##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138828_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25916825
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.213757896655935	33.0	31.0	34.0	30.0	34.0
2	32.43871585350443	34.0	31.0	34.0	31.0	34.0
3	32.53442028489215	34.0	31.0	34.0	31.0	34.0
4	36.072743941435725	37.0	35.0	37.0	35.0	37.0
5	35.87614933542206	37.0	35.0	37.0	35.0	37.0
6	35.90906432404432	37.0	35.0	37.0	35.0	37.0
7	35.89267500938097	37.0	35.0	37.0	35.0	37.0
8	35.847988324187085	37.0	35.0	37.0	35.0	37.0
9	37.66430425023127	39.0	37.0	39.0	35.0	39.0
10-11	37.52339036513925	39.0	37.0	39.0	35.0	39.0
12-13	37.54362696819537	39.0	37.0	39.0	35.0	39.0
14-15	38.821972888268526	40.0	38.0	41.0	35.5	41.0
16-17	38.78205819964444	40.0	38.0	41.0	35.0	41.0
18-19	38.81183086662814	40.0	38.0	41.0	35.0	41.0
20-21	38.81100839339169	40.0	38.0	41.0	35.0	41.0
22-23	38.7729569698271	40.0	38.0	41.0	35.0	41.0
24-25	38.76965883158549	40.0	38.0	41.0	35.0	41.0
26-27	38.6309401798326	40.0	38.0	41.0	35.0	41.0
28-29	38.53692589299537	40.0	38.0	41.0	34.0	41.0
30-31	38.430420234123616	40.0	38.0	41.0	34.0	41.0
32-33	38.341308637550654	40.0	38.0	41.0	34.0	41.0
34-35	38.27130306900938	40.0	38.0	41.0	34.0	41.0
36-37	38.23622612909911	40.0	38.0	41.0	34.0	41.0
38-39	38.164969530212936	40.0	38.0	41.0	34.0	41.0
40-41	38.13541435901383	40.0	38.0	41.0	34.0	41.0
42-43	38.10115455431221	40.0	38.0	41.0	33.0	41.0
44-45	38.030893877025704	40.0	37.5	41.0	33.0	41.0
46-47	37.94827766240296	40.0	37.0	41.0	33.0	41.0
48-49	37.91252809979093	40.0	37.0	41.0	33.0	41.0
50-51	37.83612790738186	40.0	37.0	41.0	33.0	41.0
52-53	37.655118237268454	39.0	37.0	41.0	33.0	41.0
54-55	37.50529559407842	39.0	36.0	40.5	32.5	41.0
56-57	37.355623145456946	39.0	36.0	40.0	32.0	41.0
58-59	37.18842184851849	39.0	35.5	40.0	32.0	41.0
60-61	37.05899342718884	39.0	35.0	40.0	31.5	41.0
62-63	36.8574828609558	38.5	35.0	40.0	31.0	41.0
64-65	36.6384443343529	38.0	35.0	40.0	31.0	41.0
66-67	36.416912698666096	38.0	35.0	40.0	31.0	41.0
68-69	36.14944346387341	37.0	34.5	40.0	31.0	41.0
70-71	35.854230022270244	37.0	34.0	39.0	31.0	41.0
72-73	35.55110582671678	36.0	34.0	39.0	30.0	40.5
74-75	35.160411211979586	36.0	34.0	39.0	30.0	40.0
76-77	33.13606013622949	34.5	31.0	36.5	27.0	39.0
78-79	34.33184049650073	35.0	33.5	37.0	29.5	39.0
80-81	34.34667645087503	35.0	34.0	37.0	30.0	39.0
82-83	34.0844691845629	35.0	33.5	36.0	29.5	38.5
84-85	33.777963099328005	35.0	33.0	36.0	29.0	37.0
86-87	33.59933705415749	35.0	33.0	35.5	29.0	37.0
88-89	33.41729237011238	35.0	33.0	35.0	29.0	36.5
90-91	33.25135226970678	35.0	33.0	35.0	29.0	36.0
92-93	33.18186139749304	35.0	33.0	35.0	29.0	36.0
94-95	33.12587215829136	35.0	33.0	35.0	29.0	36.0
96-97	33.175291608561395	34.0	33.0	35.0	29.0	35.0
98-99	33.250716553411976	34.0	33.0	35.0	29.0	35.0
100-101	32.01766659107224	33.0	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	7.0
19	45.0
20	145.0
21	668.0
22	2874.0
23	9320.0
24	24154.0
25	51736.0
26	93781.0
27	151985.0
28	224467.0
29	311596.0
30	414465.0
31	543945.0
32	719490.0
33	983709.0
34	1435901.0
35	2211206.0
36	3817839.0
37	6290925.0
38	6397645.0
39	2205302.0
40	25617.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.750272265217674	20.58506394977008	13.616243502049343	24.0484202829629
2	14.87765753416359	21.58792876506378	40.89967519986165	22.63473850091098
3	18.378481932103952	26.307844421529257	30.527327325009914	24.786346321356877
