FastQCFastQC Report
Fri 27 May 2016
SRR2138823_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138823_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7177383
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64450.039.235121
GTATCAA110900.032.4141161
TCAACGC122650.028.5343764
ATCAACG124400.028.165793
CAACGCA126300.027.676655
AACGCAG131000.026.7782926
TATCAAC137750.025.8860442
ACGCAGA153300.022.8561577
GTACATG185200.022.6449581
CGCAGAG156750.022.3794638
TACATGG184800.022.3138792
ACATGGG188400.021.11993
CATGGGG135150.019.5360764
GCAGAGT199700.017.6490159
AGAGTAC173300.016.7730510-11
ATGGGGG90400.016.088695
GAGTACA130800.016.0789071
GAGTACT108100.015.72916812-13
CAGAGTA192350.015.43409210-11
GTGGTAT41600.015.0972111
Produced by FastQC (version 0.11.5)