FastQCFastQC Report
Fri 27 May 2016
SRR2138841_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138841_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8573840
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70950.038.4473721
GTATCAA113800.030.8191971
TCAACGC126900.026.2280524
ATCAACG128950.025.9096343
CAACGCA132700.025.1438625
AACGCAG138150.024.2127936
TATCAAC141400.023.9833452
ACGCAGA166500.019.9419867
GTACATG268500.019.7015131
CGCAGAG169350.019.5816758
TACATGG267650.019.0986062
ACATGGG269650.018.2011533
CATGGGG198150.016.71344
ACCGAAC11250.015.843868
GGACCGA14350.015.5978266
GCAGAGT211200.015.3284999
AGAGTAC177800.015.14201210-11
AGGACCG18950.014.6545545
CAGAGTA202800.014.51197710-11
GTATAAT50650.014.2417371
Produced by FastQC (version 0.11.5)