##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138844_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10927282
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.93395777650838	34.0	31.0	34.0	31.0	34.0
2	33.04460102704405	34.0	33.0	34.0	31.0	34.0
3	33.10442917095029	34.0	33.0	34.0	31.0	34.0
4	36.4727520530723	37.0	37.0	37.0	35.0	37.0
5	36.432281421857695	37.0	37.0	37.0	35.0	37.0
6	36.46269182034471	37.0	37.0	37.0	35.0	37.0
7	36.4194426390753	37.0	37.0	37.0	35.0	37.0
8	36.40339683738372	37.0	37.0	37.0	35.0	37.0
9	38.191156135624574	39.0	39.0	39.0	37.0	39.0
10-11	38.14411012729423	39.0	39.0	39.0	37.0	39.0
12-13	38.11691910211523	39.0	39.0	39.0	37.0	39.0
14-15	39.579249990985865	41.0	40.0	41.0	37.0	41.0
16-17	39.55595879194844	41.0	40.0	41.0	37.0	41.0
18-19	39.56307250055412	41.0	40.0	41.0	37.0	41.0
20-21	39.53807614656593	41.0	39.5	41.0	37.0	41.0
22-23	39.45835178456274	41.0	39.0	41.0	36.5	41.0
24-25	39.30131186695364	41.0	39.0	41.0	36.0	41.0
26-27	39.30483316779086	40.5	39.0	41.0	36.0	41.0
28-29	39.23108295660449	41.0	39.0	41.0	36.0	41.0
30-31	39.14475463899363	41.0	39.0	41.0	36.0	41.0
32-33	38.94820150105637	40.0	39.0	41.0	35.0	41.0
34-35	38.86697592827008	40.0	38.0	41.0	35.0	41.0
36-37	39.06147419478168	40.5	39.0	41.0	35.0	41.0
38-39	39.133807775878495	41.0	39.0	41.0	35.0	41.0
40-41	39.10918896244177	41.0	39.0	41.0	35.0	41.0
42-43	38.96026434237874	40.5	39.0	41.0	35.0	41.0
44-45	38.978121793581366	40.0	39.0	41.0	35.0	41.0
46-47	38.9250783074784	40.0	38.0	41.0	35.0	41.0
48-49	38.851572909025776	40.0	38.0	41.0	35.0	41.0
50-51	38.77084376686831	40.0	38.0	41.0	35.0	41.0
52-53	38.64666119022786	40.0	38.0	41.0	35.0	41.0
54-55	38.49822238406263	40.0	38.0	41.0	34.5	41.0
56-57	38.34078486546372	40.0	37.0	41.0	34.0	41.0
58-59	38.16915414380992	40.0	37.0	41.0	34.0	41.0
60-61	37.974983607695556	39.5	36.5	41.0	34.0	41.0
62-63	37.7310248714345	39.0	36.0	41.0	34.0	41.0
64-65	37.44509799294885	39.0	35.5	40.5	33.0	41.0
66-67	37.11280814060096	38.0	35.0	40.0	33.0	41.0
68-69	36.77113752776093	37.5	35.0	40.0	33.0	41.0
70-71	36.407168331930954	37.0	35.0	39.5	32.0	41.0
72-73	36.06562386909432	36.5	35.0	39.0	32.0	41.0
74-75	35.725351119193164	36.0	35.0	39.0	32.0	40.0
76-77	34.8797637906484	35.0	34.0	37.0	30.5	39.0
78-79	35.10726377551403	35.0	34.0	37.0	31.0	39.0
80-81	34.90234463348153	35.0	34.0	37.0	31.0	39.0
82-83	34.65124487943149	35.0	34.0	36.0	31.0	38.5
84-85	34.40668432780251	35.0	34.0	36.0	31.0	37.0
86-87	34.20580872053951	35.0	34.0	35.5	31.0	37.0
88-89	34.02055469518581	35.0	34.0	35.0	31.0	36.5
90-91	33.837713082614826	35.0	34.0	35.0	31.0	36.0
92-93	33.71467172804981	35.0	34.0	35.0	31.0	36.0
94-95	33.646804927996385	35.0	34.0	35.0	31.0	36.0
96-97	33.62693687405742	35.0	34.0	35.0	31.0	35.0
98-99	33.65460664762128	35.0	34.0	35.0	31.0	35.0
100-101	32.25801610703092	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	7.0
20	27.0
21	174.0
22	846.0
23	2891.0
24	7741.0
25	16186.0
26	29138.0
27	46974.0
28	68006.0
29	93111.0
30	122606.0
31	156502.0
32	200913.0
33	268977.0
34	392477.0
35	607098.0
36	1118786.0
37	2488227.0
38	3502006.0
39	1706227.0
40	98360.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.3107911006598	20.935608690248866	15.53507999519002	24.218520213901314
