##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138825_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20812322
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.138038561963437	31.0	31.0	34.0	28.0	34.0
2	31.349468021876657	31.0	31.0	34.0	28.0	34.0
3	31.398916036374988	31.0	31.0	34.0	28.0	34.0
4	35.214929934295654	37.0	35.0	37.0	32.0	37.0
5	34.793338532817245	35.0	35.0	37.0	32.0	37.0
6	34.803730934011114	35.0	35.0	37.0	32.0	37.0
7	34.77042013860827	35.0	35.0	37.0	32.0	37.0
8	34.74297769369511	35.0	35.0	37.0	32.0	37.0
9	36.450206853420774	38.0	35.0	39.0	32.0	39.0
10-11	36.21861849437079	38.0	35.0	39.0	32.0	39.0
12-13	36.23405576754002	38.0	35.0	39.0	32.0	39.0
14-15	37.1960596467804	39.0	36.0	40.0	32.0	41.0
16-17	37.08254242366614	38.0	36.0	40.0	32.0	41.0
18-19	37.12735645258612	38.5	36.0	40.0	32.0	41.0
20-21	37.17132118223016	39.0	36.0	40.0	32.0	41.0
22-23	37.143766066831816	39.0	36.0	40.0	32.0	41.0
24-25	37.08291382471066	39.0	36.0	40.0	31.0	41.0
26-27	36.913137326116626	39.0	36.0	40.0	31.0	41.0
28-29	36.713592357633374	38.5	36.0	40.0	30.5	41.0
30-31	36.58529866942754	38.0	35.0	40.0	30.0	41.0
32-33	36.45623764303368	38.0	35.0	40.0	30.0	41.0
34-35	36.35874684434209	38.0	35.0	40.0	30.0	41.0
36-37	36.29849951373012	38.0	35.0	40.0	30.0	41.0
38-39	36.202848886978515	38.0	35.0	40.0	30.0	41.0
40-41	36.1793482091413	38.0	35.0	40.0	30.0	41.0
42-43	36.11733837477957	38.0	35.0	40.0	30.0	41.0
44-45	36.03680407572983	38.0	35.0	40.0	29.5	41.0
46-47	35.96491479384525	38.0	34.0	40.0	29.0	41.0
48-49	35.974345801573975	38.0	34.0	40.0	29.0	41.0
50-51	35.85072584045878	38.0	34.0	40.0	29.0	41.0
52-53	35.63483903663167	38.0	34.0	40.0	28.0	41.0
54-55	35.41365597370982	38.0	33.5	40.0	28.0	41.0
56-57	35.27993904642321	37.5	33.0	40.0	28.0	41.0
58-59	35.07945609617725	37.0	33.0	39.5	27.0	40.0
60-61	34.90537904522077	37.0	33.0	39.0	27.0	40.0
62-63	34.779363858320544	36.5	33.0	39.0	27.0	40.0
64-65	34.53429557532002	36.0	32.0	39.0	26.0	40.0
66-67	34.42478913359943	36.0	32.0	39.0	26.0	40.0
68-69	34.17981346902382	35.5	32.0	39.0	26.0	40.0
70-71	33.87554280763129	35.0	32.0	38.0	26.0	40.0
72-73	33.601722868721765	35.0	31.0	38.0	26.0	40.0
74-75	33.274663623426726	35.0	31.0	37.0	26.0	39.0
76-77	31.252620654592214	33.0	29.0	35.5	23.5	37.5
78-79	32.53533834164823	34.0	31.0	36.0	26.0	39.0
80-81	32.593656971274356	34.0	31.0	36.0	26.0	38.0
82-83	32.47255910147479	34.0	31.0	35.5	26.0	37.0
84-85	32.282723220021694	34.0	31.0	35.0	26.0	37.0
86-87	32.034424259575886	34.0	31.0	35.0	25.5	36.5
88-89	31.898238008545206	34.0	31.0	35.0	25.0	36.0
90-91	31.74588271667112	34.0	31.0	35.0	25.0	36.0
92-93	31.74757524230919	34.0	31.0	35.0	25.0	35.0
94-95	31.763412343127904	34.0	31.0	35.0	25.0	35.0
96-97	31.864909397033323	34.0	31.0	35.0	26.0	35.0
98-99	32.12148193316913	34.0	31.0	35.0	26.5	35.0
100-101	31.015514201131055	32.5	28.5	34.5	23.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	7.0
19	107.0
20	496.0
21	2527.0
22	9583.0
23	28789.0
24	68609.0
25	135362.0
26	229800.0
27	351402.0
28	493251.0
29	651748.0
30	827291.0
31	1029762.0
32	1279125.0
33	1611481.0
34	2065784.0
35	2677052.0
36	3388615.0
37	3518113.0
38	2081682.0
39	360670.0
40	1064.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.703353522975476	21.525339652154145	14.0879667343221	23.68334009054828
2	15.512486563229078	22.614539523085682	39.53936933039379	22.33360458329145
