##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138838_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10633143
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92789093497567	33.0	31.0	34.0	30.0	34.0
2	32.17993090095751	34.0	31.0	34.0	30.0	34.0
3	32.27899756450186	34.0	31.0	34.0	30.0	34.0
4	35.674710854542255	37.0	35.0	37.0	35.0	37.0
5	35.78329897378414	37.0	35.0	37.0	35.0	37.0
6	35.82028954185982	37.0	36.0	37.0	35.0	37.0
7	35.75020527796908	37.0	36.0	37.0	35.0	37.0
8	35.69039436411229	37.0	36.0	37.0	35.0	37.0
9	37.42097280173886	39.0	38.0	39.0	35.0	39.0
10-11	37.41814118365567	39.0	38.0	39.0	35.0	39.0
12-13	37.34957914136959	39.0	38.0	39.0	34.5	39.0
14-15	38.75179723436429	41.0	39.0	41.0	35.0	41.0
16-17	38.75163340698042	41.0	38.5	41.0	34.5	41.0
18-19	38.76202788770921	40.5	38.5	41.0	34.0	41.0
20-21	38.80349301638498	40.5	39.0	41.0	34.0	41.0
22-23	38.76040332250319	40.0	38.5	41.0	34.0	41.0
24-25	38.72791281936736	40.0	38.0	41.0	34.0	41.0
26-27	38.68338537164537	40.0	38.0	41.0	34.0	41.0
28-29	38.610105041700734	40.0	38.0	41.0	34.0	41.0
30-31	38.489381559398524	40.0	38.0	41.0	34.0	41.0
32-33	38.436741364189686	40.0	38.0	41.0	34.0	41.0
34-35	38.2817212183309	40.0	38.0	41.0	33.0	41.0
36-37	38.16096904504952	40.0	38.0	41.0	33.0	41.0
38-39	38.14833204856911	40.0	38.0	41.0	33.0	41.0
40-41	38.06271275448987	40.0	38.0	41.0	33.0	41.0
42-43	37.87871611547177	40.0	37.5	41.0	32.5	41.0
44-45	37.93564099618454	40.0	37.5	41.0	32.5	41.0
46-47	38.14658262107682	40.0	38.0	41.0	33.0	41.0
48-49	38.13695864635858	40.0	38.0	41.0	33.0	41.0
50-51	38.04044468426477	40.0	37.5	41.0	33.0	41.0
52-53	37.96314752434192	40.0	37.0	41.0	33.0	41.0
54-55	37.88256185145991	40.0	37.0	41.0	33.0	41.0
56-57	37.93521971470146	40.0	37.0	41.0	33.0	41.0
58-59	37.73764939437673	40.0	36.5	41.0	32.0	41.0
60-61	37.64935260422416	39.5	36.0	41.0	32.0	41.0
62-63	37.42035917529204	39.0	35.5	41.0	32.0	41.0
64-65	37.21402545587401	39.0	35.0	41.0	32.0	41.0
66-67	36.9398978925673	38.5	35.0	41.0	31.0	41.0
68-69	36.70406783586989	38.0	35.0	40.0	31.0	41.0
70-71	36.412533200905784	37.0	35.0	40.0	31.0	41.0
72-73	36.08396955549672	37.0	35.0	39.0	31.0	41.0
74-75	35.74751745899742	36.0	34.5	39.0	31.0	41.0
76-77	35.42055205835942	36.0	34.0	39.0	31.0	40.5
78-79	35.073686257510026	35.0	34.0	37.5	30.0	39.5
80-81	34.762109213141294	35.0	34.0	37.0	30.0	39.0
82-83	34.45939047067336	35.0	34.0	36.5	30.0	39.0
84-85	34.23860876663582	35.0	34.0	36.0	30.0	37.5
86-87	34.02113509701282	35.0	34.0	36.0	30.0	37.0
88-89	33.7918359243098	35.0	34.0	35.5	30.0	37.0
90-91	33.62685462615681	35.0	34.0	35.0	29.0	36.0
92-93	33.53577784532223	35.0	34.0	35.0	29.0	36.0
94-95	33.51350904760233	35.0	34.0	35.0	29.5	36.0
96-97	33.4712707264853	35.0	33.0	35.0	29.5	36.0
98-99	33.51054847891052	35.0	33.0	35.0	30.0	35.5
100	32.91515547123624	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	7.0
16	59.0
17	351.0
18	1746.0
19	5233.0
20	11235.0
21	20062.0
22	30472.0
23	42064.0
24	56080.0
25	71654.0
26	85130.0
27	92608.0
28	106674.0
29	128706.0
30	151576.0
31	183250.0
32	235333.0
33	317252.0
34	448358.0
35	663459.0
36	1084454.0
37	1954481.0
38	2897729.0
39	1924270.0
40	120898.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.67435188259953	20.789770249492552	14.792108034284876	23.74376983362304
