##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138834_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6985316
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95367553880168	34.0	31.0	34.0	31.0	34.0
2	33.06110102964562	34.0	33.0	34.0	31.0	34.0
3	33.1215495476511	34.0	33.0	34.0	31.0	34.0
4	36.489543350651566	37.0	37.0	37.0	35.0	37.0
5	36.451732605940805	37.0	37.0	37.0	35.0	37.0
6	36.48315308856464	37.0	37.0	37.0	35.0	37.0
7	36.441264647154114	37.0	37.0	37.0	35.0	37.0
8	36.42594651408755	37.0	37.0	37.0	35.0	37.0
9	38.21663916134932	39.0	39.0	39.0	37.0	39.0
10-11	38.16878284389711	39.0	39.0	39.0	37.0	39.0
12-13	38.140166386173505	39.0	39.0	39.0	37.0	39.0
14-15	39.60919692108418	41.0	40.0	41.0	37.0	41.0
16-17	39.584375352525214	41.0	40.0	41.0	37.0	41.0
18-19	39.58555189199744	41.0	40.0	41.0	37.0	41.0
20-21	39.55719210826085	41.0	39.5	41.0	37.0	41.0
22-23	39.477532351904046	41.0	39.0	41.0	37.0	41.0
24-25	39.320768330829324	40.5	39.0	41.0	36.0	41.0
26-27	39.33184785820812	40.5	39.0	41.0	36.0	41.0
28-29	39.265406732380164	41.0	39.0	41.0	36.0	41.0
30-31	39.184209181078174	40.5	39.0	41.0	36.0	41.0
32-33	38.992579314464024	40.0	39.0	41.0	35.0	41.0
34-35	38.915611310508766	40.0	38.5	41.0	35.0	41.0
36-37	39.10944740997481	40.5	39.0	41.0	35.0	41.0
38-39	39.179925227925324	41.0	39.0	41.0	35.0	41.0
40-41	39.149670097502664	41.0	39.0	41.0	35.0	41.0
42-43	38.99923957955534	40.0	39.0	41.0	35.0	41.0
44-45	39.01338160315123	40.0	39.0	41.0	35.0	41.0
46-47	38.956026667373706	40.0	38.5	41.0	35.0	41.0
48-49	38.881284199504904	40.0	38.0	41.0	35.0	41.0
50-51	38.79902527142612	40.0	38.0	41.0	35.0	41.0
52-53	38.671814916357405	40.0	38.0	41.0	35.0	41.0
54-55	38.52388553990686	40.0	38.0	41.0	34.5	41.0
56-57	38.3653889524533	40.0	37.0	41.0	34.0	41.0
58-59	38.19004557847116	40.0	37.0	41.0	34.0	41.0
60-61	37.99176031994581	39.5	36.5	41.0	34.0	41.0
62-63	37.74733723512304	39.0	36.0	41.0	34.0	41.0
64-65	37.45821442644292	39.0	35.5	40.5	33.0	41.0
66-67	37.12198832981268	38.0	35.0	40.0	33.0	41.0
68-69	36.779203195871645	37.5	35.0	40.0	32.5	41.0
70-71	36.410204912719635	37.0	35.0	39.5	32.0	41.0
72-73	36.06525935725571	36.5	35.0	39.0	32.0	41.0
74-75	35.72480546133532	36.0	35.0	39.0	32.0	40.0
76-77	34.87915714664163	35.0	34.0	37.0	30.5	39.0
78-79	35.108392587281514	35.0	34.0	37.0	31.0	39.0
80-81	34.90101746799953	35.0	34.0	37.0	31.0	39.0
82-83	34.64735647221657	35.0	34.0	36.0	31.0	38.5
84-85	34.39822444512809	35.0	34.0	36.0	31.0	37.0
86-87	34.18923417289591	35.0	34.0	35.5	31.0	37.0
88-89	33.99920222336662	35.0	34.0	35.0	31.0	36.5
90-91	33.80844419233041	35.0	34.0	35.0	31.0	36.0
92-93	33.68250542819668	35.0	34.0	35.0	30.0	36.0
94-95	33.6111519063693	35.0	34.0	35.0	30.0	36.0
96-97	33.58598872908286	35.0	34.0	35.0	30.0	35.0
98-99	33.61436602343382	35.0	34.0	35.0	30.5	35.0
100-101	32.21184744232334	33.5	31.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	13.0
21	69.0
22	360.0
23	1406.0
24	3808.0
25	8259.0
26	15593.0
27	26435.0
28	40163.0
29	56819.0
30	76001.0
31	100182.0
32	130895.0
33	176949.0
34	259864.0
35	399596.0
36	740099.0
37	1632642.0
38	2253899.0
39	1010180.0
40	52083.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.05180524402905	20.973940191109463	15.65229117766469	24.3219633871968