3	18.798449109569905	26.68486647945047	30.68934421574317	23.827340195236452
4	13.144666867733482	18.59921520518689	38.54335021484151	29.71276771223812
5	12.778742323898316	38.37495402963687	36.064212344975246	12.78209130148957
6	29.904409512787666	38.8886497143375	18.427439283324563	12.779501489550277
7	26.185074399675344	32.72634355743679	23.641129519329944	17.447452523557917
8	24.391212499109894	37.31994253729056	21.32694307822572	16.96190188537383
9	25.49189369643618	16.24647168153558	21.32287305568307	36.93876156634517
10-11	24.111691059753245	27.034003741107988	29.26441493073069	19.589890268408077
12-13	24.671820026732817	24.503596956724493	29.480560880345134	21.34402213619755
14-15	21.725242863338362	25.98572855061535	26.806631667528496	25.482396918517793
16-17	21.5077352134888	29.15749691835195	27.540906476963656	21.793861391195595
18-19	21.573171672171036	28.438141373916476	29.047755757365074	20.940931196547417
20-21	22.269969397887763	27.66588797901844	28.948908623971707	21.115233999122086
22-23	21.99168201060875	27.61188666074416	28.81501849022819	21.5814128384189
24-25	21.774297631276067	27.707700033670747	28.886842036120804	21.631160298932382
26-27	21.779256981417923	27.875838516000513	28.778709030624487	21.566195471957077
28-29	21.78520159686779	27.981634802414824	28.734109727720362	21.499053872997024
30-31	21.96648560927456	27.83923783164331	28.68451567965147	21.509760879430665
32-33	21.819114353828528	27.947122672615716	28.625717601321988	21.608045372233768
34-35	21.87637593486053	27.988382749420172	28.623618929607947	21.511622386111352
36-37	21.903376126776404	27.905644368627776	28.62353265170965	21.567446852886174
38-39	21.78606241010921	28.015730289374247	28.649213969774976	21.54899333074157
40-41	21.983626214518477	27.943049290797806	28.48828422749999	21.58504026718373
42-43	21.89718471906543	28.041322544097184	28.333910332517355	21.72758240432003
44-45	22.0050171279989	28.11704935727014	28.142036020385635	21.73589749434532
46-47	22.022731853075744	28.10187381425125	27.989002924003614	21.88639140866939
48-49	21.957826657947816	28.21318559999437	27.981536118205696	21.847451623852116
50-51	21.95383766811247	28.300397765453006	27.89274468603083	21.853019880403693
52-53	22.101154728071513	28.18227008176285	27.790749809799987	21.92582538036565
54-55	22.0835310636136	28.111358132670976	27.85184540449691	21.953265399218516
56-57	22.068549808209976	28.209827081505306	27.809221658486138	21.91240145179858
58-59	22.043088463206658	28.293256041333485	27.775277564847556	21.888377930612297
60-61	22.10504734088148	28.22828098346396	27.821998912653235	21.844672763001324
62-63	22.07467999669722	28.2958445349995	27.760470603587496	21.869004864715784
64-65	22.065516842508522	28.244021129588788	27.75382598558616	21.93663604231653
66-67	22.09525225467064	28.26235941734313	27.655180557886062	21.987207770100166
68-69	22.080807566521482	28.264678170615426	27.695156448853297	21.959357814009795
70-71	22.03421564104643	28.07076159037801	27.77426142930836	22.1207613392672
72-73	22.08385155310017	28.115849646305463	27.746635060896747	22.053663739697623
74-75	21.961524230152914	28.227567225720097	27.727621032831184	22.083287511295808
76-77	22.060476364786545	28.153902947881114	27.708069083469276	22.077551603863064
78-79	22.051809170636112	28.18276212904665	27.646492617312934	22.118936083004296
