##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138842_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8761774
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.988370505790265	33.0	31.0	34.0	30.0	34.0
2	32.208225183621494	34.0	31.0	34.0	30.0	34.0
3	32.30865826943265	34.0	31.0	34.0	30.0	34.0
4	35.693262232054835	37.0	35.0	37.0	35.0	37.0
5	35.80422012711124	37.0	35.0	37.0	35.0	37.0
6	35.84111368314225	37.0	36.0	37.0	35.0	37.0
7	35.769362231895045	37.0	36.0	37.0	35.0	37.0
8	35.71136838270424	37.0	36.0	37.0	35.0	37.0
9	37.4407492135725	39.0	38.0	39.0	35.0	39.0
10-11	37.43855045793238	39.0	38.0	39.0	35.0	39.0
12-13	37.36972529763949	39.0	38.0	39.0	35.0	39.0
14-15	38.783727872917055	41.0	39.0	41.0	35.0	41.0
16-17	38.79674093397068	41.0	39.0	41.0	35.0	41.0
18-19	38.81347441739538	41.0	38.5	41.0	34.0	41.0
20-21	38.854115260280985	41.0	39.0	41.0	34.0	41.0
22-23	38.816524377334204	40.0	39.0	41.0	34.0	41.0
24-25	38.78046508758921	40.0	38.0	41.0	34.0	41.0
26-27	38.73453537840439	40.0	38.0	41.0	34.0	41.0
28-29	38.65809713922606	40.0	38.0	41.0	34.0	41.0
30-31	38.534254911615676	40.0	38.0	41.0	34.0	41.0
32-33	38.47736049607347	40.0	38.0	41.0	34.0	41.0
34-35	38.318581760140006	40.0	38.0	41.0	33.0	41.0
36-37	38.20488101618156	40.0	38.0	41.0	33.0	41.0
38-39	38.1924073431283	40.0	38.0	41.0	33.0	41.0
40-41	38.10625629875889	40.0	38.0	41.0	33.0	41.0
42-43	37.92967265122266	40.0	37.5	41.0	33.0	41.0
44-45	37.99446190176603	40.0	37.5	41.0	32.5	41.0
46-47	38.21070420863352	40.0	38.0	41.0	33.0	41.0
48-49	38.210624885473855	40.0	38.0	41.0	33.0	41.0
50-51	38.126189158662015	40.0	38.0	41.0	33.0	41.0
52-53	38.05923929673337	40.0	37.0	41.0	33.0	41.0
54-55	37.98231263608739	40.0	37.0	41.0	33.0	41.0
56-57	38.037698680769	40.0	37.0	41.0	33.0	41.0
58-59	37.85341940340102	40.0	37.0	41.0	33.0	41.0
60-61	37.77662350942016	40.0	36.0	41.0	33.0	41.0
62-63	37.55022205187457	39.0	36.0	41.0	32.0	41.0
64-65	37.34002619927273	39.0	35.0	41.0	32.0	41.0
66-67	37.05419496866597	39.0	35.0	41.0	31.5	41.0
68-69	36.80660401898611	38.0	35.0	40.0	31.0	41.0
70-71	36.49207654294045	37.0	35.0	40.0	31.0	41.0
72-73	36.12929229941389	37.0	35.0	39.5	31.0	41.0
74-75	35.762958129316985	36.0	34.5	39.0	31.0	41.0
76-77	35.383295409291065	36.0	34.0	39.0	30.0	40.5
78-79	34.98994915427318	35.0	34.0	37.5	30.0	39.5
80-81	34.634928516607346	35.0	34.0	37.0	30.0	39.0
82-83	34.30302585163969	35.0	34.0	36.5	30.0	39.0
84-85	34.06465732632908	35.0	34.0	36.0	29.5	37.0
86-87	33.82392616967261	35.0	34.0	36.0	29.0	37.0
88-89	33.56810397868822	35.0	33.0	35.0	29.0	37.0
90-91	33.36706557803754	35.0	33.0	35.0	29.0	36.0
92-93	33.26279850358755	35.0	33.0	35.0	29.0	36.0
94-95	33.24163752344394	35.0	33.0	35.0	29.0	36.0
96-97	33.214070287271824	35.0	33.0	35.0	29.0	36.0
98-99	33.31798201617059	35.0	33.0	35.0	29.0	35.0
100	32.69496736799528	34.0	32.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	3.0
16	41.0
17	244.0
18	1131.0
19	3280.0
20	7731.0
21	14647.0
22	23587.0
23	32954.0
24	43692.0
25	56232.0
26	66941.0
27	73973.0
28	85911.0
29	106038.0
30	125897.0
31	153194.0
32	200721.0
33	274739.0
34	392856.0
35	588242.0
36	964245.0
37	1705493.0
38	2406279.0
39	1342024.0
40	91677.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.75409842801241	20.781419379226172	14.742585234451381	23.72189695831004