4	12.485415077658212	18.00440486937979	39.234800281629504	30.275379771332496
5	12.866524658076234	38.15884342252588	36.05807782475127	12.916554094646617
6	29.868224116236885	38.44782887347285	18.803375271494513	12.880571738795751
7	26.2936412928667	32.65687830202967	23.596933652173828	17.452546752929806
8	24.264623463715175	37.079904656531035	21.449417511597197	17.206054368156593
9	25.45231524309016	16.55160306094593	21.44705611123276	36.549025584731154
10-11	24.09931482357041	26.854810201840273	29.284759429088258	19.761115545501056
12-13	24.752132298023994	24.341838911310404	29.45443263153291	21.45159615913269
14-15	21.783724100123077	25.859424430973828	26.874593266103382	25.482258202799713
16-17	21.555580207066257	28.970477672322904	27.56980069896679	21.904141421644045
18-19	21.52796830644007	28.458188227422248	28.963558182310358	21.05028528382733
20-21	22.340890851705606	27.63693619843656	28.741281074013546	21.280891875844294
22-23	22.016327299857576	27.53960734300655	28.64381872374001	21.80024663339587
24-25	21.81996824440408	27.632231028378758	28.739725436653924	21.808075290563238
26-27	21.855737451900296	27.800330874888036	28.6050699259307	21.738861747280968
28-29	21.77986764987992	27.93562516381616	28.615846090330386	21.668661095973533
30-31	22.029401599239172	27.856184954298396	28.480463815708422	21.63394963075401
32-33	21.766463454204075	27.955878914600774	28.474295031085795	21.80336260010936
34-35	21.890089298326558	27.965806265701847	28.482548859722677	21.66155557624891
36-37	21.940968595567114	27.89231550396626	28.431768058550805	21.734947841915826
38-39	21.81878275464293	27.967616225707292	28.499903131694186	21.713697887955593
40-41	22.009565624009465	27.831414497303996	28.42836025257423	21.730659626112303
42-43	21.888836861793912	27.995640486867046	28.238651776577516	21.876870874761526
44-45	22.025367865148887	28.07575323805543	28.025685136376875	21.873193760418804
46-47	21.97797296978969	28.028691093686714	27.905451927789443	22.087884008734157
48-49	21.988167755567968	28.099394392388277	27.912683071124427	21.999754780919325
50-51	21.93062751136287	28.211086431928074	27.84982073532918	22.008465321379877
52-53	22.089503866833915	28.080352427397102	27.707285717804986	22.122857987964
54-55	22.053224531593703	28.012953276144813	27.772776474950277	22.161045717311204
56-57	22.079872167571775	28.06717568981399	27.761846114522097	22.091106028092135
58-59	22.02296353102216	28.08088664787047	27.775862818162295	22.120287002945073
60-61	22.13005190860418	28.04467715376575	27.78317147764817	22.042099459981902
62-63	22.038115434033234	28.117100918751476	27.754612762894194	22.0901708843211
64-65	21.97912269843879	28.12567763679288	27.723889848395377	22.171309816372954
66-67	22.02317076752639	28.215883000122847	27.5561941739945	22.204752058356263
68-69	22.008954755260017	28.191952920205033	27.614872784560124	22.184219539974826
70-71	22.021133182383252	27.959653922108764	27.634658799658872	22.38455409584911
72-73	22.03363326084908	27.99770572809831	27.621760675367142	22.346900335685472
74-75	21.97348843225659	28.079286522122977	27.600312290175705	22.346912755444734
76-77	22.091069762852364	28.01706370342547	27.51862111550102	22.373245418221146
78-79	22.03847656840351	28.01703419341239	27.4999003917206	22.444588846463503
80-81	22.04366364726827	28.063115728128533	27.49055720305915	22.40266342154404
82-83	22.089913477209226	28.032009472329783	27.561480470256576	22.316596580204415
