##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138840_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9429237
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00292388450943	33.0	31.0	34.0	30.0	34.0
2	32.23328812288842	34.0	31.0	34.0	30.0	34.0
3	32.33994489692008	34.0	31.0	34.0	30.0	34.0
4	35.72149390242286	37.0	35.0	37.0	35.0	37.0
5	35.825309619431565	37.0	35.0	37.0	35.0	37.0
6	35.86307662009132	37.0	36.0	37.0	35.0	37.0
7	35.7963138480876	37.0	36.0	37.0	35.0	37.0
8	35.735801316691905	37.0	36.0	37.0	35.0	37.0
9	37.47512327879764	39.0	38.0	39.0	35.0	39.0
10-11	37.47201242263823	39.0	38.0	39.0	35.0	39.0
12-13	37.406537877879195	39.0	38.0	39.0	35.0	39.0
14-15	38.82478529280789	41.0	39.0	41.0	35.0	41.0
16-17	38.82830302176093	41.0	39.0	41.0	35.0	41.0
18-19	38.84163925458656	41.0	39.0	41.0	35.0	41.0
20-21	38.87959003852691	41.0	39.0	41.0	34.5	41.0
22-23	38.83860811627909	40.5	39.0	41.0	34.0	41.0
24-25	38.80533041422336	40.5	38.5	41.0	34.0	41.0
26-27	38.76048671761441	40.0	38.0	41.0	34.0	41.0
28-29	38.68620776678229	40.0	38.0	41.0	34.0	41.0
30-31	38.56418632903405	40.0	38.0	41.0	34.0	41.0
32-33	38.5100512638482	40.0	38.0	41.0	34.0	41.0
34-35	38.35396595228003	40.0	38.0	41.0	33.0	41.0
36-37	38.23802512524496	40.0	38.0	41.0	33.0	41.0
38-39	38.22524999754705	40.0	38.0	41.0	33.0	41.0
40-41	38.141612571088665	40.0	38.0	41.0	33.0	41.0
42-43	37.96101683359336	40.0	38.0	41.0	33.0	41.0
44-45	38.021212099633075	40.0	37.5	41.0	33.0	41.0
46-47	38.234142380156655	40.0	38.0	41.0	33.0	41.0
48-49	38.22876824269742	40.0	38.0	41.0	33.0	41.0
50-51	38.13649030174787	40.0	38.0	41.0	33.0	41.0
52-53	38.06187219641943	40.0	37.0	41.0	33.0	41.0
54-55	37.98206967288799	40.0	37.0	41.0	33.0	41.0
56-57	38.030099483449376	40.0	37.0	41.0	33.0	41.0
58-59	37.834943259450675	40.0	36.5	41.0	32.5	41.0
60-61	37.74933906733561	40.0	36.0	41.0	32.0	41.0
62-63	37.528993668300146	39.0	36.0	41.0	32.0	41.0
64-65	37.31551559753227	39.0	35.0	41.0	32.0	41.0
66-67	37.033400616966674	39.0	35.0	41.0	31.5	41.0
68-69	36.79237844099774	38.0	35.0	40.0	31.0	41.0
70-71	36.49640386547411	37.0	35.0	40.0	31.0	41.0
72-73	36.165640251176626	37.0	35.0	39.0	31.0	41.0
74-75	35.82508632373148	36.0	35.0	39.0	31.0	41.0
76-77	35.493207051850604	36.0	34.5	39.0	31.0	40.5
78-79	35.1484927727643	35.5	34.0	37.5	30.5	39.5
80-81	34.83301215287306	35.0	34.0	37.0	30.0	39.0
82-83	34.529125302202345	35.0	34.0	37.0	30.0	39.0
84-85	34.3105029745008	35.0	34.0	36.0	30.0	37.5
86-87	34.09702695228454	35.0	34.0	36.0	30.0	37.0
88-89	33.87040347974134	35.0	34.0	35.5	30.0	37.0
90-91	33.70504192462723	35.0	34.0	35.0	30.0	36.0
92-93	33.61408076402489	35.0	34.0	35.0	30.0	36.0
94-95	33.58795902890006	35.0	34.0	35.0	30.0	36.0
96-97	33.544296939308275	35.0	34.0	35.0	30.0	36.0
98-99	33.58007207010711	35.0	34.0	35.0	30.0	36.0
100	32.99554411672952	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	1.0
15	14.0
16	39.0
17	272.0
18	1421.0
19	4221.0
20	9153.0
21	16914.0
22	25741.0
23	36368.0
24	47552.0
25	60694.0
26	72209.0
27	78802.0
28	90887.0
29	111217.0
30	130401.0
31	157370.0
32	203145.0
33	270163.0
34	382288.0
35	568188.0
36	925916.0
37	1704753.0
38	2621162.0
39	1790010.0
40	120333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.855203872805404	20.558810856063964	14.748828563753355	23.83715670737728
