##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138836_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8799990
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.088098281929867	32.0	28.0	33.0	18.0	34.0
2	31.4558529043783	33.0	32.0	33.0	27.0	34.0
3	31.797706020120476	33.0	31.0	33.0	29.0	34.0
4	32.23349810624785	33.0	33.0	33.0	31.0	34.0
5	32.64247448008464	33.0	33.0	34.0	31.0	34.0
6	36.54181834297539	38.0	37.0	38.0	34.0	38.0
7	37.2013310242398	38.0	38.0	38.0	36.0	38.0
8	37.34019459113022	38.0	38.0	38.0	37.0	38.0
9	37.388926010143194	38.0	38.0	38.0	37.0	38.0
10-11	37.43692288286691	38.0	38.0	38.0	37.0	38.0
12-13	37.45249699147385	38.0	38.0	38.0	37.0	38.0
14-15	37.47094729653102	38.0	38.0	38.0	37.0	38.0
16-17	37.42196133177424	38.0	38.0	38.0	37.0	38.0
18-19	37.460540750614484	38.0	38.0	38.0	37.0	38.0
20-21	37.493123723823714	38.0	38.0	38.0	37.0	38.0
22-23	37.499470653403606	38.0	38.0	38.0	37.0	38.0
24-25	37.498619347759856	38.0	38.0	38.0	37.0	38.0
26-27	37.47163378514377	38.0	38.0	38.0	37.0	38.0
28-29	37.4474320337018	38.0	38.0	38.0	37.0	38.0
30-31	37.35164904110443	38.0	38.0	38.0	37.0	38.0
32-33	37.414701883181756	38.0	38.0	38.0	37.0	38.0
34-35	37.421220840093596	38.0	38.0	38.0	37.0	38.0
36-37	37.40170481648573	38.0	38.0	38.0	37.0	38.0
38-39	37.383893520446534	38.0	38.0	38.0	37.0	38.0
40-41	37.36700278182183	38.0	38.0	38.0	37.0	38.0
42-43	37.34933514375048	38.0	38.0	38.0	37.0	38.0
44-45	37.315833112818524	38.0	38.0	38.0	37.0	38.0
46-47	37.29011313690237	38.0	38.0	38.0	37.0	38.0
48-49	37.261315595819724	38.0	38.0	38.0	37.0	38.0
50-51	37.22001800284217	38.0	38.0	38.0	37.0	38.0
52-53	37.19997861398315	38.0	38.0	38.0	37.0	38.0
54-55	37.18949595690787	38.0	38.0	38.0	37.0	38.0
56-57	37.17923146879741	38.0	38.0	38.0	37.0	38.0
58-59	37.168679874474975	38.0	38.0	38.0	37.0	38.0
60-61	37.171406241020534	38.0	38.0	38.0	37.0	38.0
62-63	37.177084582600784	38.0	38.0	38.0	36.5	38.0
64-65	37.18282335864806	38.0	38.0	38.0	37.0	38.0
66-67	37.16581463551497	38.0	38.0	38.0	36.0	38.0
68-69	37.16729280436699	38.0	38.0	38.0	36.0	38.0
70-71	37.149688328569894	38.0	38.0	38.0	36.0	38.0
72-73	37.14182078535628	38.0	38.0	38.0	36.0	38.0
74-75	37.13161784314357	38.0	38.0	38.0	36.0	38.0
76-77	37.13478026497767	38.0	38.0	38.0	36.0	38.0
78-79	37.11708165917038	38.0	38.0	38.0	36.0	38.0
80-81	37.11325356226893	38.0	38.0	38.0	36.0	38.0
82-83	37.06388739603127	38.0	38.0	38.0	36.0	38.0
84-85	37.05280316808552	38.0	38.0	38.0	36.0	38.0
86-87	37.04586203295108	38.0	38.0	38.0	36.0	38.0
88-89	37.051290796718796	38.0	38.0	38.0	36.0	38.0
90-91	37.03983490869845	38.0	38.0	38.0	36.0	38.0
92-93	37.02216149415757	38.0	38.0	38.0	36.0	38.0
94-95	36.99591632786204	38.0	38.0	38.0	36.0	38.0
96-97	36.96778170877899	38.0	38.0	38.0	36.0	38.0
98-99	36.97145989157577	38.0	38.0	38.0	35.5	38.0
100	35.44749278806333	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	11.0
19	24.0
20	97.0
21	221.0
22	584.0
23	1515.0
24	3422.0
25	6906.0
26	11766.0
27	19163.0
28	30114.0
29	44228.0
30	61082.0
31	80906.0
32	105578.0
33	142008.0
34	209521.0
35	381569.0
36	1152613.0
37	6548661.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.34709130351285	21.15035357994725	14.798300907160122	23.704254209379783