2	13.813114414944694	21.345439998681805	41.07801988463943	23.76342570173407
3	16.909191141950945	26.315995139504956	31.076721548871898	25.698092169672204
4	12.08868774504035	18.567554127366716	38.63315690031611	30.71060122727683
5	13.332089352137155	37.71117099384824	35.34049912869458	13.61624052532002
6	29.994714147580343	37.91110177260914	18.616312821431716	13.477871258378798
7	26.61407349956874	32.840447412552535	22.804697419988056	17.74078166789067
8	24.900802566220545	36.23270119201647	20.83272399749689	18.033772244266096
9	25.60678488820355	16.918064741963306	21.13911812010826	36.33603224972489
10-11	24.24421278777284	26.85369518238845	28.573084322341092	20.32900770749762
12-13	25.11016005627017	24.40783078536822	28.745931513435817	21.736077644925793
14-15	22.327692708461313	25.733093397090595	26.481409311069175	25.457804583378916
16-17	21.758316960699915	28.924886058093698	27.107397916718334	22.209399064488057
18-19	21.820741882565127	27.955565711583176	28.986078148253153	21.23761425759855
20-21	22.698717760112178	27.026918567807854	28.928280161506194	21.346083510573774
22-23	22.444744450534362	26.92057426480114	28.74942414130528	21.88525714335922
24-25	22.21013517014214	27.130559568469053	28.75399997107407	21.90530529031474
26-27	22.08159632387925	27.24165623053026	28.780224559739732	21.89652288585076
28-29	22.03029938785885	27.249548879946744	28.800137366929235	21.92001436526517
30-31	22.26114742700911	27.142660341467824	28.69363730594734	21.902554925575725
32-33	22.14381645001356	27.318669629134824	28.562745849003825	21.974768071847798
34-35	22.22277209188439	27.323613904892685	28.631743059489473	21.821870943733455
36-37	22.24259101397524	27.20724798248074	28.542179781583123	22.0079812219609
38-39	22.10780832619652	27.350901062352495	28.601988333216095	21.939302278234894
40-41	22.303475775632265	27.253629634800696	28.447314949649936	21.995579639917096
42-43	22.260202794875944	27.345984052492405	28.246737298004003	22.147075854627644
44-45	22.29688163495755	27.475214808476046	28.055330793678472	22.172572762887928
46-47	22.4227552356567	27.445869609439445	27.75727418708386	22.37410096781999
48-49	22.345547393941892	27.569080155828374	27.68316336034443	22.402209089885304
50-51	22.337816603907903	27.681219830520114	27.59832694036981	22.38263662520217
52-53	22.56341019533757	27.56174005129244	27.46637625126907	22.40847350210092
54-55	22.538951115778044	27.520129618290053	27.480925589258188	22.45999367667372
56-57	22.50772229067302	27.64113615526656	27.436738278426304	22.41440327563412
58-59	22.43075306424546	27.68513984222954	27.478425139951867	22.40568195357313
60-61	22.525560168327207	27.673907178487084	27.438316622451293	22.362216030734412
62-63	22.526462270833576	27.75439749921651	27.357578430067786	22.361561799882125
64-65	22.508520110697287	27.68628122275711	27.336108769677363	22.469089896868248
66-67	22.553307591037573	27.699028127630175	27.226107301257347	22.521556980074905
68-69	22.489465783479073	27.64481267255225	27.338575072695015	22.52714647127366
70-71	22.468600314357065	27.550119992185568	27.362260073898064	22.6190196195593
72-73	22.530806128985084	27.56073675586597	27.273675757635363	22.634781357513585
74-75	22.411789743519776	27.68663373894317	27.260535529655737	22.64104098788132
