##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138824_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8941643
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.474428692802874	34.0	31.0	34.0	31.0	34.0
2	32.68496785210503	34.0	31.0	34.0	31.0	34.0
3	32.79445667871106	34.0	31.0	34.0	31.0	34.0
4	36.244417161365085	37.0	37.0	37.0	35.0	37.0
5	36.092675361787535	37.0	35.0	37.0	35.0	37.0
6	36.13204508388447	37.0	36.0	37.0	35.0	37.0
7	36.12434258446686	37.0	35.0	37.0	35.0	37.0
8	36.08617476676266	37.0	35.0	37.0	35.0	37.0
9	37.960076688367	39.0	38.0	39.0	35.0	39.0
10-11	37.846526583537276	39.0	38.0	39.0	35.0	39.0
12-13	37.87217824509433	39.0	38.0	39.0	35.0	39.0
14-15	39.24264478015953	40.0	39.0	41.0	36.0	41.0
16-17	39.21777815329912	40.0	39.0	41.0	36.0	41.0
18-19	39.24924994209677	40.0	39.0	41.0	36.0	41.0
20-21	39.25061146102005	40.0	39.0	41.0	36.0	41.0
22-23	39.2152141161516	40.0	39.0	41.0	36.0	41.0
24-25	39.225099274459275	40.0	39.0	41.0	36.0	41.0
26-27	39.10529408181205	40.0	39.0	41.0	36.0	41.0
28-29	39.021974026209776	40.0	39.0	41.0	36.0	41.0
30-31	38.931158578681384	40.0	38.0	41.0	35.0	41.0
32-33	38.85429661007521	40.0	38.0	41.0	35.0	41.0
34-35	38.79386231929597	40.0	38.0	41.0	35.0	41.0
36-37	38.76223712929951	40.0	38.0	41.0	35.0	41.0
38-39	38.69834436753567	40.0	38.0	41.0	35.0	41.0
40-41	38.68630874356333	40.0	38.0	41.0	35.0	41.0
42-43	38.66715993337752	40.0	38.0	41.0	35.0	41.0
44-45	38.607157763663906	40.0	38.0	41.0	35.0	41.0
46-47	38.537833818344836	40.0	38.0	41.0	34.0	41.0
48-49	38.509606476529285	40.0	38.0	41.0	34.0	41.0
50-51	38.446060939502345	40.0	38.0	41.0	34.0	41.0
52-53	38.28635979852314	40.0	38.0	41.0	34.0	41.0
54-55	38.15632015582001	40.0	37.0	41.0	34.0	41.0
56-57	38.02195644341221	40.0	37.0	41.0	33.0	41.0
58-59	37.8710595088134	39.5	37.0	41.0	33.0	41.0
60-61	37.75258373932506	39.0	36.0	41.0	33.0	41.0
62-63	37.56515220369448	39.0	36.0	41.0	33.0	41.0
64-65	37.35515105241029	39.0	35.5	40.0	33.0	41.0
66-67	37.139224290382025	38.5	35.0	40.0	32.5	41.0
68-69	36.87397554218993	38.0	35.0	40.0	32.0	41.0
70-71	36.577947308671256	37.0	35.0	40.0	32.0	41.0
72-73	36.265094789173155	37.0	35.0	39.0	32.0	41.0
74-75	35.86852117794207	36.0	34.5	39.0	31.5	40.5
76-77	33.88524170604849	34.5	32.0	36.5	29.0	39.0
78-79	34.98811111756516	35.0	34.0	37.0	30.5	39.0
80-81	34.947937809148044	35.0	34.0	37.0	31.0	39.0
82-83	34.668983599845205	35.0	34.0	36.5	31.0	39.0
84-85	34.3479280874474	35.0	34.0	36.0	31.0	37.5
86-87	34.159812845122765	35.0	34.0	36.0	31.0	37.0
88-89	33.96148700548089	35.0	34.0	35.5	30.5	37.0
90-91	33.788417736229405	35.0	34.0	35.0	30.0	36.0
92-93	33.70772049833839	35.0	34.0	35.0	30.0	36.0
94-95	33.63469033966941	35.0	34.0	35.0	30.0	36.0
96-97	33.653154069295425	35.0	34.0	35.0	30.5	36.0
98-99	33.686252665736596	35.0	34.0	35.0	31.0	35.0
100-101	32.459957294265934	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	10.0
20	32.0
