FastQCFastQC Report
Fri 27 May 2016
SRR2138847_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138847_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7718435
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69300.038.7399831
GTATCAA121550.032.6293751
TCAACGC133700.028.0867964
ATCAACG134700.027.8806293
CAACGCA138450.027.1835085
AACGCAG144150.026.2551616
TATCAAC149500.025.5458352
ACGCAGA174600.021.6302837
CGCAGAG175300.021.4955648
GTACATG294300.020.0718231
TACATGG289400.019.816712
ACATGGG297900.018.6420483
CATGGGG195250.017.1245084
GCAGAGT220200.016.9031949
GGACCGA11250.016.8021376
GTGGTAT47550.016.169341
AGAGTAC192650.015.64586610-11
TGGTATC45600.015.2946882
TATACCG4403.8198777E-1115.2741755
ACCGAAC8400.015.0030758
Produced by FastQC (version 0.11.5)