##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138827_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24282516
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.595344362174004	31.0	31.0	34.0	30.0	34.0
2	31.79841817050589	31.0	31.0	34.0	30.0	34.0
3	31.858145136195937	31.0	31.0	34.0	30.0	34.0
4	35.5902146219115	37.0	35.0	37.0	33.0	37.0
5	35.32376278471308	37.0	35.0	37.0	33.0	37.0
6	35.3473119507056	37.0	35.0	37.0	33.0	37.0
7	35.30566638975961	37.0	35.0	37.0	32.0	37.0
8	35.29064554101395	37.0	35.0	37.0	32.0	37.0
9	37.0387305005791	39.0	37.0	39.0	33.0	39.0
10-11	36.872559787461896	39.0	37.0	39.0	33.0	39.0
12-13	36.87601482482293	39.0	37.0	39.0	33.0	39.0
14-15	37.950802112104036	40.0	37.0	41.0	33.0	41.0
16-17	37.86951687790507	39.0	37.0	41.0	33.0	41.0
18-19	37.92553220185256	39.5	37.0	41.0	33.0	41.0
20-21	37.96491744741421	40.0	37.0	41.0	33.0	41.0
22-23	37.946096799471306	40.0	37.0	41.0	33.0	41.0
24-25	37.89745715127897	39.0	37.0	41.0	33.0	41.0
26-27	37.76421520569343	39.0	37.0	41.0	33.0	41.0
28-29	37.589040067107845	39.0	37.0	40.5	32.0	41.0
30-31	37.48453493127699	39.0	37.0	40.0	32.0	41.0
32-33	37.386150223070516	39.0	36.0	40.0	32.0	41.0
34-35	37.313389923478184	39.0	36.0	40.0	31.0	41.0
36-37	37.266879276860024	39.0	36.0	40.0	31.0	41.0
38-39	37.18078917543227	39.0	36.0	40.0	31.0	41.0
40-41	37.1605242955628	39.0	36.0	40.0	31.0	41.0
42-43	37.105372298385674	39.0	36.0	40.0	31.0	41.0
44-45	37.03250097608626	39.0	36.0	40.0	31.0	41.0
46-47	36.97177043481855	39.0	35.5	40.0	31.0	41.0
48-49	36.98155694885471	39.0	36.0	40.0	31.0	41.0
50-51	36.86843443898418	39.0	35.0	40.0	31.0	41.0
52-53	36.67259248013039	39.0	35.0	40.0	30.0	41.0
54-55	36.46919002511848	38.0	35.0	40.0	30.0	41.0
56-57	36.3435721022548	38.0	35.0	40.0	30.0	41.0
58-59	36.14876833783653	38.0	34.0	40.0	29.5	41.0
60-61	35.967420606087536	38.0	34.0	40.0	29.0	41.0
62-63	35.82168895133175	37.5	34.0	40.0	29.0	41.0
64-65	35.56397567025747	37.0	34.0	40.0	29.0	41.0
66-67	35.424451785731705	37.0	34.0	39.0	29.0	41.0
68-69	35.157619349249245	36.0	34.0	39.0	29.0	40.5
70-71	34.8413150135679	36.0	33.0	39.0	28.0	40.0
72-73	34.55112424790185	35.0	33.0	38.5	28.0	40.0
74-75	34.206155808711145	35.0	33.0	37.5	28.0	39.5
76-77	32.086928411270456	33.5	30.0	35.5	25.5	38.5
78-79	33.37669311805645	34.5	32.0	36.0	27.0	39.0
80-81	33.40350561102265	35.0	32.0	36.0	27.5	38.5
82-83	33.252341281454235	35.0	32.0	36.0	27.5	37.5
84-85	33.06234339486002	34.5	32.0	35.5	27.0	37.0
86-87	32.83099893595829	34.0	32.0	35.0	27.0	36.5
88-89	32.68431209412299	34.0	32.0	35.0	27.0	36.0
90-91	32.53135431213113	34.0	32.0	35.0	27.0	36.0
92-93	32.5107191762787	34.0	32.0	35.0	27.0	36.0
94-95	32.491087627950606	34.0	32.0	35.0	27.0	35.0
96-97	32.53866213928702	34.0	32.0	35.0	27.0	35.0
98-99	32.70875074272886	34.0	32.0	35.0	29.0	35.0
100-101	31.55050082778399	33.0	29.5	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	9.0
19	34.0
20	245.0
21	1353.0
22	5869.0
23	18802.0
24	46656.0
25	97302.0
26	172546.0
27	270569.0
28	387061.0
29	520836.0
30	673522.0
31	851762.0
32	1081473.0
33	1394347.0
34	1871503.0
35	2598149.0
36	3821417.0
37	5094606.0
38	4166638.0
39	1198710.0
40	9104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.340374284114546	20.660532047008637	13.76731719233707	24.231776476539746
2	15.342104118730775	21.81444593358688	39.81810800499719	23.02534194268515
