##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138841_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8573840
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78376760004852	33.0	31.0	34.0	30.0	34.0
2	32.018903548468366	34.0	31.0	34.0	30.0	34.0
3	32.11307792074496	34.0	31.0	34.0	30.0	34.0
4	35.53677640357179	37.0	35.0	37.0	33.0	37.0
5	35.6561811277094	37.0	35.0	37.0	35.0	37.0
6	35.69122376904631	37.0	35.0	37.0	35.0	37.0
7	35.61906112080468	37.0	35.0	37.0	33.0	37.0
8	35.55132134492829	37.0	35.0	37.0	33.0	37.0
9	37.25020539221632	39.0	37.0	39.0	34.0	39.0
10-11	37.24817217256212	39.0	37.0	39.0	34.0	39.0
12-13	37.16896227361369	39.0	37.0	39.0	34.0	39.0
14-15	38.52762198734756	40.0	38.0	41.0	34.0	41.0
16-17	38.534142811155796	40.0	38.0	41.0	34.0	41.0
18-19	38.54685152743694	40.0	38.0	41.0	34.0	41.0
20-21	38.58665719302892	40.0	38.0	41.0	34.0	41.0
22-23	38.54501431273329	40.0	38.0	41.0	34.0	41.0
24-25	38.50772454325667	40.0	38.0	41.0	34.0	41.0
26-27	38.460409647339695	40.0	38.0	41.0	33.5	41.0
28-29	38.37918145099255	40.0	38.0	41.0	33.0	41.0
30-31	38.254656368168824	40.0	38.0	41.0	33.0	41.0
32-33	38.19370486195153	40.0	38.0	41.0	33.0	41.0
34-35	38.03713791711917	40.0	38.0	41.0	33.0	41.0
36-37	37.913160353502406	40.0	38.0	41.0	32.0	41.0
38-39	37.89837292357486	40.0	37.0	41.0	32.0	41.0
40-41	37.81012215552905	40.0	37.0	41.0	32.0	41.0
42-43	37.623625223384636	40.0	37.0	41.0	31.5	41.0
44-45	37.6783308052258	40.0	37.0	41.0	31.5	41.0
46-47	37.894346982175605	40.0	37.0	41.0	32.0	41.0
48-49	37.88278567234218	40.0	37.0	41.0	32.0	41.0
50-51	37.78606242455341	40.0	37.0	41.0	32.0	41.0
52-53	37.70275631413874	40.0	37.0	41.0	32.0	41.0
54-55	37.61248453862811	40.0	36.0	41.0	32.0	41.0
56-57	37.65986568939914	40.0	36.0	41.0	32.0	41.0
58-59	37.46104586404811	39.5	36.0	41.0	31.5	41.0
60-61	37.367473065356876	39.0	35.5	41.0	31.0	41.0
62-63	37.12638754466104	39.0	35.0	41.0	31.0	41.0
64-65	36.91151426083653	39.0	35.0	41.0	31.0	41.0
66-67	36.63388621157359	38.0	35.0	40.0	31.0	41.0
68-69	36.39529496503005	37.0	35.0	40.0	31.0	41.0
70-71	36.10198670900693	37.0	35.0	39.5	31.0	41.0
72-73	35.777621754533456	36.5	34.0	39.0	30.0	41.0
74-75	35.44553174750723	36.0	34.0	39.0	30.0	41.0
76-77	35.126186825075095	35.5	34.0	38.5	30.0	40.0
78-79	34.78308103819211	35.0	34.0	37.0	30.0	39.0
80-81	34.4860512154044	35.0	34.0	37.0	30.0	39.0
82-83	34.198524537399116	35.0	34.0	36.0	29.0	38.5
84-85	33.993093729634495	35.0	34.0	36.0	29.0	37.0
86-87	33.78608031060227	35.0	34.0	36.0	29.0	37.0
88-89	33.56584784773281	35.0	33.0	35.0	29.0	36.5
90-91	33.40879922361331	35.0	33.0	35.0	29.0	36.0
92-93	33.33028290280699	35.0	33.0	35.0	29.0	36.0
94-95	33.3224594969021	35.0	33.0	35.0	29.0	36.0
96-97	33.29453782148623	35.0	33.0	35.0	29.0	36.0
98-99	33.342827850349245	35.0	33.0	35.0	29.0	35.0
100	32.73695863085649	34.0	32.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	1.0
15	10.0
16	47.0
17	277.0
18	1297.0
19	4223.0
20	9623.0
21	17985.0
22	28544.0
23	39723.0
24	52166.0
25	65951.0
26	77607.0
27	83885.0
28	96222.0
29	115240.0
30	136059.0
31	165078.0
32	213354.0
33	284071.0
34	396622.0
35	581376.0
36	925291.0
37	1586960.0
38	2188180.0
39	1416262.0
40	87783.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.892913793586075	20.556425125731295	14.647054295391563	23.90360678529107
