##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138843_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12928954
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.981300884820225	33.0	31.0	34.0	30.0	34.0
2	32.213913128625876	34.0	31.0	34.0	30.0	34.0
3	32.31802410310996	34.0	31.0	34.0	30.0	34.0
4	35.71071263769675	37.0	35.0	37.0	35.0	37.0
5	35.811002885461576	37.0	35.0	37.0	35.0	37.0
6	35.85081391735171	37.0	36.0	37.0	35.0	37.0
7	35.78171598413917	37.0	36.0	37.0	35.0	37.0
8	35.72329602224588	37.0	36.0	37.0	35.0	37.0
9	37.45904471467684	39.0	38.0	39.0	35.0	39.0
10-11	37.45372560688204	39.0	38.0	39.0	35.0	39.0
12-13	37.38310941472914	39.0	38.0	39.0	35.0	39.0
14-15	38.7930337210574	41.0	39.0	41.0	35.0	41.0
16-17	38.792906757963564	41.0	39.0	41.0	35.0	41.0
18-19	38.8006587771911	41.0	38.5	41.0	34.0	41.0
20-21	38.837090042945675	41.0	39.0	41.0	34.0	41.0
22-23	38.79549470771546	40.0	39.0	41.0	34.0	41.0
24-25	38.75876111859909	40.0	38.0	41.0	34.0	41.0
26-27	38.71407303772341	40.0	38.0	41.0	34.0	41.0
28-29	38.63618674089646	40.0	38.0	41.0	34.0	41.0
30-31	38.510069930006416	40.0	38.0	41.0	34.0	41.0
32-33	38.4524263063536	40.0	38.0	41.0	34.0	41.0
34-35	38.295369321849364	40.0	38.0	41.0	33.0	41.0
36-37	38.1745019758409	40.0	38.0	41.0	33.0	41.0
38-39	38.15512782848162	40.0	38.0	41.0	33.0	41.0
40-41	38.067186117695115	40.0	38.0	41.0	33.0	41.0
42-43	37.88461194661586	40.0	37.5	41.0	32.5	41.0
44-45	37.94295851996432	40.0	37.5	41.0	32.5	41.0
46-47	38.15462269245243	40.0	38.0	41.0	33.0	41.0
48-49	38.14586109243868	40.0	38.0	41.0	33.0	41.0
50-51	38.049391534866814	40.0	37.5	41.0	33.0	41.0
52-53	37.968151327635276	40.0	37.0	41.0	33.0	41.0
54-55	37.87869113345215	40.0	37.0	41.0	33.0	41.0
56-57	37.920204690699535	40.0	37.0	41.0	33.0	41.0
58-59	37.72166248461014	40.0	36.5	41.0	32.0	41.0
60-61	37.635462894592465	39.5	36.0	41.0	32.0	41.0
62-63	37.39945425086053	39.0	35.5	41.0	32.0	41.0
64-65	37.18721827692906	39.0	35.0	41.0	31.5	41.0
66-67	36.901406659914784	38.5	35.0	41.0	31.0	41.0
68-69	36.65553109120401	37.5	35.0	40.0	31.0	41.0
70-71	36.354657007192365	37.0	35.0	40.0	31.0	41.0
72-73	36.02060792932936	37.0	35.0	39.0	31.0	41.0
74-75	35.67963231059416	36.0	34.5	39.0	31.0	41.0
76-77	35.350234798438876	36.0	34.0	38.5	30.0	40.0
78-79	35.00009139561315	35.0	34.0	37.0	30.0	39.5
80-81	34.6926515765208	35.0	34.0	37.0	30.0	39.0
82-83	34.39882738743441	35.0	34.0	36.5	30.0	39.0
84-85	34.188023092599444	35.0	34.0	36.0	30.0	37.0
86-87	33.979025077637516	35.0	34.0	36.0	30.0	37.0
88-89	33.758633917479315	35.0	34.0	35.5	29.5	37.0
90-91	33.59955722965129	35.0	34.0	35.0	29.0	36.0
92-93	33.524410845781006	35.0	34.0	35.0	29.0	36.0
94-95	33.51268875636289	35.0	34.0	35.0	29.5	36.0
96-97	33.477516798013156	35.0	33.5	35.0	29.5	36.0
98-99	33.51729131348445	35.0	33.0	35.0	30.0	35.5
100	32.9241241049253	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	15.0
16	51.0
17	355.0
18	1742.0
19	5211.0
20	11518.0
21	21324.0
22	34018.0
23	48102.0
24	64461.0
25	83824.0
26	101148.0
27	113449.0
28	131016.0
29	161397.0
30	190997.0
31	229009.0
32	294251.0
33	392177.0
34	551344.0
35	814066.0
36	1319029.0
37	2382979.0
38	3533383.0
39	2300206.0
40	143881.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.12270025865975	20.31208402474013	14.747024391919098	23.818191324681024