2	14.14101659380628	21.245050012068667	41.946831264602565	22.667102129522483
3	17.79888005951947	26.376963858005432	30.537768010447884	25.28638807202721
4	12.321874655246958	18.50268010369231	39.52929341626436	29.646151824796373
5	13.38619447292467	38.15414084831332	35.59872699109389	12.860937687668129
6	29.27878710298058	38.3183860319253	19.428344139100616	12.9744827259935
7	26.196388244867126	33.04362072357742	23.397595900096643	17.362395131458815
8	24.12560274880165	36.84837573376684	21.684980348235218	17.3410411691963
9	24.986607151254546	18.018366260554863	21.681696001143656	35.313330587046934
10-11	23.741270905224447	27.389049938355196	29.255633264548603	19.614045891871758
12-13	24.368367595222537	24.8178947050617	29.17087598179601	21.64286171791975
14-15	21.800675544767774	26.416506248140152	26.551407350447736	25.231410856644338
16-17	21.678584029481407	29.419677560868507	27.216161824019792	21.685576585630294
18-19	21.751209674669873	28.973273708012904	28.304880104716595	20.970636512600628
20-21	22.721097883392517	28.098484008035435	27.981339046096842	21.199079062475214
22-23	22.372053342574056	27.99027464156103	27.78059749558837	21.85707452027654
24-25	22.092098170161734	28.129326682711326	27.877004736078035	21.901570411048905
26-27	22.10767561424412	28.27941559754211	27.749014042762845	21.863894745450928
28-29	22.01536684046787	28.34740126830968	27.79306466622945	21.844167224993
30-31	22.363618315108152	28.142186528804686	27.707549765989743	21.78664539009742
32-33	22.11418671423834	28.27091012826764	27.695309305560855	21.919593851933165
34-35	22.231238138572103	28.26247878805287	27.690471919576353	21.815811153798673
36-37	22.24522911331064	28.115986824098815	27.74462003854314	21.89416402404741
38-39	22.011637953620998	28.2245230826586	27.877054956917824	21.886784006802582
40-41	22.26521305835049	28.10218075075647	27.788752619150003	21.843853571743036
42-43	22.171747625503542	28.10037365617019	27.75615676831178	21.97172195001449
44-45	22.19981087487122	28.1314862386687	27.710666175340975	21.958036711119103
46-47	22.189818481018932	28.06151427764959	27.647013176186764	22.101654065144714
48-49	22.02521065942461	28.07183379889992	27.860885758837707	22.04206978283777
50-51	21.996006275909473	28.127227520980647	27.84366488393089	22.033101319178996
52-53	22.12709789067782	28.041942746824915	27.751640200789986	22.079319161707286
54-55	22.162426921284048	27.954069228166293	27.798317860759635	22.085185989790023
56-57	22.11399963079742	28.07997063666453	27.76862831775541	22.037401414782632
58-59	22.090875868658447	28.15196622368003	27.774087203553975	21.983070704107547
60-61	22.133238578703622	28.04994479200567	27.795666611560915	22.02115001772979
62-63	22.10878607229342	28.125893166774006	27.766339950230368	21.998980810702207
64-65	22.08436861121524	28.117604076683158	27.745598617511323	22.052428694590283
66-67	22.172654826512044	28.10743343090481	27.626570505954966	22.093341236628177
68-69	22.074083091870914	28.08889722307855	27.71587678402384	22.121142901026698
70-71	22.06341793986982	27.80730152890222	27.851540757228953	22.27773977399901
72-73	22.12672305911903	27.904558310350296	27.79006331656729	22.17865531396338
74-75	21.92986871625299	28.102348914121716	27.7779767215515	22.189805648073794
