##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138833_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7681056
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.448573998158587	32.0	28.0	33.0	18.0	34.0
2	30.547760620414692	32.0	30.0	33.0	25.0	33.0
3	31.772377652239484	33.0	31.0	33.0	29.0	34.0
4	32.312120625080716	33.0	33.0	33.0	31.0	34.0
5	32.831571726595925	33.0	33.0	34.0	32.0	34.0
6	37.03489975857486	38.0	37.0	38.0	36.0	38.0
7	37.13285855486537	38.0	38.0	38.0	36.0	38.0
8	37.30704345860777	38.0	38.0	38.0	36.0	38.0
9	37.34547411189295	38.0	38.0	38.0	37.0	38.0
10-11	37.40758907368986	38.0	38.0	38.0	37.0	38.0
12-13	37.440819270162855	38.0	38.0	38.0	37.0	38.0
14-15	37.435001580511845	38.0	38.0	38.0	37.0	38.0
16-17	37.391801075268816	38.0	38.0	38.0	37.0	38.0
18-19	37.425710800702404	38.0	38.0	38.0	37.0	38.0
20-21	37.45695853578885	38.0	38.0	38.0	37.0	38.0
22-23	37.46629776870989	38.0	38.0	38.0	37.0	38.0
24-25	37.466223724146026	38.0	38.0	38.0	37.0	38.0
26-27	37.436051679512744	38.0	38.0	38.0	37.0	38.0
28-29	37.40541856463584	38.0	38.0	38.0	37.0	38.0
30-31	37.31336714818174	38.0	38.0	38.0	37.0	38.0
32-33	37.37497876503447	38.0	38.0	38.0	37.0	38.0
34-35	37.3824545811421	38.0	38.0	38.0	37.0	38.0
36-37	37.364532394295566	38.0	38.0	38.0	37.0	38.0
38-39	37.34438214137645	38.0	38.0	38.0	37.0	38.0
40-41	37.334350885342296	38.0	38.0	38.0	37.0	38.0
42-43	37.31707346279062	38.0	38.0	38.0	37.0	38.0
44-45	37.282829932248276	38.0	38.0	38.0	37.0	38.0
46-47	37.25632299687706	38.0	38.0	38.0	37.0	38.0
48-49	37.227032449347675	38.0	38.0	38.0	37.0	38.0
50-51	37.18577103572201	38.0	38.0	38.0	37.0	38.0
52-53	37.167487423978805	38.0	38.0	38.0	37.0	38.0
54-55	37.15787478329083	38.0	38.0	38.0	37.0	38.0
56-57	37.15245276566709	38.0	38.0	38.0	37.0	38.0
58-59	37.1463359596397	38.0	38.0	38.0	36.5	38.0
60-61	37.147277772946	38.0	38.0	38.0	36.0	38.0
62-63	37.160136605639025	38.0	38.0	38.0	36.5	38.0
64-65	37.16459872335578	38.0	38.0	38.0	36.5	38.0
66-67	37.150075273904605	38.0	38.0	38.0	36.0	38.0
68-69	37.152778188007154	38.0	38.0	38.0	36.0	38.0
70-71	37.13663929790522	38.0	38.0	38.0	36.0	38.0
72-73	37.131047205941094	38.0	38.0	38.0	36.0	38.0
74-75	37.12284928376329	38.0	38.0	38.0	36.0	38.0
76-77	37.12604109541972	38.0	38.0	38.0	36.0	38.0
78-79	37.11209564965257	38.0	38.0	38.0	36.0	38.0
80-81	37.11027690064324	38.0	38.0	38.0	36.0	38.0
82-83	37.06302708663624	38.0	38.0	38.0	36.0	38.0
84-85	37.05432418835305	38.0	38.0	38.0	36.0	38.0
86-87	37.04854833298071	38.0	38.0	38.0	36.0	38.0
88-89	37.053997037770074	38.0	38.0	38.0	36.0	38.0
90-91	37.04418446926363	38.0	38.0	38.0	36.0	38.0
92-93	37.028769720476014	38.0	38.0	38.0	36.0	38.0
94-95	37.004218343019815	38.0	38.0	38.0	36.0	38.0
96-97	36.976080043344275	38.0	38.0	38.0	36.0	38.0
98-99	36.9830661594975	38.0	38.0	38.0	36.0	38.0
100	35.53483617958287	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	11.0
19	32.0
20	70.0
21	239.0
22	568.0
23	1388.0
24	3067.0
25	6277.0
26	11067.0
27	17935.0
28	28140.0
29	40861.0
30	56664.0
31	74724.0
32	97198.0
33	128520.0
34	189407.0
35	342108.0
36	1006247.0
37	5676531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.81412764078273	20.947497323284715	14.883409260393362	23.354965775539196
