##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138848_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7755738
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.96168591564078	34.0	31.0	34.0	31.0	34.0
2	33.071896575155066	34.0	33.0	34.0	31.0	34.0
3	33.13107044100768	34.0	33.0	34.0	31.0	34.0
4	36.48570271455792	37.0	37.0	37.0	35.0	37.0
5	36.45051083468781	37.0	37.0	37.0	35.0	37.0
6	36.4815160078899	37.0	37.0	37.0	35.0	37.0
7	36.44068842965041	37.0	37.0	37.0	35.0	37.0
8	36.42736629318835	37.0	37.0	37.0	35.0	37.0
9	38.22231759247153	39.0	39.0	39.0	37.0	39.0
10-11	38.18042267544365	39.0	39.0	39.0	37.0	39.0
12-13	38.15104384650436	39.0	39.0	39.0	37.0	39.0
14-15	39.622840985603176	41.0	40.0	41.0	37.0	41.0
16-17	39.602140698925105	41.0	40.0	41.0	37.0	41.0
18-19	39.61553858575419	41.0	40.0	41.0	37.0	41.0
20-21	39.59646983852738	41.0	40.0	41.0	37.0	41.0
22-23	39.52137534269839	41.0	39.5	41.0	37.0	41.0
24-25	39.36760743843601	41.0	39.0	41.0	36.0	41.0
26-27	39.37267285296112	41.0	39.0	41.0	36.5	41.0
28-29	39.293663985811406	41.0	39.0	41.0	36.0	41.0
30-31	39.201648498705225	41.0	39.0	41.0	36.0	41.0
32-33	39.00290270644052	40.5	39.0	41.0	35.0	41.0
34-35	38.9246393938947	40.0	38.5	41.0	35.0	41.0
36-37	39.11115057629383	41.0	39.0	41.0	35.0	41.0
38-39	39.177315707660085	41.0	39.0	41.0	35.0	41.0
40-41	39.15914663349855	41.0	39.0	41.0	35.0	41.0
42-43	39.01497793902538	41.0	39.0	41.0	35.0	41.0
44-45	39.036906548031745	41.0	39.0	41.0	35.0	41.0
46-47	38.98856256546517	40.0	39.0	41.0	35.0	41.0
48-49	38.922112591843735	40.0	39.0	41.0	35.0	41.0
50-51	38.84900996525304	40.0	38.0	41.0	35.0	41.0
52-53	38.73440516594413	40.0	38.0	41.0	35.0	41.0
54-55	38.60348538930117	40.0	38.0	41.0	35.0	41.0
56-57	38.45515524979748	40.0	37.5	41.0	34.0	41.0
58-59	38.293581283630715	40.0	37.0	41.0	34.0	41.0
60-61	38.11278208552632	40.0	37.0	41.0	34.0	41.0
62-63	37.888379471857945	39.0	36.0	41.0	34.0	41.0
64-65	37.62472102374507	39.0	36.0	41.0	33.5	41.0
66-67	37.31229922858212	39.0	35.0	40.5	33.0	41.0
68-69	36.989539479805565	38.0	35.0	40.0	33.0	41.0
70-71	36.64068005228599	37.0	35.0	40.0	33.0	41.0
72-73	36.30459099861754	37.0	35.0	39.0	32.0	41.0
74-75	35.965037420528205	36.0	35.0	39.0	32.0	40.5
76-77	35.10589875370604	35.0	34.0	38.0	31.0	39.0
78-79	35.316366158576855	35.0	34.5	37.0	32.0	39.0
80-81	35.09668227995353	35.0	34.5	37.0	32.0	39.0
82-83	34.825563651787824	35.0	34.0	36.5	31.5	39.0
84-85	34.564366097429435	35.0	34.0	36.0	31.0	37.0
86-87	34.3467483810306	35.0	34.0	36.0	31.0	37.0
88-89	34.148998085345845	35.0	34.0	35.5	31.0	37.0
90-91	33.95300955162597	35.0	34.0	35.0	31.0	36.0
92-93	33.82133134834406	35.0	34.0	35.0	31.0	36.0
94-95	33.74481318758102	35.0	34.0	35.0	31.0	36.0
96-97	33.71768439849456	35.0	34.0	35.0	31.0	35.5
98-99	33.736288156522136	35.0	34.0	35.0	31.0	35.0
100-101	32.33972545150272	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	2.0
20	31.0
21	95.0
22	566.0
23	1897.0
24	5162.0
25	10849.0
26	19186.0
27	31209.0
28	45531.0
29	62499.0
30	81788.0
31	105994.0
32	136592.0
33	182555.0
34	267038.0
35	403292.0
36	732075.0
37	1654535.0
38	2537714.0
39	1392180.0
40	84946.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.14934701232739	21.208277331770965	15.532813391275823	24.10956226462582
