##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138835_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7249390
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.409107938736913	31.0	31.0	34.0	29.0	34.0
2	31.669414392107473	33.0	31.0	34.0	30.0	34.0
3	31.764269131609694	33.0	31.0	34.0	30.0	34.0
4	35.33122510997477	37.0	35.0	37.0	33.0	37.0
5	35.32998086735574	37.0	35.0	37.0	33.0	37.0
6	35.462590921443045	37.0	35.0	37.0	33.0	37.0
7	35.38235975716577	37.0	35.0	37.0	33.0	37.0
8	35.41166829209078	37.0	35.0	37.0	33.0	37.0
9	37.00601002291227	39.0	37.0	39.0	33.0	39.0
10-11	37.0114039250199	39.0	37.0	39.0	33.0	39.0
12-13	36.9558040055784	39.0	37.0	39.0	33.0	39.0
14-15	38.31685748731962	40.0	38.0	41.0	33.0	41.0
16-17	38.31071469461568	40.0	38.0	41.0	33.0	41.0
18-19	38.32957607467663	40.0	38.0	41.0	33.0	41.0
20-21	38.38847279037307	40.0	38.0	41.0	33.0	41.0
22-23	38.36027782436604	40.0	38.0	41.0	33.0	41.0
24-25	38.39316596800401	40.0	38.0	41.0	33.0	41.0
26-27	38.38439348301203	40.0	38.0	41.0	33.0	41.0
28-29	38.319508506443654	40.0	38.0	41.0	33.0	41.0
30-31	38.25131592722272	40.0	38.0	41.0	33.0	41.0
32-33	38.21803516759789	40.0	38.0	41.0	33.0	41.0
34-35	38.144217621183344	40.0	38.0	41.0	33.0	41.0
36-37	38.06003375405004	40.0	38.0	41.0	32.0	41.0
38-39	37.97487290528494	40.0	38.0	41.0	32.0	41.0
40-41	37.93511662446255	40.0	37.5	41.0	32.0	41.0
42-43	37.77719406426603	40.0	37.0	41.0	32.0	41.0
44-45	37.59046235208458	40.0	37.0	41.0	31.0	41.0
46-47	37.44815836757161	40.0	36.5	41.0	31.0	41.0
48-49	37.31692828753011	39.5	36.0	41.0	31.0	41.0
50-51	37.18604946146694	39.0	36.0	41.0	31.0	41.0
52-53	37.04055886963476	39.0	36.0	40.0	30.0	41.0
54-55	36.86938995145732	39.0	35.0	40.0	30.0	41.0
56-57	36.84875900956317	39.0	35.0	40.0	30.0	41.0
58-59	36.918671079847364	39.0	35.0	40.0	30.0	41.0
60-61	37.214437784402875	39.0	35.0	41.0	31.0	41.0
62-63	37.176025533402324	39.0	35.0	41.0	31.0	41.0
64-65	36.96311812599498	39.0	35.0	41.0	31.0	41.0
66-67	36.74559212204175	38.0	35.0	40.0	31.0	41.0
68-69	36.45427342664729	37.5	35.0	40.0	31.0	41.0
70-71	36.1958377661465	37.0	35.0	40.0	31.0	41.0
72-73	35.886211534997244	36.5	34.5	39.0	30.0	41.0
74-75	35.571257703059935	36.0	34.0	39.0	30.0	41.0
76-77	35.262487169081	36.0	34.0	38.5	30.0	40.0
78-79	34.949403155780864	35.0	34.0	37.0	30.0	39.5
80-81	34.64840244601409	35.0	34.0	37.0	30.0	39.0
82-83	34.3971726458238	35.0	34.0	36.5	30.0	39.0
84-85	34.16424658129255	35.0	34.0	36.0	30.0	37.5
86-87	33.967019820022074	35.0	34.0	36.0	30.0	37.0
88-89	33.821065802429274	35.0	34.0	35.5	30.0	37.0
90-91	33.698061920072035	35.0	34.0	35.0	30.0	36.0
92-93	33.62935534411098	35.0	34.0	35.0	30.0	36.0
94-95	33.604908183576335	35.0	34.0	35.0	30.0	36.0
96-97	33.60563389850515	35.0	34.0	35.0	30.0	36.0
98-99	33.64050944353428	35.0	34.0	35.0	30.0	36.0
100	32.93522575429125	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	32.0
17	207.0
18	1029.0
19	3530.0
20	9133.0
21	18168.0
22	29412.0
23	41074.0
24	53839.0
25	67567.0
26	77575.0
27	81670.0
28	90731.0
29	109118.0
30	127949.0
31	149945.0
32	188869.0
33	244148.0
34	329496.0
35	466095.0
36	737214.0
37	1335252.0
38	1933784.0
39	1108501.0
