FastQCFastQC Report
Fri 27 May 2016
SRR2138845_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138845_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10265249
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143800.050.4281461
GTATCAA263550.037.0556451
ATCAACG331400.028.967783
TCAACGC332550.028.8547084
TATCAAC340050.028.3915522
CAACGCA338900.028.3140535
AACGCAG345850.027.8070836
CTTATAC74750.025.6017021
ACGCAGA400700.023.957887
CGCAGAG406150.023.6575188
TATACAC109650.021.6333683
GCAGAGT470600.020.3541479
GTACATG325050.020.3157861
TACATGG328150.019.9017662
GAGTACT271850.019.5977512-13
GTGGTAT72700.019.5951611
AGAGTAC425200.019.07205210-11
ACATGGG332250.019.068793
TGGTATC73200.018.5235982
CAGAGTA455400.018.2594110-11
Produced by FastQC (version 0.11.5)