2	14.391143567059794	21.22282834087472	41.29974361962611	23.086284472439374
3	17.661357150722566	26.20454452135969	30.61602858534637	25.518069742571374
4	12.495984689448825	18.44661077463243	38.924228028115685	30.133176507803057
5	13.499083288366757	38.14696174073263	35.39248765372912	12.961467317171493
6	29.77627250234104	38.02373654921879	19.046803096379982	13.153187852060185
7	26.413766880267612	33.11643156566305	23.10229517986568	17.36750637420366
8	24.50035523736283	36.38227809387482	21.478184016803176	17.63918265195918
9	25.273680899181507	17.597038840030386	21.275825673300794	35.85345458748731
10-11	23.83209359475627	27.145664709612376	29.16371110120593	19.858530594425424
12-13	24.494837210159524	24.766612411551602	28.90052440908621	21.83802596920266
14-15	21.98554811757647	26.307506832749045	26.283724013858656	25.42322103581583
16-17	21.87270095172689	29.195970313478576	27.03444868417822	21.896880050616314
18-19	21.970486719116913	28.81898762502997	28.061574677334633	21.148950978518485
20-21	22.929549926475012	27.927621139135795	27.691516095702628	21.45131283868657
22-23	22.675033598261383	27.75100181623903	27.517072424969335	22.056892160530257
24-25	22.394645875618043	27.929142804644368	27.54794851431242	22.128262805425166
26-27	22.280143858786275	28.091465205213613	27.508373615092385	22.120017320907728
28-29	22.243835993637536	28.156460452813164	27.515292477231917	22.084411076317384
30-31	22.579634580027236	27.920474739259998	27.43636995149793	22.063520729214833
32-33	22.311753922674296	28.023223296707332	27.424064409109235	22.240958371509137
34-35	22.51530787940858	28.0272326167844	27.406442878703945	22.05101662510307
36-37	22.475220020720144	27.86349796556346	27.463870557132136	22.19741145658426
38-39	22.23469567330858	27.983566101782603	27.61172379587768	22.170014429031138
40-41	22.481861307597857	27.839791896723476	27.54976949272956	22.128577302949104
42-43	22.418689611782977	27.882079041508494	27.409677919378026	22.289553427330503
44-45	22.422309533544478	27.90310487161369	27.39129000475582	22.283295590086013
46-47	22.457050736777198	27.865321015948957	27.328243187859925	22.34938505941392
48-49	22.276867071949823	27.892536337293787	27.50914112146507	22.321455469291323
50-51	22.248517507019415	27.946412213046944	27.48773400777574	22.3173362721579
52-53	22.37328978451191	27.832677573101964	27.434312067497363	22.35972057488876
54-55	22.408981922909106	27.734214256982725	27.471036816224974	22.385767003883196
56-57	22.380380927717646	27.813023584508294	27.4347427149277	22.371852772846353
58-59	22.360691168556595	27.886932170554353	27.44519174574848	22.307184915140567
60-61	22.356405489728687	27.80311443278527	27.5167812885244	22.32369878896164
62-63	22.37341036882553	27.879566372414356	27.43017774098887	22.316845517771245
64-65	22.329551065237503	27.84656570265171	27.426349410179974	22.397533821930814
66-67	22.39630922152375	27.84997886347703	27.308174628433946	22.445537286565273
68-69	22.33672211331642	27.776020783762796	27.426918518038235	22.460338584882543
70-71	22.304482001428223	27.592514081521408	27.495327287094018	22.607676629956348
72-73	22.366973172931527	27.61494840378073	27.501559050147094	22.516519373140653
74-75	22.18753802028528	27.820984296566813	27.445215790691318	22.546261892456588
