##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138837_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8668014
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.10636611800581	32.0	28.0	33.0	18.0	33.0
2	30.4693048488385	32.0	30.0	33.0	25.0	33.0
3	32.006131162224705	33.0	31.0	33.0	29.0	34.0
4	32.34246010677879	33.0	33.0	33.0	31.0	34.0
5	32.76418600616012	33.0	33.0	34.0	32.0	34.0
6	36.9117614484702	38.0	37.0	38.0	35.0	38.0
7	37.07525218579481	38.0	38.0	38.0	36.0	38.0
8	37.268164772230406	38.0	38.0	38.0	36.0	38.0
9	37.36888807516924	38.0	38.0	38.0	37.0	38.0
10-11	37.42793060786473	38.0	38.0	38.0	37.0	38.0
12-13	37.463470813498915	38.0	38.0	38.0	37.0	38.0
14-15	37.463025094329566	38.0	38.0	38.0	37.0	38.0
16-17	37.396442137726126	38.0	38.0	38.0	37.0	38.0
18-19	37.43138958935691	38.0	38.0	38.0	37.0	38.0
20-21	37.46068714548966	38.0	38.0	38.0	37.0	38.0
22-23	37.46810143087344	38.0	38.0	38.0	37.0	38.0
24-25	37.467584273099135	38.0	38.0	38.0	37.0	38.0
26-27	37.43698871949455	38.0	38.0	38.0	37.0	38.0
28-29	37.41136962329234	38.0	38.0	38.0	37.0	38.0
30-31	37.312254134484604	38.0	38.0	38.0	37.0	38.0
32-33	37.37358196434592	38.0	38.0	38.0	37.0	38.0
34-35	37.38031146739806	38.0	38.0	38.0	37.0	38.0
36-37	37.36059466634421	38.0	38.0	38.0	37.0	38.0
38-39	37.33471890955617	38.0	38.0	38.0	37.0	38.0
40-41	37.32464154025588	38.0	38.0	38.0	37.0	38.0
42-43	37.30087280198842	38.0	38.0	38.0	37.0	38.0
44-45	37.26468536400962	38.0	38.0	38.0	37.0	38.0
46-47	37.23384541854985	38.0	38.0	38.0	37.0	38.0
48-49	37.20281954405853	38.0	38.0	38.0	37.0	38.0
50-51	37.158436304910126	38.0	38.0	38.0	37.0	38.0
52-53	37.13471135897824	38.0	38.0	38.0	36.5	38.0
54-55	37.12122734526385	38.0	38.0	38.0	36.0	38.0
56-57	37.11079855397606	38.0	38.0	38.0	36.0	38.0
58-59	37.099563589886614	38.0	38.0	38.0	36.0	38.0
60-61	37.100449853711496	38.0	38.0	38.0	36.0	38.0
62-63	37.10647986585535	38.0	38.0	38.0	36.0	38.0
64-65	37.1095998878198	38.0	38.0	38.0	36.0	38.0
66-67	37.09100896691161	38.0	38.0	38.0	36.0	38.0
68-69	37.09167020257791	38.0	38.0	38.0	36.0	38.0
70-71	37.07257568046494	38.0	38.0	38.0	36.0	38.0
72-73	37.06228776017922	38.0	38.0	38.0	36.0	38.0
74-75	37.05155496860412	38.0	38.0	38.0	36.0	38.0
76-77	37.05269074852259	38.0	38.0	38.0	36.0	38.0
78-79	37.03413574950284	38.0	38.0	38.0	36.0	38.0
80-81	37.03020079675308	38.0	38.0	38.0	36.0	38.0
82-83	36.97863876403422	38.0	38.0	38.0	36.0	38.0
84-85	36.96530191670061	38.0	38.0	38.0	36.0	38.0
86-87	36.95536267026065	38.0	38.0	38.0	36.0	38.0
88-89	36.95809932443134	38.0	38.0	38.0	36.0	38.0
90-91	36.9496090673739	38.0	38.0	38.0	36.0	38.0
92-93	36.92938430950534	38.0	38.0	38.0	35.5	38.0
94-95	36.90080126451523	38.0	38.0	38.0	35.0	38.0
96-97	36.87109893997679	38.0	38.0	38.0	35.0	38.0
98-99	36.87455057377795	38.0	38.0	38.0	35.0	38.0
100	35.270453863789605	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	12.0
19	26.0
20	72.0
21	219.0
22	733.0
23	1699.0
24	3729.0
25	7530.0
26	13141.0
27	21338.0
28	33490.0
29	48381.0
30	66889.0
31	88083.0
32	115083.0
33	151465.0
34	221631.0
35	393187.0
36	1142710.0