76-77	22.080119906087685	28.01797156705035	27.696391164008	22.205517362853968
78-79	22.100622266247022	28.069246916744174	27.61693047952799	22.213200337480814
80-81	22.07494505276741	28.012475105450314	27.697712411634186	22.214867430148093
82-83	22.062475956797776	28.035614114044762	27.78156215789779	22.120347771259677
84-85	22.196738889878205	27.988492012163686	27.75055468348495	22.064214414473163
86-87	21.97113267957747	28.035486432032382	27.841261228417487	22.152119659972662
88-89	22.07037608141089	28.04367861898119	27.75708325399846	22.128862045609463
90-91	22.04644071289275	28.115219759699688	27.816235202016966	22.022104325390597
92-93	22.052690865743312	28.098354137947872	27.80690988856659	22.04204510774223
94-95	21.909947005861792	28.149224896712482	27.866117088282444	22.074711009143286
96-97	21.978316120469284	28.30338372420842	27.922310870554547	21.79598928476775
98-99	23.02318317696613	30.25735802860508	28.11660274319225	18.602856051236543
100	22.031681983474883	31.22508600075354	21.934761581874323	24.808470433897256
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3048.0
1	2338.0
2	1814.5
3	1974.5
4	1933.0
5	2091.0
6	2524.5
7	3023.5
8	3425.5
9	3790.0
10	4153.5
11	4493.5
12	4913.0
13	5602.0
14	6539.5
15	7759.0
16	9354.0
17	11199.5
18	13491.5
19	16625.0
20	20707.0
21	26010.5
22	33485.5
23	43332.5
24	56492.5
25	74308.0
26	97477.5
27	123057.0
28	147874.5
29	174487.0
30	201104.0
31	227488.0
32	255285.0
33	281806.5
34	308506.0
35	332489.5
36	357722.5
37	381615.5
38	396562.5
39	409470.5
40	425290.5
41	438503.0
42	445204.5
43	450938.0
44	453841.0
45	455441.5
46	455882.5
47	451953.0
48	444718.0
49	433127.0
50	420065.0
51	405233.5
52	387036.0
53	366518.5
54	343851.5
55	319041.5
56	292217.5
57	264778.5
58	238280.0
59	210157.0
60	178090.5
61	146333.5
62	117481.5
63	92246.0
64	71048.5
65	54123.5
66	41660.0
67	33221.0
68	26435.0
69	20272.5
70	15727.5
71	12086.5
72	9156.5
73	6935.0
74	5370.0
75	4205.5
76	3243.0
77	2548.5
78	2022.5
79	1576.0
80	1175.5
81	879.5
82	665.0
83	437.5
84	294.5
85	218.0
86	169.0
87	146.0
88	108.0
89	69.5
90	49.0
91	41.0
92	32.0
93	20.0
94	14.0
95	12.5
96	13.5
97	12.5
98	10.0
99	17.0
100	46.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.021715122236200528
3	0.012489251766857645
4	0.0050032243523857435
5	0.009498602623890228
6	0.00951741173799694
7	0.0028871990153804946
8	0.009583243637370438
9	0.005670947903174066
10-11	0.004269668902223924
12-13	0.009122420341755961
14-15	0.00506905625175924
16-17	0.003832356999242839
18-19	0.005976596007408158
20-21	0.004398800007357993
22-23	0.010201733993481718
24-25	0.009422548979469633
26-27	0.022987570255319525
28-29	0.01947270740734709
30-31	0.015448064807161623
32-33	0.012741466310506027
34-35	0.008806426751451824
36-37	0.010381393982449333
38-39	0.005062104189919006
40-41	0.006059716915540303
42-43	0.0039046614899186554
44-45	0.006963880379928157
46-47	0.010113696407959564
48-49	0.007652699975051601
50-51	0.010317326764083565
52-53	9.403978853788454E-4
54-55	0.002956991124897034
56-57	6.22536703297721E-4
58-59	0.0029760320351930073
60-61	0.0010486236840362617
62-63	0.007420011272912989
64-65	0.004060446803017895
66-67	0.003977394583123176
68-69	0.005023169574187388
70-71	9.371462100787428E-4
72-73	5.850074947817332E-4
74-75	0.0
76-77	1.7078690064472054E-5
78-79	3.858554158291829E-4
