FastQCFastQC Report
Fri 27 May 2016
SRR2138830_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138830_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15644069
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA235700.020.361671
GTACATG385000.019.9070421
GGTATCA178550.019.8409791
TCAACGC225650.019.613454
TACATGG376500.019.5135062
ATCAACG227500.019.511693
CAACGCA231600.019.0895795
AACGCAG240500.018.4521686
ACATGGG388900.018.3727023
TATCAAC269900.017.0823842
CATGGGG280100.016.3671934
CGCAGAG275450.016.0467838
ACGCAGA277550.015.957597
AGAGTAC247800.013.97888310-11
TAGCCCT101350.013.4135914
TATACTG96400.013.3895425
GTATAGA79800.013.1675841
GTGTAGC96350.013.0869531
GAGTACA279500.012.7975591
GTATAGG51700.012.7753581
Produced by FastQC (version 0.11.5)