FastQCFastQC Report
Fri 27 May 2016
SRR2138826_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138826_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12418463
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127850.040.0161971
GTATCAA219950.032.256111
TCAACGC241250.028.3506034
ATCAACG242500.028.1527163
CAACGCA247500.027.6516915
AACGCAG258000.026.5912976
TATCAAC265150.026.1896722
ACGCAGA306550.022.298017
CGCAGAG309300.022.0727088
GTACATG315850.022.0385281
TACATGG317350.021.4731732
ACATGGG326600.020.3397773
CATGGGG235150.018.2877394
GCAGAGT376850.017.9495439
AGAGTAC327000.017.15508710-11
GAGTACT211100.017.0222112-13
CAGAGTA361500.016.26426510-11
GAGTACA224700.015.6568021
ATGGGGG147050.015.3333635
GTACTTT247400.014.72849314-15
Produced by FastQC (version 0.11.5)