FastQCFastQC Report
Fri 27 May 2016
SRR2138831_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138831_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8376093
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73700.027.9684621
GTATCAA108550.026.0120371
TCAACGC104650.025.0675224
ATCAACG107600.024.3811363
CAACGCA110050.023.7582555
AACGCAG113800.022.9728896
TATCAAC125450.021.4893722
GTACATG228100.019.0816651
ACGCAGA139650.018.719927
TACATGG221150.018.6991982
CGCAGAG141450.018.4797178
ACATGGG224000.018.0237773
TATACCG5700.017.1826115
CATGGGG162900.016.1038444
GTGTAGC48350.014.2695161
AGAGTAC144400.014.077810-11
GCAGAGT188150.013.8249749
TATACTG52100.013.7311765
GTATATA85700.013.2684771
CAGAGTA174750.013.1067110-11
Produced by FastQC (version 0.11.5)