##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138845_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10265249
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.90523210883633	34.0	31.0	34.0	31.0	34.0
2	33.0230238935266	34.0	31.0	34.0	31.0	34.0
3	33.08319807926724	34.0	33.0	34.0	31.0	34.0
4	36.45822804687933	37.0	37.0	37.0	35.0	37.0
5	36.417609743319424	37.0	37.0	37.0	35.0	37.0
6	36.44871761025963	37.0	37.0	37.0	35.0	37.0
7	36.40491331481584	37.0	37.0	37.0	35.0	37.0
8	36.38895169518051	37.0	37.0	37.0	35.0	37.0
9	38.17133115816284	39.0	39.0	39.0	37.0	39.0
10-11	38.12460175101451	39.0	39.0	39.0	37.0	39.0
12-13	38.09168082527759	39.0	38.0	39.0	37.0	39.0
14-15	39.53914181721262	41.0	40.0	41.0	37.0	41.0
16-17	39.514311830136805	41.0	39.0	41.0	37.0	41.0
18-19	39.52326363442329	41.0	39.5	41.0	37.0	41.0
20-21	39.49988143154761	41.0	39.0	41.0	37.0	41.0
22-23	39.4209339842604	41.0	39.0	41.0	36.0	41.0
24-25	39.262371924327695	40.5	39.0	41.0	36.0	41.0
26-27	39.26084161470888	40.5	39.0	41.0	36.0	41.0
28-29	39.18573372587021	41.0	39.0	41.0	36.0	41.0
30-31	39.094476276983585	40.5	39.0	41.0	35.5	41.0
32-33	38.89664827506031	40.0	38.5	41.0	35.0	41.0
34-35	38.812499426646056	40.0	38.0	41.0	35.0	41.0
36-37	39.006007052993795	40.0	39.0	41.0	35.0	41.0
38-39	39.071346488763595	41.0	39.0	41.0	35.0	41.0
40-41	39.05084404933771	41.0	39.0	41.0	35.0	41.0
42-43	38.90750781326547	40.0	39.0	41.0	35.0	41.0
44-45	38.923792733475025	40.0	38.0	41.0	35.0	41.0
46-47	38.87281034938417	40.0	38.0	41.0	35.0	41.0
48-49	38.80064088638957	40.0	38.0	41.0	35.0	41.0
50-51	38.71674336849748	40.0	38.0	41.0	35.0	41.0
52-53	38.59485086306569	40.0	38.0	41.0	35.0	41.0
54-55	38.44574833208593	40.0	38.0	41.0	34.0	41.0
56-57	38.29105101669168	40.0	37.0	41.0	34.0	41.0
58-59	38.12429017046033	40.0	37.0	41.0	34.0	41.0
60-61	37.930776512394125	39.5	36.0	41.0	34.0	41.0
62-63	37.6956481195715	39.0	36.0	41.0	33.5	41.0
64-65	37.4194132725811	39.0	35.0	40.5	33.0	41.0
66-67	37.0962939752643	38.0	35.0	40.0	33.0	41.0
68-69	36.76309837013666	37.5	35.0	40.0	32.5	41.0
70-71	36.409441581398696	37.0	35.0	39.5	32.0	41.0
72-73	36.0753138255473	36.5	35.0	39.0	32.0	41.0
74-75	35.738362014053436	36.0	35.0	39.0	32.0	40.0
76-77	34.883931396110924	35.0	34.0	37.0	30.5	39.0
78-79	35.12327398494759	35.0	34.0	37.0	31.0	39.0
80-81	34.918440960986736	35.0	34.0	37.0	31.0	39.0
82-83	34.66033783707233	35.0	34.0	36.5	31.0	38.5
84-85	34.409770521409506	35.0	34.0	36.0	31.0	37.0
86-87	34.202118259618246	35.0	34.0	36.0	31.0	37.0
88-89	34.01133780473074	35.0	34.0	35.0	31.0	36.5
90-91	33.818768725552005	35.0	34.0	35.0	31.0	36.0
92-93	33.69066450840913	35.0	34.0	35.0	30.0	36.0
94-95	33.619214199956474	35.0	34.0	35.0	30.0	36.0
96-97	33.5994900092331	35.0	34.0	35.0	30.5	35.5
98-99	33.62777755464158	35.0	34.0	35.0	31.0	35.0
100-101	32.21684742746996	33.5	31.5	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	7.0
20	30.0
21	199.0
22	860.0
23	3069.0
24	7901.0
25	16042.0
26	28714.0
27	45460.0
28	66484.0
29	91119.0
30	118481.0
31	151767.0
32	194798.0
33	259700.0
34	380157.0
35	577637.0
36	1052855.0
