##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138829_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7831117
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50893927392478	34.0	31.0	34.0	31.0	34.0
2	32.70273780866765	34.0	31.0	34.0	31.0	34.0
3	32.80796749684624	34.0	31.0	34.0	31.0	34.0
4	36.25487092071285	37.0	37.0	37.0	35.0	37.0
5	36.10960313835178	37.0	35.0	37.0	35.0	37.0
6	36.15318287289029	37.0	36.0	37.0	35.0	37.0
7	36.14346510210485	37.0	36.0	37.0	35.0	37.0
8	36.109036041729425	37.0	36.0	37.0	35.0	37.0
9	37.978159948318996	39.0	38.0	39.0	35.0	39.0
10-11	37.865370610603826	39.0	38.0	39.0	35.0	39.0
12-13	37.888574069318594	39.0	38.0	39.0	35.0	39.0
14-15	39.266497103797576	40.0	39.0	41.0	36.0	41.0
16-17	39.243602157904164	40.0	39.0	41.0	36.0	41.0
18-19	39.273531732446344	40.0	39.0	41.0	36.0	41.0
20-21	39.272500387966986	40.0	39.0	41.0	36.0	41.0
22-23	39.237919137562166	40.0	39.0	41.0	36.0	41.0
24-25	39.24332456079115	40.0	39.0	41.0	36.0	41.0
26-27	39.12399673137678	40.0	39.0	41.0	36.0	41.0
28-29	39.0419338110258	40.0	39.0	41.0	36.0	41.0
30-31	38.95604665668512	40.0	38.5	41.0	35.0	41.0
32-33	38.88080082424331	40.0	38.0	41.0	35.0	41.0
34-35	38.82377622273469	40.0	38.0	41.0	35.0	41.0
36-37	38.79158504790818	40.0	38.0	41.0	35.0	41.0
38-39	38.73140696631937	40.0	38.0	41.0	35.0	41.0
40-41	38.70555672088033	40.0	38.0	41.0	35.0	41.0
42-43	38.677711122168475	40.0	38.0	41.0	35.0	41.0
44-45	38.61424937706418	40.0	38.0	41.0	35.0	41.0
46-47	38.53564148629425	40.0	38.0	41.0	34.0	41.0
48-49	38.500473594286554	40.0	38.0	41.0	34.0	41.0
50-51	38.42880555395173	40.0	38.0	41.0	34.0	41.0
52-53	38.25994530399505	40.0	37.5	41.0	34.0	41.0
54-55	38.11548684892691	40.0	37.0	41.0	33.5	41.0
56-57	37.96676842282157	40.0	37.0	41.0	33.0	41.0
58-59	37.799232473919744	39.0	36.5	41.0	33.0	41.0
60-61	37.66265535203841	39.0	36.0	41.0	33.0	41.0
62-63	37.45396446870157	39.0	35.5	40.5	33.0	41.0
64-65	37.21845602154198	39.0	35.0	40.0	33.0	41.0
66-67	36.97420053805214	38.0	35.0	40.0	32.0	41.0
68-69	36.69168394796341	37.5	35.0	40.0	32.0	41.0
70-71	36.38421355899045	37.0	35.0	39.5	32.0	41.0
72-73	36.066067998297655	36.5	35.0	39.0	32.0	41.0
74-75	35.67021606513168	36.0	34.5	39.0	31.0	40.0
76-77	33.710258917309304	34.5	32.0	36.5	29.0	39.0
78-79	34.822514811304984	35.0	34.0	37.0	30.5	39.0
80-81	34.79402106762082	35.0	34.0	37.0	31.0	39.0
82-83	34.53367042926894	35.0	34.0	36.0	31.0	38.5
84-85	34.234194211859496	35.0	34.0	36.0	31.0	37.0
86-87	34.065528661443366	35.0	34.0	36.0	31.0	37.0
88-89	33.8869133563759	35.0	34.0	35.0	30.5	36.5
90-91	33.73179056175391	35.0	34.0	35.0	30.0	36.0
92-93	33.66149553079383	35.0	34.0	35.0	30.0	36.0
94-95	33.60011972047053	35.0	34.0	35.0	30.0	36.0
96-97	33.62423009019672	35.0	34.0	35.0	30.5	35.5
98-99	33.66164833735667	35.0	34.0	35.0	31.0	35.0
100-101	32.40381399562699	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	5.0
20	26.0
21	119.0
22	503.0
23	1566.0
24	4306.0
25	9512.0
26	18144.0
27	30416.0
28	45743.0
29	64769.0
30	87832.0
31	117402.0
32	156312.0
33	217735.0
34	325436.0
35	514644.0
36	961381.0
