.0
22-23	39.5055488448601	41.0	39.0	41.0	37.0	41.0
24-25	39.353690950262916	41.0	39.0	41.0	36.0	41.0
26-27	39.35923468636176	41.0	39.0	41.0	36.0	41.0
28-29	39.27956459615203	41.0	39.0	41.0	36.0	41.0
30-31	39.19054982275643	41.0	39.0	41.0	36.0	41.0
32-33	38.992763360563615	40.5	39.0	41.0	35.0	41.0
34-35	38.91540203890362	40.0	38.5	41.0	35.0	41.0
36-37	39.10359944891515	41.0	39.0	41.0	35.0	41.0
38-39	39.17432370212005	41.0	39.0	41.0	35.0	41.0
40-41	39.15239782008185	41.0	39.0	41.0	35.0	41.0
42-43	39.003786019073985	41.0	39.0	41.0	35.0	41.0
44-45	39.028370383061045	41.0	39.0	41.0	35.0	41.0
46-47	38.9829287451295	40.0	39.0	41.0	35.0	41.0
48-49	38.92078944860987	40.0	39.0	41.0	35.0	41.0
50-51	38.85115144400483	40.0	38.0	41.0	35.0	41.0
52-53	38.73665776342788	40.0	38.0	41.0	35.0	41.0
54-55	38.604971579621825	40.0	38.0	41.0	35.0	41.0
56-57	38.46112599343671	40.0	37.5	41.0	34.0	41.0
58-59	38.30290639969371	40.0	37.0	41.0	34.0	41.0
60-61	38.12471893974129	40.0	37.0	41.0	34.0	41.0
62-63	37.89996124927555	39.0	36.0	41.0	34.0	41.0
64-65	37.63234807255942	39.0	36.0	41.0	33.5	41.0
66-67	37.317255695467445	39.0	35.0	40.5	33.0	41.0
68-69	36.99233150222063	38.0	35.0	40.0	33.0	41.0
70-71	36.63734765512491	37.0	35.0	40.0	32.5	41.0
72-73	36.29885470443169	37.0	35.0	39.0	32.0	41.0
74-75	35.96175558494791	36.0	35.0	39.0	32.0	40.5
76-77	35.10103703776895	35.0	34.0	38.0	30.5	39.0
78-79	35.31074106562262	35.0	34.5	37.0	32.0	39.0
80-81	35.08891633200669	35.0	34.0	37.0	32.0	39.0
82-83	34.81963375983467	35.0	34.0	36.5	31.5	39.0
84-85	34.55688161991087	35.0	34.0	36.0	31.0	37.0
86-87	34.33819273665289	35.0	34.0	36.0	31.0	37.0
88-89	34.137314589280024	35.0	34.0	35.5	31.0	37.0
90-91	33.94192866925785	35.0	34.0	35.0	31.0	36.0
92-93	33.81006752382169	35.0	34.0	35.0	31.0	36.0
94-95	33.73391016600557	35.0	34.0	35.0	31.0	36.0
96-97	33.70688973302575	35.0	34.0	35.0	31.0	35.5
98-99	33.72604458414136	35.0	34.0	35.0	31.0	35.0
100-101	32.34742823683741	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	0.0
15	0.0
16	0.0
17	2.0
18	2.0
19	6.0
20	24.0
21	113.0
22	632.0
23	2263.0
24	5870.0
25	12546.0
26	22271.0
27	36275.0
28	53411.0
29	73476.0
30	96184.0
31	123661.0
32	159440.0
33	212019.0
34	309575.0
35	468482.0
36	846207.0
37	1904161.0
38	2930062.0
39	1593007.0
40	99451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.103399879764986	21.332977246975688	15.688490737657473	23.875132135601856
2	13.617026746527511	21.683460209391605	41.5454940230115	23.15401902106938
3	16.714678728905792	26.708991031540464	31.497898122054185	25.07843211749956
4	11.801122789452394	18.947474282445018	39.46004867506822	29.79135425303437
5	12.94642837188825	38.002243791023496	35.82825835778634	13.223069479301921
6	29.183195256750928	38.67182768400092	18.995680031824286	13.149297027423865
7	25.96001446396613	33.25093059262928	23.312194607390033	17.47686033601456
8	24.37325645387931	36.67190184460405	21.28928408802027	17.665557613496365