2	13.756976399285852	21.732976790225596	41.82118825226069	22.68885855822786
3	17.829236169586558	26.47238235497995	30.776819064567118	24.921562410866375
4	12.2514320364794	18.73367132947396	39.947732344694245	29.067164289352394
5	13.103919605116682	38.06279350218938	35.80617616563354	13.027110727060407
6	28.933771515649447	38.820521386956116	19.337771974740882	12.90793512265355
7	25.80427932304469	33.10594671130308	23.64316323086731	17.446610734784926
8	23.811208876373723	36.93374651562104	21.694547380167478	17.56049722783776
9	25.00387217625169	17.452565982962877	21.911233470239246	35.63232837054619
10-11	23.516009625502672	27.32018205545573	29.436652865082507	19.72715545395909
12-13	24.51808746579115	24.73679228462672	29.511457343867136	21.233662905714993
14-15	21.644723459913024	26.258490066465985	27.277673042810274	24.819113430810717
16-17	21.225268437805042	29.36034018220475	27.90221352524264	21.512177854747563
18-19	21.15970018147748	28.536333564015415	29.677562133593334	20.626404120913776
20-21	21.965264844396582	27.665702678768163	29.590559123208514	20.77847335362674
22-23	21.6979407606231	27.552732755041443	29.442792747412632	21.306533736922816
24-25	21.476357517045056	27.671959394744345	29.495135191837168	21.356547896373428
26-27	21.506130528766523	27.801806308240817	29.3860242095519	21.306038953440762
28-29	21.386712027027478	27.910310960531447	29.422196026545635	21.280780985895436
30-31	21.71465877875165	27.69894250334938	29.334069686419873	21.252329031479096
32-33	21.534946583929106	27.767344982356146	29.31235569087884	21.38535274283591
34-35	21.576784016528322	27.876383835521263	29.312401725966286	21.23443042198413
36-37	21.566296463759727	27.772132735464677	29.314618728006646	21.346952072768946
38-39	21.425727488282075	27.866160193653027	29.402591011131708	21.30552130693319
40-41	21.72984547448576	27.75438179652933	29.214304548259882	21.30146818072502
42-43	21.62220315076839	27.815585905405616	29.10782778018729	21.454383163638706
44-45	21.680167639392252	27.905580102186057	28.96554159994799	21.448710658473697
46-47	21.67719777247633	27.87832851303844	28.81260772061436	21.631865993870868
48-49	21.668938891737856	27.94361546447199	28.804797700496316	21.58264794329384
50-51	21.663175695451365	28.08248639996622	28.67449810141705	21.57983980316536
52-53	21.797056789393707	28.01744945772875	28.579801249406383	21.60569250347116
54-55	21.82889097174905	27.91720424266706	28.61233755604674	21.641567229537145
56-57	21.803158405339616	28.03129403771819	28.55934283671065	21.606204720231542
58-59	21.72810390921475	28.11251097093617	28.60154176004748	21.557843359801605
60-61	21.823799589821217	28.0449209863228	28.594827116027925	21.536452307828053
62-63	21.76686548705539	28.090513359932956	28.517933255874304	21.624687897137342
64-65	21.74205049740105	28.03830970080195	28.54102025248323	21.678619549313773
66-67	21.813798760487003	28.06368032730211	28.4029997200699	21.71952119214099
68-69	21.786209949013973	28.04592510935842	28.480350611187294	21.68751433044031
70-71	21.767784388220882	27.86802470600451	28.5593929570082	21.804797948766407
72-73	21.807306931976473	27.900465225091054	28.52092753851104	21.77130030442144
74-75	21.77104425211879	27.979586955997434	28.41617270588718	21.833196085996594