40	45050.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.85324420399509	20.519781664388315	14.73379139486219	23.893182736754405
2	13.894302607096773	20.883565717388596	42.276046238694825	22.946085436819804
3	17.950941076724504	26.01857249391713	30.29758623805933	25.732900191299034
4	12.230833311948176	18.19998239518721	39.63373159393368	29.935452698930938
5	13.30281070400936	38.045951148433815	35.76508658003581	12.886151567521006
6	29.581182566875242	38.079848131687264	19.419305127969885	12.919664173467607
7	26.37319186339043	32.673102862928666	23.40945303276903	17.544252240911877
8	23.919087286895017	37.20764537653857	21.550509266897347	17.32275806966906
9	24.833413478051796	18.05500493421098	21.679449818232882	35.432131769504345
10-11	23.588244718847665	27.406421934760427	29.30108518808173	19.704248158310183
12-13	24.249750471484912	24.780700801464	29.254867263107236	21.714681463943844
14-15	21.60102618494517	26.44830578854689	26.583365698190143	25.3673023283178
16-17	21.595123649940515	29.50956405578895	27.275372648593017	21.619939645677515
18-19	21.646391278381618	29.09830256717515	28.30667027984498	20.94863587459825
20-21	22.48989099986737	28.24800838999742	28.04089447485989	21.221206135275324
22-23	22.18767479149317	28.11288025997235	27.872947214177497	21.82649773435698
24-25	21.9607385137192	28.238208786686492	27.95194194499605	21.84911075459826
26-27	21.96251878615646	28.377871462328187	27.834348277477105	21.825261474038253
28-29	21.84484014569561	28.447859222313642	27.894038902253932	21.81326172973682
30-31	22.177794902372472	28.21428388148911	27.80809256379732	21.799828652341095
32-33	21.905611148540487	28.316488724889645	27.859306205938715	21.918593920631153
34-35	22.03550187467359	28.280824934327963	27.86997799610923	21.813695194889217
36-37	21.995806542321354	28.12696478950711	27.92602565979781	21.95120300837373
38-39	21.83130472200692	28.17065504097934	28.121751955318526	21.87628828169521
40-41	22.02173478243123	28.028892503445345	28.08714567134774	21.862227042775682
42-43	21.854800701725352	28.031751444149283	28.076809197786623	22.03663865633874
44-45	21.858489727318954	28.092952729018894	28.100760159144162	21.947797384517987
46-47	21.78548521303454	28.072491758338515	28.022111523100307	22.119911505526634
48-49	21.65094329834463	28.073223052347252	28.195818170538516	22.080015478769607
50-51	21.57721470808422	28.230258914287504	28.134882504479762	22.05764387314851
52-53	21.763565730257632	28.136208326446287	28.064624050537223	22.035601892758855
54-55	21.768501477153173	28.025421597693935	28.074493940554962	22.131582984597934
56-57	21.7696094509588	28.07017787903991	28.025986329612145	22.134226340389144
58-59	21.751570826256675	28.121270559668982	28.01340990969998	22.113748704374363
60-61	21.866624268322084	28.037894015778274	28.014327272376054	22.081154443523584
62-63	21.838486387815212	28.12747416685332	27.89775469919521	22.136284746136255
64-65	21.95860088748492	28.09383289802615	27.75986224087641	22.187703973612514
66-67	21.946695625038096	28.133633113657268	27.68238747984172	22.237283781462914
68-69	21.96720911257536	28.08148695670065	27.731563129237202	22.219740801486783