37	2329501.0
38	3258624.0
39	1592696.0
40	89143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.94392625059558	21.21153612542667	15.598481829325328	24.246055794652424
2	13.568295915494701	21.44083908934297	41.505400464404566	23.485464530757763
3	16.828778337476276	26.523428705918384	31.31307384750238	25.334719109102956
4	11.87495792844382	18.76754280388133	38.900391018279244	30.45710824939561
5	13.061543855390163	37.90147710981	35.53969319205019	13.497285842749651
6	29.554188115651165	38.29676221200285	18.68418389071712	13.464865781628873
7	26.10002976061458	33.10585378137589	22.874680536119694	17.919435921889836
8	24.612147835202613	36.462326502186464	20.997737303762708	17.92778835884821
9	25.337765137437717	16.94952701823979	21.260502114182923	36.45220573013957
10-11	24.089727389954206	26.960826766111566	28.682441117599776	20.267004726334452
12-13	24.98795694093733	24.475382915699363	28.886634897994195	21.650025245369108
14-15	22.1447986846225	25.879266106954503	26.709306872512308	25.26662833591069
16-17	21.575853433196524	29.090574953698127	27.24157360506969	22.09199800803566
18-19	21.573453461621188	28.034174137828916	29.22761827088834	21.164754129661553
20-21	22.494938117611024	27.045800293737255	29.173096309424025	21.286165279227696
22-23	22.188986366982785	27.059594756067934	29.014677540188828	21.736741336760453
24-25	21.96802145292213	27.311426605386803	29.05836949430957	21.662182447381497
26-27	21.906888759265218	27.442777611442203	29.001043478125226	21.64929015116735
28-29	21.662162642755085	27.425159197266076	29.101489666590368	21.81118849338847
30-31	22.102707176013723	27.20725826067696	29.04079972932696	21.649234833982355
32-33	21.873635455097947	27.521328065342377	28.826236051611886	21.778800427947782
34-35	21.86911848579057	27.56840437908366	29.00538276377081	21.557094371354953
36-37	21.98074209660078	27.43691150882653	28.821222083976846	21.761124310595847
38-39	21.825667867175884	27.459623097878637	28.917179728404175	21.797529306541303
40-41	22.057317410805844	27.383067433171508	28.786539830698754	21.77307532532389
42-43	22.104544542724376	27.456823447254227	28.55629858406456	21.88233342595684
44-45	22.107083997426884	27.636399114388993	28.293193879422336	21.963323008761783
46-47	22.209609038486526	27.579289189779328	27.93168502102075	22.279416750713395
48-49	22.12879213675824	27.730571064106226	27.943323612345587	22.19731318678995
50-51	22.08244331641814	27.849394190603867	27.846763457874612	22.221399035103385
52-53	22.25250892049274	27.776257740907763	27.788018898913684	22.183214439685813
54-55	22.243233607958803	27.726556517940555	27.69616467253367	22.33404520156698
56-57	22.329156172543588	27.893953385888672	27.555591026317792	22.221299415249952
58-59	22.16187276408466	28.070919803024974	27.716680035688857	22.050527397201513
60-61	22.344228596395272	28.043981559713423	27.548561755520474	22.06322808837084
62-63	22.250583603951515	28.134568831023238	27.474032541359378	22.14081502366587
64-65	22.20866444360411	28.122553542576235	27.499610161916877	22.16917185190278