37	6358596.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.654237521997544	19.15492983744604	14.846907261571104	25.343925378985315
2	13.568148854845136	20.684124304299484	43.44510191877718	22.302624922078195
3	17.31936519714897	26.93312447349531	30.999084680758475	24.748425648597244
4	11.827735302522791	18.948306815414213	39.98971579529933	29.23424208676366
5	12.983675053015324	37.80858690757734	36.14802272831892	13.059715311088413
6	28.614120835522417	38.90779364223454	19.46871567120219	13.00936985104085
7	25.54444420601997	33.28861720804789	23.73093767499683	17.436000910935306
8	23.721800633916835	36.89982503489265	21.8240764262725	17.554297904918013
9	24.973024333072566	17.451275075429766	21.99345477392297	35.582245817574695
10-11	23.358764306328347	27.310368450018913	29.53865744974878	19.79220979390396
12-13	24.475993001395707	24.80628780710322	29.600182925408287	21.117536266092788
14-15	21.631581351852915	26.219097015763932	27.417831812454384	24.731489819928765
16-17	21.094930165087412	29.480288102903383	27.94116968431292	21.483612047696276
18-19	20.987460334051143	28.46512476791108	29.960380774650343	20.587034123387433
20-21	21.843438997499565	27.591604943142716	29.856205431393224	20.7087506279645
22-23	21.570002892431212	27.555570272175817	29.700203600851875	21.1742232345411
24-25	21.34416446002085	27.66965105910057	29.82011319849289	21.166071282385683
26-27	21.34467872192095	27.871296637296382	29.66511436674893	21.118910274033738
28-29	21.23366948705189	27.81373337022586	29.760200881888387	21.192396260833863
30-31	21.530251803452792	27.7324344674527	29.62934549310975	21.10796823598476
32-33	21.468847598908088	27.732294913212396	29.594117008133907	21.204740479745602
34-35	21.455833497328317	27.762602079264997	29.642700895015313	21.138863528391376
36-37	21.349709188292906	27.828447832047594	29.607802665275955	21.21404031438355
38-39	21.238582854801173	27.79220751881704	29.707271759135324	21.26193786724646
40-41	21.496218829631726	27.670030679111544	29.60769714422945	21.226053347027282
42-43	21.47588999940239	27.7115648806297	29.47239702746267	21.34014809250524
44-45	21.532703450150155	27.865030845666006	29.313003013529826	21.289262690654013
46-47	21.580058064753576	27.83180762600707	29.050485309559797	21.537648999679547
48-49	21.56291366416262	27.873967196271522	29.05454807396849	21.508571065597373
50-51	21.479446352023693	28.034328645109973	29.00310682591039	21.483118176955944
52-53	21.618673332538922	27.959838380655004	28.931152243749338	21.490336043056733
54-55	21.619741565961924	27.872294129143153	28.91235186669888	21.595612438196042
56-57	21.669899050484673	28.060359036258053	28.771411186485206	21.498330726772068
58-59	21.55535374495667	28.186117062527437	28.899297169669776	21.35923202284612
60-61	21.670815490376643	28.20851962424809	28.786061761177233	21.334603124198033
62-63	21.599355818969197	28.2872549149786	28.71584901580292	21.397540250249282
64-65	21.565318190215994	28.25881101538788	28.7219576007824	21.453913193613722
66-67	21.620763204454295	28.205950330426038	28.615263640115746	21.55802282500392
68-69	21.60973202684697	28.171325558829132	28.713796882887955	21.50514553143595
