##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138830_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15644069
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.258570324638686	31.0	31.0	34.0	28.0	34.0
2	31.587738906035252	31.0	31.0	34.0	29.0	34.0
3	31.69020809100241	33.0	31.0	34.0	29.0	34.0
4	35.298776999769046	37.0	35.0	37.0	33.0	37.0
5	35.26437546395378	37.0	35.0	37.0	32.0	37.0
6	35.42067322766219	37.0	35.0	37.0	33.0	37.0
7	35.34603637966568	37.0	35.0	37.0	32.0	37.0
8	35.38910260495527	37.0	35.0	37.0	33.0	37.0
9	36.993403889998184	39.0	37.0	39.0	33.0	39.0
10-11	36.99873840367235	39.0	37.0	39.0	33.0	39.0
12-13	36.94337710348887	39.0	37.0	39.0	33.0	39.0
14-15	38.29767664665759	40.0	38.0	41.0	33.0	41.0
16-17	38.289325973952174	40.0	38.0	41.0	33.0	41.0
18-19	38.29974567358403	40.0	38.0	41.0	33.0	41.0
20-21	38.35959338032678	40.0	38.0	41.0	33.0	41.0
22-23	38.331514489752294	40.0	38.0	41.0	33.0	41.0
24-25	38.360548713272486	40.0	38.0	41.0	33.0	41.0
26-27	38.34919241345135	40.0	38.0	41.0	33.0	41.0
28-29	38.28966439027023	40.0	38.0	41.0	33.0	41.0
30-31	38.22416062041415	40.0	38.0	41.0	33.0	41.0
32-33	38.1941083592923	40.0	38.0	41.0	33.0	41.0
34-35	38.125909075346506	40.0	38.0	41.0	32.5	41.0
36-37	38.038294215251895	40.0	38.0	41.0	32.0	41.0
38-39	37.95286915654492	40.0	38.0	41.0	32.0	41.0
40-41	37.91428426488476	40.0	37.0	41.0	32.0	41.0
42-43	37.75135033759372	40.0	37.0	41.0	31.5	41.0
44-45	37.53764625398313	40.0	37.0	41.0	31.0	41.0
46-47	37.389324534497234	40.0	36.5	41.0	31.0	41.0
48-49	37.26162037623568	39.0	36.0	41.0	31.0	41.0
50-51	37.133979955450826	39.0	36.0	40.0	30.5	41.0
52-53	36.995716208616464	39.0	36.0	40.0	30.0	41.0
54-55	36.831819599951224	39.0	35.0	40.0	30.0	41.0
56-57	36.81922460635215	39.0	35.0	40.0	30.0	41.0
58-59	36.89676072228313	39.0	35.0	40.0	30.0	41.0
60-61	37.199841367744035	39.0	35.5	41.0	31.0	41.0
62-63	37.173105466132526	39.0	35.0	41.0	31.0	41.0
64-65	36.968665361869235	39.0	35.0	41.0	31.0	41.0
66-67	36.76043350488313	38.0	35.0	40.0	31.0	41.0
68-69	36.48280959087354	38.0	35.0	40.0	31.0	41.0
70-71	36.23447304763593	37.0	35.0	40.0	31.0	41.0
72-73	35.93462786690358	37.0	34.5	39.0	30.0	41.0
74-75	35.62493606660996	36.0	34.0	39.0	30.0	41.0
76-77	35.31477454765168	36.0	34.0	39.0	30.0	40.0
78-79	34.9986393782394	35.0	34.0	37.5	30.0	39.5
80-81	34.68766319787291	35.0	34.0	37.0	30.0	39.0
82-83	34.42411231832305	35.0	34.0	37.0	30.0	39.0
84-85	34.18203464975316	35.0	34.0	36.0	30.0	37.5
86-87	33.97294734828009	35.0	34.0	36.0	30.0	37.0
88-89	33.815896415900966	35.0	34.0	35.5	30.0	37.0
90-91	33.67998741865402	35.0	34.0	35.0	29.5	36.0
92-93	33.600358065105766	35.0	34.0	35.0	29.5	36.0
94-95	33.56947139438974	35.0	34.0	35.0	30.0	36.0
96-97	33.56942968547118	35.0	34.0	35.0	30.0	36.0
98-99	33.60648223963002	35.0	34.0	35.0	30.0	36.0
100	32.88616610511872	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	4.0
14	7.0
15	23.0
16	55.0
17	427.0
18	2394.0
19	8368.0
20	21016.0
21	41013.0
22	64610.0
23	89870.0
24	117641.0
25	146197.0
26	167204.0
27	175704.0
28	194548.0
29	233355.0
30	274910.0
31	324442.0
32	408361.0
33	532040.0
34	723249.0
35	1023427.0
36	1616149.0
37	2907130.0
38	4152782.0
39	2332214.0
40	86926.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.40450090062886	20.86622732231621	14.807682067881444	23.92158970917349