2	13.55369455624814	21.62161709005612	41.64035462539697	23.18433372829877
3	16.69450842576385	26.75195494018878	31.402590289303294	25.150946344744067
4	11.752050353441632	19.049616954470856	39.27578246603546	29.922550226052046
5	12.909475914410198	37.98099394035667	35.79650779803389	13.31302234719924
6	29.09941751498504	38.643269961741865	19.007493292706197	13.249819230566901
7	25.853839236879452	33.20385923498665	23.227434050729435	17.714867477404457
8	24.347324014922727	36.56202687937598	21.33523714085382	17.755411964847468
9	25.301581067572286	17.023859456305026	21.467089598127007	36.20746987799568
10-11	23.716846804262858	27.176517566735754	29.070811829899363	20.035823799102033
12-13	24.72592550186713	24.70585648973702	29.175572975776127	21.39264503261972
14-15	21.88453285101409	26.11994650305119	26.98278304692136	25.012737599013356
16-17	21.28831391071432	29.29179881620489	27.61015269476751	21.809734578313282
18-19	21.28629796416537	28.26625267640552	29.588460827325523	20.858988532103588
20-21	22.182118894261333	27.340553436670895	29.475696766490817	21.001630902576956
22-23	21.82173433061429	27.372025059514606	29.34919564209042	21.457044967780682
24-25	21.673168407600986	27.56024912205216	29.387862574271523	21.378719896075328
26-27	21.62442635441395	27.745119185562793	29.284028497766197	21.34642596225706
28-29	21.457370256182326	27.804787268451115	29.318022393735237	21.419820081631322
30-31	21.96800786254039	27.560434203087965	29.212942073407916	21.25861586096373
32-33	21.558342592865777	27.809323080846276	29.15378899547731	21.478545330810633
34-35	21.656197062312167	27.829900622264137	29.248374279679567	21.265528035744133
36-37	21.58787354398452	27.788410647381006	29.197084295847652	21.426631512786816
38-39	21.504528703244873	27.684299777243293	29.29610667098272	21.515064848529118
40-41	21.7526928256737	27.60998818642406	29.132175147649868	21.505143840252373
42-43	21.79793301862816	27.670713196263886	28.90924142434841	21.62211236075954
44-45	21.774468869383906	27.91230968705673	28.669513195695927	21.64370824786344
46-47	21.93213230763298	27.803060155115134	28.299163538410944	21.965643998840942
48-49	21.87463982132052	27.94947470907162	28.272907878249747	21.902977591358113
50-51	21.822577161376504	28.04392181837208	28.183478020244017	21.950023000007402
52-53	21.96442458607097	27.998273583605627	28.151509767447504	21.885792062875904
54-55	21.984593859082135	27.974419059312584	28.05874658139499	21.982240500210292
56-57	22.040739828771553	28.090365255469795	27.906020984607867	21.962873931150785
58-59	21.877745766524	28.27900912833097	28.037964339727022	21.805280765418008
60-61	22.047290950803223	28.277324374554325	27.880881669188103	21.794503005454352
62-63	21.953318325589922	28.325848260725767	27.82447271262597	21.89636070105834
64-65	21.93289894447753	28.289528331963982	27.825071712058048	21.95250101150044
66-67	22.036896181376104	28.228727478523524	27.721488822023392	22.01288751807698
68-69	21.995879374228693	28.13058844875234	27.835411890134644	22.038120286884325
70-71	21.995992943157123	27.937456287599293	27.899627429292412	22.166923339951175
72-73	22.09970451702227	27.93417441156866	27.855765832760277	22.1103552386488
74-75	21.941274017227595	28.04768047404143	27.826924674107325	22.18412083462365