2	13.639418979232728	21.278591083637295	41.21046852374581	23.871521413384166
3	17.00376904924559	26.13518128600052	30.7465832612297	26.114466403524194
4	11.988877811683823	18.57141466470522	38.53023972000694	30.909467803604016
5	13.409414835348896	37.67778007465947	35.21654567953691	13.696259410454731
6	30.197331087097563	37.74897227269317	18.614834318161126	13.438862322048136
7	26.826435171499067	32.5853307975374	22.6252502571466	17.96298377381693
8	24.890165352163045	36.49488001399516	20.657059502184094	17.957895131657704
9	25.527094419748053	17.119595560719432	21.060212939331244	36.29309708020127
10-11	24.261235998485965	26.95662587061201	28.41789118774297	20.36424694315905
12-13	25.15946307940829	24.378897103581284	28.66742177447663	21.794218042533796
14-15	22.22297858523944	25.746014921300546	26.432439412459118	25.598567081000894
16-17	21.819189386533704	28.93896187494493	27.054057480160697	22.187791258360665
18-19	21.857737001447035	28.053075909522203	28.870555032871813	21.21863205615895
20-21	22.690899543403837	27.06611410523386	28.854618487234234	21.388367864128067
22-23	22.4147769064664	26.99912272318749	28.68634329477396	21.899757075572154
24-25	22.200580914586084	27.198386555199416	28.669301807106862	21.93173072310764
26-27	22.116788190471688	27.306619224575346	28.63069332849172	21.945899256461246
28-29	22.025428909745358	27.31488158876536	28.722610613055295	21.937078888433987
30-31	22.242171385779038	27.208031488655443	28.640012704555645	21.90978442100987
32-33	22.171818370636963	27.290317611985632	28.551369675257178	21.98649434212023
34-35	22.22409291708538	27.367485523886177	28.561309308227536	21.84711225080091
36-37	22.20015497303859	27.311781056170865	28.50630832702055	21.98175564376999
38-39	22.091009280742462	27.369873839907193	28.591234048723894	21.94788283062645
40-41	22.307359612212974	27.26330776735604	28.471450030699124	21.957882589731863
42-43	22.28180574355115	27.33574716017859	28.27172242845817	22.110724667812086
44-45	22.299272238080693	27.4347635925226	28.08805804998421	22.177906119412498
46-47	22.359610702957845	27.498799256746054	27.830892868004693	22.310697172291412
48-49	22.3173370262546	27.606985822618263	27.768493762808028	22.30718338831911
50-51	22.33853846599388	27.693174673986586	27.64972304008389	22.318563819935648
52-53	22.45992679492769	27.606221408411653	27.544387630062488	22.38946416659817
54-55	22.44621507143866	27.55549943817425	27.58531666535348	22.41296882503361
56-57	22.43917532651026	27.67315501525817	27.52885134106639	22.358818317165184
58-59	22.42663147630493	27.710889403050714	27.52685161159065	22.3356275090537
60-61	22.492263102074226	27.6752699873618	27.5206874038423	22.311779506721678
62-63	22.495770166662354	27.733953237148786	27.431580456829035	22.338696139359822
64-65	22.473684318232227	27.68280325744764	27.436297152291328	22.4072152720288
66-67	22.510156635556253	27.67464676234325	27.36342104898057	22.451775553119926
68-69	22.460464042249008	27.684647505738763	27.399871833774654	22.455016618237575
70-71	22.468412471527785	27.58769296984293	27.422849284036943	22.521045274592336