2	13.762426071042505	21.134055648008374	42.33570403369396	22.767814247255167
3	17.82494213945314	26.115475067713096	30.527709797749104	25.531872995084658
4	12.027570370674411	18.389504754097512	40.04373338795851	29.539191487269566
5	13.16320977256758	38.15115752992347	35.94536538289053	12.740267314618418
6	29.204116107111766	38.347187071722324	19.640200239523836	12.808496581642077
7	26.113160517456684	32.78889186740254	23.708562334540385	17.389385280600386
8	23.61477586052379	37.27682672212468	21.798457227681332	17.309940189670197
9	24.662066495040225	18.121920315539693	22.00297314238368	35.2130400470364
10-11	23.400857047366767	27.476714084346206	29.537199088254557	19.585229780032478
12-13	24.116815388556546	24.869475946111415	29.442766350205492	21.570942315126548
14-15	21.449115075360307	26.591688824194048	26.78620055019022	25.172995550255422
16-17	21.446796354525347	29.593233469052045	27.48621779783474	21.473752378587864
18-19	21.418771729883286	29.289950624732047	28.4628766931259	20.828400952258768
20-21	22.264540401727476	28.38987596901555	28.21630786747938	21.129275761777595
22-23	21.948545740808353	28.27810387925444	28.04759006789872	21.725760312038485
24-25	21.796723673308165	28.367725521574084	28.091018889374386	21.744531915743366
26-27	21.80567227985192	28.49450316057247	28.001397752855816	21.69842680671979
28-29	21.69485693449775	28.64791352644972	28.002003533305775	21.65522600574676
30-31	21.97542461672696	28.376138197228684	27.979483113867786	21.668954072176565
32-33	21.774612514635717	28.438648348015743	28.01201384509582	21.77472529225272
34-35	21.934426296257122	28.457103333181127	27.98626149010792	21.622208880453826
36-37	21.883967512265603	28.353022353358032	28.051616481273822	21.711393653102544
38-39	21.7102643134157	28.451738500560992	28.187449856931522	21.65054732909179
40-41	21.934673771911267	28.331189622526015	28.11075310507106	21.62338350049166
42-43	21.812438665055065	28.367044455057393	28.01338944833845	21.80712743154909
44-45	21.876333873678558	28.396003434341427	27.962079699263075	21.765582992716944
46-47	21.848181513845873	28.356505192933056	27.950479474755607	21.84483381846546
48-49	21.7487290209225	28.308126368580606	28.150831386567788	21.792313223929106
50-51	21.683609909046528	28.42658847782816	28.150475794914424	21.739325818210883
52-53	21.831005589637527	28.350835474737607	28.03419700882982	21.783961926795044
54-55	21.827372224506522	28.244418545541784	28.077595241140678	21.850613988811016
56-57	21.85166189301453	28.306624909527063	28.05033259986942	21.79138059758899
58-59	21.824332695472553	28.321520296640802	28.121936209183772	21.732210798702873
60-61	21.842679247321918	28.31357310599514	28.11698369149744	21.726763955185504
62-63	21.77880913061392	28.387916064359274	28.08406412353481	21.749210681492
64-65	21.787041701561467	28.373846956299765	28.041766092341895	21.79734524979687
66-67	21.80868709354761	28.321934579184223	28.005550526766864	21.863827800501305
68-69	21.81990518026724	28.320944753241147	28.06695425900676	21.792195807484852
70-71	21.804122883311265	28.11915710406313	28.147409564773422	21.92931044785218
72-73	21.800982896955016	28.199600429062126	28.136379703001403	21.86303697098145
74-75	21.695454570682344	28.310145831339728	28.10155832722789	21.89284127075003