76-77	21.847914000373677	27.93946638730535	28.335036533798153	21.87758307852282
78-79	21.822202126581164	27.969071772794685	28.3375646371124	21.87116146351175
80-81	21.790487655780957	28.016137626795494	28.33061414776923	21.86276056965432
82-83	21.87685392821772	27.95804546738608	28.39855213709156	21.76654846730464
84-85	22.04779467313982	27.91187505244432	28.323942882376357	21.716387392039508
86-87	21.821434700814304	27.97514019060549	28.33518481253362	21.86824029604659
88-89	21.931831516752002	27.955320074411254	28.28006419415151	21.832784214685237
90-91	21.95175947631505	27.961679990910376	28.31776456622494	21.768795966549632
92-93	21.9652223658337	27.968204784498173	28.293222042953975	21.773350806714152
94-95	21.905192588619446	28.079718102040914	28.232200664185058	21.78288864515458
96-97	21.979959285555005	28.177028708719078	28.2518397030579	21.591172302668017
98-99	23.026612270258397	30.09369102842169	28.526412213551115	18.353284487768793
100	21.91564525472701	31.008615768859922	22.52948897110357	24.5462500053095
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1144.0
1	1157.0
2	1764.0
3	2663.0
4	3440.0
5	4528.5
6	5549.5
7	7276.0
8	9647.0
9	11091.0
10	11320.0
11	11594.5
12	12921.5
13	15585.0
14	20621.5
15	26231.0
16	29934.5
17	31594.5
18	31273.5
19	29268.5
20	27107.0
21	26481.5
22	28550.5
23	34287.0
24	43673.0
25	57682.0
26	75531.5
27	95208.0
28	115625.0
29	137718.5
30	160410.5
31	183344.0
32	206454.5
33	228226.0
34	250198.0
35	270329.5
36	291257.5
37	311314.0
38	325707.0
39	335572.0
40	345715.5
41	356397.5
42	362882.5
43	367727.0
44	370363.0
45	371124.5
46	370630.5
47	368035.5
48	363036.5
49	352308.0
50	337759.0
51	320540.0
52	300833.0
53	281764.5
54	261748.0
55	238757.5
56	214255.0
57	189958.5
58	166454.0
59	142977.5
60	118197.5
61	94486.5
62	73446.5
63	55528.0
64	41438.5
65	30371.5
66	22374.5
67	16868.0
68	12577.0
69	9039.0
70	6401.5
71	4504.0
72	3123.0
73	2081.5
74	1342.0
75	845.0
76	528.0
77	317.0
78	189.0
79	113.0
80	69.0
81	47.5
82	38.5
83	33.5
84	29.0
85	20.5
86	14.0
87	9.5
88	5.0
89	2.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007727281508274441
3	0.0
4	0.0032272763946322664
5	0.0025681847365735646
6	0.0
7	0.0
8	0.0
9	3.295458290293512E-4
10-11	3.863640754137221E-4
12-13	1.1363649276874177E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.068542610399681E-4
24-25	0.0015001548739435203
26-27	0.0
28-29	0.0019612263838498633
30-31	0.00300988351295624
32-33	6.441089453850335E-4
34-35	2.285442122206704E-5
36-37	0.001129505357496186
38-39	0.0021667646993230763
40-41	0.001541464586068081
42-43	0.0
44-45	0.0
46-47	0.0013656100490455007
48-49	5.658195553789934E-4
50-51	0.0015953216054405022
52-53	0.002420593077900601
54-55	0.0017073328853761715
56-57	0.0018049173167377203
58-59	0.0018423192815718841
60-61	7.81806833773534E-4
62-63	7.434312074053745E-4
64-65	0.0031327603443369128
66-67	0.0023131565245209803
68-69	7.02550340765656E-5
70-71	0.0028386985809339357
72-73	0.009659223377681863
74-75	0.011456481512584943
76-77	0.0047283886461510355
78-79	0.004135648198795763
80-81	3.145491496766067E-4
82-83	3.373460875342929E-5
84-85	7.479896927020346E-5
86-87	0.0016586273501618667
88-89	0.004464254863342663
90-91	5.646129463239201E-4
92-93	9.773485069384362E-4