76-77	22.092812625684736	28.00697131348151	27.74116660379182	22.15904945704193
78-79	22.05435466705255	28.01803770764881	27.726326748710832	22.20128087658781
80-81	22.02756068879204	27.989009589118375	27.803167855204496	22.18026186688509
82-83	22.0935752228646	27.937422094508197	27.854636576158896	22.114366106468307
84-85	22.24109187347862	27.867075098737114	27.803838147221544	22.08799488056272
86-87	22.03373068313623	27.923689370846873	27.861597212865284	22.180982733151613
88-89	22.112931110576636	27.858545806108424	27.832813883491212	22.195709199823728
90-91	22.17307529736863	27.830839693472655	27.87990982625616	22.116175182902555
92-93	22.166885791978594	27.782343007156	27.907399141812856	22.14337205905255
94-95	22.03594357789365	27.796154135686557	27.962382602825407	22.205519683594385
96-97	22.03150643275308	27.819969393307296	27.968308253976602	22.18021591996302
98-99	22.22406883691203	28.38484243722006	27.994379576380485	21.396709149487428
100-101	20.06468897942178	32.115159281284946	26.16300523945943	21.657146499833843
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1748.0
1	1590.0
2	2230.5
3	3222.5
4	4367.0
5	6523.0
6	8237.0
7	9985.0
8	11706.0
9	12115.5
10	11231.0
11	10445.5
12	11724.5
13	15829.0
14	25447.5
15	38464.0
16	48400.5
17	53112.5
18	52945.5
19	49339.0
20	44163.5
21	39135.5
22	36605.5
23	38689.0
24	45118.5
25	55700.0
26	70093.0
27	86438.5
28	104511.0
29	123562.5
30	141804.5
31	160052.5
32	178639.5
33	196770.0
34	214626.5
35	230194.0
36	246692.5
37	262113.5
38	272981.0
39	282119.0
40	291899.0
41	300842.5
42	305874.0
43	310558.0
44	316148.5
45	321130.0
46	324428.5
47	326047.5
48	321027.0
49	310866.0
50	301714.5
51	290217.0
52	274983.5
53	260618.0
54	246032.5
55	227022.0
56	206352.5
57	185127.5
58	162764.5
59	140645.5
60	118083.0
61	95876.5
62	75242.0
63	57299.5
64	42655.0
65	31637.0
66	23954.5
67	18265.5
68	14098.5
69	10295.5
70	7201.0
71	5162.0
72	3571.5
73	2360.0
74	1585.5
75	1015.5
76	599.5
77	381.0
78	253.0
79	169.5
80	117.0
81	83.5
82	63.0
83	42.5
84	28.0
85	22.5
86	18.5
87	14.0
88	9.5
89	6.0
90	3.5
91	2.5
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.578735898505081E-5
2	0.029874655384181364
3	2.578735898505081E-5
4	2.578735898505081E-5
5	1.2893679492525406E-5
6	2.578735898505081E-5
7	5.157471797010162E-5
8	6.70471333611321E-4
9	3.739167052832367E-4
10-11	0.0
12-13	0.0
14-15	0.0020049671610877003
16-17	5.157471797010162E-5
18-19	0.0
20-21	2.127511979181844E-4
22-23	0.0019406910626792277
24-25	7.480621964705393E-4
26-27	0.014411849766078389
28-29	0.31375018614514655
30-31	0.3144046442703712
32-33	0.33219694962780705
34-35	0.21565852548640277
36-37	0.07389909918140027
38-39	0.06501380361735123
40-41	0.026058302130119154
42-43	0.01035933510911156
44-45	0.013658087220155333
46-47	0.010060642183960404
48-49	0.007301602101764452
50-51	0.007344523796499152
52-53	0.003289897164924148
54-55	0.0013909211206525021
56-57	0.0037340791477400103
58-59	0.004117809971362633
60-61	0.0032746096290256783
62-63	0.002146689477364797
64-65	5.728559269547001E-4
66-67	0.008034392243480513
68-69	0.01159728672968284
70-71	0.012123455395301127
72-73	0.010290305889663455
74-75	0.01038061989974868
76-77	0.00913420516671795
78-79	0.002448447774648331
80-81	0.001925536633931218
82-83	0.0017854908121216488
84-85	8.129187090233083E-6
