FastQCFastQC Report
Fri 27 May 2016
SRR2138839_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138839_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11511248
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156800.047.8925551
GTATCAA279800.037.2925721
TCAACGC339250.030.0897394
ATCAACG339550.030.0884023
CAACGCA347450.029.4042825
TATCAAC357700.028.9555472
AACGCAG356950.028.6337176
ACGCAGA411850.024.7647677
CGCAGAG416050.024.5147678
CTTATAC84950.022.7688471
GCAGAGT489950.020.6427849
GTACATG338250.019.9569821
TACATGG336750.019.7150062
GAGTACT287450.019.598612-13
GTGGTAT81850.019.1250271
AGAGTAC438250.018.94958710-11
ACATGGG338450.018.8109533
TATACAC136750.018.6852023
CAGAGTA474350.018.32994310-11
GTACTTT316850.017.75328814-15
Produced by FastQC (version 0.11.5)