84-85	22.256089062572805	27.999576201336996	27.452862628011005	22.291472108079194
86-87	22.058859913867664	28.03403974697764	27.57632329175338	22.330777047401313
88-89	22.142602150221123	27.994386949916578	27.561060073237254	22.301950826625045
90-91	22.133854297587128	28.00242006243318	27.61261210270461	22.25111353727508
92-93	22.04375722990974	27.934059520053257	27.682188279728848	22.339994970308155
94-95	21.929496326955782	28.026768039014016	27.764806433269023	22.27892920076118
96-97	21.916315293817	28.079222213307954	27.76770446529576	22.236758027579285
98-99	22.17995869168523	28.675418322252483	27.80353864784718	21.341084338215108
100-101	26.22535156658265	35.73839091488359	28.40872669013172	9.627530828402044
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3661.0
1	3436.5
2	5310.0
3	7946.5
4	11052.0
5	18441.5
6	24670.5
7	28353.5
8	30049.5
9	28761.0
10	25903.0
11	23081.0
12	24739.0
13	32708.0
14	51564.0
15	77312.0
16	98419.5
17	111777.0
18	117177.5
19	115832.0
20	111998.5
21	109502.0
22	112749.5
23	126699.5
24	152419.5
25	190448.0
26	241095.0
27	298007.5
28	361215.0
29	429389.0
30	491676.5
31	552740.0
32	615668.0
33	673900.5
34	728644.0
35	781250.5
36	838236.5
37	890083.5
38	929059.0
39	961345.5
40	998279.0
41	1036989.5
42	1063614.5
43	1090251.5
44	1118084.5
45	1140595.0
46	1152676.0
47	1147297.0
48	1125420.5
49	1092907.0
50	1058575.0
51	1017092.5
52	961255.5
53	908850.0
54	855518.5
55	789528.5
56	721964.5
57	651955.5
58	575647.5
59	496557.0
60	418772.0
61	343320.0
62	273544.5
63	213174.5
64	161697.0
65	122043.5
66	93151.0
67	71482.0
68	54475.0
69	40007.5
70	29063.5
71	21051.5
72	15385.5
73	11219.5
74	8096.0
75	5695.5
76	3851.5
77	2514.0
78	1675.5
79	1157.5
80	815.5
81	588.0
82	432.5
83	299.5
84	205.5
85	142.0
86	102.0
87	83.5
88	64.0
89	45.5
90	29.5
91	16.0
92	10.5
93	8.0
94	5.5
95	5.0
96	4.5
97	3.5
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0010495112730822544
3	0.0
4	0.0019523996477191939
5	3.2025527818318794E-4
6	3.665572461132874E-4
7	0.0
8	0.0
9	0.0
10-11	2.6816556426182607E-4
12-13	4.8809991192979847E-4
14-15	1.1575491982524866E-5
16-17	0.0
18-19	0.0010282895377809588
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.4073330465644374E-5
28-29	0.0
30-31	5.419454032200577E-5
32-33	8.286423025776695E-4
34-35	6.718155780946762E-4
36-37	3.620119245162479E-4
38-39	0.0015490805477738018
40-41	3.972567041041385E-6
42-43	1.8027094723369223E-5
44-45	1.2134288226314505E-5
46-47	1.4313727418740306E-5
48-49	6.20179384406222E-5
50-51	2.5065840652523994E-5
52-53	6.327453968563311E-6
54-55	0.003070169359441526
56-57	0.002869144163331645
58-59	0.0014716121878877927
60-61	1.7802328091763835E-4
62-63	1.7777738666752713E-5
64-65	1.1457749470197034E-4
66-67	1.818676994327092E-5
68-69	1.4731980164125308E-4
70-71	0.0
72-73	0.0
74-75	1.3631050135624165E-4
76-77	2.422046200821926E-6
78-79	3.8763662903140255E-4
80-81	0.0
82-83	1.3873493165223594E-4
84-85	1.5343902895599065E-5
86-87	0.0015093600551958969
88-89	0.0
90-91	9.633997220110101E-5
92-93	1.3614365420949509E-5
94-95	1.8587263136194508E-4
96-97	8.510793061513544E-6
98-99	5.938344543938108E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1052.0
20-21	4714.0
22-23	6727.0
24-25	10342.0
26-27	16810.0
28-29	29396.0
30-31	64104.0
32-33	81818.0
34-35	110838.0
36-37	170041.0
38-39	198430.0
40-41	206203.0
42-43	228270.0
44-45	267098.0
46-47	269691.0
48-49	253801.0
50-51	237425.0