76-77	21.78802142968209	28.27811923952479	27.992315456517613	21.94154387427551
78-79	21.763300244652687	28.292387879983334	28.019639635063697	21.924672240300282
80-81	21.748375197486496	28.289249951427607	28.06305043196355	21.89932441912234
82-83	21.794823886835097	28.29293733139989	28.13205628598366	21.780182495781357
84-85	21.88742807039774	28.275586289320277	28.122485836995807	21.714499803286177
86-87	21.687466944692165	28.309853856418332	28.17002970670438	21.832649492185116
88-89	21.77174213714006	28.30973102253379	28.10443535703051	21.814091483295638
90-91	21.772492186878612	28.34303951599709	28.103456526195377	21.781011770928917
92-93	21.72260309931591	28.352947155577713	28.166987055180126	21.757462689926253
94-95	21.64076293122995	28.416175733959452	28.18765351003937	21.755407824771222
96-97	21.670025521313736	28.572152286172553	28.26520412522483	21.49261806728888
98-99	22.721997254533537	30.510367770095257	28.48565857194696	18.281976403424245
100	21.739728210341543	31.541904590418635	22.21602239831786	24.502344800921954
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3234.0
1	2518.5
2	2121.0
3	2410.0
4	2438.0
5	2762.0
6	3361.5
7	3966.0
8	4336.0
9	4696.5
10	5256.0
11	5728.0
12	6195.0
13	6985.0
14	8131.0
15	9662.5
16	11641.5
17	14159.0
18	17561.0
19	22518.5
20	29545.0
21	38773.0
22	51289.5
23	68050.0
24	89135.0
25	115310.5
26	147772.5
27	184660.5
28	223606.5
29	264349.5
30	304321.0
31	343181.5
32	384523.5
33	425015.5
34	462443.0
35	496497.5
36	531485.5
37	563547.5
38	585404.0
39	602896.0
40	621532.0
41	638919.0
42	650745.0
43	660117.5
44	666393.0
45	671584.5
46	674494.0
47	675611.5
48	669777.5
49	646389.0
50	617665.5
51	586485.5
52	549751.5
53	514573.5
54	477858.5
55	437180.5
56	396576.5
57	355339.0
58	313647.5
59	271647.5
60	227351.5
61	185161.5
62	148026.5
63	115438.0
64	87576.0
65	66039.5
66	49962.0
67	38768.0
68	30740.5
69	23594.5
70	18044.5
71	13873.5
72	10756.5
73	8305.0
74	6333.0
75	4703.0
76	3481.0
77	2644.5
78	2050.0
79	1618.5
80	1245.5
81	911.0
82	684.5
83	461.5
84	314.0
85	240.0
86	193.0
87	150.0
88	97.5
89	65.0
90	49.0
91	41.5
92	39.0
93	25.0
94	12.0
95	10.5
96	9.5
97	8.0
98	9.5
99	20.0
100	58.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011863921080890145
3	0.022027517265488922
4	0.018729142654637997
5	0.011199132399633369
6	0.014075621885840571
7	0.015417983646070596
8	0.0018089922768814175
9	9.588298287357337E-5
10-11	6.072588915326313E-5
12-13	0.0031737267331152785
14-15	1.598049714559556E-4
16-17	4.506500195057948E-4
18-19	1.278439771647645E-5
20-21	6.075855197404855E-5
22-23	6.40031643164438E-6
24-25	9.608201868577478E-6
26-27	0.0025037102035904294
28-29	0.0038355182146192304
30-31	0.0027197363746029857
32-33	0.004198373299505765
34-35	0.006142695891896824
36-37	0.0011545871927545373
38-39	0.0013428888786180344
40-41	6.68471539824192E-4
42-43	6.260134828807058E-5
44-45	8.618996148170621E-5
46-47	0.004195290681077908
48-49	7.426969949168944E-4
50-51	3.58692085482821E-4
52-53	0.004255144457725447
54-55	2.0243007353323885E-4
56-57	1.6249233088057498E-4
58-59	5.574456835378067E-4
60-61	3.861852140073449E-5
62-63	3.537522179379684E-6
64-65	0.0
66-67	0.001855126619042882
68-69	0.0
70-71	3.951889261037325E-4
72-73	0.0017647146559773691
74-75	1.676206641562803E-4
76-77	2.25569218283107E-5