76-77	22.529882630046956	27.598453425764248	27.236735301408643	22.634928642780157
78-79	22.48207884270866	27.600123260577398	27.18128122546813	22.736516671245813
80-81	22.484833640324744	27.639387177299717	27.191130147525676	22.684649034849862
82-83	22.53229612351228	27.598853584236455	27.27142073123326	22.597429561018007
84-85	22.553695959791785	27.6195153525738	27.221812169000607	22.604976518633805
86-87	22.463676563487052	27.622926336894725	27.279135424391406	22.63426167522682
88-89	22.51525747771196	27.612658252751903	27.26707862483357	22.605005644702572
90-91	22.524260742704204	27.650873068056615	27.266464429371677	22.558401759867504
92-93	22.571791892762125	27.62536779925839	27.245619423658745	22.557220884320735
94-95	22.371910690790315	27.628128423434955	27.3488408198462	22.65112006592853
96-97	22.444165152417415	27.685003497954042	27.307144858160644	22.5636864914679
98-99	22.616935268987906	28.187962313724746	27.366416063306147	21.8286863539812
100-101	20.476816182959627	31.97402676193858	25.414313179941438	22.134843875160353
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4171.0
1	3386.0
2	3485.5
3	4536.5
4	5674.5
5	8180.0
6	10386.5
7	12782.5
8	14896.5
9	15215.5
10	14014.5
11	12860.5
12	14362.0
13	19546.0
14	31852.5
15	48633.5
16	62162.5
17	69288.5
18	69256.5
19	63941.5
20	56468.0
21	49049.5
22	44789.5
23	46531.5
24	53575.5
25	66059.5
26	82688.0
27	101350.0
28	123911.5
29	148476.5
30	170699.0
31	194077.0
32	219868.0
33	246118.0
34	272040.0
35	295125.0
36	320265.5
37	342973.5
38	361192.5
39	380358.5
40	395801.0
41	407927.5
42	416912.5
43	426057.0
44	435434.5
45	442100.0
46	446865.5
47	447085.5
48	445025.5
49	442911.5
50	437528.5
51	428181.0
52	412719.0
53	391641.0
54	370511.0
55	348050.5
56	321664.0
57	291438.0
58	260018.5
59	226784.0
60	190788.5
61	156768.0
62	126098.5
63	97704.0
64	73116.0
65	54582.0
66	42050.0
67	32954.5
68	25422.5
69	18962.5
70	13795.0
71	10012.0
72	7189.5
73	5015.5
74	3400.0
75	2233.5
76	1450.5
77	925.0
78	567.5
79	366.5
80	256.5
81	168.5
82	115.5
83	78.0
84	51.0
85	33.0
86	26.5
87	24.0
88	18.0
89	10.0
90	6.0
91	6.0
92	6.0
93	4.5
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.02994340221108964
3	0.0
4	0.0
5	0.0
6	0.0
7	6.405984580612085E-5
8	7.687181496734504E-4
9	5.216301729926984E-4
10-11	0.0
12-13	0.0
14-15	0.001944673890542955
16-17	2.7454219631194655E-5
18-19	0.0
20-21	1.830323267577292E-4
22-23	0.0018990906513304342
24-25	6.911052782639288E-4
26-27	0.014366081544131269
28-29	0.3128950780735308
30-31	0.3138017566332273
32-33	0.33076719769599544
34-35	0.21425534394590803
36-37	0.07231364876303056
38-39	0.06368827948749207
40-41	0.025559068368215802
42-43	0.009757541574214509
44-45	0.012850026091821272
46-47	0.009302237264781662
48-49	0.006252334644500817
50-51	0.006863768536748456
52-53	0.0026975120536778365
54-55	0.0010195616261156652
56-57	0.003382238439229656
58-59	0.003872418787708621
60-61	0.002869696075355988
62-63	0.0021158329620404232
64-65	6.853348796137143E-4
66-67	0.008111191541316695
68-69	0.012081134421671058
70-71	0.012388616984905114
72-73	0.010197116460689902
74-75	0.010487554265679244
76-77	0.009484915229998583
78-79	0.0023623104033018726
80-81	0.0018644428007260228
82-83	0.0016255068708774132
84-85	1.6985939096235932E-5
86-87	0.0027178896359429747
