FastQCFastQC Report
Fri 27 May 2016
SRR2138840_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138840_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9429237
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78700.034.584451
GTATCAA123700.030.2031541
TCAACGC128750.027.5688614
ATCAACG131300.027.0989553
CAACGCA135350.026.2243965
AACGCAG138500.025.5974466
TATCAAC146500.024.72382
ACGCAGA167800.021.103977
CGCAGAG170550.020.7632438
GTACATG276850.019.0061681
TACATGG270050.018.7492832
ACATGGG275550.017.6974473
GCAGAGT224050.015.8808849
CATGGGG204800.015.7015114
AGAGTAC184900.015.56403810-11
CAGAGTA214600.014.47337210-11
GTGTAGC55200.014.1645361
GTACTAG22450.013.3663161
TATACCG6650.013.343885
GTATAAG40300.013.3189011
Produced by FastQC (version 0.11.5)