70-71	22.008912483466236	27.956294072438393	27.831268571063738	22.20352487303163
72-73	22.094052646421172	27.938876301131486	27.799658702938185	22.167412349509156
74-75	21.995164660025328	28.10074904639247	27.788535792195713	22.11555050138649
76-77	22.088933126414958	28.02097463280862	27.808167794508055	22.08192444626837
78-79	22.115314115875158	28.012510501321202	27.785244307323005	22.08693107548064
80-81	22.038941660749895	28.110622056565283	27.799854531567288	22.050581751117537
82-83	22.09695374610342	28.05913286210579	27.897427996529423	21.946485395261366
84-85	22.170689541502764	28.12563221521173	27.90053742115085	21.80314082213465
86-87	21.917402883226348	28.157007939869878	28.019582545863337	21.906006631040437
88-89	21.99630703492484	28.161127906692897	27.966251023292738	21.87631403508953
90-91	21.98518955508731	28.20207094643214	27.988449713725593	21.82428978475496
92-93	21.950383898465528	28.19364041971262	28.014273713258337	21.841701968563516
94-95	21.839378461454547	28.302802679238486	28.019678393777436	21.83814046552953
96-97	21.8757880746347	28.432770635825943	28.103098856082177	21.58834243345718
98-99	22.909277978623084	30.378067036137136	28.28058743993419	18.432067545305596
100	21.95210268102288	31.381904390290543	22.0992906671285	24.56670226155808
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1790.0
1	1343.5
2	971.5
3	1073.0
4	1099.5
5	1193.0
6	1419.5
7	1710.0
8	1942.0
9	2158.5
10	2443.5
11	2713.5
12	2992.0
13	3367.0
14	3861.0
15	4493.5
16	5360.5
17	6468.5
18	7993.0
19	10089.5
20	12864.0
21	16785.5
22	22142.0
23	28973.5
24	38032.5
25	49775.5
26	64701.5
27	81551.0
28	98768.5
29	117198.0
30	134995.5
31	151919.0
32	169339.0
33	185969.5
34	202523.0
35	217644.5
36	233873.0
37	249342.0
38	259140.5
39	267386.5
40	278090.0
41	287912.5
42	293794.0
43	298728.5
44	301909.5
45	304312.0
46	305504.5
47	303183.0
48	298528.5
49	291631.0
50	283990.0
51	273116.0
52	258164.5
53	242446.0
54	226490.0
55	208563.0
56	188904.5
57	169375.5
58	150015.5
59	130871.5
60	110419.0
61	90752.5
62	72661.5
63	56672.5
64	43677.0
65	33321.5
66	25679.0
67	20085.0
68	15819.5
69	12139.5
70	9392.0
71	7342.5
72	5713.0
73	4376.5
74	3314.5
75	2526.0
76	1908.0
77	1465.0
78	1112.5
79	834.0
80	633.0
81	486.0
82	393.5
83	292.0
84	207.0
85	154.0
86	122.5
87	102.5
88	70.0
89	43.0
90	28.5
91	23.5
92	19.5
93	12.5
94	7.5
95	7.5
96	8.5
97	5.5
98	2.0
99	5.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011435444913296153
3	0.02172596590885578
4	0.01892573030282548
5	0.010745731709840415
6	0.013863235389460353
7	0.01535301590892475
8	0.002013962554090758
9	5.517705627645912E-5
10-11	2.069139610367217E-5
12-13	0.00301404669910158
14-15	2.0001682900216432E-4
16-17	4.621078463153452E-4
18-19	2.069139610367217E-5
20-21	8.280685425455406E-5
22-23	6.905342463208854E-6
24-25	4.1463893828659165E-5
26-27	0.0024762838753731977
28-29	0.003649389887104358
30-31	0.0027946890367539054
32-33	0.004132921421147054
34-35	0.006012847450719704
36-37	0.0010603167459102294
38-39	0.001357817346410561
40-41	5.5498971582981E-4
42-43	4.933585481839331E-5
44-45	7.078354124111012E-5
46-47	0.004048184350188863
