FastQCFastQC Report
Fri 27 May 2016
SRR2138837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8668014
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114650.047.5917781
GTATCAA207700.038.487011
ATCAACG242000.032.3664553
TCAACGC244300.032.1506844
CAACGCA248600.031.5237085
AACGCAG256800.030.6347966
TATCAAC257650.030.5542642
ACGCAGA288300.027.2274287
CGCAGAG293950.026.6745878
GCAGAGT348500.022.3997129
GAGTACT209750.020.15906312-13
GTACATG232800.019.9354551
TACATGG226600.019.7918322
GTGGTAT57000.019.708351
AGAGTAC311700.019.42305610-11
CAGAGTA344500.019.00888810-11
ACATGGG232100.018.7421133
CTTATAC57400.018.6642441
TGGTATC56250.017.8891962
GTACTTT244200.017.30643714-15
Produced by FastQC (version 0.11.5)