3	18.625847710093485	26.091906772604247	30.375403233179348	24.90684228412292
4	12.937596357938135	17.960055116215546	38.34854133071669	30.753807195129628
5	13.09105749548595	38.00674271178253	35.8200787686119	13.082121024119619
6	30.485469462884325	38.0995363083463	18.354733092732236	13.060261136037138
7	26.74673113555165	32.38209057010775	23.235999236487654	17.635179057852945
8	24.7680858753533	36.82509176244851	21.082165155604933	17.32465720659326
9	25.684933142842365	16.256017292442017	20.98825755947201	37.07079200524361
10-11	24.421478700673756	26.650871031467116	28.98869354041406	19.93895672744507
12-13	25.01283747930344	24.081255921208825	29.17285204128173	21.73305455820601
14-15	22.058883848774155	25.574930126680446	26.433729107807448	25.93245691673795
16-17	21.92126676496922	28.73908183029884	27.144354134150557	22.195297270581378
18-19	22.00036520231438	28.092372629412598	28.57558413323608	21.331678035036937
20-21	22.666073886395406	27.308552427282827	28.416720686823393	21.608652999498375
22-23	22.408767286919094	27.204852179464734	28.314887087971496	22.071493445644673
24-25	22.209152651356227	27.3335213252972	28.346541595426324	22.11078442792025
26-27	22.14834397114056	27.536646109008277	28.27882786122683	22.036182058624334
28-29	22.147291337237867	27.623444783229832	28.218516439021315	22.010747440510986
30-31	22.369923435589644	27.43127344251951	28.16596091680624	22.032842205084602
32-33	22.16520987338373	27.606170920755464	28.14344676654193	22.085172439318875
34-35	22.264811478709497	27.606403896213898	28.13414572616001	21.994638898916598
36-37	22.307146670909685	27.5664167418905	28.087928251188494	22.038508336011322
38-39	22.162225756523103	27.667996284847902	28.13445137297719	22.03532658565181
40-41	22.334031407218745	27.555005121064823	28.011270498146416	22.099692973570015
42-43	22.25215687963153	27.624667687601733	27.88054786010507	22.24262757266166
44-45	22.30388563093384	27.71037427231473	27.737799405339686	22.247940691411742
46-47	22.373129580266575	27.701332575383898	27.566445119607195	22.35909272474233
48-49	22.30772215447109	27.80662982437145	27.54580974744867	22.339838273708793
50-51	22.279073947044257	27.882157590194943	27.497880999996095	22.34088746276471
52-53	22.470354362112037	27.765895091775057	27.384261792389996	22.379488753722914
54-55	22.399870322014287	27.68765889856471	27.451774004191964	22.460696775229042
56-57	22.391705647554648	27.77513914958028	27.431559312556164	22.401595890308904
58-59	22.383659740449424	27.83154212070336	27.3729333832141	22.41186475563311
60-61	22.467115909227402	27.751357391044788	27.43861755748247	22.342909142245336
62-63	22.431726258641795	27.835730114032586	27.371509102248474	22.361034525077145
64-65	22.40647871576217	27.778434448363214	27.363274445235085	22.45181239063953
66-67	22.45318943875592	27.807913864947416	27.250207273310345	22.488689422986322
68-69	22.386750134598827	27.817153353255	27.314949423312335	22.481147088833833
70-71	22.383523104532788	27.63013471257785	27.396489204650535	22.58985297823883
72-73	22.43994598134328	27.70878280896094	27.315644813061702	22.53562639663408
74-75	22.293297924694933	27.802937547936235	27.33395199920346	22.56981252816537
76-77	22.414600638665906	27.72901779257957	27.28606334591009	22.57031822284444
