FastQCFastQC Report
Fri 27 May 2016
SRR2138842_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138842_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8761774
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73600.038.9388051
GTATCAA118100.032.5664941
TCAACGC127700.028.9099084
ATCAACG129150.028.587453
CAACGCA131250.028.0270255
AACGCAG137200.026.9720426
TATCAAC144550.026.03072
TCGGTGG67600.025.07544194
ACGCAGA162450.022.6206137
CGCAGAG165600.022.1636588
GTACATG265950.020.0150641
TACATGG261850.019.422762
ACATGGG273150.018.665833
GCAGAGT210400.017.4859859
CGGTGGT21400.017.4262694
CATGGGG194300.016.8290064
CTCGGTG87150.016.72731894
AGAGTAC180450.016.36813410-11
ACCGAAC9050.016.028058
TCTCGGT94050.015.81839392-93
Produced by FastQC (version 0.11.5)