86-87	0.002778573580615421
88-89	0.0
90-91	0.008570946026806078
92-93	0.01850156784764211
94-95	0.0014916716157786943
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	118.0
20-21	452.0
22-23	987.0
24-25	2829.0
26-27	3958.0
28-29	6913.0
30-31	16241.0
32-33	21505.0
34-35	30209.0
36-37	43176.0
38-39	50756.0
40-41	53627.0
42-43	63885.0
44-45	76532.0
46-47	74817.0
48-49	65173.0
50-51	57563.0
52-53	54956.0
54-55	59581.0
56-57	62721.0
58-59	62192.0
60-61	61376.0
62-63	62042.0
64-65	66011.0
66-67	71122.0
68-69	67787.0
70-71	63814.0
72-73	61547.0
74-75	62746.0
76-77	63599.0
78-79	66572.0
80-81	67676.0
82-83	65899.0
84-85	67763.0
86-87	71023.0
88-89	75830.0
90-91	81436.0
92-93	83149.0
94-95	93564.0
96-97	146517.0
98-99	661497.0
100-101	4886577.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.7965655931988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.95869074394119	75.43391454753696
2	7.73801470668693	13.123141432734304
3	1.9310411074305782	4.9123696178820095
4	0.6767134122724359	2.295318930062279
5	0.3091648435411617	1.310805846722458
6	0.14125658249562165	0.7186843837836646
7	0.08286811923655471	0.49188523358992714
8	0.04499602539921561	0.30524067353582607
9	0.0341936370204475	0.26095526860269663
>10	0.08149067760418675	0.979133044229281
>50	0.001187937134806209	0.0656085881016074
>100	3.3606068527077877E-4	0.06592479983580167
>500	2.3073276957149767E-5	0.01586749595430011
>1k	2.307327470836942E-5	0.021150137428910518
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.736207695515242E-5	0.0	1.2893679492525406E-5	0.0
3	0.0	7.736207695515242E-5	0.0	2.578735898505081E-5	0.0
4	0.0	1.5472415391030485E-4	0.0	2.578735898505081E-5	1.2893679492525406E-5
5	0.0	1.6761783340283026E-4	0.0	2.578735898505081E-5	1.2893679492525406E-5
6	0.0	1.6761783340283026E-4	0.0	2.578735898505081E-5	2.578735898505081E-5
7	0.0	1.8051151289535567E-4	0.0	2.578735898505081E-5	2.578735898505081E-5
8	0.0	2.3208623086545729E-4	0.0	2.578735898505081E-5	2.578735898505081E-5
9	0.0	3.094483078206097E-4	0.0	3.868103847757621E-5	3.868103847757621E-5
10-11	1.2893679492525406E-5	3.094483078206097E-4	0.0	5.1574717970101616E-5	3.868103847757621E-5
12-13	1.2893679492525406E-5	3.094483078206097E-4	0.0	7.736207695515242E-5	3.868103847757621E-5
14-15	1.2893679492525406E-5	4.6417246173091457E-4	0.0	9.025575644767783E-5	5.802155771636432E-5
16-17	1.2893679492525406E-5	4.7706614122344E-4	0.0	1.3538363467151677E-4	8.380891670141513E-5
18-19	1.2893679492525406E-5	5.028535002084909E-4	0.0	1.5472415391030485E-4	9.025575644767783E-5
20-21	1.2893679492525406E-5	5.41534538686067E-4	0.0	1.6117099365656754E-4	9.670259619394054E-5
22-23	1.2893679492525406E-5	5.931092566561686E-4	0.0	1.9340519238788108E-4	1.0314943594020325E-4
24-25	1.2893679492525406E-5	6.769181733575838E-4	0.0	2.449799103579827E-4	1.0314943594020325E-4
26-27	1.2893679492525406E-5	7.091523720888972E-4	0.0	3.2234198731313513E-4	1.0314943594020325E-4
28-29	1.2893679492525406E-5	7.091523720888972E-4	0.0	7.994081285365751E-4	1.0314943594020325E-4
30-31	2.578735898505081E-5	7.929612887903124E-4	0.0	0.0024111180651022507	1.0314943594020325E-4
32-33	2.578735898505081E-5	7.994081285365751E-4	0.0	0.005499154303562085	1.0314943594020325E-4
