FastQCFastQC Report
Fri 27 May 2016
SRR2138829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7831117
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79050.040.5514031
GTATCAA134050.032.8924641
TCAACGC152650.028.0989954
ATCAACG155150.027.6727073
CAACGCA155750.027.6186855
AACGCAG162400.026.4619436
TATCAAC167650.025.8808162
GTACATG224150.023.6198561
TACATGG229750.022.4500332
ACGCAGA191750.022.3252937
CGCAGAG194700.022.0082748
ACATGGG229750.021.7299043
CATGGGG166350.019.8670254
GCAGAGT242350.017.595759
GAGTACT119100.017.22555412-13
AGAGTAC206550.016.39020210-11
ATGGGGG108300.016.15515
CAGAGTA230900.015.99625510-11
GAGTACA164300.015.4825911
GTACTTT136150.014.99307814-15
Produced by FastQC (version 0.11.5)