FastQCFastQC Report
Fri 27 May 2016
SRR2138835_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138835_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7249390
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55400.033.48321
GTATCAA87650.031.029741
TCAACGC89500.028.2121544
ATCAACG92250.027.4195233
CAACGCA93250.026.9826535
AACGCAG98950.025.4693766
TATCAAC106900.024.026372
ACGCAGA119850.021.0273287
CGCAGAG121250.020.746748
GTACATG195400.019.3625661
TACATGG193050.018.8367332
ACATGGG194800.018.0678253
CATGGGG143150.016.0379774
GCAGAGT159750.015.8006389
AGAGTAC128700.015.23015610-11
GTATAAG30450.014.910131
GTGTAGC39500.014.4496131
CAGAGTA153650.014.09323710-11
GTATAGC30800.013.8983711
GTATAGG25650.013.1488161
Produced by FastQC (version 0.11.5)