80-81	1.748516675020691E-4
82-83	0.0
84-85	9.61438149041664E-4
86-87	0.0035001945090789473
88-89	0.02178378061077566
90-91	0.0026817954857773158
92-93	0.0015135619584503812
94-95	0.0013076848451179358
96-97	4.619523505389466E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3563.0
20-21	6856.0
22-23	7779.0
24-25	8868.0
26-27	10952.0
28-29	15040.0
30-31	24782.0
32-33	30806.0
34-35	40599.0
36-37	57060.0
38-39	64384.0
40-41	63439.0
42-43	69725.0
44-45	78987.0
46-47	81493.0
48-49	80382.0
50-51	79095.0
52-53	80650.0
54-55	87409.0
56-57	91475.0
58-59	90973.0
60-61	89500.0
62-63	88105.0
64-65	92039.0
66-67	97422.0
68-69	97297.0
70-71	96705.0
72-73	93719.0
74-75	96579.0
76-77	99732.0
78-79	102968.0
80-81	103730.0
82-83	102214.0
84-85	105658.0
86-87	130007.0
88-89	116244.0
90-91	120617.0
92-93	128621.0
94-95	158993.0
96-97	375034.0
98-99	665444.0
100-101	6598198.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.22876743908743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.25197247717942	77.82316345653624
2	6.829318202637423	11.777673821254979
3	1.6912936090845967	4.375144898669115
4	0.6057216330504264	2.089225193165179
5	0.2686816851491225	1.15840452719329
6	0.1304656149059782	0.6749933499915078
7	0.07616383041157225	0.4597259253880691
8	0.04075070819863003	0.2811106672190226
9	0.026712778513721915	0.20730689695004204
>10	0.07760088331527523	0.9743967638139726
>50	9.619665099112868E-4	0.0558798141690611
>100	2.752350631824334E-4	0.04065761404165887
>500	4.8829926598522834E-5	0.027858760606555993
>1k	3.254605413325181E-5	0.054458311001312466
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.880911410671332E-5	0.0	0.0	0.0
3	0.0	1.880911410671332E-5	0.0	2.821367116006998E-5	0.0
4	0.0	1.1285468464027992E-4	0.0	2.821367116006998E-5	0.0
5	0.0	1.1285468464027992E-4	0.0	4.7022785266783305E-5	0.0
6	3.761822821342664E-5	1.222592416936366E-4	0.0	5.642734232013996E-5	0.0
7	3.761822821342664E-5	1.222592416936366E-4	0.0	7.523645642685329E-5	0.0
8	3.761822821342664E-5	1.410683558003499E-4	0.0	8.464101348020994E-5	0.0
9	3.761822821342664E-5	3.4796861097419646E-4	0.0	1.1285468464027992E-4	9.40455705335666E-6
10-11	3.761822821342664E-5	3.573731680275531E-4	0.0	1.269615202203149E-4	9.40455705335666E-6
12-13	3.761822821342664E-5	3.6677772508090974E-4	0.0	1.5987746990706324E-4	9.40455705335666E-6
14-15	3.761822821342664E-5	4.5612101708779806E-4	0.0	1.6928202696041988E-4	2.821367116006998E-5
16-17	7.523645642685329E-5	4.70227852667833E-4	0.0	2.021979766471682E-4	4.7022785266783305E-5
18-19	7.523645642685329E-5	5.078460808812596E-4	0.0	2.3041164780723816E-4	4.7022785266783305E-5
20-21	7.523645642685329E-5	5.501665876213647E-4	0.0	2.7273215454734316E-4	4.7022785266783305E-5
22-23	7.523645642685329E-5	5.830825373081129E-4	0.0	3.620754465542314E-4	5.642734232013996E-5
24-25	7.523645642685329E-5	6.489144366816095E-4	0.0	4.514187385611197E-4	7.523645642685329E-5
26-27	7.523645642685329E-5	6.771281078416795E-4	0.0	6.583189937349662E-4	7.993873495353162E-5
28-29	7.523645642685329E-5	6.865326648950363E-4	0.0	0.00216775040079871	8.464101348020994E-5
30-31	7.993873495353162E-5	7.711736783752462E-4	0.0	0.007739950454912532	8.464101348020994E-5
32-33	8.464101348020994E-5	7.758759569019245E-4	0.0	0.02024330905735021	8.464101348020994E-5