80-81	22.025473494284437	28.1831024645347	27.6886151402859	22.102808900894967
82-83	22.07401392265196	28.106183207128627	27.778763583742506	22.04103928647691
84-85	22.14420312114964	28.093871187475738	27.719371239499775	22.042554451874846
86-87	21.98280922395263	28.109737914432042	27.802235752110438	22.105217109504892
88-89	22.03138664172462	28.08248245739146	27.751123263649852	22.135007637234068
90-91	22.01784972325035	28.104750446152227	27.829218889369994	22.04818094122743
92-93	22.018275519215003	28.06139303369809	27.85756652473167	22.062764922355242
94-95	21.854257013327356	28.111265527817604	27.913230259916332	22.121247198938708
96-97	21.802441237899757	28.167468571348646	27.96363297641783	22.066457214333767
98-99	21.95716591439988	28.84229711590966	28.005569180163516	21.19496778952694
100-101	22.827767910804532	31.399834570404035	25.145932277791	20.62646524100043
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2940.0
1	2740.0
2	4468.0
3	6842.0
4	9127.5
5	14827.0
6	20087.0
7	24044.5
8	27177.0
9	27143.5
10	24440.5
11	21268.0
12	21911.5
13	28609.0
14	45466.0
15	69167.0
16	88670.5
17	100037.0
18	104608.5
19	104060.0
20	100860.5
21	98207.0
22	100463.0
23	112148.0
24	134153.0
25	166790.0
26	208875.0
27	254294.5
28	303776.5
29	358903.0
30	409582.0
31	457112.0
32	506374.0
33	551268.0
34	591604.0
35	629081.0
36	669086.5
37	705011.5
38	732469.5
39	755753.5
40	779014.0
41	802074.0
42	817259.5
43	830504.0
44	845354.5
45	856529.5
46	860243.5
47	859066.5
48	853670.5
49	840546.0
50	825011.5
51	802462.0
52	765874.5
53	725783.0
54	684396.5
55	633430.0
56	578724.0
57	523311.5
58	464007.5
59	403358.0
60	342185.5
61	283278.0
62	228572.5
63	179765.5
64	137029.0
65	103347.0
66	78603.0
67	61073.0
68	47718.5
69	35774.5
70	26545.0
71	19883.0
72	14988.0
73	11067.0
74	8150.5
75	6048.5
76	4394.0
77	3065.0
78	2171.0
79	1587.0
80	1157.0
81	870.0
82	682.0
83	490.0
84	348.0
85	266.5
86	204.0
87	155.5
88	109.5
89	74.5
90	58.5
91	50.5
92	42.5
93	24.0
94	8.0
95	4.5
96	4.0
97	4.0
98	2.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.4414537695505578E-4
3	9.609691797003718E-5
4	1.4414537695505575E-5
5	0.0
6	0.0
7	0.0
8	0.0010378467140764016
9	0.0
10-11	1.8498656709232155E-4
12-13	3.6276586533689033E-4
14-15	0.0
16-17	2.4024229492509295E-6
18-19	3.219246751996245E-4
20-21	0.0
22-23	0.0
24-25	1.1538473465249016E-4
26-27	0.0
28-29	3.5625115465693074E-4
30-31	2.3381058170771104E-4
32-33	7.416045413832968E-4
34-35	9.229337034434754E-4
36-37	2.6751569338255914E-5
38-39	0.0014551555660348495
40-41	2.5352144361903386E-4
42-43	9.171167851061028E-5
44-45	1.0247595657868772E-4
46-47	2.01888597165474E-5
48-49	1.0191129019642434E-5
50-51	1.028621628245806E-5
52-53	2.0756400060826632E-5
54-55	2.0424340818147246E-4
56-57	4.7608398703411707E-4
58-59	2.699955351827439E-4
60-61	3.621593236161122E-4
62-63	6.55746842374033E-5
64-65	3.591455275911341E-5
66-67	7.548319096970544E-5
68-69	8.778332492878789E-5
70-71	7.174589326506951E-5
72-73	0.0
74-75	8.845003780207198E-6
76-77	6.786995208261788E-4
78-79	1.5158274363856488E-5
80-81	1.539133216197087E-4
82-83	3.127336413488665E-6
84-85	6.356020810629097E-6
86-87	6.466514046157654E-6
88-89	2.6357436824930444E-5
90-91	3.364899908023826E-5
92-93	1.0335155306378789E-5
94-95	1.663266996111494E-4
96-97	2.5630479590577253E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1044.0
20-21	3060.0
22-23	5707.0
