FastQCFastQC Report
Fri 27 May 2016
SRR2138844_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138844_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10927282
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148550.052.6209681
GTATCAA271550.038.2546961
TCAACGC338750.030.1239454
ATCAACG342050.029.858453
TATCAAC350700.029.3634682
CAACGCA351300.029.1211975
AACGCAG362500.028.2214536
ACGCAGA418200.024.472927
CGCAGAG432500.023.6538188
GTGGTAT70600.021.5593741
GCAGAGT492750.020.658059
GTACATG355600.020.6399861
TGGTATC71950.020.0194262
TACATGG359000.019.8935742
GAGTACT276900.019.8708912-13
ACATGGG362150.019.0856953
AGAGTAC445350.018.82672510-11
CAGAGTA482050.018.49914410-11
GTACTTT298350.018.36327214-15
AGTACTT301950.017.35397512-13