2	14.410834110527187	20.845582572490606	42.170019851807375	22.573563465174836
3	17.732001953898006	26.463887257168807	30.53418696595885	25.269923822974338
4	12.27794206984043	18.548709855241	39.564465315048004	29.608882759870564
5	13.235166412940838	37.678956127716376	35.9059131766834	13.179964282659384
6	29.283955226989622	38.370414172217984	19.18249261559869	13.163137985193702
7	26.26155830656618	32.938986514354276	23.209790424649945	17.5896647544296
8	24.118181145925767	36.81810157353364	21.376995558943978	17.686721721596612
9	25.08665819819428	17.522343005939693	21.697155814467287	35.69384298139874
10-11	23.80947204304848	27.137105391481814	29.11604319976222	19.937379365707486
12-13	24.799000553765183	24.58264351367947	29.216057406708597	21.402298525846746
14-15	21.9198506038753	26.019924864497803	26.9756788649894	25.084545666637503
16-17	21.450149562768452	29.23280991571992	27.59852942095462	21.718511100557006
18-19	21.391798211079312	28.293967652364465	29.453411093474646	20.860823043081574
20-21	22.244361330028546	27.363995834905396	29.419814768379755	20.9718280666863
22-23	21.941751971702477	27.335121197728533	29.26197082755259	21.461156003016402
24-25	21.699868330466117	27.451115447652004	29.34782728993501	21.50118893194687
26-27	21.720856340758623	27.593258648329783	29.240528556118218	21.445356454793377
28-29	21.615928810484096	27.636546104967287	29.281077845949255	21.46644723859937
30-31	21.93117767139319	27.497355827336843	29.16601467963068	21.40545182163929
32-33	21.772353961752156	27.542507303584717	29.173073992581717	21.512064742081407
34-35	21.789751863715967	27.580001694762764	29.175131762574196	21.45511467894707
36-37	21.75645047374242	27.56180471903942	29.129087367559443	21.55265743965872
38-39	21.63116084866345	27.60698818755078	29.24851834149052	21.513332622295255
40-41	21.882062443007747	27.509808553646064	29.130895085577997	21.477233917768196
42-43	21.816826280314334	27.54410786249533	28.996988715089874	21.64207714210046
44-45	21.913320255918244	27.619741195461035	28.85853675484123	21.608401793779496
46-47	21.911399685181735	27.59657321615927	28.625471768464607	21.866555330194384
48-49	21.8647258254308	27.675129876901238	28.672704118592087	21.78744017907587
50-51	21.835511173945502	27.861166311300206	28.52049175557295	21.782830759181344
52-53	21.97963849834404	27.73713246063237	28.48886246829209	21.794366572731498
54-55	21.96168390664492	27.70978598151798	28.48277939096167	21.845750720875426
56-57	22.030918880929086	27.825027509384952	28.362696630426456	21.781356979259506
58-59	21.92868043468833	27.90487578200316	28.464431233946357	21.70201254936215
60-61	21.982273363134723	27.959511063409842	28.38074854716659	21.677467026288845
62-63	21.91540994667913	28.031163533186525	28.328317957346826	21.72510856278752
64-65	21.89957830616419	27.964550598123992	28.351430941515105	21.78444015419671
66-67	21.99731004750662	27.92517360161651	28.220286902121767	21.85722944875511
68-69	21.991238694490377	27.89263053340746	28.264518967164527	21.851611804937637
70-71	21.949743363103174	27.7192071877999	28.341666632922756	21.989382816174167
72-73	22.037030077542685	27.69094271012227	28.272410890877104	21.999616321457943