34-35	8.464101348020994E-5	9.12242034175596E-4	0.0	0.03229054664270009	8.464101348020994E-5
36-37	8.464101348020994E-5	0.0010439058329225893	0.0	0.04808079793528593	8.464101348020994E-5
38-39	8.464101348020994E-5	0.0011050354537694078	0.0	0.06437419303022635	8.464101348020994E-5
40-41	8.464101348020994E-5	0.0012037833028296526	0.0	0.07893244734882246	8.464101348020994E-5
42-43	8.464101348020994E-5	0.0012461038095697575	0.0	0.09371641103669912	8.464101348020994E-5
44-45	8.464101348020994E-5	0.0013918744438967856	0.0	0.1076774759824071	8.464101348020994E-5
46-47	8.464101348020994E-5	0.001495324571483709	0.0	0.12396146652029413	8.464101348020994E-5
48-49	8.464101348020994E-5	0.0015000268500103874	0.0	0.13643661145157174	8.464101348020994E-5
50-51	8.464101348020994E-5	0.0016646065984441288	0.0	0.14702144041512466	8.464101348020994E-5
52-53	8.464101348020994E-5	0.0016740111554974855	0.0	0.15758746026457088	8.934329200688828E-5
54-55	8.464101348020994E-5	0.0016834157125508423	0.0	0.16762212264050244	9.404557053356661E-5
56-57	8.464101348020994E-5	0.0017492476119243389	0.0	0.1773511369121999	9.404557053356661E-5
58-59	8.464101348020994E-5	0.001753949890451017	0.0	0.1862713592773087	9.404557053356661E-5
60-61	8.934329200688828E-5	0.001782163561611087	0.0	0.19461320138363605	1.0345012758692327E-4
62-63	9.404557053356661E-5	0.0018338886254045488	0.0	0.20199577867052104	1.1285468464027992E-4
64-65	9.404557053356661E-5	0.002059597994685109	0.0	0.20872944152072442	1.1285468464027992E-4
66-67	1.0345012758692327E-4	0.002059597994685109	0.0	0.21525150183722724	1.2225924169363656E-4
68-69	1.0345012758692327E-4	0.002116025337005249	0.0	0.2217030279758299	1.3166379874699323E-4
70-71	1.0345012758692327E-4	0.002148941286691997	0.0	0.2276467080335513	1.3166379874699323E-4
72-73	1.1285468464027992E-4	0.002163048122272032	0.0	0.23306843517481143	1.5047291285370657E-4
74-75	1.1285468464027992E-4	0.0022147731860654935	0.0	0.2384854600375449	1.5047291285370657E-4
76-77	1.1285468464027992E-4	0.0023229255921790952	0.0	0.2420827031104538	1.5047291285370657E-4
78-79	1.1285468464027992E-4	0.002360543820392522	0.0	0.24417051477629897	1.5047291285370657E-4
80-81	1.1285468464027992E-4	0.002360543820392522	0.0	0.2452285274448016	1.5987746990706324E-4
82-83	1.1285468464027992E-4	0.002360543820392522	0.0	0.24619249454277065	1.833888625404549E-4
84-85	1.1285468464027992E-4	0.0023699483774458786	0.0	0.24642760846910455	1.8809114106713322E-4
86-87	1.1285468464027992E-4	0.002407566605659305	0.0	0.2465310585966915	1.8809114106713322E-4
88	1.1285468464027992E-4	0.0026050623037797947	0.0	0.24654046315374487	1.8809114106713322E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8705	0.0	36.936676	1
GTATCAA	13960	0.0	31.507015	1
TCAACGC	14900	0.0	28.209341	4
ATCAACG	15075	0.0	27.883703	3
CAACGCA	15540	0.0	27.128733	5
AACGCAG	16270	0.0	26.067429	6
TATCAAC	17100	0.0	24.931313	2
ACGCAGA	19490	0.0	21.631624	7
CGCAGAG	20130	0.0	20.880728	8
GTACATG	32355	0.0	19.392195	1
TACATGG	31795	0.0	19.269005	2
ACATGGG	32995	0.0	18.053263	3
GCAGAGT	25960	0.0	16.126396	9
CATGGGG	23770	0.0	15.834511	4
AGAGTAC	21955	0.0	14.844122	10-11
CAGAGTA	24915	0.0	14.462394	10-11
GTATAGC	4105	0.0	14.100981	1
GTATAAT	7150	0.0	13.709572	1
GTGGTAT	6265	0.0	13.083459	1
TATACTG	5725	0.0	12.838779	5
>>END_MODULE