66-67	22.31393423096263	28.013874516265684	27.402469559029374	22.26972169374231
68-69	22.29426198597264	27.906895071034988	27.51170583046908	22.287137112523293
70-71	22.260785175332202	27.70623287960644	27.57382931653684	22.459152628524517
72-73	22.345219435176702	27.692842027125415	27.579872337333907	22.382066200363976
74-75	22.177205102488834	27.811380075615844	27.552438388224843	22.458976433670482
76-77	22.318339775184246	27.78194258496493	27.449358295939817	22.450359343911007
78-79	22.340152394351893	27.77088815511044	27.446612837089273	22.442346613448397
80-81	22.33148382689399	27.7211236612674	27.48587157288254	22.461520938956074
82-83	22.3264346583485	27.687327807904655	27.598848381306546	22.387389152440303
84-85	22.477844501628617	27.62429877301953	27.530262203714166	22.367594521637688
86-87	22.29584527989073	27.594566070324188	27.628017350529877	22.4815712992552
88-89	22.347745069565548	27.605955214521398	27.570759864202383	22.47553985171067
90-91	22.445330724583854	27.591854258487835	27.589127736667745	22.373687280260558
92-93	22.427614829597378	27.51653154054733	27.60656111978374	22.449292510071555
94-95	22.264766768309038	27.56317118515645	27.69122713030447	22.48083491623004
96-97	22.26789890420699	27.59252505013712	27.718956632217385	22.420619413438512
98-99	22.469858372051085	28.104773372253693	27.722043149364755	21.703325106330464
100-101	20.30949125827599	31.867025687948953	25.807649152282213	22.015833901492844
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2140.0
1	1963.5
2	2885.5
3	4142.0
4	5477.5
5	8116.5
6	10206.5
7	12443.5
8	14505.0
9	14826.0
10	13452.5
11	12194.5
12	13729.0
13	19158.5
14	32175.0
15	49762.5
16	63605.5
17	70443.5
18	70151.5
19	64950.5
20	57582.0
21	50218.5
22	45836.5
23	47155.0
24	54463.5
25	67558.5
26	85744.5
27	105581.5
28	126475.5
29	150250.0
30	173140.0
31	195038.0
32	219198.5
33	244112.0
34	267160.0
35	288055.5
36	311730.0
37	333376.5
38	348145.5
39	358249.5
40	369193.0
41	380527.5
42	386312.5
43	392216.5
44	401963.5
45	414214.0
46	424110.5
47	429547.0
48	425968.0
49	417101.0
50	409511.5
51	395421.5
52	375962.5
53	358313.5
54	341604.0
55	319938.0
56	294485.5
57	266331.0
58	234960.5
59	203214.5
60	170275.5
61	137688.5
62	107835.0
63	82533.0
64	62172.5
65	46797.5
66	35635.0
67	27126.5
68	20728.5
69	15000.5
70	10471.5
71	7525.0
72	5381.5
73	3688.5
74	2465.5
75	1629.0
76	1041.5
77	662.0
78	421.5
79	268.5
80	172.0
81	107.0
82	72.0
83	50.5
84	32.0
85	25.5
86	25.0
87	20.0
88	12.0
89	6.0
90	2.5
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.029965176684949385
3	0.0
4	0.0
5	0.0
6	0.0
7	3.89664196163191E-5
8	6.03979504052946E-4
9	5.260466648203078E-4
10-11	0.0
12-13	0.0
14-15	0.0020554786347608323
16-17	4.870802452039887E-5
18-19	9.741604904079775E-6
20-21	2.3380464452926356E-4
22-23	0.0021190087949094685
24-25	8.087948941460448E-4
26-27	0.014626832746773458
28-29	0.3085242854854256
30-31	0.3088441407220485
32-33	0.3269849379416899
34-35	0.21226248379432763
36-37	0.06966495176763365
38-39	0.06158049845244088
40-41	0.024256191220903098
42-43	0.009321691888081098
44-45	0.012431864452300474
