FastQCFastQC Report
Fri 27 May 2016
SRR2138824_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138824_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8941643
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91250.038.8905331
GTATCAA150450.031.2563971
TCAACGC160800.028.078454
ATCAACG163250.027.7591133
CAACGCA165750.027.2645785
AACGCAG171650.026.4002296
TATCAAC180750.025.5788972
TACATGG232750.022.5306222
ACGCAGA201900.022.444527
GTACATG241050.022.2213781
CGCAGAG204850.022.0806398
ACATGGG237700.021.5704273
CATGGGG166850.019.7460464
CTTATAC64200.018.6201861
GCAGAGT260900.017.3369839
GAGTACT142700.016.56387912-13
AGAGTAC225600.016.20040110-11
GAGTACA168650.016.00391
CAGAGTA248400.015.97096510-11
ATGGGGG106150.015.8127585
Produced by FastQC (version 0.11.5)