2	14.262135130673167	20.874858599669846	41.88500971602652	22.97799655363047
3	18.00524797132514	25.901935604246557	30.295342075414	25.797474349014305
4	12.443128464309968	18.297852920655988	39.18213278507704	30.076885829957007
5	13.575989223728158	37.953227737544495	35.40762436394145	13.063158674785896
6	29.772327147162386	37.90415354245438	19.226903964801327	13.096615345581911
7	26.637975136275877	32.79896576214169	23.01055477665948	17.552504324922957
8	24.39232507351042	36.69203610618051	21.378013002872347	17.537625817436727
9	25.109388473415862	17.96915681304085	21.331757867043887	35.58969684649939
10-11	23.8436682083341	27.222579144591926	29.057427408409964	19.876325238664013
12-13	24.554410274584193	24.598044978089245	28.977337839854762	21.870206907471804
14-15	21.927288439118932	26.238412232153248	26.34528639832281	25.489012930405014
16-17	21.8673512959571	29.27186628968431	27.018012552387827	21.842769861970762
18-19	21.97318074714505	28.82519915262841	28.08354209833726	21.11807800188928
20-21	22.822852847319698	27.943058636020712	27.831360954300916	21.40272756235867
22-23	22.54713628680799	27.78183678366903	27.666842371932816	22.004184557590172
24-25	22.274003942339984	27.934263751192752	27.702419971877625	22.08931233458964
26-27	22.238326704438364	28.084596324108517	27.61153227609194	22.06554469536118
28-29	22.17279034907218	28.188311173039743	27.625984308825714	22.012914169062363
30-31	22.51627489249862	27.947746164552928	27.567152399244186	21.968826543704267
32-33	22.24410559111116	28.04792714871467	27.572359240624504	22.135608019549665
34-35	22.41422899264273	28.059266567307805	27.564782420923635	21.961722019125833
36-37	22.358005191194586	27.89166005728928	27.645328222568942	22.105006528947193
38-39	22.15354659564184	27.962370576405704	27.79453680270857	22.089546025243887
40-41	22.377180236495498	27.855383991287535	27.719389173714724	22.048046598502243
42-43	22.20140192171762	27.88946263905361	27.69020065610963	22.218934783119142
44-45	22.256625600372644	27.88285451957982	27.711671375671504	22.14884850437603
46-47	22.206788442326662	27.855238222081635	27.617703583037656	22.320269752554044
48-49	22.02390298425338	27.8956630008309	27.80224844369129	22.27818557122443
50-51	22.018556094261708	27.9726553523024	27.761489622517672	22.247298930918223
52-53	22.144270702015614	27.857193358137327	27.71683633087005	22.28169960897701
54-55	22.18105694642664	27.735594826675445	27.747600505344945	22.33574772155297
56-57	22.124937723070172	27.85371642635478	27.704255954531405	22.317089896043647
58-59	22.168546306996838	27.879476813891994	27.677687434660246	22.274289444450922
60-61	22.19534482571175	27.777130778087372	27.74071642282769	22.286807973373186
62-63	22.160587965716793	27.879641806533538	27.64162134345592	22.31814888429375
64-65	22.203024567019572	27.87374118078566	27.54527831673275	22.377955935462012
66-67	22.260214345462966	27.936519195395675	27.422121725059128	22.381144734082227
68-69	22.20521023394818	27.89061993442222	27.487878171824047	22.41629165980556
70-71	22.26064440430147	27.699114310308453	27.564346917444805	22.475894367945273
72-73	22.30512964472323	27.70824101054194	27.515464300932948	22.471165043801893
74-75	22.216755803104956	27.847378785627296	27.560482335572996	22.375383075694753
76-77	22.337231357058254	27.791943929686163	27.494108380171202	22.37671633308438