74-75	21.963355899168064	27.81242612593693	28.254411552886406	21.9698064220086
76-77	22.073823134179985	27.76640146248994	28.128578716359655	22.031196686970418
78-79	22.02314330480807	27.80592423741069	28.11668284001826	22.054249617762984
80-81	22.022537609677283	27.811528214419322	28.14868438117083	22.017249794732557
82-83	22.076653260582408	27.75539915960691	28.19044672655474	21.977500853255943
84-85	22.21506162639899	27.723627719738708	28.11221028489156	21.949100368970736
86-87	22.056119587791258	27.759310246086432	28.173120160795236	22.011450005327074
88-89	22.147057573398392	27.766953532024107	28.106866191134312	21.979122703443192
90-91	22.15513313943459	27.78498454395133	28.102924493152788	21.95695782346129
92-93	22.181642707435273	27.804651370327	28.05376840314539	21.959937519092342
94-95	22.082282600157402	27.9037250976139	28.04492193443362	21.969070367795073
96-97	22.210273852026894	27.967116450349945	28.0919214500024	21.730688247620762
98-99	23.286956852678315	29.894803899265597	28.330559841843268	18.487679406212816
100	22.1118364722161	30.874991419704696	22.347099137635162	24.666072970444045
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1080.0
1	1017.5
2	1495.0
3	2285.5
4	2909.5
5	3822.5
6	4733.0
7	6207.0
8	8389.0
9	9963.0
10	10283.5
11	10426.5
12	11564.0
13	13867.0
14	18158.5
15	23057.0
16	26564.5
17	28372.0
18	27895.5
19	25688.0
20	23635.0
21	22719.0
22	24011.0
23	28541.0
24	36265.5
25	47230.5
26	61700.0
27	78136.5
28	94372.5
29	112456.0
30	131224.0
31	148940.5
32	167681.0
33	186128.5
34	204338.0
35	221248.0
36	237664.0
37	253776.0
38	265341.0
39	273954.0
40	283888.0
41	293691.5
42	301206.5
43	310097.5
44	315858.0
45	318705.0
46	319878.0
47	316600.0
48	311456.0
49	304154.0
50	295327.0
51	282771.0
52	267895.5
53	252104.5
54	235408.0
55	217095.0
56	197740.5
57	176924.0
58	154767.0
59	133212.5
60	110049.5
61	87738.0
62	68347.0
63	52072.5
64	39004.0
65	28539.5
66	20783.0
67	15703.5
68	11954.5
69	8530.0
70	5925.5
71	4257.5
72	3014.0
73	2112.5
74	1438.5
75	924.5
76	564.5
77	307.0
78	178.0
79	120.0
80	80.5
81	54.0
82	32.0
83	17.5
84	14.5
85	12.0
86	10.0
87	8.0
88	5.5
89	4.0
90	2.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008423320960034663
3	0.0
4	0.0037755225323184726
5	0.002512675340474018
6	0.0
7	0.0
8	0.0
9	2.2132373465315187E-4
10-11	5.402902934179884E-4
12-13	3.254760803722822E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.137242603555689E-4
24-25	0.0016210226191897567
26-27	0.0
28-29	0.002038373122962807
30-31	0.003212854127559563
32-33	6.982938527560614E-4
34-35	1.961530592783045E-5
36-37	0.001212961006319658
38-39	0.0021659857417569597
40-41	0.0017062663756931377
42-43	0.0
44-45	0.0
46-47	0.001620529976509712
48-49	4.939868937833441E-4
50-51	0.0018991544882534233
52-53	0.0028196180813621326
54-55	0.0021192156507653707
56-57	0.001986047840916876
58-59	0.0021857429002511168
60-61	9.663404707380551E-4
62-63	7.324919114228522E-4
64-65	0.0035492349733821545
66-67	0.002573948616855556
68-69	1.2923745378953292E-4
70-71	0.0031742370533246514
72-73	0.010115330055791617
74-75	0.012012655578181793
76-77	0.0050163603428682296
78-79	0.004523134608040306
80-81	3.440433530525022E-4
82-83	1.0543310337369363E-4