52-53	218618.0
54-55	233505.0
56-57	244998.0
58-59	247737.0
60-61	256048.0
62-63	240023.0
64-65	257648.0
66-67	271055.0
68-69	278042.0
70-71	276646.0
72-73	262424.0
74-75	266236.0
76-77	258508.0
78-79	275778.0
80-81	282649.0
82-83	271609.0
84-85	269867.0
86-87	283374.0
88-89	303137.0
90-91	318284.0
92-93	331714.0
94-95	372580.0
96-97	568737.0
98-99	2181939.0
100-101	1.4988909E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.80339324527144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.92465838342017	71.04610747311652
2	7.671581007330369	12.397795538965427
3	2.1796404996032215	5.28367045268277
4	0.9555963117821444	3.0886169825865446
5	0.4669454621489781	1.8865388901059443
6	0.24277352500497576	1.1770154766310676
7	0.1559912821926751	0.8823237442494183
8	0.0973479394897634	0.6292835068966592
9	0.07755518511871273	0.5640049909221452
>10	0.22412625550043774	2.7027203628717573
>50	0.0028764006644732493	0.16097922662065356
>100	8.509755516845985E-4	0.1057019668785791
>500	1.8988668178828888E-5	0.01057097548503475
>1k	3.778352429689454E-5	0.06467041198746729
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1575491982524866E-5
2	0.0	5.787745991262433E-5	0.0	0.0	1.5433989310033156E-5
3	0.0	6.173595724013262E-5	0.0	0.0	1.5433989310033156E-5
4	0.0	1.0803792517023207E-4	0.0	0.0	1.9292486637541442E-5
5	0.0	1.1189642249774037E-4	0.0	0.0	1.9292486637541442E-5
6	7.716994655016578E-6	1.1575491982524866E-4	0.0	3.858497327508289E-6	2.700948129255802E-5
7	7.716994655016578E-6	1.1961341715275694E-4	0.0	1.5433989310033156E-5	2.700948129255802E-5
8	7.716994655016578E-6	1.4662289844531495E-4	0.0	2.700948129255802E-5	2.700948129255802E-5
9	7.716994655016578E-6	2.3922683430551388E-4	0.0	5.016046525760775E-5	3.086797862006631E-5
10-11	7.716994655016578E-6	2.469438289605305E-4	0.0	1.389059037902984E-4	3.086797862006631E-5
12-13	7.716994655016578E-6	2.469438289605305E-4	0.0	2.3343908831425145E-4	3.086797862006631E-5
14-15	7.716994655016578E-6	3.3954776482072937E-4	0.0	3.9163747874209124E-4	5.20897139213619E-5
16-17	2.3150983965049732E-5	3.684864947770415E-4	1.9292486637541445E-6	6.906710216239837E-4	6.55944545676409E-5
18-19	2.3150983965049732E-5	3.9742522473335375E-4	3.858497327508289E-6	9.549780885583014E-4	8.102844387767406E-5
20-21	2.3150983965049732E-5	4.225054573621576E-4	3.858497327508289E-6	0.0014083515245405253	8.29576925414282E-5
22-23	2.5080232628803875E-5	4.6301967930099464E-4	3.858497327508289E-6	0.0021048102921557713	1.0225017917896964E-4
24-25	2.700948129255802E-5	5.170386418861107E-4	3.858497327508289E-6	0.002699018880592048	1.2733041180777353E-4
26-27	2.700948129255802E-5	5.47906620506177E-4	3.858497327508289E-6	0.003194835787176863	1.350474064627901E-4
28-29	3.086797862006631E-5	5.61411361152456E-4	3.858497327508289E-6	0.0045665315871060595	1.350474064627901E-4
30-31	3.086797862006631E-5	6.270058157200969E-4	3.858497327508289E-6	0.007522140539977408	1.3697665512654424E-4
32-33	3.279722728382046E-5	6.385813077026217E-4	3.858497327508289E-6	0.013037862469650507	1.4276440111780667E-4
34-35	3.8584973275082884E-5	7.620532221828871E-4	3.858497327508289E-6	0.02004103511907805	1.5626914176408566E-4
36-37	3.8584973275082884E-5	8.700911473531191E-4	3.858497327508289E-6	0.029945797758791824	1.5819839042783982E-4
38-39	3.8584973275082884E-5	8.932421313181688E-4	3.858497327508289E-6	0.049107095487197994	1.6398613641910228E-4