2	14.092956672427848	21.312567210592047	41.87043510225143	22.72404101472868
3	17.467673394125534	26.522924121153846	30.81141233260542	25.197990152115203
4	12.177406652407052	18.6231101635138	39.705376118265015	29.494107065814134
5	13.1805008127572	38.354617820345105	35.76406767372235	12.70081369317534
6	29.22206696226681	38.5787519915114	19.39663054190341	12.802550504318374
7	26.118271768193992	33.24247023894106	23.50915073880336	17.13010725406159
8	24.152774334467	36.78625874734104	21.746231115628127	17.31473580256383
9	25.0904802630385	17.801381826424677	21.62544772164597	35.48269018889086
10-11	23.516971871066385	27.419465569721634	29.531354122991544	19.53220843622044
12-13	24.256872942939637	24.924480523594095	29.331542058341615	21.487104475124656
14-15	21.67293720742914	26.56292605502424	26.678987081769122	25.0851496557775
16-17	21.53970553633532	29.523596795486412	27.391810343819056	21.544887324359216
18-19	21.643384517593915	29.13797333475474	28.41757421839532	20.80106792925602
20-21	22.592447298070308	28.235571931988535	28.08122309322175	21.090757676719402
22-23	22.302286431021027	28.091025090649275	27.90946373497744	21.697224743352265
24-25	22.007556520830857	28.26386569889871	27.940918108076417	21.787659672194017
26-27	21.980455189465033	28.452454706927988	27.825173715053403	21.74191638855357
28-29	21.90556696535446	28.509811647328693	27.858056283932342	21.7265651033845
30-31	22.22650183171302	28.241670810028534	27.807789543261833	21.724037814996613
32-33	21.97847873565804	28.321919061664186	27.82390391131647	21.8756982913613
34-35	22.141562950513833	28.29722162008213	27.8307737806548	21.730441648749242
36-37	22.103924215084017	28.0829037540773	27.925375177291752	21.887796853546934
38-39	21.845750441837602	28.074299644950536	28.153309447384373	21.926640465827493
40-41	22.01001713327169	27.862413507219664	28.195536271055477	21.932033088453178
42-43	21.844457301815705	27.868662359686375	28.152102844134134	22.134777494363785
44-45	21.822429824903665	27.835443104318795	28.237133328169794	22.104993742607746
46-47	21.74295506673595	27.718153682505193	28.25028007112436	22.2886111796345
48-49	21.485076103878097	27.68222172737439	28.48450009866783	22.34820207007968
50-51	21.337939246236996	27.69709482289252	28.561089514964316	22.40387641590617
52-53	21.31470879865531	27.61174653450547	28.628158445131557	22.445386221707658
54-55	21.29735858929228	27.55433842132463	28.648849995864456	22.499452993518634
56-57	21.26074941642416	27.632489282015477	28.47855968336384	22.628201618196524
58-59	21.218755872237303	27.678141868015437	28.42123717626961	22.68186508347765
60-61	21.21154041273334	27.41389528930946	28.512480815196657	22.862083482760546
62-63	21.24281231077935	27.406668081818292	28.41349548930758	22.93702411809478
64-65	21.386759801283876	27.50096282842478	28.239836558126598	22.872440812164747
66-67	21.251940978619928	27.64316051134229	27.943305089076116	23.16159342096167
68-69	21.326815222492037	27.74785906179904	27.738139604812957	23.187186110895965
70-71	21.47343126411504	27.683615611091213	27.558935132445384	23.28401799234837
72-73	21.66910892566129	27.629619167697495	27.11259953699883	23.58867236964238
74-75	21.73277895547006	27.611436191816747	26.888386448666573	23.76739840404662
76-77	22.01100901037757	27.32603210820702	27.035181127961934	23.627777753453476