84-85	5.3066380128429733E-5
86-87	0.0016637291925097996
88-89	0.004304063320149834
90-91	6.426429193332154E-4
92-93	0.0011388969019040015
94-95	0.0026261906216531305
96-97	0.002332823721489192
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	42.0
20-21	195.0
22-23	391.0
24-25	524.0
26-27	1482.0
28-29	3718.0
30-31	10087.0
32-33	13397.0
34-35	18688.0
36-37	29109.0
38-39	34683.0
40-41	34201.0
42-43	39419.0
44-45	47139.0
46-47	47756.0
48-49	44636.0
50-51	40018.0
52-53	38663.0
54-55	41070.0
56-57	42284.0
58-59	42656.0
60-61	41771.0
62-63	40782.0
64-65	43727.0
66-67	48127.0
68-69	49148.0
70-71	50180.0
72-73	44367.0
74-75	43784.0
76-77	45288.0
78-79	46636.0
80-81	46728.0
82-83	44035.0
84-85	43505.0
86-87	45715.0
88-89	47254.0
90-91	48056.0
92-93	48988.0
94-95	61002.0
96-97	232699.0
98-99	543166.0
100-101	5535940.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.81327897526374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.37012498933031	74.06589938809665
2	8.053747005954543	13.500218892125263
3	2.065888096025626	5.194465659716166
4	0.7352168916914421	2.464837538026444
5	0.3188851124569726	1.3363403445707296
6	0.17124750188196255	0.8611688789429957
7	0.09593295105031092	0.5628318632510021
8	0.05235024345509374	0.3510116447299797
9	0.03901134331383376	0.2942701740325925
>10	0.09581638814652815	1.1899557586996443
>50	0.0013665262781778933	0.07506191951806519
>100	3.3859955291958737E-4	0.0502008746823379
>500	4.956725978900405E-5	0.027585178646093705
>1k	2.4783602531875254E-5	0.026151884962072866
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.603808642978257E-5	0.0	0.0	0.0
3	0.0	2.603808642978257E-5	0.0	0.0	0.0
4	0.0	3.905712964467386E-5	0.0	0.0	0.0
5	0.0	3.905712964467386E-5	0.0	0.0	0.0
6	1.0415234571913029E-4	3.905712964467386E-5	0.0	0.0	0.0
7	1.0415234571913029E-4	5.207617285956514E-5	0.0	0.0	0.0
8	1.0415234571913029E-4	6.509521607445642E-5	0.0	0.0	0.0
9	1.0415234571913029E-4	1.9528564822336928E-4	0.0	0.0	0.0
10-11	1.0415234571913029E-4	1.9528564822336928E-4	0.0	1.3019043214891286E-5	0.0
12-13	1.0415234571913029E-4	1.9528564822336928E-4	0.0	2.603808642978257E-5	0.0
14-15	1.1066186732657592E-4	2.7990942912016264E-4	0.0	2.603808642978257E-5	1.3019043214891286E-5
16-17	1.6924756179358672E-4	2.994379939424996E-4	0.0	2.603808642978257E-5	2.603808642978257E-5
18-19	1.6924756179358672E-4	3.450046451946191E-4	0.0	3.905712964467386E-5	3.905712964467386E-5
20-21	1.7575708340103236E-4	3.775522532318473E-4	0.0	5.207617285956514E-5	3.905712964467386E-5
22-23	1.82266605008478E-4	4.035903396616299E-4	0.0	5.858569446701078E-5	4.55666512521195E-5
24-25	1.8877612661592362E-4	4.3613794769885804E-4	0.0	6.509521607445642E-5	5.207617285956514E-5
26-27	1.9528564822336928E-4	4.6868555573608626E-4	0.0	9.1133302504239E-5	5.207617285956514E-5
28-29	1.9528564822336928E-4	4.7519507734353195E-4	0.0	3.450046451946191E-4	5.207617285956514E-5
30-31	2.0179516983081494E-4	5.402902934179884E-4	0.0	0.0010675615436210855	5.207617285956514E-5
32-33	2.148142130457062E-4	5.598188582403253E-4	0.0	0.002388994429932551	6.509521607445642E-5
34-35	2.278332562605975E-4	6.444426391371186E-4	0.0	0.004087979569475863	6.509521607445642E-5