94-95	0.002228044940943682
96-97	0.002618316244264161
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	48.0
20-21	233.0
22-23	483.0
24-25	718.0
26-27	1954.0
28-29	5267.0
30-31	15197.0
32-33	19227.0
34-35	26666.0
36-37	41483.0
38-39	48765.0
40-41	47944.0
42-43	54307.0
44-45	63605.0
46-47	64010.0
48-49	59351.0
50-51	54592.0
52-53	53061.0
54-55	56606.0
56-57	57494.0
58-59	56695.0
60-61	55417.0
62-63	54610.0
64-65	57458.0
66-67	60791.0
68-69	60974.0
70-71	60979.0
72-73	57267.0
74-75	57557.0
76-77	59933.0
78-79	60844.0
80-81	60894.0
82-83	58268.0
84-85	57392.0
86-87	59451.0
88-89	61703.0
90-91	62115.0
92-93	63015.0
94-95	75483.0
96-97	264996.0
98-99	602035.0
100-101	6121102.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.52845640027638
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.24344054810787	73.70838376433011
2	8.136958507642824	13.59335167873003
3	2.030986042235034	5.0893538723519685
4	0.7408699982376434	2.475349093842634
5	0.35519993287959223	1.4834651053457057
6	0.1808935948587669	0.9065857650749863
7	0.10174380631235856	0.5948952162692006
8	0.05704110434423537	0.3811644317792864
9	0.04107434432186202	0.30877889209727327
>10	0.11049521551290717	1.3335119858425106
>50	8.972422639366108E-4	0.047064770706603386
>100	3.5577160030733927E-4	0.05047057671063031
>500	4.3891682584145006E-5	0.027624846919004974
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.2727298553748358E-5	0.0
2	0.0	2.2727298553748358E-5	0.0	2.2727298553748358E-5	0.0
3	0.0	2.2727298553748358E-5	0.0	2.2727298553748358E-5	0.0
4	0.0	7.954554493811924E-5	0.0	5.681824638437089E-5	0.0
5	0.0	9.090919421499343E-5	0.0	5.681824638437089E-5	0.0
6	1.1363649276874179E-5	1.022728434918676E-4	0.0	7.954554493811924E-5	0.0
7	1.1363649276874179E-5	1.022728434918676E-4	0.0	7.954554493811924E-5	0.0
8	2.2727298553748358E-5	1.1363649276874178E-4	0.0	7.954554493811924E-5	1.1363649276874179E-5
9	2.2727298553748358E-5	1.7045473915311266E-4	0.0	2.1590933626060939E-4	1.1363649276874179E-5
10-11	2.2727298553748358E-5	1.8181838842998686E-4	0.0	2.443184594527948E-4	1.1363649276874179E-5
12-13	3.4090947830622535E-5	1.8181838842998686E-4	0.0	2.8409123192185443E-4	1.1363649276874179E-5
14-15	3.4090947830622535E-5	2.2159116089904646E-4	0.0	2.9545488119872863E-4	2.2727298553748358E-5
16-17	3.4090947830622535E-5	2.2727298553748356E-4	0.0	3.295458290293512E-4	3.4090947830622535E-5
18-19	3.4090947830622535E-5	2.3863663481435773E-4	0.0	3.6931860149841075E-4	3.4090947830622535E-5
20-21	3.4090947830622535E-5	2.613639333681061E-4	0.0	4.204550232443446E-4	3.4090947830622535E-5
22-23	3.4090947830622535E-5	2.9545488119872863E-4	0.0	4.43182321798093E-4	3.977277246905962E-5
24-25	4.5454597107496716E-5	3.125003551140399E-4	0.0	4.659096203518413E-4	4.5454597107496716E-5
26-27	5.11364217459338E-5	3.18182179752477E-4	0.0	5.454551652899606E-4	4.5454597107496716E-5
28-29	5.681824638437089E-5	3.18182179752477E-4	0.0	8.579555204040006E-4	4.5454597107496716E-5
30-31	6.818189566124507E-5	3.693186014984108E-4	0.0	0.0017500019886386234	4.5454597107496716E-5
32-33	7.954554493811924E-5	3.863640754137221E-4	0.0	0.0036306859439613	5.681824638437089E-5
34-35	1.022728434918676E-4	4.4886414643653E-4	0.0	0.005926143097889884	5.681824638437089E-5