48-49	8.157199247104821E-4
50-51	3.525518711726537E-4
52-53	0.004144714028475415
54-55	2.4732516020669113E-4
56-57	1.9767552616649242E-4
58-59	7.300284674232171E-4
60-61	4.4577678871465536E-5
62-63	0.0
64-65	0.0
66-67	0.0017577428572863466
68-69	0.0
70-71	4.029065367634006E-4
72-73	0.0017131051133136897
74-75	1.2638917501993789E-4
76-77	3.991274116874723E-5
78-79	4.0374152112525026E-5
80-81	0.0031469759563680366
82-83	9.929163691365185E-5
84-85	3.0995465447176604E-4
86-87	0.013821242715530573
88-89	6.876342390966136E-4
90-91	8.885560772095994E-4
92-93	7.95103715979058E-5
94-95	2.0633222843920163E-4
96-97	5.4981921028000646E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2403.0
20-21	4869.0
22-23	5361.0
24-25	6317.0
26-27	7854.0
28-29	9337.0
30-31	11983.0
32-33	12763.0
34-35	16120.0
36-37	22074.0
38-39	26261.0
40-41	23976.0
42-43	27516.0
44-45	34881.0
46-47	40059.0
48-49	38089.0
50-51	37773.0
52-53	37799.0
54-55	42567.0
56-57	48144.0
58-59	50591.0
60-61	50933.0
62-63	51381.0
64-65	55023.0
66-67	57824.0
68-69	59131.0
70-71	61284.0
72-73	61660.0
74-75	64710.0
76-77	70059.0
78-79	74807.0
80-81	74691.0
82-83	73940.0
84-85	75096.0
86-87	76112.0
88-89	76505.0
90-91	79829.0
92-93	83223.0
94-95	100049.0
96-97	251923.0
98-99	475062.0
100-101	4769411.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.23274514772503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.59516764991221	79.02865171220216
2	6.856820903892889	11.96278620865764
3	1.5468031918961036	4.047956658970812
4	0.49965029590656723	1.7434346770321192
5	0.21227416637383426	0.9258629128367234
6	0.10658526795041502	0.5578635309372322
7	0.05839483462925674	0.35657592090102513
8	0.03770482043533733	0.2631275993501213
9	0.02301846532541672	0.180716752748144
>10	0.06264240639241782	0.8180398193656433
>50	6.001673366300948E-4	0.033686065459635255
>100	2.9269160295122734E-4	0.03914675668073683
>500	2.256917396608772E-5	0.0160061082363284
>1k	2.2569172007704626E-5	0.026145276621646594
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.138279220734434E-5	0.0	1.379426406911478E-5	0.0
3	0.0	4.138279220734434E-5	0.0	1.379426406911478E-5	0.0
4	0.0	1.1035411255291825E-4	0.0	2.758852813822956E-5	0.0
5	1.379426406911478E-5	1.3794264069114781E-4	0.0	4.138279220734434E-5	0.0
6	2.758852813822956E-5	1.3794264069114781E-4	0.0	4.138279220734434E-5	0.0
7	2.758852813822956E-5	1.3794264069114781E-4	0.0	4.138279220734434E-5	0.0
8	2.758852813822956E-5	1.5173690476026259E-4	0.0	4.138279220734434E-5	0.0
9	2.758852813822956E-5	2.3450248917495126E-4	0.0	6.897132034557391E-5	0.0
10-11	3.4485660172786954E-5	2.3450248917495126E-4	0.0	1.448397727257052E-4	0.0
12-13	6.207418831101651E-5	2.3450248917495126E-4	0.0	2.3450248917495126E-4	0.0
14-15	8.276558441468868E-5	3.310623376587547E-4	0.0	3.0347380952052517E-4	0.0
16-17	1.4483977272570521E-4	3.724451298660991E-4	0.0	4.0693079003888605E-4	0.0
18-19	1.5173690476026259E-4	4.06930790038886E-4	0.0	6.207418831101651E-4	0.0
20-21	1.5863403679481996E-4	4.552107142807878E-4	0.0	9.104214285615756E-4	0.0
22-23	1.9311969696760692E-4	5.103877705572469E-4	0.0	0.0014001178030151502	0.0