72-73	22.479600285260346	27.595087593671998	27.37441296397372	22.550899157093937
74-75	22.413356541171723	27.69281850266969	27.362915431606165	22.530909524552417
76-77	22.528488572475382	27.61474022128536	27.31140362571956	22.5453675805197
78-79	22.475373967907135	27.64186204412261	27.27288109459095	22.6098828933793
80-81	22.471285523450724	27.700310035465	27.29434566474366	22.534058776340615
82-83	22.468772948661222	27.673412263722376	27.35029511371991	22.507519673896493
84-85	22.54779297581314	27.687021634155162	27.291301665638496	22.4738837243932
86-87	22.452880879645637	27.65042395638418	27.379091527062965	22.51760363690722
88-89	22.46161054461183	27.64657242285022	27.341431861290665	22.550385171247285
90-91	22.500932274579075	27.672231977083793	27.34585042503395	22.480985323303184
92-93	22.496955859564718	27.64665557707629	27.375710676239816	22.480677887119178
94-95	22.376228184608223	27.71579476915354	27.372281907125668	22.535695139112565
96-97	22.38187136087502	27.75986412078339	27.37833561462181	22.479928903719774
98-99	22.621834414723672	28.256858357811687	27.43450114905614	21.686806078408498
100-101	20.389552224373926	32.04696769781321	25.51272370541165	22.05075637240122
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2505.0
1	1999.0
2	2026.0
3	2700.0
4	3430.0
5	4810.0
6	6069.5
7	7675.0
8	9283.5
9	9656.5
10	8913.0
11	8313.5
12	9267.5
13	12418.0
14	19905.5
15	29839.0
16	37468.5
17	40954.0
18	40292.5
19	36888.5
20	32411.5
21	28395.0
22	26397.5
23	27832.5
24	32684.5
25	40865.5
26	51604.5
27	63586.5
28	77487.0
29	92938.5
30	107830.0
31	123313.5
32	139339.5
33	155118.5
34	170026.5
35	182743.5
36	196494.0
37	209049.0
38	220177.5
39	230880.0
40	240099.0
41	249254.5
42	256170.5
43	262692.5
44	268846.5
45	273271.0
46	277713.0
47	281484.5
48	282440.0
49	279823.0
50	275614.5
51	270032.0
52	260814.5
53	249723.0
54	238381.5
55	223673.5
56	206971.0
57	188510.5
58	167303.5
59	144555.5
60	121162.0
61	98683.5
62	78205.0
63	60338.0
64	44581.0
65	32658.0
66	24423.5
67	18367.0
68	13734.0
69	9788.0
70	6829.5
71	4828.5
72	3308.5
73	2175.5
74	1442.5
75	941.0
76	578.5
77	358.0
78	246.0
79	153.0
80	99.5
81	68.5
82	55.5
83	42.5
84	28.0
85	17.5
86	12.5
87	11.5
88	10.0
89	7.0
90	4.0
91	3.0
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.028917804148015636
3	0.0
4	0.0
5	0.0
6	0.0
7	1.4315744627730513E-5
8	6.155770189924121E-4
9	5.726297851092205E-4
10-11	0.0
12-13	0.0
14-15	0.0019183097801158888
16-17	2.147361694159577E-5
18-19	0.0
20-21	2.147403195421851E-4
22-23	0.001832547926676035
24-25	7.445731712817555E-4
26-27	0.01445113300678165
28-29	0.31192849049564114
30-31	0.3124915305933252
32-33	0.33013251184124914
34-35	0.21304471111288267
36-37	0.07130459995853494
38-39	0.06301131479165979
40-41	0.02497981083781601
42-43	0.009479203761523728
44-45	0.012541091046920996
46-47	0.008904054248340385
48-49	0.0058883143147764145
50-51	0.006537277279745534
52-53	0.0023408711404224207
54-55	8.305777582706304E-4
56-57	0.003044256278222633
58-59	0.0036736745868312347
60-61	0.002361644164237436
62-63	0.0016966740809499625
64-65	5.430833961224317E-4
66-67	0.007863861963221067
68-69	0.01133287192544935
70-71	0.011410519678358533
72-73	0.01006871533406976
