##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138826_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12418463
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.693408516013616	31.0	31.0	34.0	30.0	34.0
2	31.890376127867032	31.0	31.0	34.0	30.0	34.0
3	31.954279366134116	33.0	31.0	34.0	30.0	34.0
4	35.66590253560364	37.0	35.0	37.0	33.0	37.0
5	35.41480390930826	37.0	35.0	37.0	33.0	37.0
6	35.44109371667009	37.0	35.0	37.0	33.0	37.0
7	35.39846976232083	37.0	35.0	37.0	33.0	37.0
8	35.38617355465004	37.0	35.0	37.0	33.0	37.0
9	37.14534954929608	39.0	37.0	39.0	33.0	39.0
10-11	36.98788420918112	39.0	37.0	39.0	33.0	39.0
12-13	36.99139088307466	39.0	37.0	39.0	33.0	39.0
14-15	38.090448189924956	40.0	37.0	41.0	33.0	41.0
16-17	38.01539373270268	40.0	37.0	41.0	33.0	41.0
18-19	38.073432356322996	40.0	37.0	41.0	33.0	41.0
20-21	38.11350922096595	40.0	37.0	41.0	33.0	41.0
22-23	38.09847750798531	40.0	37.0	41.0	33.0	41.0
24-25	38.05089046286935	40.0	37.0	41.0	33.0	41.0
26-27	37.926041759866834	40.0	37.0	41.0	33.0	41.0
28-29	37.75242795518684	39.5	37.0	41.0	33.0	41.0
30-31	37.65329530630401	39.0	37.0	41.0	32.0	41.0
32-33	37.56240666343528	39.0	37.0	40.5	32.0	41.0
34-35	37.49160899047446	39.0	37.0	40.0	32.0	41.0
36-37	37.44671221275415	39.0	36.0	40.0	32.0	41.0
38-39	37.362757519127	39.0	36.0	40.0	31.5	41.0
40-41	37.34383021189596	39.0	36.0	40.0	31.5	41.0
42-43	37.29144496512539	39.0	36.0	40.0	31.0	41.0
44-45	37.21935135024937	39.0	36.0	40.0	31.0	41.0
46-47	37.1631150955574	39.0	36.0	40.0	31.0	41.0
48-49	37.177013148193	39.0	36.0	40.0	31.0	41.0
50-51	37.071659893876316	39.0	36.0	40.0	31.0	41.0
52-53	36.8827196611875	39.0	35.0	40.0	31.0	41.0
54-55	36.689725676253204	39.0	35.0	40.0	30.5	41.0
56-57	36.57221578488342	38.0	35.0	40.0	30.0	41.0
58-59	36.384501296459696	38.0	35.0	40.0	30.0	41.0
60-61	36.208427383725436	38.0	34.0	40.0	30.0	41.0
62-63	36.06914746906016	38.0	34.0	40.0	29.5	41.0
64-65	35.816538952325075	37.0	34.0	40.0	29.0	41.0
66-67	35.6822075044656	37.0	34.0	39.5	29.0	41.0
68-69	35.417880089885	36.5	34.0	39.0	29.0	41.0
70-71	35.10009458626705	36.0	34.0	39.0	29.0	40.0
72-73	34.80909234379003	36.0	33.0	39.0	29.0	40.0
74-75	34.4623251000174	35.0	33.0	38.0	28.5	40.0
76-77	32.3294416820293	33.5	30.0	35.5	25.5	38.5
78-79	33.61563312577417	35.0	32.0	37.0	28.0	39.0
80-81	33.62898594066007	35.0	32.5	36.0	28.0	39.0
82-83	33.467048639451065	35.0	32.5	36.0	28.0	37.5
84-85	33.27045173733705	35.0	32.0	35.5	28.0	37.0
86-87	33.03019154945607	34.5	32.0	35.0	28.0	37.0
88-89	32.875178608232545	34.0	32.0	35.0	27.5	36.0
90-91	32.71573517049308	34.0	32.0	35.0	27.0	36.0
92-93	32.685819470225596	34.0	32.0	35.0	27.5	36.0
94-95	32.65345041586539	34.0	32.0	35.0	27.5	35.5
96-97	32.688044179095606	34.0	32.0	35.0	28.5	35.0
98-99	32.84278381018426	34.0	32.0	35.0	29.0	35.0
100-101	31.684734262288185	33.0	30.5	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	22.0
20	110.0
21	581.0
22	2595.0
23	8332.0
24	21269.0
25	44479.0
26	80407.0
27	126834.0
28	182979.0
29	247269.0
30	321038.0
31	407623.0
32	518034.0
33	671553.0
34	906627.0
35	1266921.0
36	1899056.0
37	2669385.0