2	14.141208525494347	21.141744315150817	41.94372734151271	22.773319817842125
3	17.77213126543136	26.18193682208883	30.558837137127732	25.48709477535208
4	12.284268287858572	18.50191058591549	39.531878080129744	29.681943046096194
5	13.416761629036678	38.0874020077435	35.59951872533488	12.896317637884938
6	29.408942184791915	38.2423276480434	19.434203187041827	12.914526980122856
7	26.34921065880265	32.98621403744366	23.299408342762636	17.36516696099105
8	24.192541988898416	36.825384198884535	21.614794050094474	17.36727976212257
9	25.005273769907088	18.014402562209007	21.572142361772872	35.40818130611103
10-11	23.67589731798361	27.354416369459095	29.291312135189813	19.678374177367484
12-13	24.3873164531977	24.783103891378612	29.176507260840484	21.653072394583205
14-15	21.814142913959962	26.404019620840515	26.529471032745594	25.252366432453933
16-17	21.67986873952388	29.4124263148598	27.215263140060404	21.692441805555916
18-19	21.74774867839457	28.980672170156513	28.305630987314878	20.96594816413404
20-21	22.667459999347436	28.068501076065765	28.003647986299363	21.260390938287436
22-23	22.355006217718525	27.988886203088846	27.776279145008093	21.879828434184535
24-25	22.08833493661252	28.14189815044785	27.84733112853447	21.922435784405163
26-27	22.116218860072106	28.261024593400442	27.742583066175612	21.880173480351843
28-29	22.02389483105621	28.321639826649186	27.786003610375072	21.868461731919535
30-31	22.35680747267853	28.086407111912052	27.74216225659942	21.81462315880999
32-33	22.111845142512376	28.214052859349774	27.71356540107511	21.96053659706274
34-35	22.270634149041584	28.242030241524347	27.69119305202496	21.796142557409112
36-37	22.24807641016881	28.08591721233315	27.755697209498486	21.910309167999554
38-39	22.00306005348185	28.20414675880819	27.875979674707978	21.916813513001976
40-41	22.27451439222624	28.070687169491126	27.79445177174536	21.86034666653727
42-43	22.17904545133106	28.11554818555611	27.70817808613957	21.997228276973267
44-45	22.172712736348394	28.135417505869032	27.688307064020073	22.0035626937625
46-47	22.192499400147174	28.078420202642203	27.619229243142797	22.109851154067826
48-49	22.024166672234266	28.123887474469285	27.80100979210549	22.05093606119096
50-51	22.021996783964852	28.16426963782903	27.777384446480713	22.036349131725405
52-53	22.13290212666538	28.062727724794655	27.705663460528196	22.098706688011767
54-55	22.165679005784238	27.966344640097663	27.74960682802622	22.118369526091882
56-57	22.104449146467502	28.075972640246828	27.76174363923815	22.05783457404752
58-59	22.14323328375929	28.1570525364552	27.727378933015984	21.97233524676952
60-61	22.160180319604574	28.060322282496458	27.786089765684345	21.993407632214623
62-63	22.181676543582043	28.084294286810103	27.71156919835065	22.022459971257206
64-65	22.13775439371166	28.071699522088352	27.69693609172973	22.093609992470263
66-67	22.201129015078894	28.09896380152463	27.619646688273154	22.080260495123323
68-69	22.12523996911421	28.06668304642616	27.690278633239245	22.11779835122038
70-71	22.104035360639458	27.861692721382948	27.78308079092713	22.251191127050465
72-73	22.138596909230873	27.920516333102523	27.736004894347875	22.20488186331873
74-75	21.979658904654606	28.043571925785717	27.744483708912178	22.232285460647503
76-77	22.127160028546456	27.992629240872752	27.67058031096392	22.209630419616868
