FastQCFastQC Report
Fri 27 May 2016
SRR2138825_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138825_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20812322
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA218800.046.6831361
GTATCAA372300.033.033081
TCAACGC436700.027.3267544
ATCAACG441800.027.0397723
CAACGCA450850.026.4504995
AACGCAG466200.025.678596
TATCAAC472100.025.6418362
ACGCAGA554800.021.5248897
GTACATG560200.021.5191361
CGCAGAG563950.021.160788
TACATGG553450.021.0924572
ACATGGG565900.020.1912463
CATGGGG416900.017.9733474
GAGTACT399000.017.43432412-13
GCAGAGT676400.017.4318269
AGAGTAC605150.016.72997510-11
CAGAGTA658100.016.59129310-11
GAGTACA404400.016.4860291
GTGGTAT118650.015.9028451
AGTACAT431750.014.9951652
Produced by FastQC (version 0.11.5)