9	25.29789601094904	17.096508965821798	21.594359921940722	36.01123510128844
10-11	23.746443792364406	27.189919925266565	29.148717083429244	19.91491919893979
12-13	24.74593648104734	24.78195111485573	29.250877737972587	21.221234666124342
14-15	21.921811670343256	26.199863291331045	27.041391527526613	24.83693351079908
16-17	21.282972800451443	29.350644126319438	27.66241439468329	21.703968678545838
18-19	21.28032176496046	28.34399413306819	29.648722709810922	20.72696139216043
20-21	22.192293777892626	27.40296264221311	29.56996291459182	20.834780665302446
22-23	21.89075348049629	27.33936164518064	29.392825680098873	21.377059194224195
24-25	21.68511057711734	27.5529795885702	29.41803949744426	21.343870336868203
26-27	21.6377954358451	27.66076493888822	29.358669074056305	21.34277055121037
28-29	21.498289945036174	27.750064150558234	29.356905874227195	21.394740030178397
30-31	21.864876108309332	27.529222472760388	29.28320557834191	21.322695840588374
32-33	21.66917538977811	27.750513861814024	29.142518834563152	21.437791913844713
34-35	21.72902603800339	27.782083068461638	29.171590343682475	21.317300549852494
36-37	21.677076718591252	27.726362809745158	29.149364470371626	21.447196001291964
38-39	21.579604213197847	27.773890056089474	29.204660044929092	21.44184568578359
40-41	21.851143488892582	27.69088124578511	29.002222558130335	21.455752707191973
42-43	21.822568895883858	27.76431366120571	28.766612991828133	21.6465044510823
44-45	21.891743620973216	27.907996184746064	28.560008684753324	21.640251509527396
46-47	21.947060248916276	27.897592068412198	28.27031159298315	21.885036089688377
48-49	21.873362754528415	28.073339837530796	28.221726213375714	21.831571194565072
50-51	21.90120295192161	28.14615658457501	28.096191990088247	21.85644847341513
52-53	22.07156712877962	28.0055986773191	27.999958631647953	21.922875562253328
54-55	22.029058472883737	27.982813194363032	27.981637841749674	22.006490491003554
56-57	22.051289914151514	28.107872811294904	27.912256992869978	21.9285802816836
58-59	21.953776659386364	28.18608797796171	27.98526665515913	21.8748687074928
60-61	22.091855417099847	28.129154339163186	27.92178762064552	21.85720262309145
62-63	22.032619530912655	28.19897614421406	27.87593680812354	21.892467516749743
64-65	22.029200783582052	28.18943074046356	27.81138177890565	21.96998669704874
66-67	22.094655695406686	28.155619368587793	27.71829823673263	22.03142669927289
68-69	22.030010595749665	28.1178183450658	27.820536889972875	22.03163416921166
70-71	22.025897511604352	27.97492879153751	27.857769416139405	22.141404280718728
72-73	22.12195054667169	27.96289095597595	27.814889595043894	22.10026890230846
74-75	22.000669842417285	28.06414944633897	27.773409192224552	22.161771519019194
76-77	22.09384206010262	28.009567253150525	27.737212982740296	22.159377704006562
78-79	22.072839993151366	27.99121881255492	27.749886335278223	22.186054859015496