76-77	22.342154838529535	27.721514111292052	27.412477944653297	22.523853105525117
78-79	22.35462407944536	27.770850199549812	27.361890134404238	22.512635586600588
80-81	22.349687620644225	27.76043755642582	27.36211820092324	22.52775662200671
82-83	22.282496602330554	27.76839608371565	27.451143173723345	22.497964140230447
84-85	22.440499273090765	27.737466029932285	27.443380645520122	22.37865405145683
86-87	22.250211983977692	27.78266996734414	27.499736810930393	22.467381237747773
88-89	22.30748382653505	27.774291110147786	27.459828037392732	22.45839702592443
90-91	22.31359404677587	27.854215593379354	27.494069507829046	22.338120852015734
92-93	22.27899675664739	27.84426022956123	27.486832738860567	22.38991027493081
94-95	22.144163467204574	27.896013667896387	27.59049380240402	22.369329062495023
96-97	22.241389674556178	28.053100579868367	27.58896162627152	22.116548119303935
98-99	23.331055408508643	30.000213111470085	27.73405203992667	18.934679440094605
100	22.293621779544967	30.945968134997564	21.491086755262117	25.269323330195352
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3236.0
1	2583.5
2	2106.5
3	2226.5
4	2165.0
5	2330.0
6	2770.0
7	3187.0
8	3487.0
9	3889.5
10	4364.5
11	4754.5
12	5148.5
13	5735.0
14	6609.0
15	7939.0
16	9509.5
17	11154.5
18	13234.0
19	15929.0
20	19421.5
21	23743.0
22	29421.5
23	37235.5
24	47630.0
25	61326.0
26	78539.5
27	97495.0
28	116716.5
29	137923.0
30	158880.5
31	179104.5
32	201030.5
33	221757.5
34	241759.0
35	260492.0
36	280094.5
37	298399.0
38	311218.0
39	322196.0
40	334615.5
41	346030.0
42	353916.5
43	360819.0
44	365287.5
45	369205.5
46	370970.5
47	369557.5
48	367991.0
49	362609.0
50	354459.5
51	344622.0
52	331294.0
53	314661.0
54	297062.0
55	278253.0
56	257661.5
57	234752.5
58	210803.0
59	186667.5
60	159860.5
61	133071.5
62	108495.0
63	86651.5
64	67910.5
65	52526.0
66	40260.0
67	31730.0
68	25356.5
69	19776.0
70	15416.0
71	12006.5
72	9281.5
73	7058.0
74	5379.5
75	4248.0
76	3340.5
77	2585.5
78	1997.0
79	1543.0
80	1194.5
81	915.5
82	722.0
83	508.0
84	351.5
85	267.5
86	223.5
87	190.5
88	128.0
89	75.5
90	50.5
91	40.0
92	29.5
93	20.0
94	16.0
95	13.5
96	14.0
97	13.5
98	8.0
99	11.5
100	36.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.02112239090069327
3	0.012316534948167914
4	0.0047353344592388005
5	0.009400688606272103
6	0.00853759808907094
7	0.002565944780868316
8	0.008152706371940694
9	0.005166879717839381
10-11	0.003796431937148349
12-13	0.008887499650098439
14-15	0.004945275395855299
16-17	0.0037264516249428496
18-19	0.005196038181258339
20-21	0.004171911702976691
22-23	0.010078170635690832
24-25	0.009233137121377827
26-27	0.021965181180864518
28-29	0.01864452815900719
30-31	0.01546787141132909
32-33	0.011583919964358981
34-35	0.008444279317127452
36-37	0.010083167103762449
38-39	0.004957731447471368
40-41	0.00594211081029217
42-43	0.003805129703863653
44-45	0.007425053405509022
46-47	0.009229757257384131
48-49	0.00711213728099867
50-51	0.009620326406004395
52-53	8.277722426699652E-4
54-55	0.002372881399529308
56-57	6.370075103185466E-4
58-59	0.0026289078386407916
60-61	9.236467727549187E-4
62-63	0.006481598774662147
64-65	0.0033616716594848006
66-67	0.0036835503157510462
68-69	0.004883478260869565
70-71	7.32416854489335E-4
72-73	5.061362605783369E-4
74-75	0.0