78-79	22.36629470040825	27.81271974454415	27.463240383528348	22.357745171519248
80-81	22.35101620912697	27.849698251921957	27.470007476590812	22.329278062360252
82-83	22.329942233574958	27.835188809988093	27.574965913469168	22.259903042967785
84-85	22.440548727772043	27.848072468673283	27.573546015742345	22.137832787812332
86-87	22.198152380417817	27.888147145780383	27.685018809309113	22.228681664492687
88-89	22.313648496395036	27.91605264197835	27.62301157522378	22.147287286402833
90-91	22.293213523013993	27.931710171543983	27.69645068270853	22.07862562273349
92-93	22.266928134803745	27.919929670137257	27.696750690588136	22.11639150447087
94-95	22.148221612856467	27.997718218159473	27.7177158968212	22.136344272162862
96-97	22.19248936027296	28.13695550444249	27.786028178650636	21.884526956633916
98-99	23.231432369341178	30.06147340452347	27.98965401491287	18.717440211222485
100	22.21201652538044	31.10893425913856	21.778316649471634	24.900732566009363
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4041.0
1	3096.5
2	2387.0
3	2604.5
4	2585.5
5	2760.5
6	3237.5
7	3829.5
8	4340.0
9	4863.5
10	5367.5
11	5690.0
12	6093.0
13	6880.5
14	8074.0
15	9768.0
16	11868.0
17	14202.0
18	17019.0
19	20475.0
20	24950.0
21	31041.5
22	39285.0
23	50191.5
24	64945.0
25	84241.0
26	108882.5
27	137172.0
28	165742.0
29	196167.0
30	226212.5
31	256094.0
32	287301.0
33	317547.0
34	348910.0
35	377517.5
36	407010.0
37	435733.0
38	454812.5
39	471487.5
40	492200.5
41	509881.5
42	521706.5
43	532265.0
44	539830.5
45	545965.0
46	548617.0
47	546724.5
48	542866.0
49	533341.5
50	520880.0
51	504271.5
52	482190.5
53	456724.5
54	428669.0
55	398701.0
56	366036.0
57	332463.5
58	297542.0
59	260659.0
60	221077.0
61	182166.5
62	146511.0
63	114977.5
64	88662.0
65	67919.0
66	52126.0
67	40564.5
68	31948.5
69	24841.5
70	19522.5
71	15286.5
72	11857.0
73	9184.5
74	7126.0
75	5518.5
76	4241.0
77	3284.5
78	2556.0
79	1960.5
80	1470.0
81	1122.0
82	877.0
83	621.0
84	451.5
85	348.5
86	279.0
87	229.0
88	157.5
89	103.0
90	79.5
91	68.0
92	57.0
93	37.5
94	22.5
95	15.5
96	11.5
97	9.0
98	8.5
99	13.0
100	37.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.022337460555587094
3	0.012939948583620917
4	0.005367796961765044
5	0.009993074459078438
6	0.00923508583911738
7	0.0030938311018818693
8	0.009242820416872084
9	0.005553426827877955
10-11	0.004083857054484067
12-13	0.009053323261881819
14-15	0.005151228784633312
16-17	0.0038479524329655744
18-19	0.005735189405113515
20-21	0.004642891605272808
22-23	0.010634883991301773
24-25	0.009681336803944717
26-27	0.02298086787497617
28-29	0.019775825588319168
30-31	0.0162489241890504
32-33	0.01296162548754095
34-35	0.0090302700273274
36-37	0.010527935218105291
38-39	0.005130799987723853
40-41	0.006085762510848791
42-43	0.0041198341768730225
44-45	0.007107652772583877
46-47	0.009975869734200369
48-49	0.00740070964222589
50-51	0.009739205669570592
52-53	0.0010006361421244522
54-55	0.0025389631766120123
56-57	5.698849972075635E-4
58-59	0.0028477528818189684
60-61	0.001027950437729216
62-63	0.007159601687208352
64-65	0.0037623788290168433
66-67	0.003746337325080381
68-69	0.004977942753219366
70-71	7.495152100141618E-4
72-73	5.690561513692948E-4
74-75	0.0
76-77	3.204205235570651E-5
78-79	4.7239860623885725E-4
80-81	2.0162606261623975E-4
82-83	0.0