38	2336261.0
39	700496.0
40	6588.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.312028710799396	20.67161612511951	13.648927407522171	24.367427756558925
2	15.10208725829004	21.79243599575984	40.08350997987179	23.021966766078325
3	18.4640669584638	26.133948332500566	30.453273336557036	24.9487113724786
4	12.65278362592595	17.943350629357376	38.7555189004499	30.648346844266772
5	12.893689017714994	38.09369967925982	36.0446216250755	12.96798967794968
6	30.26224742949268	38.343593728144945	18.478301219724212	12.915857622638164
7	26.538672824380345	32.455846786824324	23.434971254894528	17.5705091339008
8	24.566376775229063	36.99281714839688	21.25188038614219	17.188925690231866
9	25.531959953498273	16.283053708015235	21.179529221933503	37.00545711655299
10-11	24.243480224290842	26.746989222751555	29.186782434928666	19.822748118028937
12-13	24.78067643120174	24.2085419953118	29.378426867851843	21.63235470563462
14-15	21.830769234485782	25.73094995733369	26.662880905632203	25.77539990254833
16-17	21.686137809485764	28.897742820508466	27.38656949736856	22.029549872637215
18-19	21.728264381618825	28.242390080052505	28.846557480676378	21.182788057652296
20-21	22.398195192250046	27.477645556795583	28.701807332367697	21.422351918586674
22-23	22.111623182811154	27.42012068744656	28.55587793660041	21.912378193141876
24-25	21.92801615111129	27.519201277452073	28.63459117751306	21.91819139392358
26-27	21.905963896537926	27.712873791347125	28.519613617964616	21.861548694150333
28-29	21.883385603401294	27.80924494211174	28.49729745076447	21.810072003722503
30-31	22.100264082822033	27.647683292515197	28.469019879345886	21.783032745316884
32-33	21.890642673282535	27.812211927708187	28.395550033786993	21.90159536522229
34-35	21.98298110275709	27.82269936755069	28.396757624073576	21.79756190561864
36-37	21.987267615266386	27.770861592767442	28.39250697295358	21.84936381901259
38-39	21.887373183078473	27.86879798300706	28.41723850245757	21.8265903314569
40-41	22.082601063921654	27.749744464935734	28.298823600771467	21.86883087037115
42-43	21.995119846183652	27.84609240087599	28.149597104645217	22.00919064829514
44-45	22.07967548632073	27.912294658071364	28.00797677120682	22.000053084401088
46-47	22.089791442842436	27.899979218872282	27.853839005743957	22.15639033254132
48-49	22.036136682533037	27.99393500392734	27.85169183398029	22.118236479559332
50-51	22.02648169442885	28.075453381794247	27.786461673160147	22.11160325061676
52-53	22.202212342480635	27.972310373172338	27.66102686028894	22.164450424058085
54-55	22.156158361813887	27.90127917120205	27.725739694891367	22.216822772092694
56-57	22.121165012566195	28.022276356050234	27.706250159660417	22.150308471723154
58-59	22.12019860657291	28.046242350313932	27.682311535038846	22.151247508074317
60-61	22.187499088019937	27.999589061784096	27.71732211281704	22.095589737378926
62-63	22.1633156458359	28.042401114666482	27.65583141444153	22.13845182505609
64-65	22.129327573967732	28.006712886895297	27.6476501934765	22.216309345660466
66-67	22.18479397640732	28.04820641023953	27.534838684071765	22.23216092928138