21	150.0
22	555.0
23	1932.0
24	4941.0
25	11012.0
26	20675.0
27	34758.0
28	52904.0
29	74984.0
30	101609.0
31	136508.0
32	182380.0
33	249957.0
34	370167.0
35	578816.0
36	1056354.0
37	2087031.0
38	2757881.0
39	1199123.0
40	19859.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.08027540352483	20.29350758020646	13.155333980567105	24.470883035701604
2	14.718939824856461	21.5235622435963	41.02490575930166	22.73259217224558
3	18.356011305752197	26.187088882882037	30.43106283710947	25.0258369742563
4	12.141198880619466	17.88967780567058	39.38509897919492	30.584024334515032
5	12.710431760880809	38.22824643778037	36.30033989381785	12.760981907520968
6	29.76809519549074	38.43598461125526	18.99439697593272	12.801523217321284
7	26.33677054653155	32.51274961436058	23.61922747307178	17.531252366036085
8	24.256436988146362	37.03716419901801	21.72625321766928	16.980145595166345
9	25.425875311729623	16.397981892142194	21.37717866839461	36.798964127733576
10-11	23.935411656967727	26.914672614735586	29.503114846106914	19.646800882189773
12-13	24.60097132634606	24.315831818688135	29.592998469623073	21.490198385342737
14-15	21.61642803505374	25.916963904222733	26.876530444661643	25.590077616061883
16-17	21.496362581239264	29.09464737073489	27.60510009178403	21.80388995624182
18-19	21.515739170355904	28.4366124646244	28.990017944326173	21.057630420693517
20-21	22.244137925326775	27.70522084463711	28.78919257099418	21.261448659041925
22-23	21.867118092023215	27.72464171747439	28.69793536653703	21.710304823965362
24-25	21.6943349291332	27.791814457333704	28.81781524803661	21.696035365496492
26-27	21.745896505781936	28.0254385391137	28.666639323747305	21.562025631357056
28-29	21.577741520788773	28.141024819141347	28.655823424419918	21.625410235649962
30-31	21.996531518567693	27.899919211553204	28.642675037850136	21.460874232028974
32-33	21.570836790820678	28.147284070382884	28.607702777768097	21.674176361028348
34-35	21.73301228649325	28.161855701265825	28.656932622121644	21.44819939011928
36-37	21.693611930175795	28.116981250974714	28.607581350350536	21.581825468498952
38-39	21.554417722787285	28.091583834096788	28.73730535737683	21.616693085739097
40-41	21.76542634451413	27.978020286530885	28.67555003249766	21.58100333645732
42-43	21.74336696124908	28.017795925058714	28.530864393903947	21.707972719788255
44-45	21.76881432226887	28.16195071643236	28.368787964911885	21.700446996386884
46-47	21.8407831859501	28.05962767446271	28.146247548215875	21.95334159137131
48-49	21.79130277961107	28.102912567341487	28.215747931791324	21.89003672125612
50-51	21.700376723690585	28.252234323371933	28.17883872034433	21.86855023259315
52-53	21.85469305403487	28.17875016883868	28.156334270094618	21.81022250703183
54-55	21.82004477163127	28.127177172160412	28.111844930274803	21.940933125933515
56-57	21.902838163533144	28.28079850847619	27.95934984281797	21.857013485172693