78-79	22.380384535590494	27.764415269498848	27.23653935628531	22.618660838625345
80-81	22.399324066461197	27.775896241861425	27.257214441199135	22.56756525047824
82-83	22.380761463056608	27.768972827499123	27.327162849123678	22.523102860320595
84-85	22.463700548557703	27.749496740728574	27.277249076392835	22.509553634320888
86-87	22.33464613090846	27.769273698771553	27.3595974820707	22.536482688249286
88-89	22.36856267595767	27.74095066904981	27.320994202775182	22.569492452217343
90-91	22.43418302781895	27.771395533068695	27.325460968961384	22.46896047015097
92-93	22.385016130533568	27.73467763112357	27.347718545143962	22.532587693198902
94-95	22.23542997157326	27.788199797699786	27.410765995254128	22.565604235472826
96-97	22.24577306748226	27.808660400478818	27.434572185569657	22.51099434646926
98-99	22.415347005704135	28.407478199139796	27.523795557673754	21.653379237482316
100-101	26.575772866707194	35.49444126355266	28.049279891658657	9.880505978081494
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4801.0
1	4262.5
2	5606.0
3	8040.5
4	10543.0
5	16499.5
6	21847.0
7	25638.5
8	28478.5
9	28403.0
10	26159.0
11	23548.0
12	24459.5
13	31218.5
14	48230.5
15	71805.0
16	91159.0
17	103246.0
18	107955.0
19	106578.5
20	102882.0
21	100115.0
22	102164.0
23	113755.5
24	136418.5
25	169599.5
26	212781.0
27	261551.5
28	315321.5
29	373573.0
30	429532.5
31	485621.0
32	542108.5
33	592874.0
34	639707.0
35	685161.0
36	736503.5
37	786923.0
38	827821.0
39	863720.0
40	900776.0
41	937552.0
42	966847.0
43	993442.0
44	1019560.0
45	1038829.0
46	1050144.5
47	1056581.5
48	1053975.0
49	1040839.0
50	1023921.0
51	998818.0
52	959674.5
53	916146.5
54	869970.0
55	814415.0
56	752905.0
57	687647.0
58	615320.5
59	535890.5
60	453856.0
61	375054.5
62	303311.5
63	239056.5
64	181920.5
65	138215.5
66	106582.0
67	82850.0
68	64160.5
69	47771.5
70	35351.5
71	26338.5
72	19348.0
73	14113.5
74	10298.5
75	7390.0
76	5233.5
77	3642.0
78	2532.5
79	1776.5
80	1250.0
81	897.0
82	672.5
83	502.5
84	355.5
85	233.0
86	159.5
87	118.0
88	78.5
89	49.0
90	36.0
91	27.0
92	22.0
93	17.0
94	10.0
95	7.0
96	7.0
97	5.0
98	1.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.4592790961200236E-4
3	1.6472757600571538E-4
4	2.8827325801000192E-5
5	4.118189400142885E-6
6	0.0
7	4.118189400142885E-6
8	0.001037783728836007
9	0.0
10-11	2.862141633099305E-4
12-13	4.159371294144314E-4
14-15	0.0
16-17	2.0590947000714423E-6
18-19	3.5004609901214523E-4
20-21	0.0
22-23	0.0
24-25	1.1536606331726299E-4
26-27	0.0
28-29	4.290377006153473E-4
30-31	3.057435516877343E-4
32-33	8.65754672134149E-4
34-35	0.0010514786054235102
36-37	5.429058946132794E-5
38-39	0.0015184429406578197
40-41	3.4346677639511433E-4
42-43	1.17592835320834E-4
44-45	1.1227407622023622E-4
46-47	3.057186018370281E-5
48-49	4.416711520212451E-6
50-51	4.465165092669924E-6
52-53	1.1279245052156132E-5
54-55	3.1005892008621186E-4
56-57	5.212647414130168E-4
58-59	4.1556757215105195E-4
60-61	4.1361904277492146E-4
62-63	5.02377549598958E-5
64-65	4.8423043073507394E-5
66-67	1.1526751886641115E-4
68-69	7.210424324271976E-5
70-71	9.58265422575886E-5
72-73	0.0
74-75	1.0371597752931118E-5
76-77	6.785263071355641E-4
78-79	1.600601868985455E-5
80-81	1.3272914305081132E-4
82-83	8.254067005800601E-6
84-85	5.5884318343342915E-6
86-87	5.679377179052787E-6
88-89	1.73369732886652E-5
90-91	5.595012994123204E-5