80-81	22.070440586697618	28.00411863991607	27.76179944381812	22.163641329568193
82-83	22.095964344817208	27.952058757157143	27.831779761325322	22.120197136700327
84-85	22.216799526971972	27.924066671005132	27.77787757960242	22.081256222420475
86-87	22.086764690285197	27.897411059298516	27.85157871291854	22.164245537497745
88-89	22.099784114917686	27.874577652708886	27.833450549633028	22.192187682740403
90-91	22.169906439447352	27.895173359469705	27.82565432326584	22.1092658778171
92-93	22.202370870023916	27.764238045322102	27.889274339179543	22.144116745474435
94-95	22.060356222695194	27.7710615735036	27.95147305925843	22.217109144542775
96-97	22.063550236149315	27.829145102918744	27.972273731449555	22.13503092948239
98-99	22.30433666761264	28.34086642437451	27.983781178173757	21.37101572983909
100-101	20.08206108807202	32.09465078638066	26.161178020642005	21.662110104905313
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1851.0
1	1666.5
2	2355.0
3	3396.5
4	4494.5
5	6622.0
6	8381.5
7	10296.0
8	12134.5
9	12637.0
10	11821.0
11	10966.5
12	12253.5
13	16636.0
14	27166.5
15	41620.5
16	53271.0
17	59451.5
18	59827.0
19	56212.0
20	50999.0
21	45628.5
22	42566.5
23	44417.5
24	51515.5
25	63376.5
26	79490.5
27	98052.0
28	118307.0
29	139928.0
30	160967.5
31	182134.0
32	204727.5
33	226724.5
34	247628.5
35	267115.5
36	287559.0
37	306986.5
38	322000.5
39	333725.0
40	343764.5
41	353325.0
42	358525.5
43	362044.5
44	366448.5
45	370682.0
46	372121.0
47	369435.0
48	363389.0
49	353076.0
50	341591.0
51	328622.5
52	311376.0
53	293230.5
54	275824.0
55	255258.0
56	232735.0
57	208473.0
58	183720.5
59	159086.0
60	133365.0
61	109555.5
62	87207.0
63	66815.0
64	49768.5
65	37025.5
66	27858.5
67	21102.5
68	16182.5
69	11643.5
70	8041.5
71	5723.5
72	4005.5
73	2752.5
74	1877.0
75	1271.0
76	833.5
77	520.0
78	334.0
79	212.0
80	139.5
81	89.5
82	60.5
83	39.5
84	30.0
85	24.5
86	17.5
87	11.5
88	5.5
89	3.0
90	2.5
91	2.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.234851117570824E-5
2	0.03069568009983527
3	2.234851117570824E-5
4	2.234851117570824E-5
5	2.234851117570824E-5
6	2.234851117570824E-5
7	6.704553352712472E-5
8	7.48675124386226E-4
9	5.140157570412895E-4
10-11	2.234851117570824E-5
12-13	2.234851117570824E-5
14-15	0.0020672372837530124
16-17	5.028415014534354E-5
18-19	1.117425558785412E-5
20-21	1.620301460997891E-4
22-23	0.002005945084822701
24-25	8.10354474755055E-4
26-27	0.014524084655623219
28-29	0.31605013127969456
30-31	0.3169408386246863
32-33	0.3346114660981189
34-35	0.21564109625339772
36-37	0.07271890061042303
38-39	0.06418054703522508
40-41	0.026182922550274955
42-43	0.009950803664082738
44-45	0.013141509093355446
46-47	0.009426736581629647
48-49	0.006444990174201161
50-51	0.006815059515103145
52-53	0.0026936805952601202
54-55	9.572367797270336E-4
56-57	0.003206914131300716
58-59	0.003548236988226876
60-61	0.0028080509554393373
62-63	0.0019920195187844623
64-65	6.697237661145173E-4