78-79	22.183963904902015	27.064275472220338	27.18742623514728	23.56433438773036
80-81	22.28788782198566	26.96536600426243	27.20726501764208	23.53948115610983
82-83	22.26308705194628	27.079209842558466	27.09286275077316	23.564840354722097
84-85	22.353530275992693	26.8731471915935	27.289626844571096	23.483695687842708
86-87	22.086560742344194	27.06413268567577	27.58555291157505	23.26375366040499
88-89	22.25628381598692	27.24828140728618	27.32701511889838	23.168419657828522
90-91	22.211458404465724	27.501841842852315	27.62262375409634	22.664075998585623
92-93	21.890679909892054	27.455108334119625	27.94722071455592	22.7069910414324
94-95	21.78382238862539	27.44709920076889	28.19601677334495	22.57306163726077
96-97	21.96100330918301	27.35115463968174	28.46264710152362	22.225194949611637
98-99	23.36974737668988	29.341396908633577	28.62145305990909	18.667402654767454
100	22.8822874529263	30.517245574569785	22.265987425384726	24.33447954711919
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3029.0
1	2329.5
2	1803.5
3	1928.5
4	1888.0
5	2108.5
6	2606.0
7	3115.0
8	3500.5
9	3900.5
10	4310.5
11	4586.5
12	4905.5
13	5437.0
14	6182.5
15	7197.0
16	8440.0
17	10007.5
18	11903.0
19	14059.5
20	16648.5
21	20090.5
22	25135.5
23	31794.0
24	40541.5
25	52250.5
26	67187.5
27	84460.0
28	102399.5
29	122625.5
30	142655.0
31	162727.5
32	184075.5
33	203961.5
34	224233.0
35	242471.0
36	262519.0
37	283462.5
38	299017.0
39	311827.5
40	326385.0
41	339102.0
42	348192.0
43	357794.0
44	363512.0
45	368624.0
46	373158.0
47	372469.5
48	368227.0
49	360070.5
50	349572.0
51	336033.5
52	318091.0
53	296552.0
54	274117.0
55	250150.0
56	223877.0
57	196784.5
58	171505.5
59	148799.0
60	124596.0
61	101272.5
62	80835.5
63	63031.5
64	48192.5
65	36632.5
66	28265.0
67	22456.5
68	18059.5
69	14248.0
70	11316.0
71	8946.5
72	7067.5
73	5525.0
74	4333.0
75	3505.0
76	2738.0
77	2134.5
78	1664.0
79	1248.0
80	946.0
81	722.5
82	574.5
83	418.5
84	308.5
85	249.0
86	208.0
87	170.0
88	119.0
89	76.0
90	51.5
91	42.0
92	37.0
93	24.0
94	12.0
95	8.5
96	7.5
97	8.5
98	9.0
99	13.0
100	31.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.02275794833329415
3	0.0126800805407672
4	0.004953334792702939
5	0.009758297805900951
6	0.009587099598779882
7	0.0027505845277451804
8	0.009427314605466884
9	0.005375623703601577
10-11	0.004000331439728986
12-13	0.009005025694568247
14-15	0.00496474800651101
16-17	0.0036465218116787765
18-19	0.005929164573293034
20-21	0.004578756408617585
22-23	0.010225670843996022
24-25	0.00974099082683348
26-27	0.023411756615222976
28-29	0.02001748250746947
30-31	0.015899720257843905
32-33	0.012926325288735326
34-35	0.008944002257986463
36-37	0.010508491605232075
38-39	0.005067663430554759
40-41	0.005960911050089645
42-43	0.003931107229498098
44-45	0.006941176210259874
46-47	0.009971345501873563
48-49	0.00771996453488002
50-51	0.010041428935690409
52-53	8.961231603557925E-4
54-55	0.0026455055939538404
56-57	5.581334466520483E-4
58-59	0.0028718516811013506
60-61	9.727535847738241E-4
62-63	0.006947269808624575
64-65	0.003689887785156165
66-67	0.003727020962088382
68-69	0.005047788157633859
70-71	7.612892285739598E-4
72-73	5.708983097314457E-4
74-75	0.0
76-77	1.9036407510091198E-5
78-79	3.7263760607322204E-4
80-81	1.562568565834204E-4
82-83	0.0
84-85	9.442679020637477E-4
86-87	0.003309500616105079
