FastQCFastQC Report
Fri 27 May 2016
SRR2138848_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138848_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7755738
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111450.051.6277281
GTATCAA205250.038.5618671
ATCAACG254500.030.655973
TCAACGC256200.030.3693964
CAACGCA262250.029.6687855
TATCAAC264800.029.6322822
AACGCAG271000.028.820916
ACGCAGA307800.025.2643857
CGCAGAG311850.024.9362778
CTTATAC60650.023.8230971
GCAGAGT358150.021.5827319
GAGTACT214750.020.32872212-13
GTGGTAT50850.020.032551
TATACAC91550.019.8273983
GTACATG238050.019.4800471
AGAGTAC328150.019.2704910-11
TACATGG236050.019.0492442
CAGAGTA350950.019.02026210-11
GTACTTT239700.018.21385214-15
TGGTATC54350.018.036722
Produced by FastQC (version 0.11.5)