74-75	0.010151717955710252
76-77	0.008541644962824096
78-79	0.0019823022707396924
80-81	0.0016560823765484683
82-83	0.0014172343969874321
84-85	0.0
86-87	0.0026444789161620334
88-89	0.0
90-91	0.007948350966929602
92-93	0.01671847680670356
94-95	0.0013734580576879314
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	75.0
20-21	307.0
22-23	642.0
24-25	1659.0
26-27	2456.0
28-29	4284.0
30-31	9796.0
32-33	12630.0
34-35	18498.0
36-37	26813.0
38-39	32058.0
40-41	35481.0
42-43	42380.0
44-45	51970.0
46-47	51904.0
48-49	45332.0
50-51	39548.0
52-53	37823.0
54-55	40605.0
56-57	41903.0
58-59	42589.0
60-61	41472.0
62-63	41764.0
64-65	44349.0
66-67	46642.0
68-69	47056.0
70-71	46293.0
72-73	45579.0
74-75	45703.0
76-77	46974.0
78-79	49987.0
80-81	50992.0
82-83	50815.0
84-85	52035.0
86-87	55331.0
88-89	60237.0
90-91	64244.0
92-93	68082.0
94-95	81771.0
96-97	139809.0
98-99	648366.0
100-101	4719062.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.96925967681601
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.42493522426919	78.64189672778734
2	6.985416581946849	12.15033017332144
3	1.580719765992627	4.1242208341466595
4	0.4917166791896401	1.7105694223946175
5	0.21654173702776264	0.9416237279218148
6	0.1084521220754597	0.5659200460369443
7	0.05984240741003595	0.3643114908810465
8	0.03460809032597177	0.24078719955825142
9	0.02251904646707288	0.1762618319882233
>10	0.07357344358945546	0.966947145119914
>50	0.0014140827526975682	0.08281599836810853
>100	2.6081895320277345E-4	0.03431540247564698
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.4315744627730513E-5	0.0
4	0.0	2.8631489255461026E-5	0.0	2.8631489255461026E-5	2.8631489255461026E-5
5	0.0	2.8631489255461026E-5	0.0	2.8631489255461026E-5	2.8631489255461026E-5
6	0.0	2.8631489255461026E-5	0.0	2.8631489255461026E-5	2.8631489255461026E-5
7	0.0	2.8631489255461026E-5	0.0	4.294723388319154E-5	2.8631489255461026E-5
8	0.0	4.294723388319154E-5	0.0	4.294723388319154E-5	4.294723388319154E-5
9	0.0	5.726297851092205E-5	0.0	7.157872313865257E-5	4.294723388319154E-5
10-11	0.0	5.726297851092205E-5	0.0	8.589446776638308E-5	4.294723388319154E-5
12-13	0.0	5.726297851092205E-5	0.0	1.002102123941136E-4	4.294723388319154E-5
14-15	0.0	1.2884170164957463E-4	0.0	1.3599957396343986E-4	4.294723388319154E-5
16-17	0.0	1.2884170164957463E-4	0.0	1.5747319090503564E-4	4.294723388319154E-5
18-19	0.0	1.3599957396343988E-4	0.0	1.861046801604967E-4	5.01051061970568E-5
20-21	0.0	1.4315744627730513E-4	0.0	2.0757829710209245E-4	6.442085082478732E-5
22-23	0.0	1.7178893553276614E-4	0.0	2.50525530985284E-4	1.002102123941136E-4
24-25	0.0	2.004204247882272E-4	0.0	2.9347276486847555E-4	1.2884170164957463E-4
26-27	1.4315744627730513E-5	2.147361694159577E-4	0.0	4.008408495764544E-4	1.2884170164957463E-4
28-29	1.4315744627730513E-5	2.147361694159577E-4	0.0	0.0011738910594739022	1.2884170164957463E-4
30-31	1.4315744627730513E-5	2.6484127561301454E-4	0.0	0.0026484127561301448	1.2884170164957463E-4
32-33	1.4315744627730513E-5	2.7199914792687977E-4	0.0	0.005654719127953553	1.5747319090503564E-4
34-35	1.4315744627730513E-5	3.149463818100713E-4	0.0	0.011824805062505404	1.7178893553276617E-4