70-71	21.619844397674147	27.94888050616226	28.7667754581318	21.664499638031792
72-73	21.693834421139428	27.916580637270695	28.747479941451793	21.642105000138084
74-75	21.607887903468455	28.0164020631956	28.694501868050292	21.68120816528565
76-77	21.716085661132766	27.984052857626796	28.58101291943035	21.71884856181009
78-79	21.72109236962269	27.98543934021304	28.533717158968418	21.759751131195852
80-81	21.667765484485113	28.053198508861517	28.53212310176002	21.746912904893353
82-83	21.746234941765454	27.9719096283542	28.58312417386937	21.698731256010976
84-85	21.88146270295813	27.979665772065648	28.51702532401979	21.621846200956433
86-87	21.696972791645344	28.007345456523357	28.561362826534115	21.73431892529719
88-89	21.81359633139692	27.983394425891067	28.48042573746479	21.722583505247226
90-91	21.828995495797372	28.0294025318063	28.485113557403015	21.656488414993316
92-93	21.81748920222854	28.020437500426905	28.475607395989627	21.68646590135493
94-95	21.739447245073425	28.11906637149686	28.444832873629117	21.696653509800598
96-97	21.83360678057965	28.20740666784361	28.507970624590573	21.451015926986162
98-99	22.875251564736384	30.11706251568421	28.762056507764434	18.245629411814974
100	21.763811861919905	31.068136942532988	22.802778956307396	24.36527223923971
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1388.0
1	1313.0
2	1922.0
3	2892.0
4	3742.0
5	5114.0
6	6371.5
7	8219.0
8	10855.0
9	12492.5
10	12478.5
11	12520.0
12	14049.5
13	16956.0
14	22005.5
15	27830.0
16	32145.0
17	34529.0
18	34447.5
19	32228.5
20	30007.0
21	29218.0
22	31345.0
23	37470.0
24	47584.0
25	61258.5
26	78981.5
27	99841.5
28	121153.5
29	143725.5
30	166019.5
31	187037.5
32	209623.5
33	230853.0
34	250660.0
35	268619.5
36	286001.5
37	304211.0
38	317367.5
39	325197.5
40	334284.5
41	342724.0
42	348745.0
43	359465.5
44	364591.0
45	363930.5
46	361749.0
47	353768.5
48	344332.5
49	331711.0
50	318055.0
51	304839.5
52	288633.0
53	270624.5
54	252301.5
55	231607.0
56	209718.5
57	186219.5
58	161915.0
59	139204.0
60	115892.5
61	93228.5
62	72773.5
63	55394.5
64	41168.0
65	29686.0
66	21378.0
67	16243.0
68	12165.5
69	8597.0
70	6257.0
71	4569.5
72	3108.0
73	1949.5
74	1200.5
75	706.5
76	429.5
77	254.0
78	160.5
79	107.0
80	73.0
81	47.5
82	34.0
83	25.5
84	17.0
85	9.5
86	5.5
87	5.5
88	5.0
89	4.0
90	3.5
91	3.0
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007741104248331856
3	0.0
4	0.004072443814696192
5	0.0027341903231812965
6	0.0
7	0.0
8	0.0
9	1.9612335651511407E-4
10-11	4.6723505522718354E-4
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	9.287536892721277E-4
24-25	0.001534566189964641
26-27	0.0
28-29	0.002181644405813563
30-31	0.003292708626018545
32-33	7.233381046643619E-4
34-35	1.7399995267201286E-5
36-37	0.0012161984128203373
38-39	0.0022980242780125215
40-41	0.0017636430280692605
42-43	0.0
44-45	0.0
46-47	0.0014985310798942444
48-49	5.856890208668318E-4
50-51	0.0018419577177694027
52-53	0.002605827486635347
54-55	0.0020066167880823703
56-57	0.00203852894051207
58-59	0.002183449564592084
60-61	9.295355525366586E-4