68-69	22.144198122208273	28.048706107201543	27.57806318933973	22.229032581250458
70-71	22.134398092261172	27.85530894532543	27.65926797226315	22.35102499015024
72-73	22.150659079545846	27.91034549068028	27.647092573570276	22.291902856203595
74-75	22.030094116148366	28.01497182800467	27.60206664409599	22.352867411750978
76-77	22.15693949955564	27.961124724882996	27.53843373739259	22.343502038168776
78-79	22.148518610004594	27.998708139416333	27.497088052669827	22.355685197909242
80-81	22.134149966277974	27.973660488208278	27.53064330057393	22.361546244939817
82-83	22.13766182499796	27.997365938073663	27.59618597596645	22.26878626096193
84-85	22.249074084948237	27.911643842197808	27.57117721731165	22.268104855542305
86-87	22.048673411732185	27.963009182665534	27.63713227672383	22.351185128878445
88-89	22.132632049433838	27.957079953108643	27.58033582549258	22.32995217196494
90-91	22.152807296200493	27.98527300529124	27.62679772687404	22.23512197163423
92-93	22.1260733808415	27.959276590073618	27.61904353351854	22.295606495566343
94-95	21.984806397338364	28.011252728741148	27.667692657173244	22.336248216747247
96-97	22.001790147040765	28.033815434892297	27.704837974056485	22.259556444010457
98-99	22.203628511539602	28.585981738461058	27.822419927074467	21.38796982292487
100-101	26.282737706006966	35.62779456873114	28.391813250076453	9.697654475185441
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2411.0
1	2116.0
2	2781.0
3	4030.5
4	5339.0
5	8388.0
6	11144.5
7	13184.0
8	14610.5
9	14519.0
10	13340.5
11	11867.5
12	12338.5
13	15803.0
14	24414.5
15	36479.0
16	46275.0
17	52272.0
18	54929.0
19	54778.5
20	53373.5
21	52340.0
22	53776.0
23	60534.5
24	73223.0
25	91857.5
26	116249.5
27	143672.0
28	173315.0
29	204288.5
30	233351.0
31	262355.0
32	290731.5
33	316612.5
34	341916.5
35	364904.5
36	388374.5
37	410670.5
38	428891.5
39	445454.0
40	462763.0
41	479843.0
42	493185.0
43	505700.0
44	517245.0
45	525397.0
46	528738.5
47	528603.5
48	525099.0
49	516907.5
50	506357.5
51	491572.0
52	469844.5
53	445274.0
54	419368.0
55	389900.0
56	359488.5
57	326194.0
58	290107.5
59	252044.0
60	212534.0
61	175306.0
62	141339.0
63	111176.5
64	84861.0
65	64820.5
66	50110.0
67	39049.0
68	30449.5
69	22463.0
70	16219.0
71	11995.5
72	8692.5
73	6238.5
74	4612.5
75	3351.0
76	2295.5
77	1568.5
78	1092.0
79	791.0
80	559.0
81	388.0
82	288.5
83	212.0
84	163.5
85	127.5
86	102.5
87	78.0
88	52.0
89	33.0
90	20.5
91	14.0
92	11.0
93	7.5
94	3.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	5.395192625689669E-4
3	1.449454735259911E-4
4	1.6105052613999013E-5
5	0.0
6	0.0
7	8.052526306999506E-6
8	0.0010951435777519327
9	0.0
10-11	2.6975963128448346E-4
12-13	4.267838942709738E-4
14-15	0.0
16-17	0.0
18-19	4.06652578503475E-4
20-21	0.0
22-23	0.0
24-25	1.570991916239545E-4
26-27	4.030051773672131E-6
28-29	4.355483953550215E-4
30-31	3.392189161390264E-4
32-33	0.0010199742116520155
34-35	0.0011853438083627061
36-37	2.4460418456608746E-5
38-39	0.0016327740248134061
40-41	3.7599811488593475E-4
42-43	9.574797002855413E-5
44-45	9.239365070832332E-5
46-47	3.394221422883077E-5
48-49	1.2857556870474086E-5