36-37	1.0795466813030469E-4	4.886369189055896E-4	0.0	0.009659101885343052	5.681824638437089E-5
38-39	1.4772744059936432E-4	4.886369189055896E-4	0.0	0.014852289604874551	7.386372029968216E-5
40-41	1.4772744059936432E-4	5.568188145668347E-4	0.0	0.020403432276627588	9.090919421499343E-5
42-43	1.4772744059936432E-4	5.79546113120583E-4	0.0	0.025875029403442502	9.090919421499343E-5
44-45	1.4772744059936432E-4	6.647734826971394E-4	0.0	0.031312535582426804	9.090919421499343E-5
46-47	1.590910898762385E-4	7.272735537199473E-4	0.0	0.03750572443832323	9.090919421499343E-5
48-49	1.7045473915311266E-4	7.443190276352587E-4	0.0	0.04352845855506654	9.090919421499343E-5
50-51	1.9318203770686104E-4	7.840918001043183E-4	0.0	0.04939210158193361	9.090919421499343E-5
52-53	1.9318203770686104E-4	7.840918001043183E-4	0.0	0.055630745034937534	9.659101885343052E-5
54-55	1.9318203770686104E-4	7.897736247427554E-4	0.0	0.06168188827487304	1.022728434918676E-4
56-57	1.9318203770686104E-4	8.068190986580667E-4	0.0	0.06618189338851521	1.022728434918676E-4
58-59	1.9318203770686104E-4	8.125009232965038E-4	0.0	0.0704546255166199	1.022728434918676E-4
60-61	2.045456869837352E-4	8.29546397211815E-4	0.0	0.07451144830846398	1.022728434918676E-4
62-63	2.045456869837352E-4	8.29546397211815E-4	0.0	0.07871031671626899	1.2500014204561597E-4
64-65	2.2727298553748356E-4	8.636373450424375E-4	0.0	0.08259668476895997	1.2500014204561597E-4
66-67	2.2727298553748356E-4	8.750009943193117E-4	0.0	0.08651146194484312	1.3068196668405307E-4
68-69	2.2727298553748356E-4	8.86364643596186E-4	0.0	0.09059101203524095	1.3636379132249014E-4
70-71	2.2727298553748356E-4	8.977282928730601E-4	0.0	0.09426147075167131	1.3636379132249014E-4
72-73	2.2727298553748356E-4	8.977282928730601E-4	0.0	0.09714783766799735	1.4772744059936432E-4
74-75	2.2727298553748356E-4	9.318192407036826E-4	0.0	0.0994546584712028	1.4772744059936432E-4
76-77	2.2727298553748356E-4	9.602283638958681E-4	0.0	0.10186375211790014	1.4772744059936432E-4
78-79	2.2727298553748356E-4	9.772738378111792E-4	0.0	0.1032671628035941	1.4772744059936432E-4
80-81	2.2727298553748356E-4	9.772738378111792E-4	0.0	0.10377852702105345	1.4772744059936432E-4
82-83	2.2727298553748356E-4	0.0010113647856418019	0.0	0.10411375467472123	1.4772744059936432E-4
84-85	2.3863663481435773E-4	0.0010625012073877357	0.0	0.10430125488778964	1.4772744059936432E-4
86-87	2.500002840912319E-4	0.0011079558044952325	0.0	0.1043864822573662	1.4772744059936432E-4
88	2.613639333681061E-4	0.0011704558755180404	0.0	0.10439784590664307	1.7045473915311266E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10305	0.0	45.78689	1
GTATCAA	18480	0.0	37.348663	1
TCAACGC	21545	0.0	31.189384	4
ATCAACG	21580	0.0	31.058317	3
CAACGCA	22265	0.0	30.160131	5
AACGCAG	23150	0.0	29.137974	6
TATCAAC	23400	0.0	28.94039	2
ACGCAGA	26590	0.0	25.27058	7
CGCAGAG	26815	0.0	25.042383	8
GTGGTAT	5285	0.0	21.807098	1
GCAGAGT	32370	0.0	20.731487	9
TGGTATC	5435	0.0	19.929743	2
GAGTACT	19770	0.0	18.790863	12-13
AGAGTAC	29145	0.0	18.484268	10-11
TACATGG	22200	0.0	17.994474	2
GTACATG	22660	0.0	17.935116	1
CAGAGTA	31720	0.0	17.844187	10-11
ACATGGG	22475	0.0	16.905924	3
GTACTTT	22500	0.0	16.443748	14-15
AGTACTT	21950	0.0	16.371977	12-13
>>END_MODULE