36-37	2.3434277786804313E-4	7.420854632488032E-4	0.0	0.006457445434586078	7.160473768190207E-5
38-39	2.3434277786804313E-4	7.616140280711402E-4	0.0	0.010050701361896073	8.462378089679336E-5
40-41	2.5387134269038006E-4	8.332187657530423E-4	0.0	0.014158209496194275	9.1133302504239E-5
42-43	2.73399907512717E-4	8.462378089679336E-4	0.0	0.01814203671995101	9.1133302504239E-5
44-45	2.929284723350539E-4	9.373711114721725E-4	0.0	0.02285493036374165	9.1133302504239E-5
46-47	3.1245703715739086E-4	0.0010350139355838571	0.0	0.02805603812809072	9.764282411168464E-5
48-49	3.254760803722822E-4	0.0010675615436210855	0.0	0.03334827919494403	1.1717138893402157E-4
50-51	3.710427316244016E-4	0.0012433186270221178	0.0	0.038315044181425055	1.1717138893402157E-4
52-53	3.9057129644673856E-4	0.0012628471918444547	0.0	0.043548699553811354	1.236809105414672E-4
54-55	3.9057129644673856E-4	0.0012888852782742372	0.0	0.04835272650010623	1.3019043214891284E-4
56-57	3.9057129644673856E-4	0.0013279424079189112	0.0	0.05208919190278004	1.3019043214891284E-4
58-59	3.9057129644673856E-4	0.0013279424079189112	0.0	0.055793109697416604	1.3019043214891284E-4
60-61	4.035903396616299E-4	0.0013604900159561394	0.0	0.05969882266188399	1.3019043214891284E-4
62-63	4.1660938287652115E-4	0.0013930376239933676	0.0	0.0632660405027642	1.3019043214891284E-4
64-65	4.1660938287652115E-4	0.001549266142572063	0.0	0.06689184403811142	1.3019043214891284E-4
66-67	4.231189044839668E-4	0.001588323272216737	0.0	0.07055019518149588	1.366999537563585E-4
68-69	4.296284260914124E-4	0.0016859660963284215	0.0	0.07375938933396659	1.4320947536380414E-4
70-71	4.7519507734353195E-4	0.0017250232259730953	0.0	0.07661055979802778	1.4320947536380414E-4
72-73	5.402902934179884E-4	0.001731532747580541	0.0	0.07906464944403477	1.5622851857869543E-4
74-75	5.46799815025434E-4	0.001751061312402878	0.0	0.0811932630096695	1.5622851857869543E-4
76-77	5.46799815025434E-4	0.0017901184420475518	0.0	0.08309404331904363	1.5622851857869543E-4
78-79	5.46799815025434E-4	0.0018096470068698887	0.0	0.08432434290285086	1.5622851857869543E-4
80-81	5.46799815025434E-4	0.0018096470068698887	0.0	0.08480604750180185	1.5622851857869543E-4
82-83	5.533093366328797E-4	0.00182266605008478	0.0	0.0851705807118188	1.5622851857869543E-4
84-85	5.728379014552165E-4	0.0018356850932996713	0.0	0.0853007711439677	1.5622851857869543E-4
86-87	5.858569446701078E-4	0.0018617231797294538	0.0	0.08532029970879004	1.5622851857869543E-4
88	5.988759878849992E-4	0.0019268183958039103	0.0	0.08532680923039748	1.5622851857869543E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10005	0.0	51.177727	1
GTATCAA	17690	0.0	37.948166	1
ATCAACG	20980	0.0	31.450882	3
TCAACGC	21030	0.0	31.41783	4
CAACGCA	21515	0.0	30.666618	5
TATCAAC	22120	0.0	30.149887	2
AACGCAG	22125	0.0	29.840559	6
ACGCAGA	25310	0.0	26.085434	7
CGCAGAG	25610	0.0	25.762737	8
GTGGTAT	4895	0.0	22.856762	1
GCAGAGT	30495	0.0	21.66457	9
TGGTATC	4840	0.0	21.39409	2
GAGTACT	18160	0.0	20.0015	12-13
CAGAGTA	29630	0.0	19.631918	10-11
GTACATG	21325	0.0	19.628475	1
TACATGG	20805	0.0	19.127878	2
AGAGTAC	27430	0.0	19.00747	10-11
ACATGGG	21090	0.0	18.139711	3
GTACTTT	20235	0.0	17.885756	14-15
CTTATAC	4295	0.0	17.162214	1
>>END_MODULE