88-89	0.021245054525158465
90-91	0.002749787633688984
92-93	0.0012046757867596898
94-95	0.001099535811401714
96-97	4.486956114375097E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2671.0
20-21	5135.0
22-23	5784.0
24-25	7047.0
26-27	8693.0
28-29	11329.0
30-31	14533.0
32-33	14708.0
34-35	17762.0
36-37	24173.0
38-39	28308.0
40-41	20697.0
42-43	11504.0
44-45	11810.0
46-47	12211.0
48-49	12634.0
50-51	12695.0
52-53	12947.0
54-55	13176.0
56-57	13948.0
58-59	14910.0
60-61	16008.0
62-63	47784.0
64-65	82118.0
66-67	86277.0
68-69	86783.0
70-71	87419.0
72-73	85592.0
74-75	89859.0
76-77	95948.0
78-79	101261.0
80-81	106124.0
82-83	106195.0
84-85	113599.0
86-87	138796.0
88-89	133436.0
90-91	139980.0
92-93	151373.0
94-95	179031.0
96-97	350834.0
98-99	615256.0
100-101	5671426.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.24983108454036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.85085985884504	74.85669934823862
2	7.657682492339858	12.903169129573499
3	2.021758100534603	5.109983353915245
4	0.6969506725750446	2.3487190575481725
5	0.31532005542832703	1.3282830703702226
6	0.16426680461137838	0.8303670324783494
7	0.09871701101299482	0.5821824052110868
8	0.05516461028281601	0.37180872785373975
9	0.039065308535258546	0.2962121080824887
>10	0.09874838380948864	1.2007432107715756
>50	0.0010475127100550627	0.060124896623902954
>100	3.565751728901589E-4	0.05957156974304858
>500	2.087268894793107E-5	0.009126721489163962
>1k	4.1741453392500775E-5	0.04300936810088922
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1413213808071288E-5
2	0.0	1.1413213808071288E-5	0.0	1.1413213808071288E-5	1.1413213808071288E-5
3	0.0	1.1413213808071288E-5	0.0	2.2826427616142577E-5	1.1413213808071288E-5
4	0.0	4.5652855232285153E-5	0.0	3.423964142421386E-5	1.1413213808071288E-5
5	0.0	4.5652855232285153E-5	0.0	4.5652855232285153E-5	1.1413213808071288E-5
6	2.2826427616142577E-5	4.5652855232285153E-5	0.0	5.706606904035644E-5	3.423964142421386E-5
7	2.2826427616142577E-5	4.5652855232285153E-5	0.0	5.706606904035644E-5	3.423964142421386E-5
8	2.2826427616142577E-5	7.989249665649901E-5	0.0	9.130571046457031E-5	3.423964142421386E-5
9	2.2826427616142577E-5	1.7119820712106933E-4	0.0	1.3695856569685545E-4	5.706606904035644E-5
10-11	3.423964142421386E-5	1.7119820712106933E-4	0.0	1.6549160021703366E-4	5.706606904035644E-5
12-13	3.423964142421386E-5	1.7119820712106933E-4	0.0	1.8261142092914061E-4	6.847928284842772E-5
14-15	3.423964142421386E-5	2.2826427616142575E-4	0.0	1.9973124164124754E-4	1.0271892427264159E-4
16-17	9.130571046457031E-5	2.2826427616142575E-4	0.0	2.682105244896753E-4	1.1413213808071288E-4
18-19	9.130571046457031E-5	2.3967748996949704E-4	0.0	2.9674355900985346E-4	1.2554535188878416E-4
20-21	1.0271892427264159E-4	2.625039175856396E-4	0.0	3.252765935300317E-4	1.312519587928198E-4
22-23	1.0842553117667723E-4	3.5380962805020994E-4	0.0	6.790862215802416E-4	1.6549160021703366E-4
24-25	1.1413213808071288E-4	3.8234266257038813E-4	0.0	0.0017405151057308713	1.8261142092914061E-4
26-27	1.1413213808071288E-4	3.880492694744238E-4	0.0	0.003988918225920915	2.0543784854528318E-4
28-29	1.1413213808071288E-4	3.880492694744238E-4	0.0	0.018660604576196553	2.1685106235335447E-4
30-31	1.1413213808071288E-4	4.793549799389941E-4	0.0	0.0686676008762609	2.225576692573901E-4