78-79	22.096942134288707	28.0209904808543	27.654168307277864	22.227899077579128
80-81	22.117649020532447	28.013886072418515	27.65195695582767	22.216507951221367
82-83	22.101405781035265	28.035788227961483	27.75564711640136	22.107158874601893
84-85	22.208581599061546	27.98857549069772	27.734580230158794	22.06826268008194
86-87	22.01084244709655	28.030704588646856	27.840160816680438	22.118292147576163
88-89	22.111899153890306	28.038354095174938	27.744938114311175	22.10480863662358
90-91	22.107007322678925	28.081765916413694	27.810292153803612	22.000934607103773
92-93	22.09938957673604	28.069866314578533	27.790315910395506	22.04042819828992
94-95	21.953750158149926	28.17111952196887	27.833909138995615	22.041221180885586
96-97	22.041046564817833	28.286686792153017	27.871728336172897	21.800538306856254
98-99	23.08935980990769	30.19571685642929	28.118449825430865	18.596473508232155
100	22.09186463839258	31.204783452743413	21.852460791712364	24.85089111715165
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2782.0
1	2203.0
2	1772.0
3	1912.0
4	1883.0
5	2020.0
6	2389.0
7	2834.5
8	3248.0
9	3634.0
10	4049.5
11	4388.0
12	4769.5
13	5347.5
14	6252.0
15	7549.5
16	9019.0
17	10800.0
18	13109.5
19	16044.5
20	19716.0
21	24515.0
22	30959.5
23	39567.5
24	51043.5
25	66112.0
26	84986.0
27	106097.0
28	128671.5
29	153848.5
30	177202.5
31	200309.5
32	225135.0
33	248002.5
34	271460.0
35	293246.0
36	315034.5
37	335175.0
38	348095.0
39	358473.0
40	372021.5
41	383276.0
42	390844.5
43	398528.5
44	402951.0
45	404809.5
46	404162.0
47	401485.5
48	396445.0
49	388129.0
50	378431.5
51	364421.5
52	346338.0
53	327155.0
54	306688.5
55	284724.5
56	261975.0
57	237976.0
58	212505.0
59	186013.5
60	157297.0
61	128819.5
62	102867.5
63	80764.0
64	62448.5
65	47768.0
66	36828.5
67	28917.0
68	22843.0
69	17736.5
70	13852.5
71	10835.0
72	8398.5
73	6394.0
74	4892.0
75	3813.5
76	2922.0
77	2250.0
78	1728.5
79	1320.5
80	1030.0
81	800.5
82	621.0
83	428.0
84	299.0
85	232.5
86	193.0
87	160.5
88	113.5
89	75.5
90	51.5
91	41.5
92	32.0
93	16.0
94	10.5
95	9.5
96	7.5
97	6.5
98	5.0
99	14.0
100	39.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.02152878329391869
3	0.012673347801099919
4	0.004814811633221225
5	0.009979598561368222
6	0.00922662141167944
7	0.003160382966299394
8	0.009035725796265381
9	0.00528144535978892
10-11	0.0038285176202485947
12-13	0.008669842533388438
14-15	0.005164786928146996
16-17	0.0037277671565578425
18-19	0.00586473751799854
20-21	0.004573037213435159
22-23	0.010367540732278566
24-25	0.00963627830357942
26-27	0.02337200009931505
28-29	0.02002405228268341
30-31	0.016289647094793427
32-33	0.013102316314185312
34-35	0.00938007064818219
36-37	0.010956033502193065
38-39	0.005542835990137978
40-41	0.0064166936527614675
42-43	0.004004744030750729
44-45	0.007481119290369977
46-47	0.0100522331857465
48-49	0.007578845678926751
50-51	0.010576380480789016
52-53	9.753733070214501E-4
54-55	0.0030426690914457802
56-57	6.50080708680841E-4
58-59	0.0030406727772667353
60-61	0.0010290488666919238
62-63	0.007556724228093454
64-65	0.004031042036417362
66-67	0.004181137083052537
68-69	0.005387195116888912
70-71	9.757255638233279E-4
72-73	5.275136685699444E-4
74-75	0.0
76-77	1.2829866286492068E-5
78-79	4.736096895611753E-4
80-81	1.838133323092305E-4
82-83	0.0
84-85	9.613555247354088E-4
86-87	0.003844401528926668