37	1879786.0
38	2345111.0
39	1031059.0
40	19306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.34129052087972	19.921117255686514	13.260471015820604	24.477121207613166
2	14.881522615530743	21.224217355093497	40.937506145489955	22.956753883885803
3	18.5693049918677	25.939632877404335	29.990945097615068	25.5001170331129
4	12.54351378368535	17.590027240969437	38.83818772210227	31.028271253242938
5	13.262357253250473	37.85362418943266	35.65426213391267	13.229756423404204
6	30.285131189155233	37.73300215793521	18.844562900957555	13.137303751951993
7	26.83536716409677	32.197526866218446	23.31695465666009	17.650151313024693
8	24.615058107291716	36.71394514984261	21.32741727648814	17.34357946637753
9	25.82789913622795	16.41850070685957	21.09598158219319	36.657618574719294
10-11	24.460955990012113	26.56222904460505	28.973190946951675	20.00362401843116
12-13	25.093880771053634	24.074078448856937	29.086792856639825	21.745247923449604
14-15	22.175518928305934	25.429965884170652	26.501894908841532	25.892620278681882
16-17	22.01028920906175	28.540513441441366	27.130114388534864	22.31908296096202
18-19	22.031109313703393	28.00775530067497	28.491401706876147	21.46973367874549
20-21	22.81678163918315	27.186963196343868	28.30518449332886	21.691070671144125
22-23	22.562897218366718	27.140917917016388	28.127447294253592	22.1687375703633
24-25	22.294469606907562	27.230107700224675	28.23443817882684	22.240984514040925
26-27	22.28944138048953	27.390779357164963	28.135904397450457	22.18387486489505
28-29	22.178693036610508	27.49852843631381	28.141674977713947	22.18110354936174
30-31	22.510600256114945	27.308368210645	28.060528131717426	22.120503401522626
32-33	22.212731139788115	27.50524042470331	28.06133618091508	22.220692254593498
34-35	22.372887915978186	27.513176385362947	28.055455479928128	22.05848021873074
36-37	22.359383920101582	27.481966208631736	27.971680292399775	22.186969578866908
38-39	22.225027248095515	27.555777106782426	28.061918826202053	22.15727681892001
40-41	22.43656064327038	27.422584177030217	27.952436790643283	22.188418389056125
42-43	22.294387908552057	27.517023551595905	27.8297439972549	22.358844542597133
44-45	22.407088162088996	27.57187972695429	27.695326580614584	22.325705530342134
46-47	22.43784969929626	27.580577323579273	27.51426053467262	22.467312442451846
48-49	22.353592505219886	27.635950434085593	27.53114468159564	22.479312379098882
50-51	22.317627714225825	27.71777405225828	27.478986215461234	22.485612018054656
52-53	22.54795140757028	27.6141811634667	27.354226992109805	22.483640436853214
54-55	22.479930971313728	27.537746216827724	27.420671069136805	22.561651742721747
56-57	22.48084118361388	27.64754554861797	27.39106894046047	22.48054432730768
58-59	22.448067955006973	27.68585103153794	27.361592552170645	22.504488461284442
60-61	22.551083526072183	27.633295594569596	27.384448353732406	22.43117252562582
62-63	22.48688544910247	27.662826643028048	27.368751197268537	22.481536710600945
64-65	22.496436822292264	27.677589438173538	27.28705163299676	22.538922106537438