58-59	21.753770190003742	28.369519032678735	28.107630781969366	21.76907999534815
60-61	21.93320940718716	28.375133293278555	27.97840504613246	21.713252253401823
62-63	21.82253630092709	28.461413146478424	27.95109078022654	21.764959772367938
64-65	21.786200364028815	28.399140891473213	27.970039841391102	21.84461890310687
66-67	21.91279727483584	28.356968298112896	27.80749154040895	21.92274288664231
68-69	21.8996434108872	28.296224782387213	27.93172854413642	21.872403262589167
70-71	21.820573132920725	28.129696188653377	27.996193120323102	22.053537558102796
72-73	21.8959624357026	28.14986953228703	27.9936583027735	21.960509729236865
74-75	21.762552543004375	28.24390459128861	27.97755830680212	22.01598455890489
76-77	21.860319431023065	28.21475911596128	27.861581403160944	22.06334004985471
78-79	21.877367051852616	28.23911857662833	27.85681893093923	22.02669544057983
80-81	21.84817724469059	28.256617662325738	27.84304768898617	22.052157403997498
82-83	21.89497029770846	28.2136679740787	27.962653433275225	21.928708294937614
84-85	22.05607685130695	28.134915754013353	27.861129117589083	21.947878277090613
86-87	21.82571811839345	28.22076704619181	27.941613237056583	22.01190159835816
88-89	21.897136951114483	28.22073173905324	27.89057005822678	21.9915612516055
90-91	21.9433764773364	28.206285759194603	27.942737586894378	21.907600176574615
92-93	21.96449571899225	28.160907618627295	27.955919636000093	21.918677026380358
94-95	21.8312446854257	28.270584983074194	27.96322072093918	21.934949610560928
96-97	21.837967247557398	28.234498831847628	28.0687561128445	21.858777807750478
98-99	22.100499362651835	28.793989964330997	28.114157644971705	20.991353028045463
100-101	25.964479360316652	35.789031088975676	28.849457193137717	9.397032357569952
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1092.0
1	1048.0
2	1650.0
3	2511.0
4	3647.0
5	6091.5
6	8071.0
7	9255.5
8	9822.0
9	9394.5
10	8426.5
11	7523.5
12	8136.0
13	10809.0
14	16857.5
15	24925.0
16	31523.0
17	35951.0
18	37529.0
19	36875.0
20	35806.0
21	35525.0
22	37418.5
23	43055.5
24	53143.0
25	67349.5
26	85759.5
27	106262.0
28	128748.0
29	152749.0
30	175049.5
31	195965.0
32	217867.0
33	239397.0
34	259575.0
35	279838.5
36	301082.5
37	318630.0
38	331267.5
39	342466.5
40	355020.5
41	366344.5
42	373219.0
43	379729.5
44	386004.5
45	390044.0
46	393770.0
47	396680.0
48	392388.0
49	382804.5
50	371490.5
51	353948.0
52	331566.0
53	309410.0
54	287187.5
55	264037.5
56	241113.5
57	216141.0
58	189079.0
59	163463.0
60	138588.5
61	113888.0
62	90451.0
63	70395.5
64	53720.0
65	40723.5
66	30990.5
67	23863.0
68	18363.0
69	13472.5
70	9877.0
71	7279.5
72	5229.5
73	3775.5
74	2806.0
75	1982.5
76	1379.0
77	948.0
78	647.5
79	445.0
80	305.5
81	212.5
82	159.0
83	120.0
84	84.0
85	58.5
86	46.0
87	37.5
88	26.5
89	18.5
90	12.0
91	6.5
92	4.5
93	2.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0019795019774330063
3	0.0
4	0.003008395660618524