88-89	0.022338785034286577
90-91	0.0028477910400357335
92-93	0.001309919285478897
94-95	0.001353484473397914
96-97	5.336199860843765E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3011.0
20-21	5770.0
22-23	6378.0
24-25	7456.0
26-27	9816.0
28-29	14001.0
30-31	23027.0
32-33	28230.0
34-35	36915.0
36-37	52050.0
38-39	57315.0
40-41	56533.0
42-43	62025.0
44-45	70524.0
46-47	72025.0
48-49	70993.0
50-51	69626.0
52-53	71345.0
54-55	77876.0
56-57	80042.0
58-59	80123.0
60-61	78187.0
62-63	77912.0
64-65	81234.0
66-67	85255.0
68-69	85109.0
70-71	84151.0
72-73	81961.0
74-75	84449.0
76-77	86968.0
78-79	89622.0
80-81	90931.0
82-83	88502.0
84-85	92410.0
86-87	113305.0
88-89	100052.0
90-91	104062.0
92-93	111096.0
94-95	138415.0
96-97	329129.0
98-99	589295.0
100-101	5882111.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.95690523973929
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.44303308046621	77.74203224092238
2	6.5838647263458006	11.318572727875358
3	1.6573886978569652	4.273920097413182
4	0.6102398325216082	2.09817309830297
5	0.2832948481649673	1.217557420931121
6	0.1512551153844299	0.7800852972075164
7	0.08210986595315034	0.4940536976887847
8	0.051762439613997506	0.3559471293502488
9	0.03595774704473647	0.2781734989823035
>10	0.09922003658685949	1.2767934411196171
>50	0.001410012887565686	0.0780968420350578
>100	4.252384963105391E-4	0.060761321423391274
>500	1.9180172764727074E-5	0.009216055756860854
>1k	1.9178504569487275E-5	0.01661713099118679
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.060531196744763E-5	1.060531196744763E-5
2	0.0	1.060531196744763E-5	0.0	2.121062393489526E-5	1.060531196744763E-5
3	0.0	1.060531196744763E-5	0.0	2.121062393489526E-5	1.060531196744763E-5
4	0.0	1.0605311967447631E-4	0.0	2.121062393489526E-5	1.060531196744763E-5
5	0.0	1.0605311967447631E-4	0.0	8.484249573958104E-5	1.060531196744763E-5
6	3.1815935902342895E-5	1.0605311967447631E-4	0.0	8.484249573958104E-5	5.3026559837238156E-5
7	3.1815935902342895E-5	1.0605311967447631E-4	0.0	8.484249573958104E-5	5.3026559837238156E-5
8	3.1815935902342895E-5	1.1665843164192394E-4	0.0	8.484249573958104E-5	6.363187180468579E-5
9	3.1815935902342895E-5	2.01500927381505E-4	0.0	9.544780770702868E-5	6.363187180468579E-5
10-11	3.1815935902342895E-5	2.01500927381505E-4	0.0	1.007504636907525E-4	6.363187180468579E-5
12-13	3.1815935902342895E-5	2.0680358336522882E-4	0.0	1.1135577565820012E-4	6.89345277884096E-5
14-15	3.1815935902342895E-5	2.4922483123501933E-4	0.0	1.1665843164192394E-4	8.484249573958104E-5
16-17	5.3026559837238156E-5	2.5452748721874316E-4	0.0	1.378690555768192E-4	9.014515172330487E-5
18-19	5.3026559837238156E-5	2.86343423121086E-4	0.0	1.696849914791621E-4	1.325663995930954E-4
20-21	5.3026559837238156E-5	3.287646709908766E-4	0.0	1.9089561541405737E-4	1.378690555768192E-4
22-23	5.3026559837238156E-5	3.711859188606671E-4	0.0	2.01500927381505E-4	1.590796795117145E-4
24-25	5.3026559837238156E-5	4.3481779066535285E-4	0.0	2.651327991861908E-4	1.8559295943033357E-4
26-27	5.3026559837238156E-5	4.454231026328005E-4	0.0	5.46173566323553E-4	2.01500927381505E-4
28-29	5.3026559837238156E-5	4.454231026328005E-4	0.0	0.0022059048892291073	2.01500927381505E-4
30-31	5.3026559837238156E-5	5.620815342747245E-4	0.0	0.008362288486332458	2.01500927381505E-4
32-33	5.8329215820961976E-5	5.620815342747245E-4	0.0	0.023649845687408216	2.1210623934895262E-4