76-77	1.4618894555740929E-5
78-79	4.519039044867761E-4
80-81	2.0279455401984997E-4
82-83	0.0
84-85	9.501486364633212E-4
86-87	0.003501214473957833
88-89	0.021706083812795807
90-91	0.0027368566721966874
92-93	0.0012479681260675912
94-95	0.00127277251323852
96-97	4.5906973243250575E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3173.0
20-21	5915.0
22-23	6772.0
24-25	8042.0
26-27	10468.0
28-29	15503.0
30-31	26344.0
32-33	32508.0
34-35	41499.0
36-37	57154.0
38-39	62928.0
40-41	61868.0
42-43	67852.0
44-45	77178.0
46-47	77441.0
48-49	74986.0
50-51	73297.0
52-53	75346.0
54-55	82333.0
56-57	84410.0
58-59	83669.0
60-61	80901.0
62-63	80912.0
64-65	85483.0
66-67	88602.0
68-69	87560.0
70-71	86685.0
72-73	84651.0
74-75	87451.0
76-77	90626.0
78-79	91816.0
80-81	92596.0
82-83	91908.0
84-85	95094.0
86-87	114295.0
88-89	103559.0
90-91	105533.0
92-93	114153.0
94-95	138677.0
96-97	307172.0
98-99	517449.0
100-101	5000031.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.04807252756005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.97821387076552	79.19478159450266
2	6.36288591967554	11.077539100410142
3	1.5142641548662958	3.9544132793605726
4	0.5454123064887381	1.8990836005062195
5	0.246576067648453	1.0731985710111545
6	0.12332854526853554	0.6441307291952372
7	0.06879293195068882	0.4191804490878945
8	0.04614375171803007	0.3213379716995844
9	0.02898695376710722	0.22709326084849615
>10	0.08420697816211718	1.070980765831493
>50	8.805900222988897E-4	0.047122345155475594
>100	2.679577885357352E-4	0.038171107945707765
>500	1.9987009783440083E-5	0.009012322908092554
>1k	1.9984868304196035E-5	0.023954901537276092
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.499015610274976E-5
2	0.0	3.499015610274976E-5	0.0	0.0	3.499015610274976E-5
3	0.0	3.499015610274976E-5	0.0	0.0	3.499015610274976E-5
4	0.0	5.8316926837916266E-5	0.0	0.0	4.665354147033301E-5
5	0.0	5.8316926837916266E-5	0.0	1.1663385367583252E-5	4.665354147033301E-5
6	0.0	5.8316926837916266E-5	0.0	2.3326770735166505E-5	6.998031220549952E-5
7	0.0	5.8316926837916266E-5	0.0	3.499015610274976E-5	6.998031220549952E-5
8	0.0	5.8316926837916266E-5	0.0	3.499015610274976E-5	8.164369757308277E-5
9	0.0	9.330708294066602E-5	0.0	4.665354147033301E-5	9.330708294066602E-5
10-11	0.0	9.330708294066602E-5	0.0	5.2485234154124634E-5	9.330708294066602E-5
12-13	0.0	1.1663385367583253E-4	0.0	5.8316926837916266E-5	9.913877562445764E-5
14-15	0.0	1.6328739514616554E-4	0.0	6.41486195217079E-5	1.282972390434158E-4
16-17	0.0	1.8078247319754041E-4	0.0	6.998031220549952E-5	1.5745570246237392E-4
18-19	0.0	2.2160432198408181E-4	0.0	6.998031220549952E-5	1.6911908782995717E-4
20-21	0.0	2.4493109271924834E-4	0.0	8.164369757308277E-5	1.924458585651237E-4
22-23	0.0	2.857529415057897E-4	0.0	1.1080216099204091E-4	1.9827755124891532E-4
24-25	5.831692683791626E-6	3.5573325371128923E-4	0.0	1.8078247319754044E-4	1.9827755124891532E-4
26-27	1.1663385367583252E-5	3.848917171302474E-4	0.0	4.082184878654139E-4	2.2743601466787344E-4
28-29	1.1663385367583252E-5	3.90723409814039E-4	0.0	0.0016270422587778637	2.3326770735166506E-4
30-31	1.1663385367583252E-5	4.898621854384967E-4	0.0	0.006274901327759791	2.3326770735166506E-4
32-33	1.1663385367583252E-5	5.073572634898716E-4	0.0	0.01756505836358038	2.4493109271924834E-4