40-41	3.8584973275082884E-5	9.74270575195843E-4	3.858497327508289E-6	0.09336020133639056	1.659153850828564E-4
42-43	3.8584973275082884E-5	0.0010205725431259422	3.858497327508289E-6	0.1101871081816542	1.6977388241036468E-4
44-45	4.051422193883703E-5	0.0011594784469162408	3.858497327508289E-6	0.13346349330984794	1.7942012572913542E-4
46-47	4.244347060259117E-5	0.001282950361396506	3.858497327508289E-6	0.15971477987755056	1.9485411503916857E-4
48-49	4.437271926634532E-5	0.0012925966047152766	3.858497327508289E-6	0.1826265370082948	2.083588556854476E-4
50-51	5.401896258511604E-5	0.001417997767859296	3.858497327508289E-6	0.2064778382382873	2.083588556854476E-4
52-53	5.787745991262433E-5	0.0014295732598418208	3.858497327508289E-6	0.2387252296529378	2.218635963317266E-4
54-55	5.787745991262433E-5	0.0014507949951431164	3.858497327508289E-6	0.3040727403916182	2.353683369780056E-4
56-57	5.787745991262433E-5	0.0014739459791081662	3.858497327508289E-6	0.3750536572284606	2.3922683430551388E-4
58-59	5.787745991262433E-5	0.0015067432063919867	3.858497327508289E-6	0.45023261915763213	2.4694382896053044E-4
60-61	6.559445456764092E-5	0.001551115925658332	3.858497327508289E-6	0.5000149516771442	2.5466082361554705E-4
62-63	8.488694120518234E-5	0.001562691417640857	3.858497327508289E-6	0.5206984266012523	2.5851932094305536E-4
64-65	8.488694120518234E-5	0.0016861633321211221	3.858497327508289E-6	0.5404616499127497	2.5851932094305536E-4
66-67	8.488694120518234E-5	0.0017131728134136802	3.858497327508289E-6	0.5487728531562026	2.6430706693431776E-4
68-69	8.488694120518234E-5	0.0017459700406975006	3.858497327508289E-6	0.5559612336773505	2.739533102530885E-4
70-71	9.067468719644477E-5	0.001778767267981321	3.858497327508289E-6	0.563145755701171	2.9517504555438406E-4
72-73	1.0610867650647793E-4	0.0018019182519463707	3.858497327508289E-6	0.5686749823714903	3.4340626214823767E-4
74-75	1.0803792517023207E-4	0.0018289277332389287	3.858497327508289E-6	0.5731083958007974	3.4340626214823767E-4
76-77	1.0996717383398623E-4	0.001911885425780357	3.858497327508289E-6	0.5769630346309782	3.453355108119918E-4
78-79	1.1189642249774037E-4	0.0019427534044004233	3.858497327508289E-6	0.5803392197925479	3.47264759475746E-4
80-81	1.1189642249774037E-4	0.0019446826530641775	3.858497327508289E-6	0.5819771519080752	3.60769500122025E-4
82-83	1.1189642249774037E-4	0.0019639751397017187	3.858497327508289E-6	0.5825482095125464	3.8970823007833714E-4
84-85	1.1189642249774037E-4	0.00200641861030431	3.858497327508289E-6	0.5831983663122315	3.8970823007833714E-4
86-87	1.1189642249774037E-4	0.0020488620809069012	3.858497327508289E-6	0.5834626733791659	3.8970823007833714E-4
88-89	1.1189642249774037E-4	0.0021337490221120838	3.858497327508289E-6	0.5835629943096811	3.8970823007833714E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	41920	0.0	29.09777	1
GTATCAA	63355	0.0	24.916134	1
TCAACGC	60225	0.0	24.759672	4
ATCAACG	60495	0.0	24.649122	3
CAACGCA	60980	0.0	24.42508	5
AACGCAG	62820	0.0	23.776094	6
GTACATG	68230	0.0	21.082767	1
TATCAAC	74110	0.0	21.006844	2
TACATGG	68130	0.0	20.579735	2
ACGCAGA	73065	0.0	20.384998	7
CGCAGAG	73815	0.0	20.143887	8
ACATGGG	68020	0.0	19.99802	3
CATGGGG	49585	0.0	17.869856	4
AGAGTAC	71860	0.0	16.796164	10-11
GAGTACT	46080	0.0	16.719677	12-13
GCAGAGT	88835	0.0	16.67211	9
CAGAGTA	77675	0.0	16.556181	10-11
GTACTTT	52010	0.0	14.866692	14-15
AGTACTT	50260	0.0	14.605325	12-13
GAGTACA	50110	0.0	14.5284395	1
>>END_MODULE