32-33	1.1413213808071288E-4	4.793549799389941E-4	0.0	0.19915487434393994	2.3967748996949704E-4
34-35	1.1413213808071288E-4	5.307144420753149E-4	0.0	0.3865256054310463	2.3967748996949704E-4
36-37	1.1413213808071288E-4	5.934871180197069E-4	0.0	0.666976801729878	2.5679731068160394E-4
38-39	1.1413213808071288E-4	5.934871180197069E-4	0.0	1.0237481587632824	2.682105244896753E-4
40-41	1.1413213808071288E-4	6.619664008681347E-4	0.0	1.3779058898346386	2.853303452017822E-4
42-43	1.1413213808071288E-4	6.847928284842773E-4	0.0	1.8754078797284661	3.0815677281792475E-4
44-45	1.1413213808071288E-4	7.875117527569188E-4	0.0	2.505605599961834	3.4239641424213866E-4
46-47	1.1413213808071288E-4	8.559910356053465E-4	0.0	3.212226199854048	3.766360556663525E-4
48-49	1.1983874498474852E-4	8.616976425093822E-4	0.0	3.9020066027724525	3.994624832824951E-4
50-51	1.312519587928198E-4	9.644165667820238E-4	0.0	4.582861872492945	3.994624832824951E-4
52-53	1.3695856569685545E-4	9.701231736860595E-4	0.0	5.246369057225169	4.394087316107446E-4
54-55	1.3695856569685545E-4	9.701231736860595E-4	0.0	5.937998400780481	5.078880144591723E-4
56-57	1.3695856569685545E-4	9.92949601302202E-4	0.0	6.6813980821692045	5.478342627874218E-4
58-59	1.3695856569685545E-4	9.986562082062375E-4	0.0	7.437574856416064	5.535408696914575E-4
60-61	1.5407838640896237E-4	0.0010214826358223802	0.0	8.182903370938352	5.763672973076E-4
62-63	1.940246347372119E-4	0.0010614288841506298	0.0	8.878356141119367	6.163135456358495E-4
64-65	1.940246347372119E-4	0.0011755610222313427	0.0	9.067530159988149	6.163135456358495E-4
66-67	1.940246347372119E-4	0.0011983874498474852	0.0	9.077037367090272	6.220201525398852E-4
68-69	1.940246347372119E-4	0.001215507270559592	0.0	9.084598621238118	6.448465801560277E-4
70-71	1.9973124164124754E-4	0.0012383336981757346	0.0	9.090824529370423	6.619664008681347E-4
72-73	2.1685106235335447E-4	0.0012497469119838061	0.0	9.097107503571765	7.190324699084911E-4
74-75	2.1685106235335447E-4	0.0012896931603120555	0.0	9.103065201179579	7.190324699084911E-4
76-77	2.1685106235335447E-4	0.0013752922638725902	0.0	9.107185371364292	7.190324699084911E-4
78-79	2.1685106235335447E-4	0.0014038252983927684	0.0	9.109587852870892	7.190324699084911E-4
80-81	2.1685106235335447E-4	0.0014152385122008396	0.0	9.110694934610274	7.36152290620598E-4
82-83	2.1685106235335447E-4	0.0014380649398169822	0.0	9.11169359081848	7.703919320448119E-4
84-85	2.1685106235335447E-4	0.001460891367433125	0.0	9.112018867412012	7.760985389488476E-4
86-87	2.1685106235335447E-4	0.001472304581241196	0.0	9.112184359012229	7.818051458528832E-4
88	2.1685106235335447E-4	0.0015978499331299803	0.0	9.112195772226036	7.875117527569188E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7360	0.0	38.938805	1
GTATCAA	11810	0.0	32.566494	1
TCAACGC	12770	0.0	28.909908	4
ATCAACG	12915	0.0	28.58745	3
CAACGCA	13125	0.0	28.027025	5
AACGCAG	13720	0.0	26.972042	6
TATCAAC	14455	0.0	26.0307	2
TCGGTGG	6760	0.0	25.075441	94
ACGCAGA	16245	0.0	22.620613	7
CGCAGAG	16560	0.0	22.163658	8
GTACATG	26595	0.0	20.015064	1
TACATGG	26185	0.0	19.42276	2
ACATGGG	27315	0.0	18.66583	3
GCAGAGT	21040	0.0	17.485985	9
CGGTGGT	2140	0.0	17.42626	94
CATGGGG	19430	0.0	16.829006	4
CTCGGTG	8715	0.0	16.727318	94
AGAGTAC	18045	0.0	16.368134	10-11
ACCGAAC	905	0.0	16.02805	8
TCTCGGT	9405	0.0	15.818393	92-93
>>END_MODULE