5	3.0195792876096706E-4
6	4.80895960619318E-4
7	0.0
8	0.0
9	0.0
10-11	3.299169962388344E-4
12-13	6.15099484513081E-4
14-15	1.6775440486720393E-5
16-17	0.0
18-19	0.001095995445132399
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.476328586914271E-5
28-29	0.0
30-31	3.364081599836102E-5
32-33	0.0010904948868156246
34-35	7.838877346439635E-4
36-37	3.400164703978261E-4
38-39	0.001895400496697693
40-41	0.0
42-43	1.7415565998057933E-5
44-45	2.3449600360185863E-5
46-47	1.7786418551661424E-5
48-49	5.995502533729348E-6
50-51	3.0303708004136578E-5
52-53	0.0
54-55	0.0034833541148713665
56-57	0.003016502144219844
58-59	0.0015455253494027431
60-61	1.667165106285623E-4
62-63	6.4904210147890094E-6
64-65	9.85245359592881E-5
66-67	5.322063686075945E-5
68-69	1.6852327636752258E-4
70-71	0.0
72-73	0.0
74-75	1.7508467970533948E-4
76-77	0.0
78-79	3.521036179149746E-4
80-81	0.0
82-83	8.864804200853414E-5
84-85	2.9944936502136456E-5
86-87	0.001790777405306195
88-89	0.0
90-91	2.0331845433244647E-4
92-93	4.768638761069501E-5
94-95	2.4248949414266627E-4
96-97	8.241805337788743E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	205.0
20-21	627.0
22-23	1227.0
24-25	2805.0
26-27	4455.0
28-29	9756.0
30-31	21320.0
32-33	27072.0
34-35	37317.0
36-37	60462.0
38-39	72679.0
40-41	71812.0
42-43	80241.0
44-45	94142.0
46-47	94977.0
48-49	90976.0
50-51	85292.0
52-53	82908.0
54-55	90038.0
56-57	95776.0
58-59	95474.0
60-61	95791.0
62-63	91008.0
64-65	93854.0
66-67	99834.0
68-69	97229.0
70-71	94045.0
72-73	88871.0
74-75	90036.0
76-77	89814.0
78-79	94365.0
80-81	96116.0
82-83	90010.0
84-85	88878.0
86-87	93709.0
88-89	99131.0
90-91	102766.0
92-93	104094.0
94-95	111982.0
96-97	170863.0
98-99	715895.0
100-101	5213791.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.81219475196608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.59831856385952	78.65038875366436
2	6.699494178822481	11.631955867832005
3	1.5858304019776064	4.130082531002058
4	0.5473579477078554	1.900693790218032
5	0.24965442375164437	1.0836524227708808
6	0.10707121481572619	0.5577052291747452
7	0.0708604369079745	0.43060850343451407
8	0.03960116991117486	0.2750291579787689
9	0.025971145367027885	0.20291509165806415
>10	0.0742834792743367	0.9851658628585441
>50	0.0011862422527943726	0.07030243898511979
>100	3.3281908685658766E-4	0.0500337176713893
>500	1.8989899146067762E-5	0.008473571790161552
>1k	1.8986365939688976E-5	0.022993060961357826
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.236725398229386E-5	0.0
5	0.0	0.0	0.0	3.3550880973440786E-5	0.0
6	0.0	0.0	0.0	3.3550880973440786E-5	0.0
7	0.0	1.118362699114693E-5	0.0	5.5918134955734646E-5	0.0
8	0.0	1.118362699114693E-5	0.0	6.710176194688157E-5	0.0
9	0.0	7.82853889380285E-5	0.0	6.710176194688157E-5	0.0
10-11	0.0	7.82853889380285E-5	0.0	8.387720243360197E-5	0.0
12-13	0.0	7.82853889380285E-5	0.0	9.506082942474891E-5	0.0
14-15	0.0	1.3420352389376317E-4	0.0	1.0065264292032237E-4	0.0