92-93	3.3060827956139946E-5
94-95	2.1826708599360434E-4
96-97	4.110093469532557E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	956.0
20-21	2736.0
22-23	5345.0
24-25	11351.0
26-27	15837.0
28-29	29251.0
30-31	59418.0
32-33	73772.0
34-35	102063.0
36-37	159504.0
38-39	191673.0
40-41	193468.0
42-43	217442.0
44-45	256570.0
46-47	259288.0
48-49	248556.0
50-51	234835.0
52-53	228062.0
54-55	249758.0
56-57	260571.0
58-59	261842.0
60-61	257692.0
62-63	247927.0
64-65	259261.0
66-67	275558.0
68-69	281812.0
70-71	279975.0
72-73	267170.0
74-75	274979.0
76-77	262913.0
78-79	281970.0
80-81	288101.0
82-83	278788.0
84-85	283215.0
86-87	298584.0
88-89	319122.0
90-91	340006.0
92-93	360314.0
94-95	417309.0
96-97	617501.0
98-99	2059043.0
100-101	1.3268978E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.91623722910708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.03664909143905	73.82583918150522
2	7.271678657907687	12.058804653058179
3	1.9125327075201635	4.75740047055505
4	0.7659325621133587	2.540329840867562
5	0.37370484816335425	1.5493099921990061
6	0.19653830207521664	0.9777729887684742
7	0.12733329709221303	0.7390598508203614
8	0.08131116141144198	0.5393612439172273
9	0.049156440143271585	0.36682803470321185
>10	0.18058481415126873	2.30669828749848
>50	0.003637609798807856	0.20121454637544875
>100	9.116536416559906E-4	0.11186029189458031
>500	1.9247276164241375E-5	0.012679452000555704
>1k	9.607266364087049E-6	0.012841165836721949
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.23637880028577E-6
2	0.0	2.4709136400857308E-5	0.0	0.0	8.23637880028577E-6
3	0.0	2.4709136400857308E-5	0.0	8.23637880028577E-6	8.23637880028577E-6
4	0.0	8.648197740300058E-5	0.0	1.647275760057154E-5	1.2354568200428654E-5
5	0.0	8.648197740300058E-5	0.0	1.647275760057154E-5	1.647275760057154E-5
6	4.530008340157173E-5	8.648197740300058E-5	0.0	2.8827325801000192E-5	3.706370460128596E-5
7	4.530008340157173E-5	9.060016680314346E-5	0.0	3.294551520114308E-5	4.530008340157173E-5
8	4.530008340157173E-5	1.1530930320400077E-4	0.0	6.177284100214327E-5	5.7654651602000385E-5
9	4.530008340157173E-5	2.182640382075729E-4	0.0	1.07072924403715E-4	8.648197740300058E-5
10-11	5.147736750178606E-5	2.182640382075729E-4	0.0	1.420775343049295E-4	8.648197740300058E-5
12-13	5.7654651602000385E-5	2.2238222760771577E-4	0.0	1.461957237050724E-4	9.060016680314346E-5
14-15	6.177284100214327E-5	3.150414891109307E-4	0.0	1.5649119720542962E-4	1.2560477670435797E-4
16-17	1.1942749260414365E-4	3.39750625511788E-4	0.0	1.8120033360628692E-4	1.5443210250535818E-4
18-19	1.1942749260414365E-4	3.58282477812431E-4	0.0	2.0179128060700136E-4	1.6678667070578685E-4
20-21	1.1942749260414365E-4	3.747552354130025E-4	0.0	2.3473679580814444E-4	1.750230495060726E-4
22-23	1.2972296610450087E-4	4.3035079231493146E-4	0.0	2.862141633099305E-4	2.038503753070728E-4
24-25	1.359002502047152E-4	4.880054439169319E-4	0.0	3.58282477812431E-4	2.3473679580814444E-4
26-27	1.359002502047152E-4	5.106554856177177E-4	0.0	4.2417350821471713E-4	2.3473679580814444E-4
28-29	1.359002502047152E-4	5.188918644180034E-4	0.0	8.071651224280054E-4	2.4091407990835874E-4
30-31	1.359002502047152E-4	5.930192736205754E-4	0.0	0.0019334899233670842	2.532686481087874E-4
32-33	1.400184396048581E-4	5.971374630207183E-4	0.0	0.0038752162255344543	2.8209597390978756E-4
