FastQCFastQC Report
Fri 27 May 2016
SRR2138832_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138832_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6616810
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85750.049.563861
CTTATAC60250.038.901871
GTATCAA160000.038.477281
TCAACGC196600.031.40164
ATCAACG196350.031.375153
CAACGCA201150.030.6686045
TATCAAC205000.030.3691882
TATACAC88450.029.97583
AACGCAG207300.029.7574046
TTATACA94400.026.664562
ACGCAGA236650.026.04857
CGCAGAG238150.025.9026228
ACATCTC104550.023.3689618
ACACATC107800.022.7046096
TGGTATC41700.022.6490882
TACACAT121750.022.238995
GCAGAGT282350.021.9244529
GTGGTAT44250.021.1480711
GAGTACT167150.020.18887512-13
CACATCT119250.020.0886657
Produced by FastQC (version 0.11.5)