16-17	0.0	1.56570777876057E-4	0.0	1.0065264292032237E-4	0.0
18-19	0.0	1.7893803185835088E-4	0.0	1.1183626991146929E-4	0.0
20-21	0.0	1.901216588494978E-4	0.0	1.6775440486720394E-4	0.0
22-23	0.0	2.0689709933621817E-4	0.0	2.1248891283179164E-4	0.0
24-25	0.0	2.348561668140855E-4	0.0	2.7399886128309976E-4	0.0
26-27	0.0	2.4603979380523246E-4	0.0	3.6346787721227517E-4	0.0
28-29	0.0	2.4603979380523246E-4	0.0	6.98976686946683E-4	0.0
30-31	0.0	2.9077430176982017E-4	0.0	0.0019683183504418597	0.0
32-33	0.0	2.9077430176982017E-4	0.0	0.004199451935175672	0.0
34-35	0.0	3.8583513119456905E-4	0.0	0.007012134123449124	0.0
36-37	0.0	4.3616145265473023E-4	0.0	0.011675706578757394	0.0
38-39	0.0	4.585287066370241E-4	0.0	0.01906249220640994	0.0
40-41	0.0	5.42405909070626E-4	0.0	0.027813680326982414	0.0
42-43	0.0	5.759567900440669E-4	0.0	0.03515573144667038	0.0
44-45	0.0	6.766094329643893E-4	0.0	0.04377830785684465	0.0
46-47	0.0	7.493030084068442E-4	0.0	0.05335149256126642	0.0
48-49	0.0	7.493030084068442E-4	0.0	0.06271218835285641	0.0
50-51	0.0	8.164047703537259E-4	0.0	0.07184361979112788	0.0
52-53	0.0	8.275883973448728E-4	0.0	0.08022574822099249	0.0
54-55	0.0	8.275883973448728E-4	0.0	0.08896575271457383	0.0
56-57	0.0	8.387720243360196E-4	0.0	0.09726959575550041	0.0
58-59	0.0	8.387720243360196E-4	0.0	0.10510931827629441	0.0
60-61	0.0	8.499556513271666E-4	0.0	0.11299936711854858	0.0
62-63	0.0	8.499556513271666E-4	0.0	0.12050917264310373	0.0
64-65	0.0	9.058737862829013E-4	0.0	0.12815877350504823	0.0
66-67	0.0	9.058737862829013E-4	0.0	0.1354169474223026	0.0
68-69	0.0	9.28241040265195E-4	0.0	0.14242348973225616	0.0
70-71	0.0	9.39424667256342E-4	0.0	0.1492566858238469	0.0
72-73	0.0	9.562001077430625E-4	0.0	0.15480935662495138	0.0
74-75	0.0	9.897509887165032E-4	0.0	0.1592045220324721	0.0
76-77	0.0	0.0010288936831855174	0.0	0.16277769085614355	0.0
78-79	0.0	0.0010512609371678113	0.0	0.16640118600127515	0.0
80-81	0.0	0.0010512609371678113	0.0	0.16807313823645165	0.0
82-83	0.0	0.0010736281911501054	0.0	0.1685204833160975	0.0
84-85	0.0	0.001095995445132399	0.0	0.16895664476875222	0.0
86-87	0.0	0.0011071790721235459	0.0	0.16923064363003532	0.0
88-89	0.0	0.0011407299530969868	0.0	0.16935366352693795	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9125	0.0	38.890533	1
GTATCAA	15045	0.0	31.256397	1
TCAACGC	16080	0.0	28.07845	4
ATCAACG	16325	0.0	27.759113	3
CAACGCA	16575	0.0	27.264578	5
AACGCAG	17165	0.0	26.400229	6
TATCAAC	18075	0.0	25.578897	2
TACATGG	23275	0.0	22.530622	2
ACGCAGA	20190	0.0	22.44452	7
GTACATG	24105	0.0	22.221378	1
CGCAGAG	20485	0.0	22.080639	8
ACATGGG	23770	0.0	21.570427	3
CATGGGG	16685	0.0	19.746046	4
CTTATAC	6420	0.0	18.620186	1
GCAGAGT	26090	0.0	17.336983	9
GAGTACT	14270	0.0	16.563879	12-13
AGAGTAC	22560	0.0	16.200401	10-11
GAGTACA	16865	0.0	16.0039	1
CAGAGTA	24840	0.0	15.970965	10-11
ATGGGGG	10615	0.0	15.812758	5
>>END_MODULE