FastQCFastQC Report
Fri 27 May 2016
SRR2138838_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138838_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10633143
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87050.036.9366761
GTATCAA139600.031.5070151
TCAACGC149000.028.2093414
ATCAACG150750.027.8837033
CAACGCA155400.027.1287335
AACGCAG162700.026.0674296
TATCAAC171000.024.9313132
ACGCAGA194900.021.6316247
CGCAGAG201300.020.8807288
GTACATG323550.019.3921951
TACATGG317950.019.2690052
ACATGGG329950.018.0532633
GCAGAGT259600.016.1263969
CATGGGG237700.015.8345114
AGAGTAC219550.014.84412210-11
CAGAGTA249150.014.46239410-11
GTATAGC41050.014.1009811
GTATAAT71500.013.7095721
GTGGTAT62650.013.0834591
TATACTG57250.012.8387795
Produced by FastQC (version 0.11.5)