66-67	22.564437017915353	27.633807352863904	27.182778809444365	22.618976819776382
68-69	22.45913080729458	27.67210612318453	27.261909846924283	22.606853222596605
70-71	22.45101689020455	27.530898338784045	27.33598250149311	22.682102269518303
72-73	22.53686653719752	27.510428006820657	27.278348637773085	22.674356818208736
74-75	22.390429103801807	27.64348860602386	27.263080279098467	22.703002011075867
76-77	22.53857048865145	27.58135490496584	27.20631205981118	22.673762546571535
78-79	22.474667633530782	27.52932400730803	27.195331776039012	22.80067658312218
80-81	22.50635952944541	27.601066093909893	27.18564766689085	22.70692670975384
82-83	22.530483936011525	27.58765852518942	27.25548119109765	22.626376347701406
84-85	22.674333297366207	27.513558088854396	27.182270548169296	22.6298380656101
86-87	22.46279371980583	27.573677772259426	27.31106897486471	22.652459533070033
88-89	22.547251352537074	27.524081616430294	27.247616790860324	22.6810502401723
90-91	22.591492294132948	27.543689146403782	27.23477928118156	22.63003927828171
92-93	22.58574711019653	27.54726898244644	27.252656803395308	22.614327103961728
94-95	22.435290533976588	27.56101408100733	27.35672009975107	22.64697528526501
96-97	22.489294441350786	27.58472447887723	27.31839944386609	22.60758163590589
98-99	22.773015085089966	28.11820223133519	27.403087775159168	21.70569490841568
100-101	26.99834267819178	35.1005194972128	28.012984462059563	9.888153362535855
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1149.0
1	1070.5
2	1504.5
3	2153.0
4	2939.5
5	4935.5
6	6636.5
7	7631.5
8	8171.0
9	7952.0
10	7348.0
11	6609.5
12	6927.5
13	9052.0
14	13990.0
15	20397.5
16	25716.0
17	29137.5
18	30331.5
19	29808.0
20	28747.5
21	28092.5
22	29041.0
23	33020.0
24	40460.5
25	50849.5
26	64049.0
27	78965.5
28	95563.0
29	114428.5
30	132939.5
31	151297.0
32	170339.0
33	189028.0
34	206574.5
35	223173.0
36	242190.5
37	259798.5
38	274975.0
39	288488.0
40	300490.0
41	311045.0
42	317761.5
43	325054.0
44	332607.5
45	339789.0
46	345466.0
47	348800.0
48	349987.0
49	346203.0
50	340188.5
51	331624.5
52	318331.5
53	303422.5
54	288151.0
55	269742.0
56	248567.0
57	225888.0
58	201627.5
59	175524.0
60	148011.5
61	122476.5
62	99269.0
63	78519.5
64	60292.0
65	45586.0
66	34883.5
67	27038.0
68	20859.5
69	15128.5
70	10632.5
71	7503.5
72	5205.0
73	3539.5
74	2381.0
75	1620.0
76	1107.5
77	749.5
78	502.0
79	332.0
80	215.0
81	143.5
82	106.0
83	77.0
84	55.0
85	41.0
86	32.0
87	21.0
88	12.5
89	8.0
90	5.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0021835965418470953
3	0.0
4	0.00338393616134199
5	3.5754797176443666E-4
6	2.553914084031691E-4
7	0.0
8	0.0
9	0.0
10-11	3.95856683024912E-4
12-13	5.171676020164173E-4
14-15	1.9154355630237678E-5
16-17	0.0
18-19	0.0010981830561336268
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.11155848606883E-5
28-29	0.0
30-31	7.045269636239278E-5
32-33	0.00101520853057882
34-35	7.998818755088361E-4
36-37	3.6333137761568584E-4
38-39	0.0017541103780500575
40-41	1.3218389741049102E-5
42-43	6.6732197684793135E-6
44-45	1.2143625901841318E-4
46-47	6.83114795801959E-6
48-49	0.0
