FastQCFastQC Report
Fri 27 May 2016
SRR2138843_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138843_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12928954
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108950.035.1454621
GTATCAA170000.029.8814771
TCAACGC172900.028.0178184
ATCAACG177800.027.2230433
CAACGCA179950.026.896125
AACGCAG188950.025.6823836
TATCAAC204750.024.1015362
ACGCAGA229900.021.0530157
CGCAGAG235450.020.5203428
GTACATG380000.019.1196521
TACATGG371400.018.8270722
ACATGGG384800.017.7032573
CATGGGG284300.015.8082944
GCAGAGT309450.015.4343469
AGAGTAC253800.014.83268310-11
CAGAGTA295150.014.4032110-11
GTGGTAT74550.013.6280381
GTATAGG39850.013.6044021
GTATAAG56600.013.2739531
GTGTAGC77050.013.1304541
Produced by FastQC (version 0.11.5)