46-47	0.008911046146087507
48-49	0.006301930329736386
50-51	0.006732350843337415
52-53	0.0027124285834048485
54-55	0.00123949933088134
56-57	0.003291157775604822
58-59	0.0038711472032141032
60-61	0.0029415344611846955
62-63	0.00205563082266291
64-65	8.250473370909656E-4
66-67	0.007714381495437019
68-69	0.011370453816857161
70-71	0.011542065220937103
72-73	0.009973812027537094
74-75	0.010536979347347458
76-77	0.009599125612213488
78-79	0.0025215814159567198
80-81	0.00198335619735273
82-83	0.0016861512140408752
84-85	1.2126708092309958E-5
86-87	0.0027400242887522185
88-89	6.201823460133749E-6
90-91	0.008417584560290509
92-93	0.017839656454455854
94-95	0.0014866705121877248
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	163.0
20-21	576.0
22-23	1310.0
24-25	3621.0
26-27	5204.0
28-29	8349.0
30-31	19252.0
32-33	25543.0
34-35	36380.0
36-37	51791.0
38-39	62700.0
40-41	65937.0
42-43	79434.0
44-45	95169.0
46-47	93509.0
48-49	80493.0
50-51	70332.0
52-53	67956.0
54-55	73655.0
56-57	78696.0
58-59	78634.0
60-61	77789.0
62-63	77837.0
64-65	84871.0
66-67	90630.0
68-69	86188.0
70-71	81821.0
72-73	78175.0
74-75	79875.0
76-77	81292.0
78-79	85940.0
80-81	87922.0
82-83	86279.0
84-85	88355.0
86-87	92981.0
88-89	100544.0
90-91	108462.0
92-93	111468.0
94-95	126554.0
96-97	201752.0
98-99	901083.0
100-101	6536727.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.26454626856544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.49251360739233	71.10039420201024
2	8.253623874498556	13.414539984650489
3	2.2468087156551455	5.477576724899209
4	0.9042218528157224	2.9392471438076497
5	0.4027479980713969	1.6364566661922568
6	0.22860807193604485	1.1146638743528554
7	0.14112274559519167	0.8027793122277211
8	0.08744273853071274	0.5684795576943341
9	0.05747229423821447	0.42034139228537876
>10	0.18180381661273584	2.2241230636415272
>50	0.0029727213998498947	0.16239879833539334
>100	5.730090513145002E-4	0.07878413071292338
>500	6.641571306287866E-5	0.037942608943348895
>1k	2.2138489857406632E-5	0.022272540246667347
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.9483209808159547E-5	0.0	0.0	0.0
3	0.0	1.9483209808159547E-5	0.0	0.0	0.0
4	0.0	4.870802452039887E-5	0.0	0.0	0.0
5	0.0	4.870802452039887E-5	0.0	0.0	0.0
6	0.0	4.870802452039887E-5	0.0	0.0	9.741604904079774E-6
7	0.0	4.870802452039887E-5	0.0	0.0	9.741604904079774E-6
8	0.0	5.8449629424478645E-5	0.0	0.0	9.741604904079774E-6
9	0.0	1.5586567846527638E-4	0.0	0.0	9.741604904079774E-6
10-11	0.0	1.6073648091731625E-4	0.0	0.0	9.741604904079774E-6
12-13	0.0	1.7047808582139605E-4	0.0	0.0	9.741604904079774E-6
14-15	0.0	2.0457370298567525E-4	0.0	0.0	1.9483209808159547E-5
16-17	0.0	2.3379851769791458E-4	0.0	0.0	4.383722206835898E-5
18-19	0.0	2.7276493731423365E-4	0.0	4.870802452039887E-6	4.870802452039887E-5
20-21	0.0	3.409561716427921E-4	0.0	9.741604904079774E-6	5.357882697243876E-5
22-23	0.0	3.5556857899891176E-4	0.0	9.741604904079774E-6	6.819123432855841E-5
24-25	0.0	4.188890108754303E-4	0.0	4.870802452039887E-5	8.767444413671796E-5
26-27	0.0	4.481138255876696E-4	0.0	1.0715765394487751E-4	9.741604904079774E-5
