FastQCFastQC Report
Fri 27 May 2016
SRR2138827_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138827_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24282516
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA249250.042.7047921
GTATCAA432750.033.0474661
TCAACGC495550.028.0321834
ATCAACG501550.027.6142563
CAACGCA510600.027.1655945
AACGCAG526000.026.3862196
TATCAAC537600.026.0630872
ACGCAGA629750.022.0259917
CGCAGAG643200.021.5911648
GTACATG660300.021.4141521
TACATGG654050.021.1757342
ACATGGG676650.019.9113773
CATGGGG493750.017.9006424
GCAGAGT782700.017.5575969
GAGTACT404600.017.15649612-13
AGAGTAC664300.016.96478310-11
CAGAGTA744200.016.13401810-11
GAGTACA480150.014.5863071
GTGGTAT151450.014.4665531
GTACTTT483300.014.37652414-15
Produced by FastQC (version 0.11.5)