62-63	8.978492842370797E-4
64-65	0.0034950114600088883
66-67	0.002629613488506184
68-69	8.405188535814217E-5
70-71	0.0030899167736783938
72-73	0.010243572158470559
74-75	0.012571771035328399
76-77	0.005045042351662408
78-79	0.004472325451862915
80-81	3.9315967244647537E-4
82-83	6.832108916933785E-5
84-85	2.0653107717866707E-5
86-87	0.0020327316979393997
88-89	0.004427341554852978
90-91	5.5023629121552E-4
92-93	9.889795659034281E-4
94-95	0.002361819557272976
96-97	0.0023649850070865865
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	77.0
20-21	287.0
22-23	561.0
24-25	796.0
26-27	2054.0
28-29	5365.0
30-31	14190.0
32-33	18545.0
34-35	24822.0
36-37	39014.0
38-39	45982.0
40-41	45558.0
42-43	51724.0
44-45	61740.0
46-47	61809.0
48-49	57320.0
50-51	51832.0
52-53	50156.0
54-55	53098.0
56-57	54679.0
58-59	53842.0
60-61	53693.0
62-63	53134.0
64-65	56356.0
66-67	61903.0
68-69	63261.0
70-71	66736.0
72-73	57201.0
74-75	55650.0
76-77	57337.0
78-79	58427.0
80-81	58490.0
82-83	55779.0
84-85	55303.0
86-87	57703.0
88-89	60159.0
90-91	61015.0
92-93	60803.0
94-95	73844.0
96-97	260516.0
98-99	599227.0
100-101	6048026.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.26437511345006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.19042877614817	73.43120943033202
2	8.06195251754015	13.425468771345722
3	2.1350291974705757	5.3331561592907475
4	0.765088854224027	2.5481858141291633
5	0.35040170036871404	1.4587989309945668
6	0.1796547603210648	0.8975304812574062
7	0.10086999463265059	0.58792139495493
8	0.058547623502368314	0.3899945028241792
9	0.04054089247802381	0.3038050870851798
>10	0.11514715046373929	1.3745847622830993
>50	0.0017606797731683293	0.09984243333939391
>100	4.870245573706795E-4	0.07589748097340138
>500	6.81213960982634E-5	0.046586726982305864
>1k	2.2707123948640303E-5	0.027018024207900764
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1536668030300828E-5	0.0
2	0.0	1.1536668030300828E-5	0.0	2.3073336060601657E-5	0.0
3	0.0	1.1536668030300828E-5	0.0	4.614667212120331E-5	0.0
4	0.0	3.4610004090902485E-5	0.0	4.614667212120331E-5	0.0
5	0.0	4.614667212120331E-5	0.0	4.614667212120331E-5	0.0
6	0.0	4.614667212120331E-5	0.0	4.614667212120331E-5	1.1536668030300828E-5
7	0.0	6.922000818180497E-5	0.0	6.922000818180497E-5	1.1536668030300828E-5
8	0.0	9.229334424240663E-5	0.0	6.922000818180497E-5	1.1536668030300828E-5
9	0.0	1.730500204545124E-4	0.0	8.07566762121058E-5	1.1536668030300828E-5
10-11	0.0	1.730500204545124E-4	0.0	1.2113501431815869E-4	1.1536668030300828E-5
12-13	0.0	1.730500204545124E-4	0.0	1.3267168234845953E-4	1.1536668030300828E-5
14-15	0.0	2.1919669257571572E-4	0.0	1.4420835037876035E-4	1.7305002045451242E-5
16-17	0.0	2.2496502659086616E-4	0.0	1.7305002045451244E-4	2.3073336060601657E-5
18-19	0.0	2.3650169462116697E-4	0.0	2.0189169053026447E-4	2.3073336060601657E-5
20-21	0.0	2.4227002863631738E-4	0.0	2.480383626514678E-4	2.884167007575207E-5
22-23	0.0	3.114900368181223E-4	0.0	3.0572170280297197E-4	3.4610004090902485E-5
24-25	0.0	3.5186837492417523E-4	0.0	3.749417109847769E-4	3.4610004090902485E-5
26-27	0.0	3.691733769696265E-4	0.0	4.5569838719688267E-4	3.4610004090902485E-5