24-25	10810.0
26-27	14866.0
28-29	23842.0
30-31	42261.0
32-33	53749.0
34-35	72972.0
36-37	107196.0
38-39	129627.0
40-41	137605.0
42-43	159051.0
44-45	189061.0
46-47	190243.0
48-49	183651.0
50-51	174178.0
52-53	172574.0
54-55	187703.0
56-57	198252.0
58-59	203787.0
60-61	201660.0
62-63	199802.0
64-65	209413.0
66-67	225256.0
68-69	233334.0
70-71	235196.0
72-73	229457.0
74-75	239716.0
76-77	223655.0
78-79	246817.0
80-81	255258.0
82-83	253767.0
84-85	264354.0
86-87	282141.0
88-89	309077.0
90-91	339657.0
92-93	369289.0
94-95	441031.0
96-97	639780.0
98-99	1865453.0
100-101	1.128697E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.42761378475919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.03848830123499	74.28268613974231
2	7.207932688291482	12.026812490106458
3	2.0214731337844616	5.059400396449107
4	0.7985702373946272	2.6649123738144973
5	0.3570648469307544	1.489453407292657
6	0.1862723499120404	0.9324154600344735
7	0.1260639221010507	0.7362048543666897
8	0.07604829566186296	0.5075622271573664
9	0.04827169096958739	0.3624472791853186
>10	0.13703244708120937	1.689661773670779
>50	0.0021157061900258186	0.11767979732628486
>100	6.260909703750188E-4	0.0832560129796529
>500	1.0077091280375447E-5	0.007246900196539433
>1k	3.0212386145394002E-5	0.04026088767794853
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.9219383594007436E-5	0.0	4.804845898501859E-6	0.0
3	0.0	1.9219383594007436E-5	0.0	4.804845898501859E-6	0.0
4	0.0	6.726784257902602E-5	0.0	9.609691797003718E-6	0.0
5	0.0	7.207268847752787E-5	0.0	9.609691797003718E-6	0.0
6	4.324361308651673E-5	7.207268847752787E-5	0.0	9.609691797003718E-6	4.804845898501859E-6
7	4.324361308651673E-5	7.207268847752787E-5	0.0	9.609691797003718E-6	9.609691797003718E-6
8	4.324361308651673E-5	8.16823802745316E-5	0.0	9.609691797003718E-6	9.609691797003718E-6
9	4.324361308651673E-5	1.633647605490632E-4	0.0	2.882907539101115E-5	9.609691797003718E-6
10-11	4.324361308651673E-5	1.6816960644756504E-4	0.0	4.324361308651673E-5	1.2012114746254646E-5
12-13	4.324361308651673E-5	1.6816960644756504E-4	0.0	4.324361308651673E-5	1.6816960644756505E-5
14-15	4.324361308651673E-5	2.450471408235948E-4	0.0	5.2853304883520445E-5	4.564603603576766E-5
16-17	6.726784257902602E-5	2.4985198672209665E-4	0.0	6.006057373127323E-5	4.804845898501859E-5
18-19	6.726784257902602E-5	2.7147379326535504E-4	0.0	6.246299668052416E-5	6.006057373127323E-5
20-21	6.726784257902602E-5	2.9309559980861337E-4	0.0	6.967026552827694E-5	7.207268847752787E-5
22-23	6.726784257902602E-5	3.171198293011227E-4	0.0	1.0330418681778997E-4	7.927995732528066E-5
24-25	7.207268847752787E-5	3.843876718801487E-4	0.0	1.537550687520595E-4	8.648722617303345E-5
26-27	7.687753437602974E-5	4.08411901372658E-4	0.0	2.810834850623587E-4	8.648722617303345E-5
28-29	7.687753437602974E-5	4.132167472711599E-4	0.0	8.50457724034829E-4	9.369449502078624E-5
30-31	7.687753437602974E-5	4.7087489805318214E-4	0.0	0.0022078266903616042	9.609691797003718E-5
32-33	7.687753437602974E-5	4.732773210024331E-4	0.0	0.004591030256018526	9.609691797003718E-5
34-35	8.16823802745316E-5	5.45350009479961E-4	0.0	0.007738204319537244	9.609691797003718E-5
36-37	8.408480322378252E-5	6.15020275008238E-4	0.0	0.012728036785131423	9.609691797003718E-5
38-39	8.648722617303345E-5	6.390445045007472E-4	0.0	0.020516691986602935	9.609691797003718E-5