88-89	0.0
90-91	0.008733666915282318
92-93	0.019066282658797565
94-95	0.001474283678656705
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	145.0
20-21	577.0
22-23	1388.0
24-25	2719.0
26-27	4693.0
28-29	8719.0
30-31	19631.0
32-33	26596.0
34-35	38203.0
36-37	54720.0
38-39	64465.0
40-41	68676.0
42-43	83257.0
44-45	100401.0
46-47	98738.0
48-49	85793.0
50-51	74677.0
52-53	72254.0
54-55	78406.0
56-57	80953.0
58-59	80683.0
60-61	78926.0
62-63	78765.0
64-65	84936.0
66-67	89719.0
68-69	87455.0
70-71	85458.0
72-73	82561.0
74-75	84301.0
76-77	85668.0
78-79	90328.0
80-81	90845.0
82-83	89255.0
84-85	91315.0
86-87	95846.0
88-89	103245.0
90-91	109419.0
92-93	113496.0
94-95	132330.0
96-97	215623.0
98-99	968441.0
100-101	7023656.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.0983850558822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.94225842501666	74.79900296766834
2	7.636556125190904	12.84444075034321
3	1.9292489096066088	4.867401530062124
4	0.7004902632764104	2.3564039955566343
5	0.31475231671080156	1.3235080763987987
6	0.15424659542072885	0.7783133745150799
7	0.09894545433088715	0.5824807042493708
8	0.05810672225072547	0.3909345201741367
9	0.04241618323864999	0.3210419259544359
>10	0.12019971766448655	1.5055597816629214
>50	0.0021914886279874126	0.12017443568309968
>100	5.514521006072933E-4	0.08419063264910573
>500	1.8173389295029413E-5	0.011172258169045008
>1k	1.8173175171635396E-5	0.01537504691371825
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.151406543731552E-6	0.0
2	0.0	3.660562617492621E-5	0.0	9.151406543731552E-6	0.0
3	0.0	3.660562617492621E-5	0.0	9.151406543731552E-6	0.0
4	0.0	5.490843926238931E-5	0.0	9.151406543731552E-6	0.0
5	0.0	7.321125234985241E-5	0.0	9.151406543731552E-6	0.0
6	6.405984580612087E-5	7.321125234985241E-5	0.0	9.151406543731552E-6	0.0
7	6.405984580612087E-5	7.321125234985241E-5	0.0	1.8302813087463103E-5	0.0
8	6.405984580612087E-5	7.321125234985241E-5	0.0	1.8302813087463103E-5	0.0
9	6.405984580612087E-5	2.0133094396209414E-4	0.0	2.7454219631194655E-5	9.151406543731552E-6
10-11	7.321125234985241E-5	2.0133094396209414E-4	0.0	3.660562617492621E-5	1.3727109815597328E-5
12-13	7.321125234985241E-5	2.104823505058257E-4	0.0	3.660562617492621E-5	1.8302813087463103E-5
14-15	8.236265889358396E-5	2.65390789768215E-4	0.0	3.660562617492621E-5	4.575703271865776E-5
16-17	1.0524117525291285E-4	2.65390789768215E-4	0.0	4.118132944679199E-5	4.575703271865776E-5
18-19	1.0981687852477862E-4	2.7911789958381234E-4	0.0	4.575703271865776E-5	4.575703271865776E-5
20-21	1.0981687852477862E-4	3.019964159431412E-4	0.0	5.9484142534255085E-5	5.0332735990523536E-5
22-23	1.0981687852477862E-4	3.2945063557433585E-4	0.0	6.405984580612087E-5	5.9484142534255085E-5
24-25	1.326953948841075E-4	3.614805584773963E-4	0.0	9.151406543731552E-5	6.405984580612087E-5
26-27	1.4642250469970483E-4	3.752076682929936E-4	0.0	1.2811969161224173E-4	6.405984580612087E-5
28-29	1.4642250469970483E-4	3.752076682929936E-4	0.0	4.529946239147118E-4	6.405984580612087E-5
30-31	1.555739112434364E-4	4.392675140991145E-4	0.0	0.001285772619394283	6.405984580612087E-5
32-33	1.555739112434364E-4	4.392675140991145E-4	0.0	0.002923874390722231	7.321125234985241E-5
34-35	1.6014961451530214E-4	4.89600250089638E-4	0.0	0.00542678408043281	1.0981687852477862E-4