28-29	0.0	3.807100449999273E-4	0.0	6.864317478028992E-4	3.4610004090902485E-5
30-31	0.0	4.6146672121203313E-4	0.0	0.0014997668439391074	3.4610004090902485E-5
32-33	0.0	4.6146672121203313E-4	0.0	0.0031783520423478784	3.4610004090902485E-5
34-35	0.0	4.960767253029356E-4	0.0	0.005289562291892929	3.4610004090902485E-5
36-37	0.0	5.306867293938381E-4	0.0	0.00835831598795295	3.4610004090902485E-5
38-39	0.0	5.595283994695901E-4	0.0	0.013059508210300538	4.614667212120331E-5
40-41	0.0	5.768334015150414E-4	0.0	0.018187557149769256	4.614667212120331E-5
42-43	0.0	5.99906737575643E-4	0.0	0.023038726056510753	4.614667212120331E-5
44-45	0.0	6.979684158332001E-4	0.0	0.028460960030752144	4.614667212120331E-5
46-47	0.0	7.498834219695538E-4	0.0	0.034402344066357074	5.191500613635373E-5
48-49	0.0	7.498834219695538E-4	0.0	0.040684059808855866	8.07566762121058E-5
50-51	0.0	8.652501022725621E-4	0.0	0.04642355215393053	8.07566762121058E-5
52-53	0.0	8.652501022725621E-4	0.0	0.05248607120385362	8.652501022725622E-5
54-55	0.0	8.652501022725621E-4	0.0	0.05790253684407985	9.229334424240663E-5
56-57	0.0	8.767867703028629E-4	0.0	0.06230377569763962	1.0383001227270745E-4
58-59	0.0	8.940917723483141E-4	0.0	0.06682038123150238	1.0383001227270745E-4
60-61	0.0	9.171651084089159E-4	0.0	0.07089859338021373	1.0383001227270745E-4
62-63	0.0	9.402384444695175E-4	0.0	0.07494219552483418	1.1536668030300828E-4
64-65	0.0	0.0010383001227270744	0.0	0.07914154268786368	1.1536668030300828E-4
66-67	0.0	0.0010498367907573753	0.0	0.08286788646165084	1.1536668030300828E-4
68-69	0.0	0.0010613734587876763	0.0	0.08728066198324091	1.1536668030300828E-4
70-71	0.0	0.0010786784608331273	0.0	0.09084549240460388	1.4997668439391075E-4
72-73	0.0	0.0010844467948482779	0.0	0.09349892605157306	1.615133524242116E-4
74-75	0.0	0.0011248251329543306	0.0	0.09598507801210289	1.615133524242116E-4
76-77	0.0	0.0012171184771967373	0.0	0.09815973993581459	1.67281686439362E-4
78-79	0.0	0.0012574968153027902	0.0	0.0995787501035416	1.730500204545124E-4
80-81	0.0	0.0012632651493179408	0.0	0.10002868015672334	1.730500204545124E-4
82-83	0.0	0.0012921068193936926	0.0	0.10035170686157174	1.7881835446966285E-4
84-85	0.0	0.001367095161590648	0.0	0.10065742856437473	1.8458668848481325E-4
86-87	0.0	0.0013959368316664002	0.0	0.10074395357460197	1.8458668848481325E-4
88	0.0	0.0014882301759088067	0.0	0.10076125857664743	1.9612335651511407E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11465	0.0	47.591778	1
GTATCAA	20770	0.0	38.48701	1
ATCAACG	24200	0.0	32.366455	3
TCAACGC	24430	0.0	32.150684	4
CAACGCA	24860	0.0	31.523708	5
AACGCAG	25680	0.0	30.634796	6
TATCAAC	25765	0.0	30.554264	2
ACGCAGA	28830	0.0	27.227428	7
CGCAGAG	29395	0.0	26.674587	8
GCAGAGT	34850	0.0	22.399712	9
GAGTACT	20975	0.0	20.159063	12-13
GTACATG	23280	0.0	19.935455	1
TACATGG	22660	0.0	19.791832	2
GTGGTAT	5700	0.0	19.70835	1
AGAGTAC	31170	0.0	19.423056	10-11
CAGAGTA	34450	0.0	19.008888	10-11
ACATGGG	23210	0.0	18.742113	3
CTTATAC	5740	0.0	18.664244	1
TGGTATC	5625	0.0	17.889196	2
GTACTTT	24420	0.0	17.306437	14-15
>>END_MODULE