40-41	9.129207207153531E-5	6.967026552827696E-4	0.0	0.029532985315141674	9.609691797003718E-5
42-43	9.369449502078624E-5	7.111171929782751E-4	0.0	0.036987703726667306	9.609691797003718E-5
44-45	9.84993409192881E-5	8.096165338975632E-4	0.0	0.04588147348479425	1.0090176386853903E-4
46-47	1.0090176386853903E-4	8.937013371213457E-4	0.0	0.05539506836382793	1.0330418681778997E-4
48-49	1.0090176386853903E-4	8.985061830198476E-4	0.0	0.06536992844911779	1.0570660976704089E-4
50-51	1.0090176386853903E-4	9.946031009898848E-4	2.4024229492509295E-6	0.0745640010759011	1.1051145566554275E-4
52-53	1.0090176386853903E-4	9.994079468883866E-4	4.804845898501859E-6	0.08347458779467279	1.1051145566554275E-4
54-55	1.0090176386853903E-4	9.994079468883866E-4	4.804845898501859E-6	0.09298337782780797	1.1051145566554275E-4
56-57	1.0090176386853903E-4	0.0010090176386853902	4.804845898501859E-6	0.10258586235596392	1.1771872451329554E-4
58-59	1.0090176386853903E-4	0.0010162249075331433	4.804845898501859E-6	0.11210185965794686	1.225235704117974E-4
60-61	1.0330418681778997E-4	0.0010306394452286485	4.804845898501859E-6	0.12094998338003804	1.2732841631029926E-4
62-63	1.153163015640446E-4	0.0010426515599749034	4.804845898501859E-6	0.12977168044968745	1.297308392595502E-4
64-65	1.153163015640446E-4	0.001191601782828461	4.804845898501859E-6	0.13834352553261475	1.321332622088011E-4
66-67	1.1771872451329554E-4	0.0012012114746254646	4.804845898501859E-6	0.1472132710612492	1.3453568515805204E-4
68-69	1.2012114746254647E-4	0.0012444550877119816	4.804845898501859E-6	0.15615749170131038	1.3934053105655388E-4
70-71	1.2732841631029926E-4	0.0012612720483567378	4.804845898501859E-6	0.16429690065337255	1.4414537695505575E-4
72-73	1.561574917013104E-4	0.0012660768942552398	4.804845898501859E-6	0.16990175339397498	1.537550687520595E-4
74-75	1.5855991465056133E-4	0.0012925035466970001	4.804845898501859E-6	0.17469939202362908	1.6096233759981226E-4
76-77	1.5855991465056133E-4	0.0013405520056820185	4.804845898501859E-6	0.17863696323745137	1.633647605490632E-4
78-79	1.5855991465056133E-4	0.0013741859269715316	4.804845898501859E-6	0.18244720603496334	1.6576718349831413E-4
80-81	1.5855991465056133E-4	0.0013741859269715316	4.804845898501859E-6	0.18435953470256705	1.729744523460669E-4
82-83	1.5855991465056133E-4	0.0013741859269715316	4.804845898501859E-6	0.18513311489222586	1.7777929824456878E-4
84-85	1.5855991465056133E-4	0.0013934053105655391	4.804845898501859E-6	0.18599558473100697	1.9219383594007433E-4
86-87	1.6096233759981226E-4	0.0014222343859565502	4.804845898501859E-6	0.18635354575044533	2.0180352773707807E-4
88-89	1.6816960644756504E-4	0.001463075576093816	4.804845898501859E-6	0.18648567901265414	2.0660837363557994E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21880	0.0	46.683136	1
GTATCAA	37230	0.0	33.03308	1
TCAACGC	43670	0.0	27.326754	4
ATCAACG	44180	0.0	27.039772	3
CAACGCA	45085	0.0	26.450499	5
AACGCAG	46620	0.0	25.67859	6
TATCAAC	47210	0.0	25.641836	2
ACGCAGA	55480	0.0	21.524889	7
GTACATG	56020	0.0	21.519136	1
CGCAGAG	56395	0.0	21.16078	8
TACATGG	55345	0.0	21.092457	2
ACATGGG	56590	0.0	20.191246	3
CATGGGG	41690	0.0	17.973347	4
GAGTACT	39900	0.0	17.434324	12-13
GCAGAGT	67640	0.0	17.431826	9
AGAGTAC	60515	0.0	16.729975	10-11
CAGAGTA	65810	0.0	16.591293	10-11
GAGTACA	40440	0.0	16.486029	1
GTGGTAT	11865	0.0	15.902845	1
AGTACAT	43175	0.0	14.995165	2
>>END_MODULE