34-35	2.578735898505081E-5	9.283449234618291E-4	0.0	0.009934580048990824	1.1604311543272864E-4
36-37	2.578735898505081E-5	0.0010379411991482952	0.0	0.01693584801343212	1.1604311543272864E-4
38-39	2.578735898505081E-5	0.001083069077372134	0.0	0.025793805824797074	1.2893679492525405E-4
40-41	2.578735898505081E-5	0.0012184527120436507	0.0	0.03470978519387839	1.4183047441777946E-4
42-43	2.578735898505081E-5	0.0012571337505212269	0.0	0.043296975735900314	1.4183047441777946E-4
44-45	2.578735898505081E-5	0.0013989642249390066	0.0	0.05363770668890568	1.4183047441777946E-4
46-47	2.578735898505081E-5	0.0015214541801179978	0.0	0.06416539599455268	1.4183047441777946E-4
48-49	2.578735898505081E-5	0.001534347859610523	0.0	0.07369382513952896	1.4183047441777946E-4
50-51	2.578735898505081E-5	0.0016503909750432517	0.0	0.08202958893144663	1.4183047441777946E-4
52-53	3.223419873131351E-5	0.0016632846545357773	0.0	0.09039114008234936	1.5472415391030488E-4
54-55	3.868103847757621E-5	0.0016826251737745654	0.0	0.09897833062437128	1.6761783340283026E-4
56-57	3.868103847757621E-5	0.0017277530519984043	0.0	0.10577974655667842	1.6761783340283026E-4
58-59	3.868103847757621E-5	0.0017535404109834552	0.0	0.11108549566785263	1.6761783340283026E-4
60-61	3.868103847757621E-5	0.0018244556481923449	0.0	0.11615271170841511	1.6761783340283026E-4
62-63	3.868103847757621E-5	0.0018953708854012345	0.0	0.12095560731938082	1.8051151289535567E-4
64-65	3.868103847757621E-5	0.0021274571162666917	0.0	0.1262484627510625	1.8051151289535567E-4
66-67	3.868103847757621E-5	0.002159691314998005	0.0	0.13143816874680397	1.8051151289535567E-4
68-69	5.157471797010162E-5	0.002249947071445683	0.0	0.1364151290309188	1.8695835264161836E-4
70-71	5.157471797010162E-5	0.002301521789415785	0.0	0.1410633004879742	2.062988718804065E-4
72-73	5.157471797010162E-5	0.002320862308654573	0.0	0.14476378650232896	2.062988718804065E-4
74-75	5.157471797010162E-5	0.0023659901868784117	0.0	0.14817416472810196	2.1919255137293187E-4
76-77	5.157471797010162E-5	0.0025078206612961914	0.0	0.151242860447323	2.1919255137293187E-4
78-79	5.157471797010162E-5	0.002578735898505081	0.0	0.15356372275597757	2.1919255137293187E-4
80-81	5.157471797010162E-5	0.002578735898505081	0.0	0.15471126023081233	2.2563939111919457E-4
82-83	5.157471797010162E-5	0.002585182738251344	0.0	0.15510451745533435	2.3208623086545729E-4
84-85	5.157471797010162E-5	0.002610970097236395	0.0	0.1553559442054386	2.3208623086545729E-4
86-87	5.157471797010162E-5	0.002681885334445284	0.0	0.15539462524391617	2.3208623086545729E-4
88-89	5.157471797010162E-5	0.0027592474114004365	0.0	0.15548488100036387	2.3208623086545729E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11145	0.0	51.627728	1
GTATCAA	20525	0.0	38.561867	1
ATCAACG	25450	0.0	30.65597	3
TCAACGC	25620	0.0	30.369396	4
CAACGCA	26225	0.0	29.668785	5
TATCAAC	26480	0.0	29.632282	2
AACGCAG	27100	0.0	28.82091	6
ACGCAGA	30780	0.0	25.264385	7
CGCAGAG	31185	0.0	24.936277	8
CTTATAC	6065	0.0	23.823097	1
GCAGAGT	35815	0.0	21.582731	9
GAGTACT	21475	0.0	20.328722	12-13
GTGGTAT	5085	0.0	20.03255	1
TATACAC	9155	0.0	19.827398	3
GTACATG	23805	0.0	19.480047	1
AGAGTAC	32815	0.0	19.27049	10-11
TACATGG	23605	0.0	19.049244	2
CAGAGTA	35095	0.0	19.020262	10-11
GTACTTT	23970	0.0	18.213852	14-15
TGGTATC	5435	0.0	18.03672	2
>>END_MODULE