FastQCFastQC Report
Fri 27 May 2016
SRR2138833_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138833_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7681056
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100050.051.1777271
GTATCAA176900.037.9481661
ATCAACG209800.031.4508823
TCAACGC210300.031.417834
CAACGCA215150.030.6666185
TATCAAC221200.030.1498872
AACGCAG221250.029.8405596
ACGCAGA253100.026.0854347
CGCAGAG256100.025.7627378
GTGGTAT48950.022.8567621
GCAGAGT304950.021.664579
TGGTATC48400.021.394092
GAGTACT181600.020.001512-13
CAGAGTA296300.019.63191810-11
GTACATG213250.019.6284751
TACATGG208050.019.1278782
AGAGTAC274300.019.0074710-11
ACATGGG210900.018.1397113
GTACTTT202350.017.88575614-15
CTTATAC42950.017.1622141
Produced by FastQC (version 0.11.5)