FastQCFastQC Report
Fri 27 May 2016
SRR2138836_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138836_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8799990
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103050.045.786891
GTATCAA184800.037.3486631
TCAACGC215450.031.1893844
ATCAACG215800.031.0583173
CAACGCA222650.030.1601315
AACGCAG231500.029.1379746
TATCAAC234000.028.940392
ACGCAGA265900.025.270587
CGCAGAG268150.025.0423838
GTGGTAT52850.021.8070981
GCAGAGT323700.020.7314879
TGGTATC54350.019.9297432
GAGTACT197700.018.79086312-13
AGAGTAC291450.018.48426810-11
TACATGG222000.017.9944742
GTACATG226600.017.9351161
CAGAGTA317200.017.84418710-11
ACATGGG224750.016.9059243
GTACTTT225000.016.44374814-15
AGTACTT219500.016.37197712-13
Produced by FastQC (version 0.11.5)