50-51	2.1641802544002545E-5
52-53	4.369073461076886E-5
54-55	3.087911290484447E-4
56-57	5.751085528539026E-4
58-59	5.184558095768616E-4
60-61	4.742273832753135E-4
62-63	5.533488209519997E-5
64-65	6.994722901254378E-5
66-67	1.604537026650369E-4
68-69	7.647905716618326E-5
70-71	1.6468712739537428E-4
72-73	0.0
74-75	4.972244928807398E-6
76-77	7.709969297692849E-4
78-79	1.5323347924736007E-5
80-81	2.0212961691254785E-4
82-83	1.578164349719747E-5
84-85	2.135289372280442E-5
86-87	1.0839798776479392E-5
88-89	2.7549434291651422E-5
90-91	7.292918968377341E-5
92-93	5.719737441172501E-5
94-95	2.921321280896551E-4
96-97	6.598620888234359E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	476.0
20-21	1341.0
22-23	2662.0
24-25	5509.0
26-27	7548.0
28-29	13556.0
30-31	26497.0
32-33	33469.0
34-35	46403.0
36-37	71642.0
38-39	86610.0
40-41	88612.0
42-43	99947.0
44-45	118780.0
46-47	120059.0
48-49	115805.0
50-51	109238.0
52-53	107173.0
54-55	117314.0
56-57	123794.0
58-59	125471.0
60-61	123497.0
62-63	120144.0
64-65	124988.0
66-67	133655.0
68-69	137245.0
70-71	136665.0
72-73	131490.0
74-75	134806.0
76-77	130189.0
78-79	140586.0
80-81	143697.0
82-83	138683.0
84-85	139374.0
86-87	148059.0
88-89	158966.0
90-91	169333.0
92-93	179010.0
94-95	206357.0
96-97	308088.0
98-99	1065139.0
100-101	7026586.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.78839285657465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.15517235166845	77.34267343757129
2	6.961559233647067	11.944419568608588
3	1.613305530278586	4.152086658876715
4	0.5814161527503657	1.9951502930122635
5	0.2846425656147118	1.220951412132911
6	0.14212066840795134	0.7315382240652184
7	0.08299677316393883	0.49841118474111973
8	0.04609707845306501	0.31636754206975065
9	0.02794383420173125	0.21575309637753962
>10	0.10213392385861889	1.3710952037983049
>50	0.002170042774716309	0.121501768621304
>100	3.969000589075558E-4	0.051918961863568355
>500	1.4983802059788292E-5	0.008368190297312662
>1k	2.996131989989668E-5	0.029764457964101695
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.4157578920998518E-5	0.0	0.0	0.0
3	0.0	2.4157578920998518E-5	0.0	1.610505261399901E-5	0.0
4	0.0	7.247273676299555E-5	0.0	1.610505261399901E-5	0.0
5	0.0	7.247273676299555E-5	0.0	1.610505261399901E-5	0.0
6	0.0	7.247273676299555E-5	0.0	1.610505261399901E-5	0.0
7	0.0	7.247273676299555E-5	0.0	4.026263153499753E-5	0.0
8	0.0	8.857778937699456E-5	0.0	4.8315157841997035E-5	0.0
9	0.0	2.3352326290298567E-4	0.0	6.442021045599604E-5	0.0
10-11	0.0	2.3352326290298567E-4	0.0	1.0065657883749383E-4	0.0
12-13	0.0	2.3352326290298567E-4	0.0	1.0468284199099357E-4	0.0
14-15	0.0	3.140485259729807E-4	0.0	1.1273536829799308E-4	0.0
16-17	0.0	3.2210105227998024E-4	0.0	1.2481415775849233E-4	0.0
18-19	0.0	3.58337420661478E-4	0.0	1.368929472189916E-4	4.026263153499752E-6
20-21	0.0	3.986000521964755E-4	0.0	1.691030524469896E-4	8.052526306999505E-6
22-23	0.0	4.6302026265247157E-4	0.0	2.2547073659598616E-4	8.052526306999505E-6
24-25	0.0	5.354929994154671E-4	0.0	2.939172102054819E-4	1.2078789460499257E-5
26-27	0.0	5.717293677969649E-4	0.0	3.2210105227998024E-4	2.4157578920998518E-5