34-35	1.1663385367583252E-5	6.531495805846622E-4	0.0	0.030004058858107918	2.4493109271924834E-4
36-37	1.1663385367583252E-5	8.047735903632446E-4	0.0	0.04602371866048352	2.4493109271924834E-4
38-39	1.1663385367583252E-5	8.339320537822026E-4	0.0	0.06300560775568473	2.4493109271924834E-4
40-41	1.1663385367583252E-5	9.505659074580352E-4	0.0	0.07892612878243588	2.5076278540304E-4
42-43	1.1663385367583252E-5	9.6806098550941E-4	0.0	0.09560476985807992	2.565944780868316E-4
44-45	1.1663385367583252E-5	0.0011196849952879923	0.0	0.1121317869239454	2.6825786345441484E-4
46-47	1.1663385367583252E-5	0.0012538139270151997	0.0	0.1292011514094035	2.9158463418958134E-4
48-49	1.1663385367583252E-5	0.0012596456196989913	0.0	0.1435587787968985	3.324064829761227E-4
50-51	1.1663385367583252E-5	0.0014054379367937821	0.0	0.15756650462336597	3.3823817565991433E-4
52-53	1.1663385367583252E-5	0.0014171013221613653	0.0	0.1714809233668928	3.7906002444645573E-4
54-55	1.1663385367583252E-5	0.0014404280928965317	0.0	0.18471886575909977	4.257135659167888E-4
56-57	1.1663385367583252E-5	0.0014579231709479067	0.0	0.1978051841415282	4.37376951284372E-4
58-59	1.1663385367583252E-5	0.00146958655631549	0.0	0.21176625642652536	4.548720293357469E-4
60-61	1.1663385367583252E-5	0.00146958655631549	0.0	0.22307974023308108	4.898621854384967E-4
62-63	1.1663385367583252E-5	0.0014929133270506563	0.0	0.23349514336633292	4.898621854384967E-4
64-65	1.1663385367583252E-5	0.0016678641075644053	0.0	0.24252843533352617	4.898621854384967E-4
66-67	1.1663385367583252E-5	0.001673695800248197	0.0	0.2508619241786644	5.131889561736632E-4
68-69	1.1663385367583252E-5	0.0017145176490347383	0.0	0.25838480774075556	5.248523415412464E-4
70-71	1.1663385367583252E-5	0.0017436761124536963	0.0	0.26500961062954287	5.598424976439962E-4
72-73	1.1663385367583252E-5	0.001761171190505071	0.0	0.2713253338060892	6.064960391143292E-4
74-75	1.1663385367583252E-5	0.0017961613466078211	0.0	0.2771920166459836	6.064960391143292E-4
76-77	1.1663385367583252E-5	0.001860309966129529	0.0	0.281180894441697	6.064960391143292E-4
78-79	1.1663385367583252E-5	0.0019011318149160703	0.0	0.28358355182741923	6.123277317981208E-4
80-81	1.1663385367583252E-5	0.0019011318149160703	0.0	0.28484319744711817	6.589812732684538E-4
82-83	1.1663385367583252E-5	0.001918626892967445	0.0	0.2859803775204576	6.998031220549952E-4
84-85	1.1663385367583252E-5	0.0019594487417539867	0.0	0.28644108124247714	6.998031220549952E-4
86-87	1.1663385367583252E-5	0.001982775512489153	0.0	0.28652855663273397	7.172982001063701E-4
88	1.1663385367583252E-5	0.0020994093661649855	0.0	0.28654605171078534	7.581200488929115E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7095	0.0	38.447372	1
GTATCAA	11380	0.0	30.819197	1
TCAACGC	12690	0.0	26.228052	4
ATCAACG	12895	0.0	25.909634	3
CAACGCA	13270	0.0	25.143862	5
AACGCAG	13815	0.0	24.212793	6
TATCAAC	14140	0.0	23.983345	2
ACGCAGA	16650	0.0	19.941986	7
GTACATG	26850	0.0	19.701513	1
CGCAGAG	16935	0.0	19.581675	8
TACATGG	26765	0.0	19.098606	2
ACATGGG	26965	0.0	18.201153	3
CATGGGG	19815	0.0	16.7134	4
ACCGAAC	1125	0.0	15.84386	8
GGACCGA	1435	0.0	15.597826	6
GCAGAGT	21120	0.0	15.328499	9
AGAGTAC	17780	0.0	15.142012	10-11
AGGACCG	1895	0.0	14.654554	5
CAGAGTA	20280	0.0	14.511977	10-11
GTATAAT	5065	0.0	14.241737	1
>>END_MODULE