50-51	1.609645781349356E-4
52-53	2.122940924215255E-5
54-55	0.003463049087361352
56-57	0.002990194839792528
58-59	0.0016052868031790836
60-61	1.261750702850807E-4
62-63	1.5024544095233975E-5
64-65	1.3680394816194394E-4
66-67	3.846789454134421E-5
68-69	1.6368539697878847E-4
70-71	0.0
72-73	0.0
74-75	1.539685806852088E-4
76-77	1.641576537274868E-5
78-79	4.2411004042267633E-4
80-81	0.0
82-83	1.9667568495941514E-4
84-85	3.466709492084592E-5
86-87	0.0015986766470937113
88-89	0.0
90-91	1.7196324005172293E-4
92-93	1.83971602879266E-5
94-95	1.496862576040619E-4
96-97	2.8615553755319275E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	238.0
20-21	575.0
22-23	1176.0
24-25	2833.0
26-27	4517.0
28-29	9580.0
30-31	24002.0
32-33	28578.0
34-35	38908.0
36-37	62585.0
38-39	74989.0
40-41	71963.0
42-43	78213.0
44-45	90324.0
46-47	91172.0
48-49	86681.0
50-51	80193.0
52-53	76594.0
54-55	81007.0
56-57	84653.0
58-59	84580.0
60-61	82470.0
62-63	77294.0
64-65	78330.0
66-67	83770.0
68-69	84916.0
70-71	83211.0
72-73	78284.0
74-75	78665.0
76-77	78151.0
78-79	81936.0
80-81	83636.0
82-83	78692.0
84-85	76629.0
86-87	80254.0
88-89	85339.0
90-91	88331.0
92-93	90201.0
94-95	96639.0
96-97	147195.0
98-99	627405.0
100-101	4496408.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.93318132437732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.81969314177589	78.9524485171831
2	6.591634500351034	11.460635144860754
3	1.5085418803557409	3.9342703446114826
4	0.5113933794644567	1.7782821354027885
5	0.23057218923016504	1.0022186967352287
6	0.11741341156926498	0.6124272838718792
7	0.06820881343962755	0.4150726402667436
8	0.03839045867507477	0.2669923764101031
9	0.025807026109480376	0.2019138192196558
>10	0.0857333436680573	1.1844254167288764
>50	0.0022036675247560557	0.1310325044996411
>100	3.8576038838927297E-4	0.0499282694890918
>500	2.2427447972694934E-5	0.010352850720655166
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.5539140840316905E-5	0.0
4	0.0	5.107828168063381E-5	0.0	2.5539140840316905E-5	0.0
5	0.0	6.384785210079226E-5	0.0	2.5539140840316905E-5	0.0
6	0.0	6.384785210079226E-5	0.0	3.8308711260475355E-5	1.2769570420158452E-5
7	0.0	6.384785210079226E-5	0.0	5.107828168063381E-5	2.5539140840316905E-5
8	0.0	6.384785210079226E-5	0.0	6.384785210079226E-5	2.5539140840316905E-5
9	0.0	1.915435563023768E-4	0.0	1.1492613378142607E-4	2.5539140840316905E-5
10-11	0.0	1.915435563023768E-4	0.0	1.5323484504190145E-4	2.5539140840316905E-5
12-13	0.0	1.915435563023768E-4	0.0	1.8515877109229756E-4	3.192392605039613E-5
14-15	0.0	2.2985226756285214E-4	0.0	2.1069791193261447E-4	4.469349647055458E-5
16-17	0.0	2.617761936132483E-4	0.0	2.3623705277293135E-4	5.107828168063381E-5
18-19	0.0	2.873153344535652E-4	0.0	2.8731533445356517E-4	5.107828168063381E-5
20-21	0.0	2.9370011966364443E-4	0.0	3.7670232739467433E-4	5.107828168063381E-5
22-23	0.0	3.0646969008380284E-4	0.0	4.597045351257043E-4	5.107828168063381E-5
24-25	0.0	3.2562404571404057E-4	0.0	5.299371724365757E-4	5.107828168063381E-5
26-27	0.0	3.320088309241198E-4	0.0	6.384785210079226E-4	5.107828168063381E-5