66-67	0.008126230336149108
68-69	0.012213965484209648
70-71	0.0122889355797603
72-73	0.010271617288471672
74-75	0.010376109779639275
76-77	0.00930014749672369
78-79	0.002414957638801889
80-81	0.0021061871161251735
82-83	0.0016731062822098884
84-85	0.0
86-87	0.0028211339558541324
88-89	7.157518296585084E-6
90-91	0.009058995237753656
92-93	0.01956137641171673
94-95	0.0016130578827898373
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	112.0
20-21	428.0
22-23	1074.0
24-25	2911.0
26-27	4221.0
28-29	7098.0
30-31	16540.0
32-33	22022.0
34-35	32094.0
36-37	45920.0
38-39	54673.0
40-41	58231.0
42-43	69372.0
44-45	84190.0
46-47	83110.0
48-49	72527.0
50-51	63517.0
52-53	61901.0
54-55	66581.0
56-57	68730.0
58-59	68729.0
60-61	66803.0
62-63	66957.0
64-65	73247.0
66-67	77283.0
68-69	76414.0
70-71	73466.0
72-73	71527.0
74-75	73313.0
76-77	75076.0
78-79	79129.0
80-81	80071.0
82-83	79008.0
84-85	80472.0
86-87	84696.0
88-89	90443.0
90-91	96714.0
92-93	98042.0
94-95	110993.0
96-97	173544.0
98-99	769209.0
100-101	5668754.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.47240683975359
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.24408685461893	75.38662812825676
2	7.411070423343593	12.5206191183749
3	1.8538083228640878	4.697869525556895
4	0.7005185461883019	2.366979505296438
5	0.31338492981096283	1.323618964421964
6	0.17022585377023522	0.8627632544594229
7	0.09313249312885025	0.5506988094706556
8	0.057327096428105274	0.3874046249933355
9	0.0375106058776848	0.2851750044454899
>10	0.11702904410803841	1.47391214820204
>50	0.001552116697972193	0.08578008148780944
>100	3.321409776274139E-4	0.04856488070502402
>500	2.1572185614378437E-5	0.009985954329229696
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.469702235141648E-5	0.0	1.117425558785412E-5	0.0
3	0.0	4.469702235141648E-5	0.0	1.117425558785412E-5	0.0
4	0.0	1.229168114663953E-4	0.0	3.352276676356236E-5	0.0
5	0.0	1.229168114663953E-4	0.0	3.352276676356236E-5	0.0
6	0.0	1.229168114663953E-4	0.0	3.352276676356236E-5	0.0
7	0.0	1.3409106705424944E-4	0.0	6.704553352712472E-5	0.0
8	0.0	1.4526532264210357E-4	0.0	6.704553352712472E-5	0.0
9	0.0	2.9053064528420714E-4	0.0	6.704553352712472E-5	0.0
10-11	0.0	2.9053064528420714E-4	0.0	7.821978911497885E-5	0.0
12-13	0.0	2.9053064528420714E-4	0.0	7.821978911497885E-5	0.0
14-15	0.0	3.8551181778096713E-4	0.0	7.821978911497885E-5	0.0
16-17	0.0	3.910989455748942E-4	0.0	7.821978911497885E-5	0.0
18-19	0.0	4.190345845445295E-4	0.0	9.498117249676002E-5	0.0
20-21	0.0	4.749058624838001E-4	0.0	1.1732968367246826E-4	5.58712779392706E-6
22-23	0.0	5.58712779392706E-4	0.0	1.396781948481765E-4	2.234851117570824E-5
24-25	0.0	5.922355461562684E-4	0.0	2.514207507267177E-4	3.352276676356236E-5
26-27	0.0	6.034098017441225E-4	0.0	3.128791564599154E-4	3.352276676356236E-5
28-29	0.0	6.034098017441225E-4	0.0	8.157206579133508E-4	3.352276676356236E-5
30-31	0.0	7.095652298287367E-4	0.0	0.002145457072867991	3.352276676356236E-5