28-29	0.0	5.717293677969649E-4	0.0	7.16674841322956E-4	2.4157578920998518E-5
30-31	0.0	6.401758414064608E-4	0.0	0.001888317418991384	2.4157578920998518E-5
32-33	0.0	6.522546308669599E-4	0.0	0.004263812679556238	2.818384207449827E-5
34-35	0.0	7.327798939369551E-4	0.0	0.00731974641306255	3.221010522799802E-5
36-37	0.0	7.972001043929511E-4	0.0	0.011857344987056772	3.221010522799802E-5
38-39	0.0	8.213576833139496E-4	0.0	0.019012014610825835	4.026263153499753E-5
40-41	0.0	8.978566832304449E-4	0.0	0.026859201496996853	4.026263153499753E-5
42-43	0.0	9.300667884584429E-4	0.0	0.033530719542345946	4.026263153499753E-5
44-45	0.0	0.0010387758936029363	0.0	0.04153895695465695	4.026263153499753E-5
46-47	0.0	0.0011354062092869303	0.0	0.05001021462962043	4.026263153499753E-5
48-49	0.0	0.00113943247244043	0.0	0.05785337525263795	4.026263153499753E-5
50-51	0.0	0.0012924304722734207	0.0	0.06542677624437099	4.8315157841997035E-5
52-53	0.0	0.0013246405775014186	0.0	0.07296394086772251	4.8315157841997035E-5
54-55	0.0	0.0013407456301154177	0.0	0.08035213375439457	4.8315157841997035E-5
56-57	0.0	0.0013608769458829165	0.0	0.0881791893247981	4.8315157841997035E-5
58-59	0.0	0.0013608769458829165	0.0	0.09568414384292162	4.8315157841997035E-5
60-61	0.0	0.0014051658405714136	0.0	0.10293141751922118	4.8315157841997035E-5
62-63	0.0	0.0014575072615669105	0.0	0.10928083451229029	4.8315157841997035E-5
64-65	0.0	0.0016507678929348986	0.0	0.11573493434735038	4.8315157841997035E-5
66-67	0.0	0.0016789517350093968	0.0	0.12225345439286649	4.8315157841997035E-5
68-69	0.0	0.0017192143665443944	0.0	0.12918667954319307	4.8315157841997035E-5
70-71	0.0	0.0017473982086188926	0.0	0.13616016732505465	4.8315157841997035E-5
72-73	0.0	0.0017473982086188926	0.0	0.14140638821406482	4.8315157841997035E-5
74-75	0.0	0.0017715557875398911	0.0	0.14586346152498905	4.8315157841997035E-5
76-77	0.0	0.0018359759979958874	0.0	0.14974075294180933	4.8315157841997035E-5
78-79	0.0	0.0018681861032238854	0.0	0.15347309888510358	4.8315157841997035E-5
80-81	0.0	0.0018681861032238854	0.0	0.15522452335687598	4.8315157841997035E-5
82-83	0.0	0.001872212366377385	0.0	0.15599756588234792	4.8315157841997035E-5
84-85	0.0	0.0018802648926843845	0.0	0.15685515993404336	4.8315157841997035E-5
86-87	0.0	0.0019003962084518833	0.0	0.15730610140723533	5.2341420995496784E-5
88-89	0.0	0.001972868945214879	0.0	0.15749130951229634	5.636768414899654E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12785	0.0	40.016197	1
GTATCAA	21995	0.0	32.25611	1
TCAACGC	24125	0.0	28.350603	4
ATCAACG	24250	0.0	28.152716	3
CAACGCA	24750	0.0	27.651691	5
AACGCAG	25800	0.0	26.591297	6
TATCAAC	26515	0.0	26.189672	2
ACGCAGA	30655	0.0	22.29801	7
CGCAGAG	30930	0.0	22.072708	8
GTACATG	31585	0.0	22.038528	1
TACATGG	31735	0.0	21.473173	2
ACATGGG	32660	0.0	20.339777	3
CATGGGG	23515	0.0	18.287739	4
GCAGAGT	37685	0.0	17.949543	9
AGAGTAC	32700	0.0	17.155087	10-11
GAGTACT	21110	0.0	17.02221	12-13
CAGAGTA	36150	0.0	16.264265	10-11
GAGTACA	22470	0.0	15.656802	1
ATGGGGG	14705	0.0	15.333363	5
GTACTTT	24740	0.0	14.728493	14-15
>>END_MODULE