84-85	8.657334476935154E-4
86-87	0.0037141362563829702
88-89	0.022475923769637744
90-91	0.0028789404049773255
92-93	0.001278448090839771
94-95	0.0010721761391780397
96-97	4.908423489602066E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	4118.0
20-21	7583.0
22-23	8577.0
24-25	10305.0
26-27	12654.0
28-29	16914.0
30-31	24205.0
32-33	28681.0
34-35	37503.0
36-37	55365.0
38-39	63256.0
40-41	59933.0
42-43	66106.0
44-45	77558.0
46-47	85064.0
48-49	79958.0
50-51	79505.0
52-53	78736.0
54-55	88787.0
56-57	96962.0
58-59	101816.0
60-61	101166.0
62-63	102670.0
64-65	108546.0
66-67	114161.0
68-69	115618.0
70-71	115841.0
72-73	115127.0
74-75	118483.0
76-77	125220.0
78-79	131299.0
80-81	134357.0
82-83	133050.0
84-85	138112.0
86-87	168376.0
88-89	150329.0
90-91	156085.0
92-93	164811.0
94-95	202732.0
96-97	469673.0
98-99	825225.0
100-101	8154487.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.34735889966457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.17024673338841	76.95792410025807
2	6.81971675737799	11.640896273719918
3	1.676447842941019	4.2924118718416695
4	0.6200957569415932	2.116941404794133
5	0.2711268319080422	1.1569979515092355
6	0.14244038812390306	0.7294146556210963
7	0.08260508634173497	0.4935088165662084
8	0.05326121933938486	0.3636563521913788
9	0.03729848533278495	0.28649944926999654
>10	0.12292187533738698	1.5911383037022848
>50	0.0030210244174682646	0.1676299302236758
>100	7.284624068599977E-4	0.11274787146646215
>500	5.969262055529757E-5	0.03907520098287301
>1k	2.9843522945532142E-5	0.05115781785300429
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.187662203763738E-5	0.0	1.5469155509409346E-5	0.0
3	0.0	6.187662203763738E-5	0.0	2.3203733264114017E-5	0.0
4	0.0	9.281493305645607E-5	0.0	4.6407466528228034E-5	0.0
5	7.734577754704673E-6	9.281493305645607E-5	0.0	6.187662203763738E-5	0.0
6	3.867288877352336E-5	9.281493305645607E-5	0.0	6.187662203763738E-5	7.734577754704673E-6
7	3.867288877352336E-5	1.0054951081116075E-4	0.0	7.734577754704673E-5	7.734577754704673E-6
8	3.867288877352336E-5	1.0828408856586543E-4	0.0	8.50803553017514E-5	7.734577754704673E-6
9	3.867288877352336E-5	2.1656817713173085E-4	0.0	1.0828408856586543E-4	1.5469155509409346E-5
10-11	4.6407466528228034E-5	2.2430275488643551E-4	0.0	1.5082426621674112E-4	1.5469155509409346E-5
12-13	4.6407466528228034E-5	2.2817004376378785E-4	0.0	1.817625772355598E-4	1.933644438676168E-5
14-15	4.6407466528228034E-5	2.9004666580142524E-4	0.0	2.2430275488643551E-4	2.7071022141466353E-5
16-17	5.414204428293271E-5	3.093831101881869E-4	0.0	3.055158213108346E-4	3.093831101881869E-5
18-19	5.414204428293271E-5	3.519232878390626E-4	0.0	3.944634654899383E-4	3.093831101881869E-5
20-21	5.414204428293271E-5	3.9833075436729066E-4	0.0	5.220839984425654E-4	3.480559989617103E-5
22-23	5.414204428293271E-5	4.331363542634617E-4	0.0	8.237325308760477E-4	3.867288877352336E-5
24-25	5.414204428293271E-5	4.60207376404928E-4	0.0	0.0013767548403374318	4.2540177650875695E-5
26-27	5.414204428293271E-5	4.71809243036985E-4	0.0	0.002405453681713153	4.6407466528228034E-5
28-29	5.414204428293271E-5	4.834111096690421E-4	0.0	0.009030119528617705	4.6407466528228034E-5
30-31	6.961119979234206E-5	5.568895983387365E-4	0.0	0.03059412230873433	4.6407466528228034E-5
32-33	8.50803553017514E-5	5.568895983387365E-4	0.0	0.09396738514190708	5.414204428293271E-5
