FastQCFastQC Report
Fri 27 May 2016
SRR2138846_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138846_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9226667
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77750.040.3946721
GTATCAA126900.032.6072851
TCAACGC137800.028.9417484
ATCAACG140950.028.2964823
CAACGCA144900.027.5231765
AACGCAG152900.026.138996
TATCAAC158300.025.5478322
ACGCAGA186050.021.4144047
CGCAGAG189100.021.0462478
GTACATG301150.020.5890881
TACATGG298050.020.1031882
ACATGGG305200.019.0716323
CATGGGG210600.016.9342214
GCAGAGT237750.016.3636959
AGAGTAC200950.015.4690110-11
CAGAGTA229750.015.02293310-11
GTATAAT60450.014.0990781
GTGGTAT50050.013.7932931
TAGCGCG2950.00688053613.69239694
GTATAAG39550.013.5762891
Produced by FastQC (version 0.11.5)