34-35	6.363187180468579E-5	6.469240300143055E-4	0.0	0.03840183463412787	2.1210623934895262E-4
36-37	6.363187180468579E-5	7.317665257538865E-4	0.0	0.05726338196823348	2.1210623934895262E-4
38-39	6.363187180468579E-5	7.423718377213342E-4	0.0	0.07825659700779608	2.1210623934895262E-4
40-41	6.363187180468579E-5	8.590302693632581E-4	0.0	0.09581369096990562	2.1210623934895262E-4
42-43	6.363187180468579E-5	8.802408932981533E-4	0.0	0.11219359530362849	2.1210623934895262E-4
44-45	6.363187180468579E-5	9.703860450214583E-4	0.0	0.12852047307751413	2.1210623934895262E-4
46-47	6.363187180468579E-5	0.0010446232287935919	0.0	0.14609347500757486	2.333168632838479E-4
48-49	6.363187180468579E-5	0.0010499258847773156	0.0	0.15905846888777958	2.8634342312108607E-4
50-51	7.423718377213342E-5	0.0011824922843704107	0.0	0.16990240037449478	2.8634342312108607E-4
52-53	7.423718377213342E-5	0.0011877949403541347	0.0	0.17857754556386696	3.3406732697460035E-4
54-55	7.423718377213342E-5	0.0011877949403541347	0.0	0.18710421638569485	3.711859188606671E-4
56-57	7.423718377213342E-5	0.0011984002523215823	0.0	0.19567861111137624	3.711859188606671E-4
58-59	7.423718377213342E-5	0.001203702908305306	0.0	0.20412574209344828	3.764885748443909E-4
60-61	7.423718377213342E-5	0.0012249135322402013	0.0	0.21074875941711935	3.9239654279556234E-4
62-63	1.1665843164192394E-4	0.001262032124126268	0.0	0.2175626723562044	3.9239654279556234E-4
64-65	1.1665843164192394E-4	0.0014529277395403256	0.0	0.2239682807845428	3.9239654279556234E-4
66-67	1.2726374360937158E-4	0.0014741383634752207	0.0	0.23067614060395342	3.9769919877928616E-4
68-69	1.2726374360937158E-4	0.001495348987410116	0.0	0.23691736669679636	4.0300185476301E-4
70-71	1.2726374360937158E-4	0.0015059542993775636	0.0	0.24302072373406247	4.2421247869790525E-4
72-73	1.378690555768192E-4	0.0015165596113450113	0.0	0.24871047360459814	4.6663372656769575E-4
74-75	1.378690555768192E-4	0.0015430728912636303	0.0	0.25390707646864746	4.6663372656769575E-4
76-77	1.378690555768192E-4	0.001580191483149697	0.0	0.2573060789542144	4.6663372656769575E-4
78-79	1.378690555768192E-4	0.0015907967951171447	0.0	0.2594854705635249	4.6663372656769575E-4
80-81	1.378690555768192E-4	0.0015960994511008686	0.0	0.26069447612781393	4.825416945188672E-4
82-83	1.378690555768192E-4	0.00161200741905204	0.0	0.2615588090531609	4.984496624700387E-4
84-85	1.378690555768192E-4	0.0016332180429869353	0.0	0.26186636310021694	4.984496624700387E-4
86-87	1.378690555768192E-4	0.001643823354954383	0.0	0.2619618109079239	5.037523184537625E-4
88	1.4847436754426683E-4	0.001696849914791621	0.0	0.2619830215318588	5.090549744374863E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7870	0.0	34.58445	1
GTATCAA	12370	0.0	30.203154	1
TCAACGC	12875	0.0	27.568861	4
ATCAACG	13130	0.0	27.098955	3
CAACGCA	13535	0.0	26.224396	5
AACGCAG	13850	0.0	25.597446	6
TATCAAC	14650	0.0	24.7238	2
ACGCAGA	16780	0.0	21.10397	7
CGCAGAG	17055	0.0	20.763243	8
GTACATG	27685	0.0	19.006168	1
TACATGG	27005	0.0	18.749283	2
ACATGGG	27555	0.0	17.697447	3
GCAGAGT	22405	0.0	15.880884	9
CATGGGG	20480	0.0	15.701511	4
AGAGTAC	18490	0.0	15.564038	10-11
CAGAGTA	21460	0.0	14.473372	10-11
GTGTAGC	5520	0.0	14.164536	1
GTACTAG	2245	0.0	13.366316	1
TATACCG	665	0.0	13.34388	5
GTATAAG	4030	0.0	13.318901	1
>>END_MODULE