28-29	0.0	3.4477840134427824E-4	0.0	0.0011109526265537853	5.107828168063381E-5
30-31	0.0	3.830871126047536E-4	0.0	0.0030583121156279496	5.7463066890713036E-5
32-33	0.0	3.95856683024912E-4	0.0	0.006965800664196435	6.384785210079226E-5
34-35	0.0	4.660893203357835E-4	0.0	0.012169400610411005	7.661742252095071E-5
36-37	0.0	5.10782816806338E-4	0.0	0.01947359489074164	7.661742252095071E-5
38-39	0.0	5.235523872264966E-4	0.0	0.030895975631573376	7.661742252095071E-5
40-41	0.0	5.874002393272887E-4	0.0	0.042452436861816774	7.661742252095071E-5
42-43	0.0	6.257089505877641E-4	0.0	0.052355238722649654	7.661742252095071E-5
44-45	0.0	6.959415878986356E-4	0.0	0.06271136033339816	8.300220773102993E-5
46-47	0.0	7.470198695792695E-4	0.0	0.07417204978549037	8.938699294110916E-5
48-49	0.0	7.597894399994279E-4	0.0	0.08496872157573435	1.0215656336126762E-4
50-51	0.0	8.619460033606956E-4	0.0	0.09575900858076823	1.0215656336126762E-4
52-53	0.0	8.683307885707748E-4	0.0	0.1054511125296685	1.0215656336126762E-4
54-55	0.0	8.747155737808541E-4	0.0	0.11491336421100593	1.0854134857134684E-4
56-57	0.0	8.811003589909332E-4	0.0	0.12431176804024253	1.1492613378142607E-4
58-59	0.0	8.938699294110916E-4	0.0	0.1336016305209078	1.2131091899150529E-4
60-61	0.0	8.938699294110916E-4	0.0	0.14232324711787603	1.276957042015845E-4
62-63	0.0	9.130242850413294E-4	0.0	0.15090439844022252	1.276957042015845E-4
64-65	0.0	0.001021565633612676	0.0	0.15915992571685494	1.276957042015845E-4
66-67	0.0	0.0010598743448731514	0.0	0.16752399434205872	1.3408048941166375E-4
68-69	0.0	0.0010662591300832306	0.0	0.1754028192912965	1.468500598318222E-4
70-71	0.0	0.0010790287005033893	0.0	0.18273893749767756	1.5961963025198065E-4
72-73	0.0	0.0010854134857134685	0.0	0.1884022419790178	1.7877398588221833E-4
74-75	0.0	0.0011492613378142607	0.0	0.19306313518237564	1.915435563023768E-4
76-77	0.0	0.0012258787603352116	0.0	0.19745586740691015	1.915435563023768E-4
78-79	0.0	0.0012386483307553699	0.0	0.20105050148018477	1.915435563023768E-4
80-81	0.0	0.0012386483307553699	0.0	0.20272331520522552	1.915435563023768E-4
82-83	0.0	0.0012386483307553699	0.0	0.20335540894102336	1.915435563023768E-4
84-85	0.0	0.0012578026863856076	0.0	0.2039938874620313	1.915435563023768E-4
86-87	0.0	0.0012769570420158452	0.0	0.2042365093000143	1.915435563023768E-4
88-89	0.0	0.001359959249746875	0.0	0.20431312672253524	1.915435563023768E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7905	0.0	40.551403	1
GTATCAA	13405	0.0	32.892464	1
TCAACGC	15265	0.0	28.098995	4
ATCAACG	15515	0.0	27.672707	3
CAACGCA	15575	0.0	27.618685	5
AACGCAG	16240	0.0	26.461943	6
TATCAAC	16765	0.0	25.880816	2
GTACATG	22415	0.0	23.619856	1
TACATGG	22975	0.0	22.450033	2
ACGCAGA	19175	0.0	22.325293	7
CGCAGAG	19470	0.0	22.008274	8
ACATGGG	22975	0.0	21.729904	3
CATGGGG	16635	0.0	19.867025	4
GCAGAGT	24235	0.0	17.59575	9
GAGTACT	11910	0.0	17.225554	12-13
AGAGTAC	20655	0.0	16.390202	10-11
ATGGGGG	10830	0.0	16.1551	5
CAGAGTA	23090	0.0	15.996255	10-11
GAGTACA	16430	0.0	15.482591	1
GTACTTT	13615	0.0	14.993078	14-15
>>END_MODULE