78-79	1.1388551408148828E-5
80-81	0.0031019415316612753
82-83	1.1229829773593466E-4
84-85	3.4806102049379377E-4
86-87	0.013465310231581209
88-89	6.231330723444316E-4
90-91	8.648838796395261E-4
92-93	3.273584148258007E-5
94-95	1.8255522969908052E-4
96-97	5.50192192713534E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	5690.0
20-21	11065.0
22-23	12361.0
24-25	13907.0
26-27	18121.0
28-29	21764.0
30-31	33998.0
32-33	41295.0
34-35	52358.0
36-37	72863.0
38-39	81701.0
40-41	82540.0
42-43	88781.0
44-45	100731.0
46-47	102994.0
48-49	102637.0
50-51	100135.0
52-53	100995.0
54-55	110020.0
56-57	111533.0
58-59	109474.0
60-61	108263.0
62-63	108482.0
64-65	114720.0
66-67	121251.0
68-69	121596.0
70-71	121688.0
72-73	119759.0
74-75	122376.0
76-77	127176.0
78-79	131298.0
80-81	128958.0
82-83	126451.0
84-85	129110.0
86-87	135527.0
88-89	142940.0
90-91	151506.0
92-93	161259.0
94-95	199632.0
96-97	534496.0
98-99	1027199.0
100-101	1.0335419E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.9737606893555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.53931246190005	73.46439723813766
2	7.617064677023828	12.640330033334363
3	2.0943664524777432	5.213323824711082
4	0.7899260874069178	2.621725525551221
5	0.3810820876172531	1.5809906970476981
6	0.20084240701905443	0.9998789889763894
7	0.11097941519813966	0.6445865606666544
8	0.07412939688189298	0.4920635869539555
9	0.04802264855444534	0.35861577739430145
>10	0.1415319357685309	1.7347427229902628
>50	0.0022796891454581504	0.12611720111937783
>100	3.6913535905218944E-4	0.049368757675594316
>500	6.688400475951481E-5	0.035634644049469505
>1k	2.672164283200344E-5	0.03822444139203087
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.392198858238224E-6	0.0
2	0.0	1.917659657471467E-5	0.0	1.2784397716476448E-5	0.0
3	0.0	1.917659657471467E-5	0.0	2.5568795432952896E-5	0.0
4	0.0	4.474539200766757E-5	0.0	2.5568795432952896E-5	0.0
5	0.0	5.113759086590579E-5	0.0	3.196099429119112E-5	0.0
6	0.0	5.113759086590579E-5	0.0	3.196099429119112E-5	0.0
7	0.0	5.113759086590579E-5	0.0	3.196099429119112E-5	0.0
8	0.0	6.392198858238224E-5	0.0	3.196099429119112E-5	6.392198858238224E-6
9	0.0	1.7898156803067028E-4	0.0	5.113759086590579E-5	6.392198858238224E-6
10-11	0.0	1.7898156803067028E-4	0.0	7.031418744062046E-5	6.392198858238224E-6
12-13	0.0	1.853737668889085E-4	0.0	1.1825567887740714E-4	6.392198858238224E-6
14-15	0.0	2.4929575547129074E-4	0.0	2.620801531877672E-4	1.598049714559556E-5
16-17	3.196099429119112E-6	2.4929575547129074E-4	0.0	3.8672803092341257E-4	2.5568795432952896E-5
18-19	6.392198858238224E-6	2.5888405375864806E-4	0.0	5.145720080881771E-4	3.196099429119112E-5
20-21	6.392198858238224E-6	2.812567497624819E-4	0.0	7.031418744062047E-4	3.196099429119112E-5
22-23	6.392198858238224E-6	3.260021417701494E-4	0.0	0.0010067713201725203	5.113759086590579E-5
24-25	1.2784397716476448E-5	3.611592354904597E-4	0.0	0.0014158720470997667	7.031418744062047E-5
26-27	1.2784397716476448E-5	3.70747533777817E-4	0.0	0.001940032353475301	7.031418744062047E-5
28-29	1.2784397716476448E-5	3.70747533777817E-4	0.0	0.00464393247051007	7.031418744062047E-5
30-31	1.598049714559556E-5	4.186890252146037E-4	0.0	0.013311754122281102	7.031418744062047E-5
32-33	2.5568795432952896E-5	4.28277323501961E-4	0.0	0.033562240105179796	7.670638629885869E-5