28-29	0.0	4.578554304917494E-4	0.0	3.2634376428667246E-4	9.741604904079774E-5
30-31	0.0	5.16305059916228E-4	0.0	0.0011105429590650942	1.0228685149283763E-4
32-33	0.0	5.16305059916228E-4	0.0	0.0026448457314576586	1.0715765394487751E-4
34-35	0.0	5.747546893407067E-4	0.0	0.005167921401614321	1.120284563969174E-4
36-37	0.0	6.429459236692651E-4	0.0	0.008791798425931996	1.1689925884895729E-4
38-39	0.0	7.208787629019032E-4	0.0	0.013954849025094276	1.3151166620507695E-4
40-41	0.0	8.231656143947409E-4	0.0	0.019546530240036067	1.3638246865711685E-4
42-43	0.0	8.377780217508606E-4	0.0	0.02491415454218402	1.3638246865711685E-4
44-45	0.0	9.546772805998178E-4	0.0	0.03132900137152055	1.3638246865711685E-4
46-47	0.0	0.001076447341900815	0.0	0.03857675542015591	1.4125327110915673E-4
48-49	0.0	0.0010959305517089747	0.0	0.045736835024654546	1.5586567846527638E-4
50-51	0.0	0.0011836049958456926	0.0	0.05222961469322371	1.5586567846527638E-4
52-53	0.0	0.001207959008105892	0.0	0.058605495102943925	1.802196907254758E-4
54-55	0.0	0.0012225714154620116	0.0	0.06532720248675897	2.1431530788975503E-4
56-57	0.0	0.0012371838228181313	0.0	0.0715082507983976	2.289277152458747E-4
58-59	0.0	0.001256667032626291	0.0	0.07697329114958634	2.3379851769791458E-4
60-61	0.0	0.0012712794399824105	0.0	0.08230681983457003	2.3379851769791458E-4
62-63	0.0	0.00129563345224261	0.0	0.08769879814897817	2.4354012260199436E-4
64-65	0.0	0.0013735662914752481	0.0	0.09298361880944145	2.4354012260199436E-4
66-67	0.0	0.0013979203037354476	0.0	0.09898444743035459	2.4354012260199436E-4
68-69	0.0	0.0014320159208997267	0.0	0.10462483666981678	2.4841092505403426E-4
70-71	0.0	0.0014417575258038066	0.0	0.10997297776215657	2.5328172750607416E-4
72-73	0.0	0.0014466283282558465	0.0	0.11399138978508948	2.922481471223932E-4
74-75	0.0	0.0015099487601323651	0.0	0.11736685588435312	2.922481471223932E-4
76-77	0.0	0.001544044377296644	0.0	0.12045981544139844	2.922481471223932E-4
78-79	0.0	0.0015586567846527638	0.0	0.12334333049300605	2.922481471223932E-4
80-81	0.0	0.0015586567846527638	0.0	0.12451232308149564	3.1173135693055276E-4
82-83	0.0	0.001563527587104804	0.0	0.12496530770953534	3.409561716427921E-4
84-85	0.0	0.0016024940067211229	0.0	0.1253160054860822	3.4582697409483196E-4
86-87	0.0	0.0016317188214333622	0.0	0.12542803394247914	3.5069777654687186E-4
88-89	0.0	0.0016658144385976413	0.0	0.12545238795473934	3.6531018390299156E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14380	0.0	50.428146	1
GTATCAA	26355	0.0	37.055645	1
ATCAACG	33140	0.0	28.96778	3
TCAACGC	33255	0.0	28.854708	4
TATCAAC	34005	0.0	28.391552	2
CAACGCA	33890	0.0	28.314053	5
AACGCAG	34585	0.0	27.807083	6
CTTATAC	7475	0.0	25.601702	1
ACGCAGA	40070	0.0	23.95788	7
CGCAGAG	40615	0.0	23.657518	8
TATACAC	10965	0.0	21.633368	3
GCAGAGT	47060	0.0	20.354147	9
GTACATG	32505	0.0	20.315786	1
TACATGG	32815	0.0	19.901766	2
GAGTACT	27185	0.0	19.59775	12-13
GTGGTAT	7270	0.0	19.595161	1
AGAGTAC	42520	0.0	19.072052	10-11
ACATGGG	33225	0.0	19.06879	3
TGGTATC	7320	0.0	18.523598	2
CAGAGTA	45540	0.0	18.25941	10-11
>>END_MODULE