34-35	8.50803553017514E-5	6.110316426216691E-4	0.0	0.17345177343812962	6.187662203763738E-5
36-37	8.50803553017514E-5	6.806428424140112E-4	0.0	0.28656610581180814	6.187662203763738E-5
38-39	8.894764417910374E-5	6.961119979234206E-4	0.0	0.41015305646535677	6.187662203763738E-5
40-41	9.668222193380841E-5	7.57988619961058E-4	0.0	0.5191255224513909	6.187662203763738E-5
42-43	1.0054951081116075E-4	7.773250643478197E-4	0.0	0.6634218050431613	6.574391091498973E-5
44-45	1.0054951081116075E-4	8.314671086307524E-4	0.0	0.7794211349193445	6.961119979234206E-5
46-47	1.0054951081116075E-4	8.817418640363327E-4	0.0	0.9034102836161378	7.34784886696944E-5
48-49	1.0054951081116075E-4	8.894764417910374E-4	0.0	0.993460105125287	9.281493305645607E-5
50-51	1.0441679968851308E-4	0.001032566130253074	0.0	1.102099984267869	9.281493305645607E-5
52-53	1.0828408856586543E-4	0.0010596371523945401	0.0	1.2144408588660769	1.0054951081116075E-4
54-55	1.0828408856586543E-4	0.0010751063079039495	0.0	1.3211238898367186	1.1215137744321776E-4
56-57	1.0828408856586543E-4	0.0010983100411680637	0.0	1.411235587967905	1.2375324407527477E-4
58-59	1.0828408856586543E-4	0.001113779196677473	0.0	1.472895641828411	1.3148782182997943E-4
60-61	1.2375324407527477E-4	0.001148584796573644	0.0	1.5099520038512009	1.3148782182997943E-4
62-63	1.3922239958468412E-4	0.0011679212409604056	0.0	1.5341534976456719	1.3922239958468412E-4
64-65	1.3922239958468412E-4	0.0013071436405450898	0.0	1.5432416265074498	1.3922239958468412E-4
66-67	1.3922239958468412E-4	0.0013303473738092038	0.0	1.5507364323517587	1.3922239958468412E-4
68-69	1.3922239958468412E-4	0.0013458165293186131	0.0	1.5577130214865025	1.3922239958468412E-4
70-71	1.3922239958468412E-4	0.0013767548403374318	0.0	1.5639316220012849	1.5082426621674112E-4
72-73	1.469569773393888E-4	0.0013999585736015458	0.0	1.5696784132730306	1.6242613284879814E-4
74-75	1.5469155509409345E-4	0.0014541006178844784	0.0	1.5751815653455028	1.6242613284879814E-4
76-77	1.6242613284879814E-4	0.0015043753732900589	0.0	1.5789405701342893	1.6242613284879814E-4
78-79	1.701607106035028E-4	0.0015314463954315253	0.0	1.5811217210611161	1.6242613284879814E-4
80-81	1.701607106035028E-4	0.0015314463954315253	0.0	1.5822973768798312	1.7402799948085514E-4
82-83	1.7402799948085514E-4	0.0015314463954315253	0.0	1.583074701944179	1.7789528835820747E-4
84-85	1.7789528835820747E-4	0.0015353136843088777	0.0	1.5833260757212069	1.7789528835820747E-4
86-87	1.7789528835820747E-4	0.001562384706450344	0.0	1.5834304925208953	1.7789528835820747E-4
88	1.8562986611291214E-4	0.0016165267507332766	0.0	1.58343822709865	1.7789528835820747E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10895	0.0	35.145462	1
GTATCAA	17000	0.0	29.881477	1
TCAACGC	17290	0.0	28.017818	4
ATCAACG	17780	0.0	27.223043	3
CAACGCA	17995	0.0	26.89612	5
AACGCAG	18895	0.0	25.682383	6
TATCAAC	20475	0.0	24.101536	2
ACGCAGA	22990	0.0	21.053015	7
CGCAGAG	23545	0.0	20.520342	8
GTACATG	38000	0.0	19.119652	1
TACATGG	37140	0.0	18.827072	2
ACATGGG	38480	0.0	17.703257	3
CATGGGG	28430	0.0	15.808294	4
GCAGAGT	30945	0.0	15.434346	9
AGAGTAC	25380	0.0	14.832683	10-11
CAGAGTA	29515	0.0	14.40321	10-11
GTGGTAT	7455	0.0	13.628038	1
GTATAGG	3985	0.0	13.604402	1
GTATAAG	5660	0.0	13.273953	1
GTGTAGC	7705	0.0	13.130454	1
>>END_MODULE