FastQCFastQC Report
Fri 27 May 2016
SRR2138828_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138828_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25916825
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA419200.029.097771
GTATCAA633550.024.9161341
TCAACGC602250.024.7596724
ATCAACG604950.024.6491223
CAACGCA609800.024.425085
AACGCAG628200.023.7760946
GTACATG682300.021.0827671
TATCAAC741100.021.0068442
TACATGG681300.020.5797352
ACGCAGA730650.020.3849987
CGCAGAG738150.020.1438878
ACATGGG680200.019.998023
CATGGGG495850.017.8698564
AGAGTAC718600.016.79616410-11
GAGTACT460800.016.71967712-13
GCAGAGT888350.016.672119
CAGAGTA776750.016.55618110-11
GTACTTT520100.014.86669214-15
AGTACTT502600.014.60532512-13
GAGTACA501100.014.52843951
Produced by FastQC (version 0.11.5)