34-35	1.4825481840514385E-4	6.939149139240761E-4	0.0	0.0064346709377232575	3.0062782621043056E-4
36-37	1.4825481840514385E-4	7.948105542275768E-4	0.0	0.010423137371761641	3.068051103106449E-4
38-39	1.503139131052153E-4	8.215787853285055E-4	0.0	0.01678779908968247	3.1915967851107356E-4
40-41	1.5237300780528674E-4	8.874698157307916E-4	0.0	0.023675470861421445	3.335733414115737E-4
42-43	1.6472757600571538E-4	9.08060762731506E-4	0.0	0.029628313639327983	3.500460990121452E-4
44-45	1.6884576540585826E-4	0.0010398428235360783	0.0	0.03663747199837117	3.644597619126453E-4
46-47	1.7296395480600115E-4	0.0011489748426398649	0.0	0.04410786757023037	3.788734248131454E-4
48-49	1.7296395480600115E-4	0.0011530930320400078	0.0	0.051273517126478985	3.994643718138598E-4
50-51	1.853185230064298E-4	0.0012436931988431513	0.0	0.058552416891231536	3.994643718138598E-4
52-53	1.894367124065727E-4	0.0012560477670435798	0.0	0.06544420685237065	4.2829169761486E-4
54-55	1.894367124065727E-4	0.001266343240543937	0.0	0.07287342053022841	4.715326863163603E-4
56-57	1.935549018067156E-4	0.0013013478504451516	0.0	0.08003907008647704	4.8388725451678895E-4
58-59	1.935549018067156E-4	0.001303406945145223	0.0	0.08726855157842786	4.859463492168604E-4
60-61	2.244413223077872E-4	0.0013301751762461516	0.0	0.09404503223636299	4.941827280171462E-4
62-63	2.429731746084302E-4	0.0013548843126470092	0.0	0.10062383980309125	5.106554856177177E-4
64-65	2.4503226930850167E-4	0.001503139131052153	0.0	0.107188233706919	5.106554856177177E-4
66-67	2.553277428088589E-4	0.001507257320452296	0.0	0.11377733674714763	5.127145803177891E-4
68-69	2.553277428088589E-4	0.001531966456853153	0.0	0.12082767700019223	5.147736750178606E-4
70-71	2.656232163092161E-4	0.0015525574038538674	0.0	0.12709967945660985	5.16832769717932E-4
72-73	2.923914474101448E-4	0.0015649119720542962	0.0	0.13239773011989367	5.477191902190036E-4
74-75	2.923914474101448E-4	0.0015978574872554394	0.0	0.13672594717944386	5.497782849190751E-4
76-77	3.0474601561057343E-4	0.001657571233557511	0.0	0.14040349031377142	5.559555690192895E-4
78-79	3.0886420501071636E-4	0.0016822803699583684	0.0	0.14408721073219924	5.600737584194323E-4
80-81	3.0886420501071636E-4	0.0016863985593585113	0.0	0.1457921411438584	5.744874213199323E-4
82-83	3.1298239441085923E-4	0.0016946349381587972	0.0	0.14650252881538306	6.09492031221147E-4
84-85	3.171005838110021E-4	0.001709048601059297	0.0	0.14733852126361208	6.09492031221147E-4
86-87	3.1915967851107356E-4	0.0017234622639597974	0.0	0.14770709921492486	6.136102206212898E-4
88-89	3.3357334141157364E-4	0.0017749396314615832	0.0	0.14782240851812883	6.218465994215756E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	24925	0.0	42.704792	1
GTATCAA	43275	0.0	33.047466	1
TCAACGC	49555	0.0	28.032183	4
ATCAACG	50155	0.0	27.614256	3
CAACGCA	51060	0.0	27.165594	5
AACGCAG	52600	0.0	26.386219	6
TATCAAC	53760	0.0	26.063087	2
ACGCAGA	62975	0.0	22.025991	7
CGCAGAG	64320	0.0	21.591164	8
GTACATG	66030	0.0	21.414152	1
TACATGG	65405	0.0	21.175734	2
ACATGGG	67665	0.0	19.911377	3
CATGGGG	49375	0.0	17.900642	4
GCAGAGT	78270	0.0	17.557596	9
GAGTACT	40460	0.0	17.156496	12-13
AGAGTAC	66430	0.0	16.964783	10-11
CAGAGTA	74420	0.0	16.134018	10-11
GAGTACA	48015	0.0	14.586307	1
GTGGTAT	15145	0.0	14.466553	1
GTACTTT	48330	0.0	14.376524	14-15
>>END_MODULE