24-25	2.0691396103672172E-4	5.586676947991487E-4	0.0	0.002358819155818628	0.0
26-27	2.207082251058365E-4	5.793590909028207E-4	0.0	0.004338296049736599	0.0
28-29	2.276053571403939E-4	5.862562229373781E-4	0.0	0.014014972294220617	0.0
30-31	2.6209101731318083E-4	6.414332792138373E-4	0.0	0.04696946915533583	0.0
32-33	2.6898814934773823E-4	6.483304112483947E-4	0.0	0.13409404101586478	0.0
34-35	2.8967954545141043E-4	7.724787878704277E-4	0.0	0.25252469518124976	0.0
36-37	3.0347380952052517E-4	8.897300324579034E-4	0.0	0.42366323235472225	0.0
38-39	3.0347380952052517E-4	9.17318560596133E-4	0.0	0.6280735344628996	0.0
40-41	3.241652056241973E-4	0.0010276726731490511	0.0	0.8190689147638629	0.0
42-43	3.310623376587547E-4	0.0010552612012872807	0.0	1.063530862596715	0.0
44-45	3.379594696933121E-4	0.001144923917736527	0.0	1.2577472035578166	0.0
46-47	3.448566017278695E-4	0.0012138952380821007	0.0	1.4417419948437042	0.0
48-49	3.586508657969843E-4	0.001220792370116658	0.0	1.5697320740089855	0.0
50-51	3.724451298660991E-4	0.0013518378787732484	0.0	1.715020160316937	0.0
52-53	3.9313652596977125E-4	0.0013656321428423632	0.0	1.8642465090166207	0.0
54-55	4.0693079003888605E-4	0.0013656321428423632	0.0	2.001402876655829	0.0
56-57	4.1382792207344344E-4	0.001379426406911478	0.0	2.1101223689165574	0.0
58-59	4.1382792207344344E-4	0.0014001178030151502	0.0	2.1621060530610166	0.0
60-61	4.483135822462304E-4	0.0014415005952224946	0.0	2.1811421374763946	0.0
62-63	4.621078463153452E-4	0.0014690891233607242	0.0	2.192087886015237	0.0
64-65	4.690049783499025E-4	0.0016277231601555442	0.0	2.195764057389656	0.0
66-67	4.965935064881321E-4	0.0016484145562592164	0.0	2.1985780872597553	0.0
68-69	5.103877705572469E-4	0.0016897973484665606	0.0	2.2008472436991253	0.0
70-71	5.103877705572469E-4	0.0017242830086393475	0.0	2.203130194402563	0.0
72-73	5.241820346263617E-4	0.0017242830086393475	0.0	2.205302790993449	0.0
74-75	5.241820346263617E-4	0.0017380772727084623	0.0	2.207068456794296	0.0
76-77	5.310791666609191E-4	0.0018277399891577085	0.0	2.208530648785622	0.0
78-79	5.379762986954765E-4	0.0018622256493304953	0.0	2.209868692400326	0.0
80-81	5.379762986954765E-4	0.0018691227813650527	0.0	2.2103790801708834	0.0
82-83	5.379762986954765E-4	0.0018829170454341675	0.0	2.2108135994890605	0.0
84-85	5.448734307300339E-4	0.0018967113095032823	0.0	2.2109515421297514	0.0
86-87	5.517705627645913E-4	0.001924299837641512	0.0	2.21097913065789	0.0
88	5.517705627645913E-4	0.0020553453462981023	0.0	2.2109860277899243	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5540	0.0	33.4832	1
GTATCAA	8765	0.0	31.02974	1
TCAACGC	8950	0.0	28.212154	4
ATCAACG	9225	0.0	27.419523	3
CAACGCA	9325	0.0	26.982653	5
AACGCAG	9895	0.0	25.469376	6
TATCAAC	10690	0.0	24.02637	2
ACGCAGA	11985	0.0	21.027328	7
CGCAGAG	12125	0.0	20.74674	8
GTACATG	19540	0.0	19.362566	1
TACATGG	19305	0.0	18.836733	2
ACATGGG	19480	0.0	18.067825	3
CATGGGG	14315	0.0	16.037977	4
GCAGAGT	15975	0.0	15.800638	9
AGAGTAC	12870	0.0	15.230156	10-11
GTATAAG	3045	0.0	14.91013	1
GTGTAGC	3950	0.0	14.449613	1
CAGAGTA	15365	0.0	14.093237	10-11
GTATAGC	3080	0.0	13.898371	1
GTATAGG	2565	0.0	13.148816	1
>>END_MODULE