FastQCFastQC Report
Fri 27 May 2016
SRR2138834_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138834_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6985316
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91750.049.9161071
GTATCAA167100.039.3705521
ATCAACG206900.031.2393593
TCAACGC208050.031.0877044
CAACGCA213050.030.3581165
TATCAAC217750.030.0327842
AACGCAG219450.029.5923236
ACGCAGA251800.025.7036117
CGCAGAG258550.025.0663938
GTGGTAT45950.022.5612371
TGGTATC44700.021.8227712
GTACATG226100.021.2423171
GCAGAGT305150.021.2102539
TACATGG224200.020.8571362
GAGTACT166200.020.07664112-13
ACATGGG231650.019.5198483
AGAGTAC273850.019.2669110-11
CAGAGTA295000.018.5897310-11
GTACTTT186800.017.83959414-15
AGTACTT182200.017.56953212-13
Produced by FastQC (version 0.11.5)