36-37	1.6472531778716792E-4	5.490843926238931E-4	0.0	0.009412221630227901	1.1439258179664439E-4
38-39	1.6472531778716792E-4	5.811143155269535E-4	0.0	0.015141002126603853	1.1896828506851017E-4
40-41	1.6472531778716792E-4	6.314470515174771E-4	0.0	0.0210573864571263	1.1896828506851017E-4
42-43	1.6472531778716792E-4	6.451741613330744E-4	0.0	0.02674498562405546	1.2354398834037595E-4
44-45	1.6472531778716792E-4	7.321125234985241E-4	0.0	0.03356735920240733	1.326953948841075E-4
46-47	1.6472531778716792E-4	8.053237758483766E-4	0.0	0.04074206193269287	1.4642250469970483E-4
48-49	1.6472531778716792E-4	8.053237758483766E-4	0.0	0.04790303755316281	1.555739112434364E-4
50-51	1.7387672433089949E-4	9.197163576450209E-4	0.0	0.05517383005215752	1.555739112434364E-4
52-53	1.8302813087463105E-4	9.334434674606183E-4	0.0	0.062138050431937236	1.8302813087463105E-4
54-55	1.8302813087463105E-4	9.425948740043498E-4	0.0	0.06912972503134815	2.1505805377769146E-4
56-57	1.8302813087463105E-4	9.517462805480814E-4	0.0	0.07654694003504256	2.1963375704955724E-4
58-59	1.8302813087463105E-4	9.654733903636788E-4	0.0	0.08233062897068091	2.1963375704955724E-4
60-61	1.9217953741836258E-4	0.001002079016538605	0.0	0.08793586547871649	2.287851635932888E-4
62-63	2.1963375704955724E-4	0.0010158061263542022	0.0	0.0935731319096551	2.287851635932888E-4
64-65	2.1963375704955724E-4	0.0011530772245101756	0.0	0.09888094770501943	2.287851635932888E-4
66-67	2.287851635932888E-4	0.0011530772245101756	0.0	0.10456854687194858	2.287851635932888E-4
68-69	2.287851635932888E-4	0.0011759557408695044	0.0	0.11042087135666491	2.287851635932888E-4
70-71	2.3793657013702034E-4	0.0011805314441413702	0.0	0.11604898638105982	2.3793657013702034E-4
72-73	2.5623938322448346E-4	0.001185107147413236	0.0	0.12012593799629222	2.7454219631194653E-4
74-75	2.5623938322448346E-4	0.001221712773588162	0.0	0.12327402184733588	2.7454219631194653E-4
76-77	2.699664930400808E-4	0.00126746980630682	0.0	0.12607435224971772	2.7454219631194653E-4
78-79	2.7454219631194653E-4	0.0012994997292098804	0.0	0.12902110515679927	2.7454219631194653E-4
80-81	2.7454219631194653E-4	0.0012994997292098804	0.0	0.1302473936336593	2.9284500939940966E-4
82-83	2.7454219631194653E-4	0.0012994997292098804	0.0	0.1305814199725055	3.2029922903060434E-4
84-85	2.7454219631194653E-4	0.0013178025422973434	0.0	0.13083765935573	3.2029922903060434E-4
86-87	2.836936028556781E-4	0.001345256761928538	0.0	0.13089714349826426	3.2029922903060434E-4
88-89	2.9284500939940966E-4	0.0013955894979190617	0.0	0.13093832482771103	3.2029922903060434E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14855	0.0	52.620968	1
GTATCAA	27155	0.0	38.254696	1
TCAACGC	33875	0.0	30.123945	4
ATCAACG	34205	0.0	29.85845	3
TATCAAC	35070	0.0	29.363468	2
CAACGCA	35130	0.0	29.121197	5
AACGCAG	36250	0.0	28.221453	6
ACGCAGA	41820	0.0	24.47292	7
CGCAGAG	43250	0.0	23.653818	8
GTGGTAT	7060	0.0	21.559374	1
GCAGAGT	49275	0.0	20.65805	9
GTACATG	35560	0.0	20.639986	1
TGGTATC	7195	0.0	20.019426	2
TACATGG	35900	0.0	19.893574	2
GAGTACT	27690	0.0	19.87089	12-13
ACATGGG	36215	0.0	19.085695	3
AGAGTAC	44535	0.0	18.826725	10-11
CAGAGTA	48205	0.0	18.499144	10-11
GTACTTT	29835	0.0	18.363272	14-15
AGTACTT	30195	0.0	17.353975	12-13
>>END_MODULE