34-35	2.5568795432952896E-5	4.921993120843432E-4	0.0	0.05314793740682172	7.670638629885869E-5
36-37	2.5568795432952896E-5	5.561213006667255E-4	0.0	0.07855692786831867	8.309858515709691E-5
38-39	2.5568795432952896E-5	5.657095989540829E-4	0.0	0.10247973209527521	8.309858515709691E-5
40-41	2.5568795432952896E-5	6.488081841111797E-4	0.0	0.1216083871785531	8.309858515709691E-5
42-43	2.5568795432952896E-5	6.679847806858944E-4	0.0	0.14172783308485792	8.629468458621603E-5
44-45	2.5568795432952896E-5	7.734560618468251E-4	0.0	0.1576795653355914	8.949078401533514E-5
46-47	2.5568795432952896E-5	8.629468458621603E-4	0.0	0.1705918070292326	9.268688344445426E-5
48-49	2.5568795432952896E-5	8.661429452912794E-4	0.0	0.1792340598855707	1.0866738059004981E-4
50-51	2.5568795432952896E-5	9.97183021885163E-4	0.0	0.18589792719528403	1.0866738059004981E-4
52-53	2.5568795432952896E-5	0.0010035752207434012	0.0	0.19163492567055285	1.2784397716476448E-4
54-55	2.5568795432952896E-5	0.0010099674196016395	0.0	0.1961446219650399	1.5021667316859828E-4
56-57	2.5568795432952896E-5	0.0010163596184598776	0.0	0.20012056965486408	1.5341277259771737E-4
58-59	2.5568795432952896E-5	0.0010259479167472351	0.0	0.2033326495811288	1.5660887202683648E-4
60-61	2.5568795432952896E-5	0.0010355362150345922	0.0	0.20657029830282647	1.598049714559556E-4
62-63	2.5568795432952896E-5	0.0010515167121801878	0.0	0.20956504346791105	1.7258936917243206E-4
64-65	2.5568795432952896E-5	0.0011569879933411185	0.0	0.21215068790606845	1.7258936917243206E-4
66-67	2.5568795432952896E-5	0.001166576291628476	0.0	0.2146532337590687	1.7898156803067028E-4
68-69	2.5568795432952896E-5	0.0012017333853487863	0.0	0.21693844485088887	1.853737668889085E-4
70-71	3.196099429119112E-5	0.001220909981923501	0.0	0.21879857471863617	1.9496206517626583E-4
72-73	3.835319314942934E-5	0.001230498280210858	0.0	0.22027836875431833	2.109425623218614E-4
74-75	4.474539200766757E-5	0.0012592631750729302	0.0	0.22162392661397748	2.109425623218614E-4
76-77	5.752978972414402E-5	0.0013008124676514785	0.0	0.22303979866107726	2.109425623218614E-4
78-79	5.752978972414402E-5	0.0013295773625135506	0.0	0.22407853097554095	2.109425623218614E-4
80-81	6.392198858238224E-5	0.0013295773625135506	0.0	0.22458031858591265	2.141386617509805E-4
82-83	6.392198858238224E-5	0.0013391656608009081	0.0	0.2249254973242575	2.1733476118009963E-4
84-85	6.392198858238224E-5	0.0013583422573756228	0.0	0.2250565374008514	2.2053086060921874E-4
86-87	6.392198858238224E-5	0.001425460345387124	0.0	0.22510447889228818	2.3011915889657608E-4
88	6.392198858238224E-5	0.001508558930544221	0.0	0.2251076749917173	2.365113577548143E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	23570	0.0	20.36167	1
GTACATG	38500	0.0	19.907042	1
GGTATCA	17855	0.0	19.840979	1
TCAACGC	22565	0.0	19.61345	4
TACATGG	37650	0.0	19.513506	2
ATCAACG	22750	0.0	19.51169	3
CAACGCA	23160	0.0	19.089579	5
AACGCAG	24050	0.0	18.452168	6
ACATGGG	38890	0.0	18.372702	3
TATCAAC	26990	0.0	17.082384	2
CATGGGG	28010	0.0	16.367193	4
CGCAGAG	27545	0.0	16.046783	8
ACGCAGA	27755	0.0	15.95759	7
AGAGTAC	24780	0.0	13.978883	10-11
TAGCCCT	10135	0.0	13.413591	4
TATACTG	9640	0.0	13.389542	5
GTATAGA	7980	0.0	13.167584	1
GTGTAGC	9635	0.0	13.086953	1
GAGTACA	27950	0.0	12.797559	1
GTATAGG	5170	0.0	12.775358	1
>>END_MODULE