36-37	1.4315744627730513E-5	3.650514880071281E-4	0.0	0.019111519078020237	1.7178893553276617E-4
38-39	1.4315744627730513E-5	4.008408495764544E-4	0.0	0.031251270522335714	1.8610468016049667E-4
40-41	1.4315744627730513E-5	4.151565942041849E-4	0.0	0.04200239473776132	1.8610468016049667E-4
42-43	1.4315744627730513E-5	4.437880834596459E-4	0.0	0.05359814788622304	1.9326255247436192E-4
44-45	1.4315744627730513E-5	5.153668065982985E-4	0.0	0.06325411763762727	2.004204247882272E-4
46-47	2.1473616941595767E-5	5.869455297369511E-4	0.0	0.07318924440927224	2.004204247882272E-4
48-49	2.8631489255461026E-5	5.869455297369511E-4	0.0	0.08128479799625385	2.004204247882272E-4
50-51	2.8631489255461026E-5	6.298927636201425E-4	0.0	0.08786288265269604	2.004204247882272E-4
52-53	2.8631489255461026E-5	6.298927636201425E-4	0.0	0.09368223284386848	2.2189404172982298E-4
54-55	2.8631489255461026E-5	6.298927636201425E-4	0.0	0.09912937367471994	2.4336765867141873E-4
56-57	2.8631489255461026E-5	6.442085082478731E-4	0.0	0.10379630642336009	2.4336765867141873E-4
58-59	2.8631489255461026E-5	6.585242528756037E-4	0.0	0.10847755491662797	2.4336765867141873E-4
60-61	2.8631489255461026E-5	6.585242528756037E-4	0.0	0.11306575106981559	2.4336765867141873E-4
62-63	2.8631489255461026E-5	6.585242528756037E-4	0.0	0.11743921105358726	2.4336765867141873E-4
64-65	2.8631489255461026E-5	7.730502098974477E-4	0.0	0.1215549876340598	2.4336765867141873E-4
66-67	2.8631489255461026E-5	7.873659545251782E-4	0.0	0.1261718152765029	2.50525530985284E-4
68-69	2.8631489255461026E-5	8.088395714667741E-4	0.0	0.13062401185572708	2.7199914792687977E-4
70-71	2.8631489255461026E-5	8.231553160945045E-4	0.0	0.13466820971306093	2.8631489255461027E-4
72-73	2.8631489255461026E-5	8.303131884083698E-4	0.0	0.13777472629727847	3.149463818100713E-4
74-75	2.8631489255461026E-5	8.446289330361002E-4	0.0	0.1406092437335691	3.149463818100713E-4
76-77	2.8631489255461026E-5	8.732604222915614E-4	0.0	0.14313597266036354	3.4357787106553233E-4
78-79	2.8631489255461026E-5	9.018919115470224E-4	0.0	0.14517596626981516	3.5073574337939756E-4
80-81	2.8631489255461026E-5	9.018919115470224E-4	0.0	0.14612080541524536	3.5789361569326284E-4
82-83	2.8631489255461026E-5	9.018919115470224E-4	0.0	0.14645722541399703	3.8652510494872384E-4
84-85	2.8631489255461026E-5	9.305234008024834E-4	0.0	0.14661469860490206	4.008408495764544E-4
86-87	2.8631489255461026E-5	9.448391454302139E-4	0.0	0.1466862773280407	4.151565942041849E-4
88-89	2.8631489255461026E-5	0.001030733613196597	0.0	0.1467506981788655	4.294723388319154E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9175	0.0	49.916107	1
GTATCAA	16710	0.0	39.370552	1
ATCAACG	20690	0.0	31.239359	3
TCAACGC	20805	0.0	31.087704	4
CAACGCA	21305	0.0	30.358116	5
TATCAAC	21775	0.0	30.032784	2
AACGCAG	21945	0.0	29.592323	6
ACGCAGA	25180	0.0	25.703611	7
CGCAGAG	25855	0.0	25.066393	8
GTGGTAT	4595	0.0	22.561237	1
TGGTATC	4470	0.0	21.822771	2
GTACATG	22610	0.0	21.242317	1
GCAGAGT	30515	0.0	21.210253	9
TACATGG	22420	0.0	20.857136	2
GAGTACT	16620	0.0	20.076641	12-13
ACATGGG	23165	0.0	19.519848	3
AGAGTAC	27385	0.0	19.26691	10-11
CAGAGTA	29500	0.0	18.58973	10-11
GTACTTT	18680	0.0	17.839594	14-15
AGTACTT	18220	0.0	17.569532	12-13
>>END_MODULE