32-33	0.0	7.151523576226637E-4	0.0	0.004385895318232742	3.352276676356236E-5
34-35	0.0	7.598493799740801E-4	0.0	0.008525957013532695	3.352276676356236E-5
36-37	0.0	8.101335301194237E-4	0.0	0.014548880775386065	3.352276676356236E-5
38-39	0.0	8.827661914404756E-4	0.0	0.022946333849658437	3.352276676356236E-5
40-41	0.0	0.0010056830029068708	0.0	0.031785170019651046	4.469702235141648E-5
42-43	0.0	0.001028031514082579	0.0	0.03976917563717282	5.028415014534354E-5
44-45	0.0	0.001106251303197558	0.0	0.04983159279403545	5.5871277939270605E-5
46-47	0.0	0.0011732968367246827	0.0	0.059676111966934925	5.5871277939270605E-5
48-49	0.0	0.0011788839645186099	0.0	0.06954297965101013	5.5871277939270605E-5
50-51	0.0	0.0013911948206878381	0.0	0.07837622869320879	5.5871277939270605E-5
52-53	0.0	0.0014079562040696192	0.0	0.08649432537778481	5.5871277939270605E-5
54-55	0.0	0.0014079562040696192	0.0	0.09475210025720901	5.5871277939270605E-5
56-57	0.0	0.0014079562040696192	0.0	0.10108231604772838	5.5871277939270605E-5
58-59	0.0	0.0014079562040696192	0.0	0.10674766363077041	6.145840573319766E-5
60-61	0.0	0.0014191304596574733	0.0	0.1118040142842744	6.704553352712472E-5
62-63	0.0	0.0014805888653906711	0.0	0.11702797877159621	6.704553352712472E-5
64-65	0.0	0.0017096611049416805	0.0	0.12208991655289413	6.704553352712472E-5
66-67	0.0	0.0017320096161173887	0.0	0.12786141956402075	6.704553352712472E-5
68-69	0.0	0.0017822937662627324	0.0	0.13329210777971787	6.704553352712472E-5
70-71	0.0	0.0018102294052323676	0.0	0.13852165939483363	6.704553352712472E-5
72-73	0.0	0.0018214036608202215	0.0	0.1427734636460121	7.821978911497885E-5
74-75	0.0	0.0018437521719959298	0.0	0.1462207214948651	7.821978911497885E-5
76-77	0.0	0.0018996234499352004	0.0	0.14912044081991324	7.821978911497885E-5
78-79	0.0	0.0019331462166987628	0.0	0.15194752748364032	7.821978911497885E-5
80-81	0.0	0.0019331462166987628	0.0	0.15323815400403748	7.821978911497885E-5
82-83	0.0	0.0019331462166987628	0.0	0.15372423412210914	7.821978911497885E-5
84-85	0.0	0.0019331462166987628	0.0	0.15403152615077514	7.821978911497885E-5
86-87	0.0	0.0019890174946380333	0.0	0.15411533306768405	7.821978911497885E-5
88-89	0.0	0.002123108561692283	0.0	0.15413768157885974	7.821978911497885E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12320	0.0	51.99576	1
GTATCAA	23440	0.0	38.58188	1
TCAACGC	28805	0.0	30.984676	4
ATCAACG	28765	0.0	30.983114	3
CAACGCA	29500	0.0	30.240185	5
TATCAAC	29970	0.0	29.946882	2
AACGCAG	30405	0.0	29.396406	6
ACGCAGA	34865	0.0	25.611404	7
CGCAGAG	35380	0.0	25.238598	8
GCAGAGT	40840	0.0	21.781994	9
GTGGTAT	5870	0.0	21.738283	1
GAGTACT	24480	0.0	19.953068	12-13
TGGTATC	5925	0.0	19.94655	2
AGAGTAC	37125	0.0	19.14155	10-11
CAGAGTA	39825	0.0	19.036987	10-11
GTACATG	26820	0.0	18.903404	1
GTACTTT	26655	0.0	18.373474	14-15
TACATGG	27175	0.0	18.231016	2
AGTACTT	26500	0.0	17.79402	12-13
ACATGGG	27670	0.0	17.450533	3
>>END_MODULE